Puerariae Lobatae Radix and Puerariae Thomsonii Radix are the dried roots of Pueraria lobata and Pueraria thomsonii of the Leguminosae family， respectively. Since their separate inclusion in the 2005 edition of Pharmacopoeia of the People's Republic of China， the two herbs have been individually recorded. Although they share the same functions and indications， the required content of puerarin differs by a factor of up to eightfold between them. The current quality standard relies solely on a single indicator， puerarin， which fails to reflect the correlation between chemical constituents and core therapeutic effects. This has resulted in prominent issues of mixed use in clinical practice and production， thereby constraining the high-quality development of the industry. Modern research has demonstrated that Puerariae Lobatae Radix contains a comprehensive range and higher content of isoflavonoid constituents， while Puerariae Thomsonii Radix is rich in starch and polysaccharides. Puerariae Lobatae Radix exhibits stronger effects in antipyresis， cardiovascular and cerebrovascular protection， and central analgesia， while Puerariae Thomsonii Radix demonstrates greater advantages in anti-inflammation， hypoglycemia， and peripheral analgesia. Based on this， the chemical material bases of Puerariae Lobatae Radix and Puerariae Thomsonii Radix at both the macromolecule and micromolecule levels were systematically reviewed and compared. The pharmacodynamic material bases and mechanisms of action underlying their three core therapeutic effects， namely "relieving exterior syndrome and clearing heat"， "promoting fluid production to quench thirst"， and "dredging channels and activating collaterals"， were summarized. The most highly correlated common metabolic pathway shared by both herbs for these three therapeutic effects is the arachidonic acid metabolic pathway， and their common core target is prostaglandin-endoperoxide synthase 2 （PTGS2）. This review provided clinical application recommendations based on the therapeutic differences between the two herbs， elucidated the scientific connotation of their "same origin but different effects"， and， grounded in the quality marker （Q-Marker）， proposed the concept of efficacy-related Q-Marker based on "substance-efficacy-syndrome" association， as well as a novel strategy for efficacy-oriented quality evaluation. Preliminary screening of quality markers associated with different therapeutic effects was also conducted. This study aims to provide critical scientific evidence for the precise clinical application of Puerariae Lobatae Radix and Puerariae Thomsonii Radix， the improvement of quality standards， and the high-quality development of the industry.

Objective To investigate the surgical nursing coordination methods in the modified outflow tract trans-sternal approach for left ventricular assist device implantation. Methods A retrospective analysis was conducted on the clinical data of 3 patients who underwent modified outflow tract trans-sternal LVAD implantation at our hospital. Results All 3 surgeries were successfully completed. The mean operative time was (311.7±34.1) minutes, and the mean blood loss was (383.3±28.9) mL. Patients were discharged after (48.3±16.8) days postoperatively in average. No significant complications occurred, and the postoperative condition of patients was stable. Conclusion The modified outflow tract for left ventricular assist device implantation provides more stable hemodynamics for patients. Compared with the traditional median sternotomy, the trans-sternal approach not only facilitates the dissection of chest wall tissues and better access to the surgical field but also offers the advantages of smaller incisions and faster recovery. Proficient surgical operation techniques and skilled surgical coordination are crucial for the success of this procedure.

Objective To investigate the current status of regulatory focus among the nurses in operating room,analyse the influencing factors and summarise nursing strategies to provide a reference for development of the programs in targeted nursing management.Methods A total of 681 operating room nurses from 18 hospitals of Grade ⅡA and above in Jiangsu Province were selected by convenience sampling method from Arprial to July 2023.The nurses who participated in the study were surveyed with a general information questionnaire,the nurse regulatory focus scale(NRFQ),spiritual climate scale(SCS),calling scale-12(CS-12),and perceived stress scale(CPSS).Results All 681 operating room nurses completed the study.The promotive focus score was lower than the defensive focus(23.57±2.64 vs.24.06±2.72).It was found that the promotive focus and defensive focus were positively correlated with spiritual climate and occupational call,and negatively correlated with perceived stress(P<0.01).Spiritual climate,occupational call and perceived stress were the influencing factors that affected the promotion focus,jointly explaining 28.00％of the variance(all P<0.05).While occupational call and perceived stress among the operating room nurses were the influencing factors to the defensive focus,jointly explaining 23.60％of the variance(both P<0.05).Conclusion This study has identified that spiritual climate of nurses in operating room positively influences promotive focus in the regulatory focus.The regulatory focus as a whole is positively regulated by occupational call and negatively influenced by perceived stress.Therefore,nursing administrators should create a favourable spiritual climate to enhance the sense of professional call,reduce the perceived stress and increase the promotive and defensive focus among the nurses in operating room,hence to increase the nursing efficiency,thereby improving the quality of nursing care.

Objective To investigate the current status of regulatory focus among the nurses in operating room,analyse the influencing factors and summarise nursing strategies to provide a reference for development of the programs in targeted nursing management.Methods A total of 681 operating room nurses from 18 hospitals of Grade ⅡA and above in Jiangsu Province were selected by convenience sampling method from Arprial to July 2023.The nurses who participated in the study were surveyed with a general information questionnaire,the nurse regulatory focus scale(NRFQ),spiritual climate scale(SCS),calling scale-12(CS-12),and perceived stress scale(CPSS).Results All 681 operating room nurses completed the study.The promotive focus score was lower than the defensive focus(23.57±2.64 vs.24.06±2.72).It was found that the promotive focus and defensive focus were positively correlated with spiritual climate and occupational call,and negatively correlated with perceived stress(P<0.01).Spiritual climate,occupational call and perceived stress were the influencing factors that affected the promotion focus,jointly explaining 28.00％of the variance(all P<0.05).While occupational call and perceived stress among the operating room nurses were the influencing factors to the defensive focus,jointly explaining 23.60％of the variance(both P<0.05).Conclusion This study has identified that spiritual climate of nurses in operating room positively influences promotive focus in the regulatory focus.The regulatory focus as a whole is positively regulated by occupational call and negatively influenced by perceived stress.Therefore,nursing administrators should create a favourable spiritual climate to enhance the sense of professional call,reduce the perceived stress and increase the promotive and defensive focus among the nurses in operating room,hence to increase the nursing efficiency,thereby improving the quality of nursing care.

Objective:The study aimed to investigate the effects of exercise on the expression of ferroptosis-related proteins and the associated mechanisms in a post-traumatic stress disorder(PTSD)model induced by transient foot shock.Methods:Thirty-five male C57BL/6J mice(8 weeks old)were randomly divided into three groups:Control group,PTSD group,and Exe+PTSD group.PTSD model mice were established by foot-shock and PTSD behaviors were assessed using a fear conditioning box.Iron distribution areas were detected by Fe3+staining.Western Blot analysis were performed to determine the expression level of ferroptosis-related proteins in the mice.Results:Compared to the control group,the freezing time and Fe3+distribution areas in the cortex and hippocampus were significantly increased in the mice with PTSD(P＜0.05).The levels of ferroportin1(FPN1),glutathione peroxidase 4(GPX4),and brain-derived neurotrophic factor(BDNF)proteins were significantly decreased(P＜0.05),while the expression of high mobility group box 1(HMGB1)protein was significantly elevated(P＜0.05).Exercise significantly reduced freezing time and Fe3+accumulation in the cortex and hippocampus(P＜0.05),and it also up-regulated the expression of FPN1,GPX4,and BDNF proteins(P＜0.05),while down-regulating HMGB1 expression compared to the PTSD group(P＜0.05).Conclusion:This study demonstrates that PTSD induces the ferroptosis-related pathway in mice,and exercise can inhibit iron accumulation and reverse the expression of ferroptosis-related proteins induced by PTSD,there-by alleviating PTSD-like behavior in mice.

This paper reviewed the teaching objects, teachers arrangement, internship cycle and teaching methods on practice in operating room, to analyze the difficult point of teaching for interns in operating room, so as to understand the clinical teaching of interns in operating room and to provide a reference for interns teaching in operating room.

ObjectiveTo establish the mechanism of selecting and eliminating the clinical instructors of operating room nurses in training base,and explore its application effect.Methods The competitive mechanism was introduced to the operating room of Northern Jiangsu People′s Hospital since 2015. The related competitive qualification and assessment system were established to select and eliminate the clinical instructors of operating room nurses in training base. Then,we analyzed the general information (e.g. education,age, professional title) of instructors and assessed their work between the pre-competitive group (2014) and the post-competitive group (2015).Results Totally 11 nurses were considered to be eligible including five previous instructors and six new outstanding instructors. Nine previous instructors were excluded because of age and other drawbacks. The ecce prance rates for post-competitive instructors on the aspect of theoretical level,specialty skills,scientific research ability,innovation ability,and training effectiveness were 94.29%,97.14%,100.00%, 88.57% and 100.00%. They were all higher than the acceptance rates of 75.86%,82.76%,86.21%,65.52% and 82.76% for pre-competitive instructors (χ2=4.454,3.862,5.149,4.932,6.546;P<0.05).Conclusions The establishment of the mechanism of selecting and eliminating the clinical instructors of operating room nurses in training base can effectively maintain the vitality of instructors. It can also improve the quality of teaching and the competitiveness of development for training base.

This article was aimed to study the effect of polyphenols fromRubus suavissirnusS. Lee (RSLP) on spontaneous hypertensive rats (SHR) and to explore its mechanism of anti-hypertensive. The water extraction of RSLP was prepared. And the polyphenols was extracted with macroporous resin. The non-invasive blood pressure analysis system was used to detect the blood pressure. SHR model was selected to study the anti-hypertensive effect. The 16 normal Wistar rats were randomly divided into the control group and the normal RSLP high-dose group (RSLP-NH). The 40 SHR were randomly divided into the model group, Captopril group, RSLP-L group, RSLP-M group and RSLP-H group. SBP, DBP, HR, body weight and organ index were observed after the drug administration for 8 weeks and drug withdrawal for 2 weeks. The contents of SOD, MDA, GSH-Px, NO, NOS and ANP in serum were measured. The results showed that the blood pressure of SHR was significantly higher than that of the control group, which can be used for anti-hypertensive studies. Each RSLP group can obviously reduce the SBP and DBP of SHR (P < 0.05), but it had no effect on HR (P > 0.05). RSLP can elevate GSH-Px, SOD levels and reduce the activity of MDA (P < 0.05). RSLP can reduce NO, NOS and ANP contents in serum (P < 0.05). It was concluded that RSLP can significantly reduce the SBP and DBP of SHR, but it had no significant effect on HR. It can increase the activity of GSH-Px, SOD, NO, NOS levels, and reduce the contents of MDA, ANP in serum. It had certain inhibitory effect on the left ventricular hypertrophy.

Objective To identify the needs of different degree hospitals for specialist nurse in operating room, and to explore the specialist nurse training programs which can meet their actual requirements . Methods We used the self-designed questionnaire to carry out a survey in students which were graduated from the training courses for specialist nurses in operating room in Jiangsu province .Four points of the contents were as below:the general information , the refinement situation of the third-degree subject and subspecialty of one′s own hospital , how their jobs were going after the traing , and the feasibility and plan of the training for specialist nurses in operating room under the direction of third-degree subject .Results 100%of participants thought that it was necessary to hold a training for specialist nurses in operating room , 80.00%of them thought that the current mode belonged to the general nursing , 72.22% of whom from the second-class hospital thought that both the theoretical knowledge and skill learned from the clinical practice could meet their working requirements , and they would continue to take the current training mode .74.19%of whom from the tertiary hospital thought that although it could meet the requirements , it was not strong enough and was short in details for their own direction , they thought specialist nurses should be trained according to the third-degree subject .Conclusions The training mode is combined with centralization and decentralization , and it includes unified entrance , synchronous training , hierarchical culture .It is established based on the actual needs getting from the hospitals in different grades , so it is better to adapt for different needs of different hospitals .