OBJECTIVE: This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633. METHODS: Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay. RESULTS: The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter-proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism. CONCLUSION One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP.
Bacterial Proteins/metabolism* ; Biofilms ; Cyclic GMP/analogs & derivatives* ; Gene Expression Regulation, Bacterial ; Gentian Violet ; Histones/metabolism* ; Vibrio parahaemolyticus/genetics*

Objective:To analyze the trend and group characteristics of blood lipid metabolism indexes of a batch of military medical staff in a hospital and the interactions among those indexes and other related metabolism indexes，so as to provide reference for systematic health management of blood lipid for military medical personnel.Methods:A total of 236 records of blood lipid metabolism indexes of naval medical personnel who consecutively received physical examinations from 2012 to 2018（except 2013）were collected and analyzed by SPSS 21.0 software.Results:Different gender groups had different trends in blood lipid metabolism. Among male military medical staff，the total cholesterol（TC）in 2015 reached 40.00%；the abnormal rate of high-density lipoprotein cholesterol（HDL-C）rose from 16.49% in 2012 to 22.22% in 2018；and the abnormal rate of low-density lipoprotein cholesterol（LDL-C）rose from 43.30% in 2012 to 58.89% in 2018. Among female military medical staff，the abnormal rates of blood lipid levels increased year by year，in 2018，the abnormal rates of TC，triacylglycerol（TG），and LDL-C were 18.89%，5.56%，and 30.00%，respectively. The abnormal rates of blood lipid levels in female military medical personnel were significantly lower than those of their male counterparts. Different age groups had different lipid metabolism characteristics. The military medical personnel aged under 40（group < 40）had relatively high TC in 2014，2015，and 2017. Their TG abnormal rate in 2015 was at the peak value of 56.36%；the highest abnormal rate of their HDL-C was 21.43% in 2018；the highest abnormal rate of their LDL-C was 68.57% in 2018. The military medical personnel aged 40–50（group 40–50）in 2018 had a TC abnormal rate of 36.59%. Their LDL-C abnormal rate of 75.61%，and their abnormal rate of HDL-C were declining. The military medical personnel aged above 50（group > 50）showed an increasing trend in abnormal rates of TC，TG，and LDL-C，reaching 43.75%，37.5%，and 68.75%，respectively，in 2018. In 2012，the abnormal rates of blood lipid metabolism indexes in the group > 50 was lower than those of the other two groups，while in 2018，the abnormal rate of TG in the group > 50 was significantly higher than those of the other two groups，and the abnormal rate of HDL-C in the group < 40 was significantly higher than those of the other two groups. Comparing the mean values of blood lipid metabolism indexes of different age groups in different years，the mean values of TC in the group 40–50 and the group > 50 fluctuated significantly，and there were two high mean values of TC in 2015 and 2017. The mean value of HDL-C in 2012 was significantly lower than those in other years（ F=6.312， P<0.001）. Comparing the mean values of blood lipid metabolism indexes between different age groups in the same year，the mean values of TC in 2012 and 2015 of different age groups were significantly different，among which the mean value of TC in the group > 50 group was significantly higher than that of the group < 40（ F=3.597， P=0.026； F=4.253， P=0.007）；the mean value of LDL-C of the group > 50 in 2012 was significantly higher than that of the group < 40 in 2012（ F=8.660， P<0.001）. The cumulative changes of fasting blood glucose and serum uric acid were positively correlated with the cumulative changes of TC and TG. Conclusion:The blood lipid metabolism of military medical personnel has significant group characteristics，so it is necessary to provide targeted long-term health support programs according to such characteristics，so as to maintain the long-term stability of blood lipid metabolism in different groups.

Objective:To analyze the trend and group characteristics of blood lipid metabolism indexes of a batch of military medical staff in a hospital and the interactions among those indexes and other related metabolism indexes，so as to provide reference for systematic health management of blood lipid for military medical personnel.Methods:A total of 236 records of blood lipid metabolism indexes of naval medical personnel who consecutively received physical examinations from 2012 to 2018（except 2013）were collected and analyzed by SPSS 21.0 software.Results:Different gender groups had different trends in blood lipid metabolism. Among male military medical staff，the total cholesterol（TC）in 2015 reached 40.00%；the abnormal rate of high-density lipoprotein cholesterol（HDL-C）rose from 16.49% in 2012 to 22.22% in 2018；and the abnormal rate of low-density lipoprotein cholesterol（LDL-C）rose from 43.30% in 2012 to 58.89% in 2018. Among female military medical staff，the abnormal rates of blood lipid levels increased year by year，in 2018，the abnormal rates of TC，triacylglycerol（TG），and LDL-C were 18.89%，5.56%，and 30.00%，respectively. The abnormal rates of blood lipid levels in female military medical personnel were significantly lower than those of their male counterparts. Different age groups had different lipid metabolism characteristics. The military medical personnel aged under 40（group < 40）had relatively high TC in 2014，2015，and 2017. Their TG abnormal rate in 2015 was at the peak value of 56.36%；the highest abnormal rate of their HDL-C was 21.43% in 2018；the highest abnormal rate of their LDL-C was 68.57% in 2018. The military medical personnel aged 40–50（group 40–50）in 2018 had a TC abnormal rate of 36.59%. Their LDL-C abnormal rate of 75.61%，and their abnormal rate of HDL-C were declining. The military medical personnel aged above 50（group > 50）showed an increasing trend in abnormal rates of TC，TG，and LDL-C，reaching 43.75%，37.5%，and 68.75%，respectively，in 2018. In 2012，the abnormal rates of blood lipid metabolism indexes in the group > 50 was lower than those of the other two groups，while in 2018，the abnormal rate of TG in the group > 50 was significantly higher than those of the other two groups，and the abnormal rate of HDL-C in the group < 40 was significantly higher than those of the other two groups. Comparing the mean values of blood lipid metabolism indexes of different age groups in different years，the mean values of TC in the group 40–50 and the group > 50 fluctuated significantly，and there were two high mean values of TC in 2015 and 2017. The mean value of HDL-C in 2012 was significantly lower than those in other years（ F=6.312， P<0.001）. Comparing the mean values of blood lipid metabolism indexes between different age groups in the same year，the mean values of TC in 2012 and 2015 of different age groups were significantly different，among which the mean value of TC in the group > 50 group was significantly higher than that of the group < 40（ F=3.597， P=0.026； F=4.253， P=0.007）；the mean value of LDL-C of the group > 50 in 2012 was significantly higher than that of the group < 40 in 2012（ F=8.660， P<0.001）. The cumulative changes of fasting blood glucose and serum uric acid were positively correlated with the cumulative changes of TC and TG. Conclusion:The blood lipid metabolism of military medical personnel has significant group characteristics，so it is necessary to provide targeted long-term health support programs according to such characteristics，so as to maintain the long-term stability of blood lipid metabolism in different groups.

The poor stability of the ligustilide (LIG) makes its quantitation in Angelica sinensis (AS) difficult. This study establishes a chemical conversion method for the determination of ligustilide content in AS and proposes a national pharmacopoeia standard. Mechanical agitation and sonication of a powdered AS extract in a methanol/cyprolamine mixture facilitated the stabilization and transformation of ligustilide. Using an external reference HPLC-DAD method, the cyclopropyl-ligustilide (LIGc) content in the mixture could be determined. The content of ligustilide was greater than 1.0% based on 144 AS specimens including 68 obtained from the originally planted areas of Qinghai and Gansu Province; 55 specimens were obtained from Minxian and Weiyuan County medicine markets, and 21 specimens for which the storage period reached or exceeded 1.5 years. According to the Hong Kong Chinese materis medica standards, the content of ligustilide in AS should not be lower than 0.6%. The developed method could also be applied to the quality control of other Chinese medicinal materials (such as Ligusticum chuanxiong) or Chinese patent medicines in which ligustilide is the main component.

Thirteen compounds were isolated and purified from the leaves of Cinnamomum camphora by the macroporous resin,silica gel,and Sephadex LH-20 column chromatographies. Those compounds were further identified by IR,UV,MS,and NMR techniques:( 2 S)-1-( 3″,4″-methylenedioxy phenyl)-3-( 2',6'-dimethoxy-4'-hydroxyphenyl)-propan-2-ol( 1),( 2 R,3 R)-5,7-dimethoxy-3',4'-methylenedioxy flavanol( 2),9-hydroxysesamin( 3),sesamin( 4),piperitol( 5),kobusin( 6),(-)-aptosimon( 7),acuminatolide( 8),1β,11-dihydroxy-5-eudesmene( 9),lasiodiplodin( 10),vanillin( 11),p-hydroxybenzaldehyde( 12),and p-hydroxybenzoic acid ethyl ester( 13). Compound 1 was a novel compound,and compounds 2,6,7,9 and 10 were isolated from Cinnamomum plants for the first time. Compounds 4,7 and 10 were found to possess good inhibitory effect on IL-6 production in LPS-induced BV2 cells at a concentration of 20 μmol·L-1 in the in vitro bioassay,with inhibition rates of 51. 26% ± 4. 13%,67. 82% ± 3. 77% and85. 81%±1. 19%,respectively.
Anti-Inflammatory Agents/pharmacology* ; Cinnamomum ; Cinnamomum camphora ; Plant Leaves

OBJECTIVE: To explore the distribution characteristics of main antigen gene frequencies of Duffy,Diego,Kidd,Dombrock,MNS,Lutheran,Kell,Colton,Scianna,Yt,Knops and Indian in red blood cell blood group system of Li nationality in Hainan Province. METHODS: Antigens in twelve rare blood group systems of 214 Li people in Hainan Province were genotyped and analyzed by polymerase chain reaction-sequence specific primers (PCR-SSP). RESULTS: The gene frequency of antigens in twelve rare blood group systems of 214 Li people in Hainan Province including: the gene frequency of Duffy blood group system: fy CONCLUSION The genetic distribution and genetic status in twelve rare blood group systems of Li nationality in Hainan Province are relatively stable. The gene distribution of Duffy, Diego, Kidd, Drombrock, MNS and Lutheran blood group systems are polymorphic and show unique distribution characteristics compared with other regions and different nationalities. The gene frequency distribution of Kell、Colton、Scianna、Yt、Knops、Indian blood group systems are monomorphic.
Blood Group Antigens/genetics* ; Ethnic Groups ; Gene Frequency ; Genotype ; Humans ; Kidd Blood-Group System ; Polymorphism, Genetic

OBJECTIVE Only limited number of drugs are currently available for treating ischemic stroke. Therapeu?tic angiogenesis has recently emerged as one of the most promising therapies for cerebral ischemic injury. Isopropyl-β-(3,4-dihydroxyphenyl)-α-hydroxypropanoate (IDHP) is a metabolite derived from the botanical formulation for Dantonic?. Here, we investigated the angiogenic efficacy of IDHP in cerebral ischemia. METHODS The in vivo effects of IDHP were evaluated in the C57BL/6 mouse Matrigel plug and rat transient middle cerebral artery occlusion (tMCAO) models. Primary human umbilical vein endothelial cells (HUVEC) and human brain microvascular endothelial cells (HBMEC) were used to explore the effects of IDHP on stimulating proliferation, migration and tube formation in vitro. ELISA and Western blotting were used to quantitate the release and expression of relevant target molecules and signaling path?ways. RESULTS IDHP reduced infarct volume and improved sensorimotor function in rats subjected to tMCAO by pro?moting angiogenesis, and promoted Matrigel neovascularization in mice. Moreover, IDHP produced a biphasic modula?tion on proliferation and migration both in HUVEC and HBMEC. It also induced tube formation in a 12-day HUVEC-HDF co-culture model and in Matrigel assays. IDHP-induced angiogenesis was accompanied by increased levels of p-AMPKα (Thr172) and p-eNOS (Ser1177) both in vitro and in vivo, and the decreased level of VEGF in rat brains on day 1 whereas enhanced level of VEGF on day 3 and 7 after tMCAO. Mechanistically, AMPK knockdown or pharmacologi?cally inhibiting AMPK and its upstream kinases (CaMKKβ) inhibited the eNOS phosphorylation induced by IDHP in HUVEC. Furthermore, selective eNOS inhibitor (L-NIO), selective CaMKKβ inhibitor (STO) and AMPKa inhibitor (Com?pound C) blocked the capillary-like tube formation in the co-culture model induced by IDHP (10 nmol · L-1). CONCLU?SION Collectively, these findings showed that IDHP protected rats from cerebral ischemia-reperfusion injury by promot?ing angiogenesis via activating CaMKKβ/AMPK(Thr172)/eNOS(Ser1177) signaling, and suggest it to be a promising new drug candidate for the prevention and/or treatment of cerebral ischemia and other vascular occlusive diseases.

Aim To investigate the protective effect of cyclovirobuxine D(CVB-D) on aldosterone (ALD)-induced primary neonatal rat cardiac myocytes (PNRC-Ms) injury and the possible mechanism. Methods PNRCMs were extracted by trypsin, and the PNRCMs injury model was established by ALD (10 μmol · L

ObjectiveThere are few reports about abnormal oligonucleotide binding fold domain protein genes (OBGs) affecting the initiation of DNA replication in hepatocellular carcinoma through the microchromosome maintenance (MCM) complex. This study aims to explore the roles of reverse-transcription-related genes (RTGs) in Hepatocellular Carcinoma cells (HCC) and the correlation between gene polymorphisms and abnormal gene expression.Methods We created a mouse model by injecting hepatocellular carcinoma cell line H22 (logarithmic growth phase) and dissected the tumor bodies from tumor-forming mice. The control group was treated by isotonic saline without H22. The healthy liver tissue cells were taken from the control mice. The total RNA of the H22 group and control group were extracted, and differentially expressed genes were analyzed. Screening of differentially expressed reverse transcription-related DEGs (RDEGs), GO and KEGG analysis of RDEGs. The interaction analysis of RDEGs encoded proteins, and the correlation analysis of RDEGs polymorphism and gene expression.ResultsThere were 193 differentially expressed RTGs in HCCs, which were involved in two biological procedures, three cell components, one molecular function, three signal pathways, and three functional sites; Its function is mainly concentrated in DNA replication, especially the construction of MCM complex and telomere complex in which OBGs participate in the initiation of replication. Most related genes had OB fold domains. The results also showed that both AS and SNV caused gene polymorphism was positively correlated with gene expression, and most OBGs in HCC had SNV phenomenon, but not occurred in healthy liver tissue.Conclusion Collectively, AS and SNV may be important regulatory factors for gene expression. SNV may particularly affect the function of OBGs in the MCM complex to abnormally initiate DNA replication in HCC.

Objective This study combined genotyping with family doctors' contracting model to assess the application of precision medicine in rural patients with essential hypertension. Methods In this study, 209 hypertensive patients from 3 villages in Lujiang County, Hefei City, Anhui Province were selected as subjects and randomly divided into experimental group(n=105) and control group(n=104). The medication regimen of observation group was guided by genetic testing for gene sensitivity to antihypertensive drugs, and the control group was implemented routine pharmacy. All the patients were managed by family doctors. Adverse drug reaction rate, treatment compliance, blood pressure, body mass index (BMI), fasting blood glucose (FBG), cholesterol (TC), and triglycerides (TG) of the two groups were analyzed, respectively, during the 6-month intervention. Results After 6-month of intervention, the medication compliance of the experimental group were significantly higher than that of the control group, and the blood pressure and adverse drug reaction rate were significantly lower than that of the control group. After 3 months of intervention, there was no significant decrease in BMI, FBG, TC and TG in the two groups. After 6 months of intervention, the FBG, TC and TG of the experimental group were significantly decreased,while only the FBG value of the control group was significantly decreased. There were no significant changes in body mass index (BMI) values in both groups. Conclusions Individualized medication guided by genotyping can improve the treatment compliance, reduce the adverse drug reaction rate, and improve the treatment efficiency of patients with essential hypertension.