Objective:Activating platelets are associated with tumor development and progression.Platelet distribution width(PDW) is an early index of activated platelets.The goal of this study was to investigate the association between prognosis and preoperative PDW in gastric cancer.Methods:A total of 126 gastric cancer patients were retrospectively analyzed from January 2010 to January 2012.The optimal cutoff value of PDW was determined by time-dependent receiver-operating curves.The patients were divided into low and high PDW groups.The prognostic analysis was carried out with univariate analysis and the Cox regression model,which evalu-ated the association of PDW,with progression-free survival(PFS)and overall survival(OS).Results:PDW was closely related with differ-entiation and platelet count.Moreover,lower PDW was associated with decreased PFS and OS in gastric cancer.Multivariate analysis showed that patients with higher PDW had a lower risk of disease progression(hazard ratio:0.562,95% confidence interval:0.184-0.926,P=0.012)and death(hazard ratio:0.468,95% confidence interval:0.263-0.834,P=0.010).Conclusions:Lower preoperative PDW is a poor prognostic factor in gastric cancer,which may also be a prognostic marker in resectable gastric cancer.

Objective To determine the population structure of Streptococcus pncumoniae isolates collected from infants with eye infections,including drug resistance,resistance genes, serotypes and molecular types.Methods The susceptibility of 39 isolates to 10 antibacterial agents was tested by K-B disk diffusion and Etest.Latex agglutination test was performed to determine the serotype of the strains,and PCR was carried out to detect macrolides resistance genes mefE and ermB.Molecular types of the 20 strains were determined by multilocus sequence typing (MLST).Results A total of 39 Streptococcus pneumoniae isolates were obtained,in which 30 (76.9％) were resistant to 3 or more antibacterial agents,and no vancomycin,penicillin or cefotaxime resistant strain was found.ermB gene was found in 33 strains and mefE gene was found in 4 strains.Twelve serotypes were found,and the most frequent serotypes were 19 (8/39) and 14 (4/39). Seventeen strains (43.6％) were covered in PCV7 vaccine. The international clone Taiwan19F-14 and Spain23F-1 were found by MLST. Conclusions Streptococcus pneumoniae isolates from infants with eye infections include international resistance clones.The distribution of serotype and molecular type are dispersed, and the clones are sporadic. The isolates are highly resistant to commonly used antibacterial agents.

OBJECTIVE To understand the urinary tract infections after cesarean section,the distribution of pathogenic strains,and drug resistance analysis in order to provide clinical treatment. METHODS The routine urine cultures of the 2652 case were identified and drug resistance was analyzed by K-B method. RESULTS From 458 isolated bacteria,Gram-negative bacilli were main,pathogen accounting for 80.13%,Escherichia coli and Klebsiella,accounted for 71.62% and 4.8% respectively;Gram-positive bacteria accounted for 14.41%,and fungi accounted for 3.71%. CONCLUSIONS G-bacteria in the urinary tract infection are in the absolute superiority;rational use of antibiotics to effectively control the urinary tract infections and avoid drug-resistant strains produceing is especially critical.

OBJECTIVE To understand the pathogen distribution of infant infectious bacterial conjunctivitis and keratitis,and drug resistance to provide guidence on clinical treatment.METHODS The routine bacterial culture of eye secretions in the 331 cases was identified with K-B to determine the resistance of pathogenic bacteria.RESULTS A total of 124 strains were isolated with positive rate of 37.46%,there were Haemophilus parainfluenzae,Staphylococcus aureus,H.influenzae,S.epidermidis,fungi,etc.The resistance of S.aureus,S.epidermidis and S.pneumoniae to penicillin were 86.36%,88.89% and 0,respectively.The resistance rate of H.parainfluenzae,H.influenzae,Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa to ampicillin were 12.50%,27.78%,87.50%,100.00% and 100.00%;that to cefazolin were 16.67%,16.67%,62.50%,14.29% and 100.00%;and to cotrimoxazole were 50.00%,27.78%,12.50%,14.29% and 100.00%.CONCLUSIONS The H.parainfluenzae,S.aureus,H.influenzaeand S.epidermidis are isolated from the newborn eye secretions;they were lower resistant to ofloxacin and ciprofloxacin.

OBJECTIVE To understand the distribution of pathogen in very-low-birth-weight or preterm infants septicemia in early-onset and late-onset and drug sensitivity.METHODS Bacterial isolates from inpatients of very-low-birth-weight or preterm infants septicemia over a period of 3 years were retrospectively analyzed,and classified as when septicemia was happened.RESULTS The positive rate of isolates was 43.2% in 970 samples of very-low-birth-weight or preterm infants septicemia.Most of the sepsis detected by blood culture was late-onset neonatal sepsis(58.6%).Pathogen of late-onset neonatal sepsis mostly was Gram-positive cocci,Staphylococcus were found to be the most common isolates(80.5%).In early-onset sepsis group,the isolates rates of Gram-negative and Gram-positive bacteria were mostly in proportion,there were 52.4% and 47.6%,respeitively.The resistance rate of late-onset sepsis group was higher than that of early-onset one.CONCLUSIONS The key of curing infants septicemia is that we should master the distribution of pathogen of very-low-birth-weight or preterm infants septicemia in early-onset and late-onset and drug sensitivity.Antimicrobial therapy should be initiated under the guidance of anti-microbial sensitivity test,in order to avoid abuse of antimicrobial.

OBJECTIVE To investigate the distribution and drug resistance of pathogenic bacteria which caused respiratory infection among suscepted patients and offer scientific basis for reasonable usage of antibiotics.METHODS Oropharyngeal swabs among 709 cases of respiratory infection neonates were investigated by the routine methods and drug resistance was analyzed by K-B method.RESULTS Totally 438 bacterial strains were isolated from 709 neonates.most of these bacteria were Gram-negative bacilli(70.3%),among which Haemophilus influenzae and Klebsiella pneumoniae were accounted for 39.8% and 10.3%,respectively;fungi and Gram-positive cocci were accounted for 23.5% and 6.2%.CONCLUSIONS Most strains present higher resistance rates to penicillin and ampicillin;but cefoxitin,amikacin,vancomycin,imipenem and the third generation cephalosporins are revealed with higher sensitivity rates for pathogenic bacteria in newborns.