Aim To explore the correlation between methylation rate of different cytosine-phosphate-guanine(CpG)sites in the Furin promoter region and blood lipids in non-diabetic adults.Methods This study was a cross-sectional study.The study population was non-diabetic adults from the Gusu cohort.The methylation rates of the eight CpG sites in the Furin promoter region,blood glucose,blood lipids,blood pressure and other assays in this population at baseline were collected,and the correlation between the methylation rates of these sites and blood lipids were investigated by variance analysis and Logistic regression models.The E-value was used to evaluate the robustness of the relationship between methylation rates of the CpG sites and blood lipids.Results The methylation rates of CpG sites in Furin pro-moter region were only correlated with the level of total cholesterol(TC).The methylation rate of CpG4 site was lower in TC increased group than that in normal group(P＜0.05).Multivariate Logistic regression analysis showed that methylation rate at CpG2 site(with a change of 10％)was independently correlated with elevated blood TC(OR=1.62,95％CI:1.05～2.51,P=0.031).After adjusting for relevant covariates,the methylation rate of CpG2 was still posi-tively correlated with elevated TC in subgroups of different ages/genders.The E-value calculated according to the correla-tion between the methylation rate of CpG2 and the risk of elevated TC in the multifactorial analysis was 2.62,which suppor-ted the robustness of this relationship.Conclusion Elevated methylation rate of CpG2 site in the Furin promoter re-gion is an independent risk factor for elevated blood TC.This site may be a potential target for regulating blood TC.

Aim To explore the correlation between methylation rate of different cytosine-phosphate-guanine(CpG)sites in the Furin promoter region and blood lipids in non-diabetic adults.Methods This study was a cross-sectional study.The study population was non-diabetic adults from the Gusu cohort.The methylation rates of the eight CpG sites in the Furin promoter region,blood glucose,blood lipids,blood pressure and other assays in this population at baseline were collected,and the correlation between the methylation rates of these sites and blood lipids were investigated by variance analysis and Logistic regression models.The E-value was used to evaluate the robustness of the relationship between methylation rates of the CpG sites and blood lipids.Results The methylation rates of CpG sites in Furin pro-moter region were only correlated with the level of total cholesterol(TC).The methylation rate of CpG4 site was lower in TC increased group than that in normal group(P＜0.05).Multivariate Logistic regression analysis showed that methylation rate at CpG2 site(with a change of 10％)was independently correlated with elevated blood TC(OR=1.62,95％CI:1.05～2.51,P=0.031).After adjusting for relevant covariates,the methylation rate of CpG2 was still posi-tively correlated with elevated TC in subgroups of different ages/genders.The E-value calculated according to the correla-tion between the methylation rate of CpG2 and the risk of elevated TC in the multifactorial analysis was 2.62,which suppor-ted the robustness of this relationship.Conclusion Elevated methylation rate of CpG2 site in the Furin promoter re-gion is an independent risk factor for elevated blood TC.This site may be a potential target for regulating blood TC.

Objective To explore the influence of bacteria lipopolysaccharide on the cytokine production of flour mite allergic rhinitis patients. Methods 8 cases with perennial allergic rhinitis were selected. Then the heparin anticoagulation venous blood was taken from the same patient and was added to fetal calf serum,with ultimate volume 1 ml. The two experimental groups were added 10μg LPS and 1 μg LPS respectively, forming the high LPS dose group and low LPS dose group,while the control group was added nothing. Results The contents of IL-4、IL-5、IL-10、IL-12、IFN-γwere tested by enzyme-linked immunosorbent assay. The findings were that there was significant difference in the productive contents of IL-4、IL-5、IL-10、IL-12 and no significant difference in that of IFN-γ between the high LPS dose group and the low LPS dose group. When compared in two groups,significant difference was found in the productive contents of IL-4 ,IL-10 and IL-12 between the two experimental groups and between the high LPS dose group and the control group, while no obvious difference existed between the low LPS group and control group. However, significant difference was altogether found in the productive content of IL-5 when compared one with another among the three groups,and the result was that low dose of LPS obviously promoted the production of cytokine while high dose of LPS restrained the production of cytokine; no significant difference was found in the production of IFN-γ in all comparisons between the three groups. Conclusion This research, through the experiments in vitro, proved that LPS could effectively adjust the cytokine production of perennial flour mite allergic rhinitis patients and the balance of Th1/Th2 ,whose effect depends on the dose of LPS. This research suggested that the practical future in which special cytokine target treatment will be adopted on perennial flour mite allergic rhinitis patients.