Objective To explore the effect and mechanism of ITPKB on testosterone synthesis disorder of senescent Leydig cells.Methods A comprehensive analysis of public datasets was performed using GO,GSEA enrichment analysis and protein interaction network to determine the expression of related pathways and genes in testicular tissue in aging-related diseases.D-galactose intraperitoneal injection to construct an aging mouse model;Mouse Leydig cells(TM3)were cultured,and the cells were divided into four groups,control group(NC group),senescence group(D-gal group),inhibitor group(GNF362 group)and D-gal+GNF362 group.The serum levels of testosterone,follicle-stimulating hormone and luteinizing hormone in mice were detected by ELISA.q-PCR was used to detect the mRNA levels of IL-1α,IL-6,TNF-α and ITPKB.Immunofluorescence was used to detect the protein expressions of StAR,3β-HSD,ITPKB and LC3.Western blot was used to detect the protein expressions of P53,P21,StAR and ITPKB.Transmission electron microscopy to observe intracellular structures.Results Bioin-formatics analysis showed that ITPKB was highly expressed in the testes of aging mice.The serum testosterone level of aging mice was lower than that of young mice.Compared with the younger group,the mRNA levels of IL-1α,IL-6,TNF-α and ITPKB in the testes of mice in the aging group were increased,and the protein levels of P53,P21 and ITPKB were increased.The levels of StAR,3β-HSD,LC3 protein and mitophagy decreased.Compared with the cells in the D-gal group,the testosterone level in the supernatant of the D-gal+GNF362 group was increased,and the level of mitophagy was significantly increased.Conclusion ITPKB causes impaired testosterone synthesis in senescent Leydig cells by inhibiting mitophagy.

Objective To explore the effect and mechanism of ITPKB on testosterone synthesis disorder of senescent Leydig cells.Methods A comprehensive analysis of public datasets was performed using GO,GSEA enrichment analysis and protein interaction network to determine the expression of related pathways and genes in testicular tissue in aging-related diseases.D-galactose intraperitoneal injection to construct an aging mouse model;Mouse Leydig cells(TM3)were cultured,and the cells were divided into four groups,control group(NC group),senescence group(D-gal group),inhibitor group(GNF362 group)and D-gal+GNF362 group.The serum levels of testosterone,follicle-stimulating hormone and luteinizing hormone in mice were detected by ELISA.q-PCR was used to detect the mRNA levels of IL-1α,IL-6,TNF-α and ITPKB.Immunofluorescence was used to detect the protein expressions of StAR,3β-HSD,ITPKB and LC3.Western blot was used to detect the protein expressions of P53,P21,StAR and ITPKB.Transmission electron microscopy to observe intracellular structures.Results Bioin-formatics analysis showed that ITPKB was highly expressed in the testes of aging mice.The serum testosterone level of aging mice was lower than that of young mice.Compared with the younger group,the mRNA levels of IL-1α,IL-6,TNF-α and ITPKB in the testes of mice in the aging group were increased,and the protein levels of P53,P21 and ITPKB were increased.The levels of StAR,3β-HSD,LC3 protein and mitophagy decreased.Compared with the cells in the D-gal group,the testosterone level in the supernatant of the D-gal+GNF362 group was increased,and the level of mitophagy was significantly increased.Conclusion ITPKB causes impaired testosterone synthesis in senescent Leydig cells by inhibiting mitophagy.

OBJECTIVETo investigate the protective effect of bone marrow mesenchymal stem cells (BMSCs)-derived exosomesagainst testicular ischemia-reperfusion injury (IRI) in rats.

METHODSRat BMSCs were isolated, cultured and identified in theprimary culture. The exosomes were extracted from the BMSCs and characterized using nanoparticle tracking analysis, transmission electron microscopy, and Western blotting. Twenty-four healthy male SD rats were randomly divided into shamoperation group, testicular IRI with saline treatment group and IRI with exosome treatment group. The contralateral testes ofthe rats were collected for pathological observation, aseessment of superoxide dismutase (SOD) and malondialdehyde (MDA), and detection of HMGB1, caspases-3 and cleaved caspase-3 expressions using Western blotting.

RESULTSWe successfullyobtained exosomes from rat BMSCs. Testicular IRI significantly impaired testicular spermatogenesis, which was markedlyimproved by treatment with the exosomes ( < 0.05). Testicular IRI also caused significant increase in the protein expression ofHMGB1, caspase-3 and cleaved caspase-3 in the testicular tissue, and treatment with the exosomes obviously amelioratedthese changes ( < 0.05).

CONCLUSIONSBMSCs-derived exosomes protects against testicular IRI due to the anti-oxidant, antiinflammatory and anti-apoptosis activities of the exosomes.

OBJEVTIVETo investigate the expression of cysteine-rich secretory protein 2 (CRISP2) in spermatozoa of patients with asthenozoospermia and explore its clinical significance.

METHOSSemen samples were collected from 24 normal volunteers and 24 patients with asthenozoospermia for detecting CRISP2 mRNA and protein expressions using qRT-PCR and Western blotting, respectively. The correlation of CRISP2 expressions with sperm morphology, progressive motility and fertility prognosis were analyzed in patients with asthenozoospermia.

RESULTSCRISP2 protein expression was obviously lowered in the ejaculated spermatozoa of patients with asthenozoospermia as compared to the normal volunteers, but no significant difference in CRISP2 mRNA expression was found between the two groups. Correlation analysis showed that CRISP2 protein expression was positively correlated with normal sperm morphology (r=0.6182, P=0.0037) and progressive motility (r=0.6309, P=0.0029). Follow-up study of the patients revealed a higher fertility rate in patients with a relatively high CRISP2 protein expression than in those with low CRISP2 protein expression (80.0% vs 20.0%, P=0.0230).

CONCLUSIONThe expression level of CRISP2 protein is positively correlated with normal sperm morphology and progressive motility. A reduced CRISP2 protein expression indicates poor fertility prognosis of patients with asthenozoospermia, suggesting the potential value of CRISP2 as a novel therapeutic target for treating asthenozoospermia.

Asthenozoospermia ; metabolism ; Case-Control Studies ; Fertility ; Follow-Up Studies ; Glycoproteins ; metabolism ; Humans ; Male ; RNA, Messenger ; Sperm Motility ; Spermatozoa ; metabolism

OBJECTIVETo evaluate the effect of placement of urethral catheter combined with lidocaine on urethral irritation caused by postoperative indwelling catheters.

METHODSA total of 120 male surgical patients requiring postoperative indwelling catheters between June 2011 and January 2012 were divided into two equal groups for placement of painless urethral catheter combined with bladder washing with lidocaine on the first postoperative day, or for routine catheter placement only. The symptoms of urethral irritation such as urethral pain, urinary urgency, and perineal discomforts were observed and compared between the two groups.

RESULTSIn patients with painless urethral catheter placement combined with bladder washing with lidocaine, 11 developed urethral irritation symptoms, as compared to 24 in the patients with routine catheter placement only, showing a significant difference between the two groups (P<0.05).

CONCLUSIONPlacement of painless urethral catheter combined with bladder washing with lidocaine can significantly reduce the incidence of urethral irritation due to postoperative indwelling catheters.

Adult ; Humans ; Lidocaine ; Male ; Middle Aged ; Urinary Catheterization ; methods ; Urinary Catheters ; adverse effects ; Urination Disorders ; etiology ; prevention & control ; Young Adult

OBJEVTIVETo analyze the complications of extracorporeal shock wave lithotripsy (ESWL) for treatment of lower urinary tract stones and identify the factors contributing to the complications.

METHODSWe retrospectively analyzed the postoperative complications of ESWL in 83 patients with lower urinary tract stones and their association with the patients' age, height, body weight, body mass index (BMI), stone features, shock wave times, and shock wave energy.

RESULTSOf the 83 cases treated with ESWL, 72.3% (60 cases) showed gross hematuria, 27.7% (23 cases) complained of pain, and 25.3% (21 cases) had both pain and gross hematuria. None of the patients reported vomiting, skin rash, flatulence, gastrointestinal bleeding or other complications. Patients with postoperative gross hematuria, pain, and both had significantly greater stone length, stone width and more shock body times than those without these complications (P<0.01 or P<0.05). Patients with gross hematuria had significantly lower height than those without complications (P<0.05).

CONCLUSIONThe common complications of ESWL for lower urinary tract stones include hematuria and pain. The patient's height, stone length, stone width and shock times are important factors contributing to these postoperative complications.

Abdominal Pain ; etiology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Hematuria ; etiology ; Humans ; Lithotripsy ; adverse effects ; Male ; Middle Aged ; Retrospective Studies ; Urinary Bladder Calculi ; therapy ; Young Adult