The cobalt sulphide nickel ternary material(NiCo2S4)prepared by hydrothermal method in this work was combined with multi-walled carbon nanotubes(MWCNT)for constructing an electrochemical sensor for quantitative detection of rutin.The morphology and crystal of the electrochemical materials were characterized by scanning electron microscopy and X-ray diffractometer,and the electrochemical behavior of the glassy carbon electrode(GCE)modified with NiCo2S4/MWCNT composites was investigated.The experimental results showed that the electrochemical redox process of two electrons and two protons occurred on the surface of modified electrode NiCo2S4/MWCNT/GCE.Under the optimized conditions,the linear range for detection of rutin by square-wave voltammetry(SWV)using this sensor was 0.06-14.8 μmol/L and the detection limit was 14.3 nmol/L.The electrochemical sensor was sensitive,simple and low-cost,with good reproducibility and reliable stability,and could be used for detection of rutin in real samples such as compound rutin tablets and color chrysanthemi tablets,with spiked recoveries of 99.5%-102.0%.

Gray mold (Botrytis cinerea) is one of the most common diseases of strawberries (Fragaria × ananassa Duchesne) worldwide. Although many chemical fungicides are used for controlling the growth of B. cinerea, the risk of the fungus developing chemical resistance together with consumer demand for reducing the use of chemical fungicides have necessitated an alternative method to control this pathogen. Various naturally occurring microbes aggressively attack plant pathogens and benefit plants by suppressing diseases; these microbes are referred to as biocontrol agents. However, screening of potent biocontrol agents is essential for their further development and commercialization. In this study, 24 strains of yeast with antagonistic ability against gray mold were isolated, and the antifungal activity of the volatile and diffusible metabolites was evaluated. Putative mechanisms of action associated with the biocontrol capacity of yeast strains against B. cinerea were studied through in vitro and in vivo assays. The volatile organic compounds produced by the Galactomyces candidum JYC1146 could be useful in the biological control of plant pathogens and therefore are potential alternative fungicides with low environmental impact.
Botrytis* ; Fragaria* ; Fruit* ; Fungi ; In Vitro Techniques ; Mass Screening* ; Methods ; Plants ; Volatile Organic Compounds ; Yeasts*

OBJECTIVETo investigate the risk factors for recurrent wheezing in infants and young children suffering from dust mite allergy after their first wheezing.

METHODSA total of 1 236 infants and young children who experienced a first wheezing episode and were hospitalized between August 2014 and February 2015 were enrolled, among whom 387 were allergic to dust mites. These infants and young children were followed up to 1 year after discharge. A total of 67 infants and young children who experienced 3 or more recurrent wheezing episodes within 1 year were enrolled as the recurrent wheezing group, while 84 infants and young children who did not experience recurrent wheezing during follow-up were enrolled as the control group. Univariate analysis and multivariate logistic stepwise regression analysis were performed to investigate the risk factors for recurrent wheezing in these patients.

RESULTSThe univariate analysis showed that the age on admission, wheezing time before admission, Mycoplasma pneumoniae infection rate, and influenza virus infection rate were associated with recurrent wheezing. The multivariate logistic stepwise regression analysis showed that the older age on admission (OR=2.21, P=0.04) and Mycoplasma pneumoniae infection (OR=3.54, P=0.001) were independent risk factors for recurrent wheezing.

CONCLUSIONSInfants and young children who are allergic to dust mites, especially young children, have a significantly increased risk of recurrent wheezing if they are complicated by Mycoplasma pneumoniae infection during the first wheezing episode.

Animals ; Child, Preschool ; Female ; Humans ; Hypersensitivity ; complications ; Infant ; Logistic Models ; Male ; Pyroglyphidae ; immunology ; Recurrence ; Respiratory Sounds ; etiology ; Risk Factors

Objective To explore the relationship between surgical treatment combined with drug therapy and the recovery of hepatic function following tumor resection of primary liver cancer ( PLC) , and to provide reasonable recommendations for clinical treat-ment of the disease .Methods One hundred and twenty cases , which were diagnosed as primary liver cancer and given tumor resection from January 2009 to December 2010 in our hospital were selected and analyzed retrospectively .There were 82 cases that had complete records and could be used for effective analysis .Based on "the China National Formulary"(2010) and the mechanism of drugs , hepa-to-protectants were divided into 6 categories:⑴A=anti-inflammatory drug , B=membrane system stabilizing drug , C=combined de-toxification drug, D=enhanced energy supply drug, E=regeneration promotion drug and F =the category of other drugs.Results (1) When the A+D treatment profile was compared with the C +D treatment profile, the k value of total bilirubin (TB) was P=0.046 and the k value of aspartate aminotransferase (AST) was P=0.035.The C+D treatment profile was superior to that of the A +D treatment profile.(2) Positive correlation could be noted between the k values of ALT and AST (P<0.01).(3) The k value of TB and AST were negatively correlated with the blocking time of portal vein , cirrhosis type and other variables .Conclusion After tumor resection of the primary liver cancer , the C +D treatment profile was superior to the A +D treatment profile in enhancing recovery of hepatic function .

Objective To explore the relationship between surgical treatment combined with drug therapy and the recovery of hepatic function following tumor resection of primary liver cancer ( PLC) , and to provide reasonable recommendations for clinical treat-ment of the disease .Methods One hundred and twenty cases , which were diagnosed as primary liver cancer and given tumor resection from January 2009 to December 2010 in our hospital were selected and analyzed retrospectively .There were 82 cases that had complete records and could be used for effective analysis .Based on "the China National Formulary"(2010) and the mechanism of drugs , hepa-to-protectants were divided into 6 categories:⑴A=anti-inflammatory drug , B=membrane system stabilizing drug , C=combined de-toxification drug, D=enhanced energy supply drug, E=regeneration promotion drug and F =the category of other drugs.Results (1) When the A+D treatment profile was compared with the C +D treatment profile, the k value of total bilirubin (TB) was P=0.046 and the k value of aspartate aminotransferase (AST) was P=0.035.The C+D treatment profile was superior to that of the A +D treatment profile.(2) Positive correlation could be noted between the k values of ALT and AST (P<0.01).(3) The k value of TB and AST were negatively correlated with the blocking time of portal vein , cirrhosis type and other variables .Conclusion After tumor resection of the primary liver cancer , the C +D treatment profile was superior to the A +D treatment profile in enhancing recovery of hepatic function .

Hematologic Diseases ; Medicine, Chinese Traditional ; standards ; Terminology as Topic

Essential oil, flavonoids, alkaloids and lignas were isolated from Saururuaceae plants. They possesses antibacteral, antiviruses, antiinflammatory. Inhibitory of plaleted aggregation activites and exhibit significant neuroleptic activity.
Alkaloids ; chemistry ; isolation & purification ; pharmacology ; Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Antiviral Agents ; pharmacology ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Lactams ; Oils, Volatile ; isolation & purification ; pharmacology ; Phenanthrenes ; Plants, Medicinal ; chemistry ; Platelet Aggregation Inhibitors ; pharmacology ; Quercetin ; isolation & purification ; pharmacology ; Saururaceae ; chemistry