[Objective]To explore the background of the creation of the Yijian Fang series of works from the perspective of the simple and frugal social atmosphere in the Song Dynasty,in order to enrich and improve the research on the Yijian Fang series of works.[Methods]By sorting out the development of the Yijian Fang series of works,this paper analyzes the simple and frugal social atmosphere in the Song Dynasty from the perspectives of tombs,diet,clothing,and literature,and explores the intrinsic connection between the Yijian Fang series of works and the simple and frugal social atmosphere in the Song Dynasty,thereby supplementing and improving the background of the creation of the Yijian Fang series of works.[Results]As the mainstream reference of prescriptions in the Southern Song Dynasty during the Song,Jin and Yuan Dynasties,the Yijian Fang series of works is a group of medical books that focus on the reform and simplification of prescriptions to better fit clinical practice.Its origin can be traced back to CHEN Yan's Sanyin Jiyi Bingzheng Fanglun,and it was eventually abandoned and forgotten by the times due to ZHU Danxi's systematic criticism of Hejiju Fang.One of the major characteristics of the Song Dynasty compared to the Han and Tang Dynasties was the advocacy of frugality and simplicity,which was reflected in many aspects such as tombs,diet,clothing and literature.Benefiting from the overall trend of simplicity in society,the medical field in the Song Dynasty integrated and reformed the vast number of prescriptions and medicines from the Han and Tang Dynasties,thus giving birth to the Yijian Fang series of works.The Yijian Fang series of works is the result of the influence of the simple trend in the Song Dynasty on the medical field,and it is the active response of folk doctors in the Southern Song Dynasty to the trend of"simplification"in medical books.[Conclusion]Exploring the background of the creation of the Yijian Fang series of works from the perspective of the simple and frugal social atmosphere in the Song Dynasty not only directly supplements and improves the research on the Yijian Fang series of works,but also demonstrates the potential role of social ideology and culture in the development of medicine.At the same time,it also has enlightening significance for the exploration of the source and background of the thoughts of other medical practitioners in the Song Dynasty.

[Objective]To explore the background of the creation of the Yijian Fang series of works from the perspective of the simple and frugal social atmosphere in the Song Dynasty,in order to enrich and improve the research on the Yijian Fang series of works.[Methods]By sorting out the development of the Yijian Fang series of works,this paper analyzes the simple and frugal social atmosphere in the Song Dynasty from the perspectives of tombs,diet,clothing,and literature,and explores the intrinsic connection between the Yijian Fang series of works and the simple and frugal social atmosphere in the Song Dynasty,thereby supplementing and improving the background of the creation of the Yijian Fang series of works.[Results]As the mainstream reference of prescriptions in the Southern Song Dynasty during the Song,Jin and Yuan Dynasties,the Yijian Fang series of works is a group of medical books that focus on the reform and simplification of prescriptions to better fit clinical practice.Its origin can be traced back to CHEN Yan's Sanyin Jiyi Bingzheng Fanglun,and it was eventually abandoned and forgotten by the times due to ZHU Danxi's systematic criticism of Hejiju Fang.One of the major characteristics of the Song Dynasty compared to the Han and Tang Dynasties was the advocacy of frugality and simplicity,which was reflected in many aspects such as tombs,diet,clothing and literature.Benefiting from the overall trend of simplicity in society,the medical field in the Song Dynasty integrated and reformed the vast number of prescriptions and medicines from the Han and Tang Dynasties,thus giving birth to the Yijian Fang series of works.The Yijian Fang series of works is the result of the influence of the simple trend in the Song Dynasty on the medical field,and it is the active response of folk doctors in the Southern Song Dynasty to the trend of"simplification"in medical books.[Conclusion]Exploring the background of the creation of the Yijian Fang series of works from the perspective of the simple and frugal social atmosphere in the Song Dynasty not only directly supplements and improves the research on the Yijian Fang series of works,but also demonstrates the potential role of social ideology and culture in the development of medicine.At the same time,it also has enlightening significance for the exploration of the source and background of the thoughts of other medical practitioners in the Song Dynasty.

To study the expression of herpes simplex virus type 2 latency-associated transcript (LAT) open reading frame 1 (ORF1) and its anti-apoptosis function induced by actinomycin D in Vero cells. The recombinant plasmid pEGFP-ORF1 was constructed and transfected into Vero cells, and the expression of ORF1 was identified by RT-PCR. The changes of Vero cells morphology induced by actinomycin D were observed by fluorescence microscopy, Hochest33258 fluorescence staining. Cells viability was evaluated by MTT assay and cells apoptosis rate was detected by flow cytometry. Double digestion and sequencing confirmed the pEGFP-ORF1 was constructed successfully, RT-PCR showed that the target gene was highly expressed in Vero cells. Hochest33258 staining reaveals that Vero cells transfected with pEGFP-ORF1 and induced apoptosis by actinomycin D had no changes in morphology. MTT assay showed that the viabilities of Vero cells transfected with recombinant plasmid pEGFP-ORF1 and induced apoptosis by actinomycin D has no statistically significant difference compared with the untreated normal control group (P > 0.05), but remarkable higher than Vero cells transfected with empty plasmid pEGFP-C2 and induced apoptosis by actinomycin D, the difference was statistically significant (P < 0.05). Flow cytometry assay shows that the cells apoptosis rate had no significant difference between pEGFP-ORF1 group and the normal group, but the cells apoptosis rate ofpEGFP-ORF1 was lower than the pEGFP-C2 group. HSV-2 LAT ORF1 gene can be expressed in Vero cells and can protect Vero cells from apoptosis induced by actinomycin D.
Animals ; Apoptosis ; physiology ; Cercopithecus aethiops ; Dactinomycin ; Herpes Simplex Virus Protein Vmw65 ; genetics ; Herpesvirus 2, Human ; genetics ; Open Reading Frames ; genetics ; Promoter Regions, Genetic ; Transcription, Genetic ; Vero Cells ; Viral Proteins ; genetics ; Virus Activation ; Virus Latency ; genetics ; physiology