The diagnosis of occupational radiation diseases, with the diagnostic criteria as main technical basis, has always been one of the emphases of China's occupational health work. This study compared the diagnostic standards for occupational radiation diseases between China and the International Labour Organization (ILO) across three aspects—types, classifications/grades, and diagnostic dose requirements—categorizing the diseases into tissue reactions and stochastic effects. ① For tissue reactions, 8 types are included both in China and ILO standards, 13 unique to China's standards, and 4 unique to the ILO standards; significant differences exist in classifaton/grading; China's standards are either consistent with or stricter (i.e., requiring higher diagnostic dose values) than the ILO standards. ② For stochastic effects (radiation-induced cancer), 12 types were included in both standards; China's standards provide more comprehensive coverage for certain malignancies (e.g., bone cancer); while 5 types were unique to the ILO standards have, some (e.g. salivary gland cancer) are primarily observed in non-occupational populations. Overall, China’s standards include more disease types and maintain more stringent diagnostic dose requirements. However, specific discrepancies require further deliberation during future revisions. Finally, based on these results, recommendations were provided for basic research and the evaluation of Chinese diagnostic criteria.

The diagnosis of occupational radiation diseases, with the diagnostic criteria as main technical basis, has always been one of the emphases of China's occupational health work. This study compared the diagnostic standards for occupational radiation diseases between China and the International Labour Organization (ILO) across three aspects—types, classifications/grades, and diagnostic dose requirements—categorizing the diseases into tissue reactions and stochastic effects. ① For tissue reactions, 8 types are included both in China and ILO standards, 13 unique to China's standards, and 4 unique to the ILO standards; significant differences exist in classifaton/grading; China's standards are either consistent with or stricter (i.e., requiring higher diagnostic dose values) than the ILO standards. ② For stochastic effects (radiation-induced cancer), 12 types were included in both standards; China's standards provide more comprehensive coverage for certain malignancies (e.g., bone cancer); while 5 types were unique to the ILO standards have, some (e.g. salivary gland cancer) are primarily observed in non-occupational populations. Overall, China’s standards include more disease types and maintain more stringent diagnostic dose requirements. However, specific discrepancies require further deliberation during future revisions. Finally, based on these results, recommendations were provided for basic research and the evaluation of Chinese diagnostic criteria.

Background: High-mobility group box protein 1 (HMGB1) is a chromatin-binding protein involved in arthritis, ischemia, sepsis, atherosclerosis, neurodegenerative disorders, meningitis, and cancer. HMGB1 exhibits dual roles in cancer, acting as either a tumor suppressor or oncoprotein depending on context. Objective: This research aimed to elucidate HMGB1’s functional significance in cutaneous squamous cell carcinoma (cSCC). Methods: We overexpressed HMGB1 in cSCC cell lines using recombinant adenovirus and examined its effects on cell proliferation, colony formation, and cell migration. Results: Immunohistochemical analysis revealed elevated HMGB1 expression levels in cSCC tissue relative to normal epidermis. To assess the influence of HMGB1, we employed recombinant adenoviruses expressing HMGB1 to transduce SCC cell lines (SCC12 and SCC13). Enhanced HMGB1 expression significantly promoted cellular proliferation and colony formation capacity.Notably, HMGB1 overexpression elevated the levels of proliferation regulators, including P63, SOX2, CDK4 and CDK6. Furthermore, HMGB1 overexpression substantially enhanced tumor invasiveness, accompanied by upregulation of epithelial-mesenchymal transition (EMT) biomarkers. Mechanistically, overexpression of HMGB1 enhanced transforming growth factor-β signaling by increasing phosphorylation of SMAD2/3, the key mediators of EMT. Conclusion These data imply that HMGB1 acts as a tumor-promoting factor in cSCC.

Objective This study reports on the obligatory parasitism of water mites Arrenurus sp. on Anopheles messeae at the Manzhouli Port, Inner Mongolia, China. Methods Duing July 2024, a survey on the mosquito diversity was conducted at the Manzhouli Port. Captured mosquitoes and their ectoparasites were identified to species level. Results A total of 1840 adult mosquitoes were collected, representing species from three genera: Culex(Cx. modestus, Cx. pipiens pallens), Aedes(Ae. dorsalis, Ae. flavidorsalis, Ae. flavescens), and Anopheles (An. messeae). Among all the mosqutioes specimens,3 out of 150 captured An. messeae were found to carry ectoparasitic mites, with number of 2,4,27 mites separately. Morphological and molecular identification reached the same result as water mites(Hydrachnidiae, Hydracrina). COI gene sequence showed 94% similarity with the closest species Arrenurus truncatellus. Conlusions Literature review suggests water mites are host-specific parasitism of mosquito species and herein with the first record of Arrenurus sp. parasiting on An. Messeae in the most high-latitude region globally.

Caenorhabditis elegans,as an emerging model organism,has been widely used in multiple disciplines such as medicine,life science,and environmental science in recent years,due to its charac-teristics of short life cycle,clear genetic background,highly conserved evolution,complete genome analysis and excellent fitting between experimental data and human results.It also shows unique advan-tages in the field of toxicology.This paper summarizes its advantages in toxicological research starting from the biological characteristics of C.elegans,introduces the toxicological research methods and progress based on the C.elegans model,focuses on demonstrating its applications in environmental fo-rensic medicine and forensic toxicology,and looks forward to the application of the relevant results in the field of forensic science.

Aim To perform high-throughput screen-ing of immunomodulatory traditional Chinese medicine(TCMs)based on an in vitro B cell differentiation-antibody secretion model,identifying active herbal candidates with immune-enhancing properties to pro-vide novel therapeutic options and theoretical support for influenza virus treatment in immunocompromised in-dividuals.Methods B cells were stimulated with dif-ferent concentrations of cytosine-phosphate-guanine oli-godeoxynucleotide 2006(CpG)and nterleukin-2(IL-2)to promote proliferation,differentiation,and anti-body secretion,and the effects of varying concentra-tions of the solvent DMSO were also evaluated.The op-timal conditions for the B cell differentiation-anti-body secretion model were determined based on the se-cretion levels of three antibody isotypes.The feasibility of the model was further validated using rapamycin,a known B cell function inhibitor.On this basis,a high-throughput screening platform for immunomodulatory a-gents was optimized and established.Subsequently,the immune-enhancing activity of 465 polarity extract from TCMs was evaluated.Results The optimal con-ditions for the model were determined as 2 mg·L-1 CpG,1.67 × 106 nkat·L-1 IL-2,and DMSO with a volume fraction of 0.1％.Rapamycin effectively inhib-ited B cell differentiation into plasmablast and signifi-cantly reduced antibody production,indicating the reli-ability of the model.Multiple rounds of screening re-vealed that the dichloromethane extract of licorice,the dichloromethane extract of Vinegar-processed Curcumae Rhizoma,the cyclohexane extract of Honey-prepared Radix Asteris,and the aqueous extract of Siphonostegia chinensis Benth were identified to significantly promote both B cell proliferation and differentiation and anti-body secretion at a concentration of 600 μg·L-1.Conclusion This study successfully optimizes an in vitro B cell differentiation-antibody secretion model and identifies several TCM extracts,including licorice,with potential immune-enhancing activity.

Receptor-interacting protein kinase 1 (RIPK1) plays an essential role in regulating the necroptosis and apoptosis in cerebral ischemia-reperfusion (I/R) injury. However, the regulation of RIPK1 kinase activity after cerebral I/R injury remains largely unknown. In this study, we found the downregulation of protein arginine methyltransferase 1 (PRMT1) was induced by cerebral I/R injury, which negatively correlated with the activation of RIPK1. Mechanistically, we proved that PRMT1 directly interacted with RIPK1 and catalyzed its asymmetric dimethylarginine, which then blocked RIPK1 homodimerization and suppressed its kinase activity. Moreover, pharmacological inhibition or genetic ablation of PRMT1 aggravated I/R injury by promoting RIPK1-mediated necroptosis and apoptosis, while PRMT1 overexpression protected against I/R injury by suppressing RIPK1 activation. Our findings revealed the molecular regulation of RIPK1 activation and demonstrated PRMT1 would be a potential therapeutic target for the treatment of ischemic stroke.

Objective:To explore the effect of empowerment-authorized health management on the rehabilitation,self-efficacy and quality of life in patients with non-ST-segment elevation acute coronary syndrome(NSTE-ACS)after per-cutaneous coronary intervention(PCI).Methods:This randomized controlled study enrolled 100 NSTE-ACS patients ad-mitted in the First Affiliated Hospital of Xinjiang Medical University between January 2021 and June 2023.Patients were randomly divided into control group(n=50,routine nursing)and intervention group(n=50,empowerment-authorized health management).Both groups were intervened for 6 months.The scores of heart health self-efficacy and self-man-agement(HH-SESM)scale,China questionnaire of quality of life in patients with cardiovascular diseases(CQQC),cardi-ac function indexes,serum levels of N terminal pro brain natriuretic peptide(NT-proBNP)and cardiac troponin I(cTnI),6min walking distance(6MWD),satisfaction and incidence of adverse events were compared between the two groups.Re-sults:Compared to patients in the control group after nursing,those in the intervention group had significant higher HH-SESM total score[(79.34±6.18)points vs.(62.33±6.17)points],CQQC total score[(116.24±7.13)points vs.(83.27±7.11)points],left ventricular ejection fraction(LVEF)[(59.32±4.36)％vs.(51.22±4.63)％],6min walk-ing distance(6MWD)[(363.61±12.51)m vs.(299.13±13.54)m]and satisfaction(86.96％vs.68.09％)(P＜0.05 or＜0.01),and significant lower left ventricular end-systolic diameter(LVESd)[(32.44±3.91)mm vs.(39.25±3.61)mm],left ventricular end-diastolic diameter(LVEDd)[(41.23±4.18)mm vs.(46.17±5.41)mm],NT-proBNP[(6.16±1.58)pg/ml vs.(12.51±3.27)pg/ml]and cTnI[(2.65±0.62)ng/ml vs.(5.60±1.81)ng/ml](P＜0.001 all).The incidence of adverse events in the intervention group(4.35％)was significantly lower than that of control group(19.15％,P=0.027).Conclusion:Empowerment-authorized health management mode was beneficial to the recovery of cardiac function and significantly improved self-efficacy and quality of life in patients with non-ST-segment elevation a-cute coronary syndrome.

Aim To investigate the effect of triptolide(TP)on corticosterone(CORT)-induced depression-like behaviors in mice and explore the antidepressant mechanism of TP based on the CREB/BDNF/TrkB sig-naling pathway.Methods Sixty 8-week-old male C57BL/6J mice were randomly divided into five groups:control group,CORT group,TP groups of low and high doses(10,30 μg·kg-1),and fluoxetine(FLU)group(10 mg·kg-1).Except for the control group,the other groups received subcutaneous injec-tions of CORT for three consecutive weeks to establish the model of depression.During the last two weeks of modeling,normal saline,TP and FLU were adminis-tered via intraperitoneal injection respectively.After the administration,depression-like behaviors in mice were assessed using forced swimming test,tail suspen-sion test,and sucrose preference test.Biochemical methods were used to measure the levels of SOD and MDA in the hippocampus and prefrontal cortex(PFC).Cell apoptosis was detected by TUNEL meth-od.Immunohistochemistry,immunofluorescence,and Western blotting were employed to detect the expres-sion of apoptosis/autophagy-related proteins,synaptic structure markers,and proteins related to the CREB/BDNF/TrkB signaling pathway.Results TP signifi-cantly ameliorated CORT-induced depression-like be-haviors in mice,mainly manifested by reduced immo-bility time in the tail suspension test and forced swim-ming test,and increased sucrose preference rate.TP alleviated CORT-induced oxidative stress by increasing SOD levels and reducing MDA production in brain tis-sue.Additionally,TP also inhibited apoptosis and ex-cessive autophagy of neurons in the hippocampus and prefrontal cortex,maintained synaptic plasticity,and significantly upregulated the expression of p-CREB,BDNF,and TrkB.Conclusions TP exhibits potential antidepressant effect in mice by upregulating the CREB/BDNF/TrkB signaling pathway,reducing oxida-tive stress,inhibiting excessive neuronal apoptosis and autophagy,and improving synaptic plasticity.

Objective:To explore the effect of early cardiac rehabilitation strategies on functional prognosis in patients with acute coronary syndrome(ACS)who received coronary artery bypass grafting(CABG).Methods:A total of 158 ACS patients who underwent CABG in the First Affiliated Hospital of Xinjiang Medical University between Jan-uary 2022 and December 2023 were included in this randomized control trial.Participants were randomly divided in-to early cardiac rehabilitation group(n=79)and control group(n=79).Patients in the control group received con-ventional nursing measures,while those in the early rehabilitation group received additional early cardiac rehabilita-tion strategies.Both groups were continuously intervened for 1 month.We compared the changes in cardiac function[left ventricular end-diastolic diameter(LVEDd),left ventricular ejection fraction(LVEF)and 6-miniute walk distance(6MWD)],myocardial injury markers[cardiac troponin I(cTnI),N-terminal pro brain natriuretic pep-tide(NT-proBNP)and cystatin C(CysC)],quality of life[China questionnaire of quality of life in patients with cardiovascular diseases(CQQC)score],ability of daily living(ADL)score,and incidence of major adverse cardio-vascular events(MACE)during the intervention period between two groups.Results:Compared to participants in the control group,those in the early rehabilitation group had significantly higher LVEF[(53.31±1.73)％vs.(52.09±1.72)％,P＜0.001],6MWD[(407.60±25.00)m vs.(395.17±19.20)m,P＜0.001],CQQC score[(84.75±8.44)points vs.(75.98±7.60)points,P＜0.001]and ADL score[(88.67±2.58)points vs.(78.87±3.05)points,P＜0.001],and significantly lower LVEDd[(47.02±2.28)mm vs.(52.93±2.57)mm,P＜0.001],cTnI[(0.48±0.04)μg/L vs.(0.52±0.05)μg/L,P＜0.001],NT-proBNP[(213.74±23.54)ng/ml vs.(287.26±41.99)ng/ml,P＜0.001],CysC[(0.87±0.08)mg/L vs.(1.06±0.10)mg/L,P＜0.001]and in-cidence of MACE(9.21％vs.24.32％,P=0.013).Conclusion:Early cardiac rehabilitation strategy may improve cardiac function and prognosis in patients with acute coronary syndrome who received coronary artery bypass grafting.