Search Results

1.First Case Report of Bacteremia Due to Catabacter hongkongensis in a Korean Patient.

Yong Jun CHOI ; Eun Jeong WON ; Soo Hyun KIM ; Myung Geun SHIN ; Jong Hee SHIN ; Soon Pal SUH

Annals of Laboratory Medicine 2017;37(1):84-87

2.Analysis of the Vaginal Microbiome by Next-Generation Sequencing and Evaluation of its Performance as a Clinical Diagnostic Tool in Vaginitis.

Ki Ho HONG ; Sung Kuk HONG ; Sung Im CHO ; Eunkyung RA ; Kyung Hee HAN ; Soon Beom KANG ; Eui Chong KIM ; Sung Sup PARK ; Moon Woo SEONG

Annals of Laboratory Medicine 2016;36(5):441-449

BACKGROUND: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that included bacteria and other microorganisms. The NGS results were compared with the results of other assays, and NGS was evaluated for its feasibility for predicting vaginitis. METHODS: In total, 89 vaginal swab specimens were collected. Microscopic examinations of Gram staining and microbiological cultures were conducted on 67 specimens. NGS was performed with GS junior system on all of the vaginal specimens for the 16S rRNA, internal transcribed spacer (ITS), and Tvk genes to detect bacteria, fungi, and Trichomonas vaginalis. In addition, DNA probe assays of the Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis were performed. Various predictors of diversity that were obtained from the NGS data were analyzed to predict vaginitis. RESULTS: ITS sequences were obtained in most of the specimens (56.2%). The compositions of the intermediate and vaginitis Nugent score groups were similar to each other but differed from the composition of the normal score group. The fraction of the Lactobacillus spp. showed the highest area under the curve value (0.8559) in ROC curve analysis. The NGS and DNA probe assay results showed good agreement (range, 86.2-89.7%). CONCLUSIONS: Fungi as well as bacteria should be considered for the investigation of vaginal microbiome. The intermediate and vaginitis Nugent score groups were indistinguishable in NGS. NGS is a promising diagnostic tool of the vaginal microbiome and vaginitis, although some problems need to be resolved.
Area Under Curve ; Bacteria/*genetics/isolation & purification ; Bacterial Proteins/genetics ; Candida/*genetics/isolation & purification ; Female ; Fungal Proteins/genetics ; Gardnerella vaginalis/genetics/isolation & purification ; High-Throughput Nucleotide Sequencing ; Humans ; *Microbiota ; RNA, Ribosomal, 16S/chemistry/genetics/metabolism ; ROC Curve ; Sequence Analysis, DNA ; Trichomonas vaginalis/genetics/isolation & purification ; Vagina/*microbiology ; Vaginitis/*diagnosis/microbiology

3.First Case Report of Human Infection With Ochrobactrum tritici Causing Bacteremia and Cholecystitis.

Duck Jin HONG ; Keon Han KIM ; Jung Ok KIM ; Jun Sung HONG ; Seok Hoon JEONG ; Kyungwon LEE

Annals of Laboratory Medicine 2016;36(3):278-280

4.Fusobacterium Isolates Recovered From Colonic Biopsies of Inflammatory Bowel Disease Patients in Korea.

Yangsoon LEE ; Chang Soo EUN ; A Reum LEE ; Chan Hyuk PARK ; Dong Soo HAN

Annals of Laboratory Medicine 2016;36(4):387-389

5.Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea.

Bongyoung KIM ; Hyunjoo PAI ; Kwang hyun LEE ; Yangsoon LEE

Annals of Laboratory Medicine 2016;36(6):617-619

6.Flavobacterium ceti From Blood Samples of a Korean Patient With Alcoholic Liver Cirrhosis.

Ji Yeon SUNG ; Taek Soo KIM ; Sue SHIN ; Eun Youn ROH ; Jong Hyun YOON ; Eui Chong KIM

Annals of Laboratory Medicine 2015;35(3):384-386

7.First Case of Pulmonary Mycobacterium parascrofulaceum Infection in a Patient With Bronchiectasis in Korea.

Kyoung Bo KIM ; Sung Gyun PARK ; Jae Seok PARK ; Wonmok LEE ; Jung Sook HA ; Nam Hee RYOO ; Dong Seok JEON ; Jae Ryong KIM

Annals of Laboratory Medicine 2015;35(3):379-381

8.Campylobacter hyointestinalis Isolated From a Human Stool Specimen.

Do Kyun KIM ; Sung Kuk HONG ; Myungsook KIM ; Jin Young AHN ; Dongeun YONG ; Kyungwon LEE

Annals of Laboratory Medicine 2015;35(6):657-659

9.The First Case of Concurrent Infective Endocarditis and Spondylitis Caused by Streptococcus tigurinus.

Borahm KIM ; Hee Jae HUH ; Doo Ryeon CHUNG ; Wook Sung KIM ; Chang Seok KI ; Nam Yong LEE

Annals of Laboratory Medicine 2015;35(6):654-656

10.First Korean Case of Cedecea lapagei Pneumonia in a Patient With Chronic Obstructive Pulmonary Disease.

Sung Kuk HONG ; Jee Soo LEE ; Eui Chong KIM

Annals of Laboratory Medicine 2015;35(2):266-268