Objective To explore the clinical application value of CT-guided facial nerve radiofrequency thermocoagulation therapy for hemifacial spasm(HFS).Methods A total of 24 patients with HFS,who were admitted to the Department of Intervention and Pain of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine of China from July 2017 to June 2024 to receive CT-guided facial nerve radiofrequency thermocoagulation therapy,were enrolled in this study.The preoperative and the postoperative one-day,one-week,one-month,and 3-month HFS scores were used to evaluate the therapeutic effect.The postoperative one-day facial paralysis score was used to assess the degree of facial paralysis.Results Successful intraoperative induction of facial nerve motor stimulation symptoms was achieved in all patients.The minimum voltage for inducing facial muscle twitching through sport stimulation was(0.38±0.12)voltage,and the final radiofrequency temperature was(59.04±7.00)℃.The preoperative and the postoperative one-day,one-week,one-month,and 3-month HFS scores were(6.83±1.09)points,(0.71±0.75)points,(0.50±0.72)points,(0.38±1.06)points,and(0.38±1.28)points respectively.All the postoperative one-day,one-week,one-month,and 3-month HFS scores were significantly lower than the preoperative HFS score,the differences were statistically significant(all P＜0.05).The symptoms of HFS were obviously improved in all patients.The postoperative one-day facial paralysis score was(2.67±1.13)points.After treatment,all 24 patients developed mild facial paralysis symptoms.Conclusion In treating HFS,CT-guided facial nerve radiofrequency thermocoagulation can effectively alleviate the spasm symptoms,therefore,it is an effective method for HFS.The complication of facial paralysis is mild,and it can recover spontaneously.In clinical practice this therapy can be recommended to HFS patients.

Objective To assess the clinical application value of radiofrequency ablation (RFA) by using different-depth needle-puncturing through medial wall of oval foramen under fluoroscopic guidance in treating primary trigeminal neuralgia.Method A total of 32 patients with primary trigeminal neuralgia were enrolled in this study.Guided by fluoroscopic monitoring,RFA by using different-depth needle-puncturing through medial wall of oval foramen was carried out in all patients.The intraoperative exact replication rates of responsible nerve were recorded,and the postoperative one-day,one-week,3-month and one-year cure rates were calculated.Results During the operation,the precise replication rates the neuralgia of branch Ⅰ,branch Ⅱ and branch Ⅲ of the trigeminal nerve were 85.7％(6/7),96.4％ and 100％ respectively.The postoperative one-day,one-week,3-month and one-year cure rates were 87.5％,93.8％,93.8％ and 87.5％ respectively.Conclusion In treating trigeminal neuralgia with RFA,fluoroscopy-guided needle-puncturing through medial wall of oval foramen can accurately replicate the pain symptoms of the dominating region of responsible nerve,thus,the trigeminal neuralgia can be precisely treated.Being minimally-invasive and safe with reliable effectiveness,this technique is worthy of clinical application.

BACKGROUND:Navigation assisted minimal y invasive posterior lumbar interbody fusion and pedicle screw fixation can precisely real-time guide a variety of operation under minimal y invasive sleeve, and implant the pedicle screws and interbody fusion cage and other implants safely and accurately, thus can determine the decompression parts. Minimal y invasive transforaminal lumbar interbody fusion is the typical approach in recent years for the successful application of minimal y invasive spine surgery techniques with the advantages of smal incision, less bleeding, slight tissue damage and faster recovery. OBJECTIVE:To evaluate the short-term effect of minimal y invasive transforaminal lumbar interbody fusion with real-time three-dimensional navigation system and open posterior transforaminal lumbar interbody fusion. METHODS:Forty cases with single-level lumbar disc herniation were retrospectively analyzed. The patients were treated with minimal y invasive transforaminal lumbar interbody fusion with real-time three-dimensional navigation system (20 cases) and open posterior transforaminal lumbar interbody fusion (20 cases) respectively for the comparative analysis. The fusion duration, intraoperative blood loss, postoperative drainage volume, the length of postoperative hospital stay and the length of hospital stay were compared between two groups. The wound pain and function were evaluated after treatment with visual analogue scale score and Japanese Orthopaedic Association score. RESULTS AND CONCLUSION:Al patients were fol owed-up for 7.7 months. The operative duration in the minimal y invasive transforaminal lumbar interbody fusion group was longer than that in the open posterior transforaminal lumbar interbody fusion group, and the difference was significant (P<0.01);the length of hospital stay and length of postoperative hospital stay of the minimal y invasive transforaminal lumbar interbody fusion group were shorter than the open posterior transforaminal lumbar interbody fusion group, and the differences were significant (P<0.01);the intraoperative blood loss of the minimal y invasive transforaminal lumbar interbody fusion group was less than the open posterior transforaminal lumbar interbody fusion group, and the difference was significant (P<0.05);the postoperative drainage volume of the minimal y invasive transforaminal lumbar interbody fusion group was less than the open posterior transforaminal lumbar interbody fusion group, and the difference was significant (P<0.01). The visual analogue scale score was significantly decreased and the Japanese Orthopaedic Association score was significantly increased in the minimal y invasive transforaminal lumbar interbody fusion group at 3 days after internal fixation compared with open posterior transforaminal lumbar interbody fusion group (P<0.01), but there were no significant differences in the visual analogue scale score and Japanese Orthopaedic Association score between the two groups before operation and 6 months after operation (P>0.05).The results indicate that minimal y invasive transforaminal lumbar interbody fusion with real-time three-dimensional navigation system is an effective method for lumbar disc herniation with the advantages of less intraoperative blood loss, less postoperative drainage volume, smal trauma, short hospital stay and short-term efficacy.

AIM:To study the induction of apoptosis by c-myc antisense oligonucleotide in osteosarcoma cell(MG-63).METHODS:The designed c-myc antisense oligonucleotide fragment was transfected into human osteosarcoma MG-63 cells.The cell growth and apoptosis were measured by the methods of MTT,FCM,HE staining and transmission electron microscopy.RESULTS:The results showed that the proliferation of human osteosarcoma MG-63 cells was inhibited and apoptotic rate was 37.92% when treated with c-myc antisense oligonucleotide at the does of 10.0 ?mol/L for 48 h.c-myc antisense oligonucleotide(10.0 ?mol/L) also inhibited the expression of c-myc protein.CONCLUSION:c-myc antisense oligonucleotide is able to induce apoptosis in human osteosarcoma MG-63 cells.