Objective The morphological and data characteristics of premature ventricular contraction(PVC)were obtained through dynamic electrocardiogram(DCG),and its correlation with new-onset major adverse cardiovascular events(MACE)was analyzed.Methods A total of 115 patients with PVC admitted to Huzhou First People's Hospital from January 2022 to January 2024 were retrospectively selected and divided into MACE group(n=31)and non-MACE group(n=84)according to the new-onset MACE.Collect the baseline data of patients,as well as DCG data such as the morphological and data characteristics of PVC.Collect the baseline data of patients,as well as DCG data such as the morphological and data characteristics of PVC.Multivariate Logistic regression was used to analyze the influencing factors of new-onset MACE in patients with PVC.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of influencing factors for the risk of new-onset MACE in patients with PVC.Results There were statistically significant differences in gender,disease duration,QRS wave duration,PVC origin,PVC load,and Lown classification between two groups of patients(P＜0.05).Multivariate Logistic regression analysis showed that QRS wave duration(OR=1.070),PVC origin(OR=6.840)and PVC load(OR=2.583)were independent risk factors for new-onset MACE in PVC patients(P＜0.05).The ROC curve showed that the area under the curve for predicting new-onset MACE in PVC patients with QRS wave duration,PVC origin,and PVC load were 0.791,0.750,and 0.917 respectively.The predictive value of PVC load was the highest.Conclusion QRS wave duration,PVC origin and PVC load are related to new-onset MACE in PVC patients,and PVC load has a good predictive effect on the risk of new-onset MACE in PVC patients.

Objective To explore the value of ventricular response(VR)on ambulatory electrocardio-graphy in evaluating atrial fibrillation conversion in patients with paroxysmal atrial fibrillation(PAF).Meth-ods Used 3-lead Holter monitor to collect complete data for more than 3 hours before atrial fibrillation con-version.The irregular heart rates recorded 5 minutes(VR0-5 min),60-65 minutes(VR60-65 min),and 120-125 minutes(VR120-125 min)prior to atrial fibrillation conversion were designated as VR values.The average heart rates of the 10 consecutive fastest and slowest heartbeats were calculated as the maximum and minimum VR values,respectively,while the average heart rate during the entire atrial fibrillation episode was taken as the mean VR value.Atrial fibrillation conversions occurring 06:00-21:59 were classified as daytime events,and those occurring 22:00-05:59(the following day)were classified as nighttime events.The effects of gender differences and circadian rhythm variations on VR values before atrial fibrillation conversion were analyzed.Additionally,the low-frequency power(LF),high-frequency power(HF),and their ratio(LF/HF)were ob-tained 5 minutes,60-65 minutes,and 120-125 minutes before atrial fibrillation conversion,and differences in LF,HF,and LF/HF at these time points were analyzed.Results The minimum VR0-5 min before cardioversion of atrial fibrillation was significantly higher than the minimum VR60-65 min and the minimum VR120-125 min(P＜0.05).The maximum VR0-5 min was significantly higher than VR120-125 min(P＜0.05).The minimum VR0-5 min in male group and female group was significantly higher than the minimum VR60-65 min and the minimum VR120-125min before atrial fibrillation reverted(P＜0.05).Of the 86 patients in this study,51 patients(59.30％)experienced daytime events,and the remaining 35 patients(40.70％)experienced nighttime e-vents.The data showed that the minimum VR0-5 min of daytime events was significantly higher than the mini-mum VR60-65 min and the minimum VR120-125 min(P＜0.05).There was no significant difference between the minimum VR0-5 min and the minimum VR60-s5 min and the minimum VR120-125 min(P＞0.05).One-way repeated analysis of variance showed that the longitudinal differences of HF,LF and LF/HF ratio before cardioversion of atrial fibrillation were not statistically significant(P＞0.05).Conclusion The increase of VR value may predict imminent cardioversion in PAF patients,particularly for those experiencing daytime events.

Objective The morphological and data characteristics of premature ventricular contraction(PVC)were obtained through dynamic electrocardiogram(DCG),and its correlation with new-onset major adverse cardiovascular events(MACE)was analyzed.Methods A total of 115 patients with PVC admitted to Huzhou First People's Hospital from January 2022 to January 2024 were retrospectively selected and divided into MACE group(n=31)and non-MACE group(n=84)according to the new-onset MACE.Collect the baseline data of patients,as well as DCG data such as the morphological and data characteristics of PVC.Collect the baseline data of patients,as well as DCG data such as the morphological and data characteristics of PVC.Multivariate Logistic regression was used to analyze the influencing factors of new-onset MACE in patients with PVC.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of influencing factors for the risk of new-onset MACE in patients with PVC.Results There were statistically significant differences in gender,disease duration,QRS wave duration,PVC origin,PVC load,and Lown classification between two groups of patients(P＜0.05).Multivariate Logistic regression analysis showed that QRS wave duration(OR=1.070),PVC origin(OR=6.840)and PVC load(OR=2.583)were independent risk factors for new-onset MACE in PVC patients(P＜0.05).The ROC curve showed that the area under the curve for predicting new-onset MACE in PVC patients with QRS wave duration,PVC origin,and PVC load were 0.791,0.750,and 0.917 respectively.The predictive value of PVC load was the highest.Conclusion QRS wave duration,PVC origin and PVC load are related to new-onset MACE in PVC patients,and PVC load has a good predictive effect on the risk of new-onset MACE in PVC patients.

Objective To investigate the role and mechanism of the DNA helicase PIF1 in the proliferation of ovarian cancer cells and its response to DNA damage.Methods The relative expression of the PIF1 gene in normal ovarian tissue compared to ovarian cancer tissue,as well as the relationship between PIF1 expression and overall survival in ovarian cancer patients,was analyzed using the Gene Expression Profiling Interactive Analysis(GEPIA)and Kaplan-Meier Plotter public databases.PIF1 knockout ovarian cancer cells were established using CRISPR/Cas9 gene-editing technology.A retroviral vector overexpressing Rad51 recombinase was constructed,and then transfected into PIF1 knockout ES-2 ovarian cancer cells.Western blot analysis was used to determine the effects of PIF1 knockout and Rad51 overexpression in the transfected cells.Cell proliferation was assessed with cell counting,colony formation assay and CCK-8 assay.A total of 32 female BALB/c nude mice(6～8 weeks old,weighing 20～25 g)were randomly divided into ES-2 control group,ES-2 knockout group,OVCAR-3 control group,and OVCAR-3 knockout group,with 8 mice in each group.A mouse xenograft model was established to assess the in vivo proliferative capacity of ovarian cancer cells.Apoptotic rate and cell cycle were assessed using flow cytometry.The senescence of ovarian cancer cells was evaluated through a β-galactosidase activity assay.Western blotting and immunofluorescence assay were applied to determine the changes in phosphorylated histone H2AX(γ-H2AX)protein were measured with to evaluate the effects of PIF1 knockout and Rad51 overexpression on DNA damage and to observe the localization of PIF1 and Rad51 in ovarian cancer cells.Results The analysis of public databases revealed that PIF1 overexpression was negatively correlated with the overall survival of patients(P＜0.001),and PIF1 was found to be overexpressed in the ovarian cancer tissues than the normal ovarian tissues(P＜0.05).CRISPR/Cas9-mediated knockout of PIF1 significantly inhibited the proliferation(P＜0.01)and clonogenic ability(P＜0.001)of ovarian cancer cells,which was also validated in mouse model(P＜0.05).Flow cytometry indicated that PIF1 knockout promoted apoptosis(P＜0.01)and induced cell cycle arrest(P＜0.01)in ovarian cancer cells.In addition,β-galactosidase activity assay demonstrated that PIF1 knockout enhanced cellular senescence(P＜0.001).Western blot and CCK-8 assays further revealed that PIF1 knockout increased the expression of γ-H2AX protein(P＜0.05)and suppressed the proliferative capacity of ovarian cancer cells following cisplatin treatment(P＜0.05).In PIF1 knockout ovarian cancer cells,Rad51 expression was diminished.However,overexpression of Rad51 in PIF1-deficient cells restored PIF1 expression,decreased γ-H2AX protein level(P＜0.05),and rescued cell proliferation(P＜0.01).Immunofluorescence assay demonstrated that EGFP-PIF1 and Rad51 were co-localized in the nucleus.Conclusion PIF1 and Rad51 collaboratively regulate DNA damage and cell proliferation in ovarian cancer cells.

Objective:To investigate the influential factors for poor prognosis in patients with acute myocardial infarction and construct a risk prediction nomograph model.Methods:A total of 173 patients with acute myocardial infarction who received treatment in The First People's Hospital of Huzhou from June 2018 to June 2021 were included in this study. They were divided into a good prognosis group ( n = 130) and a poor prognosis group ( n = 43) according to the follow-up results at 6 months after developing acute myocardial infarction. The clinical data of the two groups were compared using retrospective analysis methods. The potential influential factors were preliminarily screened using LASSO regression analysis. The influential factors of poor prognosis for acute myocardial infarction were investigated using logistic regression analysis. The risk prediction nomograph model was constructed using the "rms" package of R 4.2.6 language. The discriminability, calibration, and effectiveness of the model were evaluated by drawing the receiver operating characteristic curve, calibration curve, and decision curve. Model validation was conducted internally using the Bootstrap method (repeated sampling 1 000 times). Results:There were significant differences in the culprit vessel, Killip classification, vessel opening time, cardiac troponin I (cTnI), hypertension history, N-terminal pro-brain natriuretic peptide (NT-proBNP), diabetes history, creatinine, hyperlipidemia history, left ventricular ejection fraction, smoking history and creatine kinase isoenzymes-MB between the two groups (all P < 0.05). Seven potential influential factors were screened using LASSO regression model, including diabetes history, infarcted vessel anterior descending branch, Killip IV, vascular opening time, cTnI, NT-proBNP, and left ventricular ejection fraction. Logistic regression analysis showed that vascular opening time ( OR = 0.171, 95% CI: 0.053-0.548, P = 0.003), cTnI ( OR = 0.201, 95% CI: 0.079-0.510, P = 0.001), left ventricular ejection fraction ( OR = 1.469, 95% CI: 1.167-1.847, P = 0.001), NT-proBNP ( OR = 0.996, 95% CI: 0.993-1.00, P = 0.025) were independent influential factors of poor prognosis in patients with acute myocardial infarction (all P < 0.05). Linear regression analysis results indicate that the regression model did not exhibit significant multicollinearity (variance inflation factor < 10). Based on the four influential factors identified by logistic regression analysis, a nomogram model for predicting the poor prognosis of patients with acute myocardial infarction was developed. The area under the receiver operating characteristic curve was 0.979 [95% CI (0.959, 0.999)], and the consistency index was 0.934. The calibration curve of the model was close to the ideal curve. Decision curve analysis revealed that when the probability threshold predicted by the model ranged from 0.61 to 0.99, the predictive value of the model was superior. Conclusion:Factors influencing the poor prognosis of acute myocardial infarction include the time of vessel opening, cTnI, NT-proBNP, and left ventricular ejection fraction. The constructed nomogram model demonstrates good efficacy in predicting the poor prognosis of patients with acute myocardial infarction and can provide some reference for clinical doctors and nurses to identify patients with poor prognosis as soon as possible.

Objective:To study the effect of liraglutide on the forkhead box O3a (forkhead box O3a, FoxO3a) /Wnt signaling pathway and vertebral bone density in osteoporotic rats.Methods:Female Sprague-dawley rats were divided into sham operation group, model group, and liraglutide intervention group according to the random number table method, with 10 rats in each group. Both the model group and the intervention group used bilateral oophorectomy to establish an osteoporosis model. The intervention group was injected subcutaneously with liraglutide every day, and the sham operation group and the model group were given an equal volume of normal saline. After 12 weeks of continuous administration, the bone mineral density and bone biomechanics were measured, the serum osteoprotegerin, anti-tartrate acid phosphatase, and osteocalcin levels were detected by enzyme-linked immunosorbent assay, and the O3a/Wnt pathway was detected by Real-time PCR technology. The expression level of mRNA was detected by Western blot to detect the expression level of related proteins in the O3a/Wnt pathway.Results:The bone mineral density levels of L4,5 (0.33±0.04 vs 0.18±0.03) and left and right femurs (0.37±0.05 vs 0.23±0.04 0.35±0.04 vs 0.24±0.03) of the successfully modeled rats were significantly lower than those of the sham operation group ( P<0.05) . After 12 weeks of treatment, the differences in the maximum bone load, three-point bending stress, bone density and elastic modulus of the three groups of rats were statistically significant. Among them, the sham operation group had the highest level of each index (36.53±5.23, 154.13±6.27, 0.34±0.04, 3 102.34±160.44) , followed by the intervention group (19.37±4.32, 141.54±6.58, 0.18±0.03, 2 270.18±145.53) and the model group in turn (26.17±4.68, 147.56±5.84, 0.28±0.03, 2 804.24±140.42) ( P<0.05) . There were statistically significant differences in the levels of serum osteoprotegerin, anti-tartrate acid phosphatase and osteocalcin among the three groups. Among them, the osteoprotegerin (Sham operation group vs model group vs intervention group: 7.53±0.63 vs 4.57±0.42 vs 6.15±0.61) of the sham operation group was significantly higher than that of the intervention group and the model group. The anti-tartrate acid phosphatase (Sham operation group vs model group vs intervention group: 14.34±2.87 vs 19.53±3.52 vs 15.96±3.14) and osteocalcin levels (Sham operation group vs model group vs intervention group: 0.84±0.09 vs 1.13± 0.12 vs 0.95± 0.08) of rats The factor was significantly lower than that of the intervention group and model group ( P<0.05) . The mRNA and protein expression levels of FoxO3a, Wnt2, and β-catenin in the three groups of rats were significantly different. Among them, Wnt2 and β-catenin in the sham operation group were significantly higher than the intervention group and model group, and FoxO3a was significantly lower than the intervention group and model Group ( P<0.05) . Conclusion:Liraglutide can increase bone density and improve bone biomechanics by activating O3a/Wnt signal, thereby effectively treating osteoporosis.

Objective To explore the feasibility and effectiveness of aesthetic restoration of caries of mandibular deciduous incisors with strip crown form of maxillary deciduous incisors.Methods A total of 64 patients (202 teeth) with symmetrical caries of bilateral mandibular central incisors or lateral incisors were randomly selected.The opposite maxillary incisors were restored with strip crowns form and the opposite incisors were restored with resin composite.The effects of the two methods and the incidence of complications were compared three months,six months and twelve months after treatment.Results Three months after treatment,there was no significant difference in the success rate between the two groups.Six months after treatment,there was significant differencein the marginal adaptation between the two groups (x2 =3.919,P =0.048);12 months after treatment,there was significant difference in the restoration integrity of the two groups and the incidence of secondary caries (x2 =5.027,P =0.025;secondary caries x2 =4.369,P =0.037).Conclusions The strip crown form of maxillary primary incisors can be used for aesthetic restoration of caries of mandibular primary incisors,and its effect is better than that of direct resin filling.The strip crown form for mandibular primary incisors needs to be developed and produced urgently.

Cardiomyopathies ; etiology ; Humans ; Polymyositis ; complications

Objective To study the clinical effect of orthopedic-orthodontic treatment in crossbite in deciduous dentition through cephalmetric analysis. Methods 12 children with crossbite in deciduous dentition were enrolled in treatment group. Control group included 8 patients with normal deciduous dentition. Modified reverse headgear was used in each patient in the treatment group. Orthodontic force was a-bout 2. 94-4. 90 N (300-500 g) , and the direction of force was under occlusion plane for 37 degree. Each patient wore reverse headgear for 10 hour per day. Orthodontic treatment lasted for 6 months, and then orthodontic treatment began at once. Orthodontic treatment was not over until crossbite was corrected. The cephalmetric change was compared between two groups before treatment (T1) , after orthopedic treatment (T2)and after orthodontic treatment (T3). The effect of orthopedic-orthodontic treatment was analyzed in two group. Results Crossbite was corrected successfully in all patients in the treatment group. After treatment, A point moved forward by 2. 8mm, Is-FHp increased 8. 38 mm, ＜SNA increased 2. 65, incisors in mandible moved backward a little bit. All these changes were statistical different as compared with those in the control group. Conclusion Skeletal and dental deformity are improved progressively after combined orthopedic-orthodontic treatment. It is recommended to perform orthopedic-orthodontic therapy in skeletal crossbite in deciduous dentition.