Objective:To analyze the predictive value of serum transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in patients with non-small cell lung cancer (NSCLC) after single-port thoracoscopic radical resection.Methods:A total of 50 patients with NSCLC who underwent single-port thoracoscopic radical resection in Affiliated Hospital of Xuzhou Medical University from May 2018 to May 2020 were selected as the observation objects. Serum TGF-β1, VEGF levels and Karnofsky functional status (KPS) scores before and after surgery were compared, and the total incidence of complications was calculated. All subjects were followed up for 3 years, and serum levels of TGF-β1, VEGF and KPS scores were compared between relapsed group and non-relapsed group, survival group and death group. Pearson correlation analysis was used to explore the correlation between TGF-β1, VEGF and KPS scores. The receiver operator characteristic (ROC) curve was plotted and the area under the curve (AUC) was calculated to evaluate the predictive value of serum TGF-β1 and VEGF alone and combined detection in patients with NSCLC after single-port thoracoscopic radical resection.Results:The serum levels of TGF-β1 and VEGF were (7.16±1.94) μg/L and (42.26±5.04) ng/L in 50 patients with NSCLC one month after single-port thoracoscopic radical resection, which were lower than those before surgery [ (13.62±3.52) μg/L and (136.52±20.66) ng/L, t=11.37, P<0.001; t=31.34, P<0.001]. The KPS score one month after surgery was 66.57±8.11, which was higher than that before surgery (53.62±5.62, t=9.28, P<0.001). Postoperative wound healing was delayed in 1 of the 50 patients, pulmonary infection in 1 patient, and no pulmonary embolism and other complications occurred. The total incidence of complications was 4.00%. The serum levels of TGF-β1 and VEGF in patients in the relapsed group ( n=6) were (12.95±4.26) μg/L and (72.46±6.05) ng/L respectively, which were higher than those in the non-relapsed group ( n=44) [ (6.37±1.25) μg/L and (38.14±5.37) ng/L; t=8.34, P<0.001; t=29.99, P<0.001]. The KPS score in the relapsed group was 52.16±8.16, which was lower than that in the non-relapsed group (67.55±12.67, t=2.88, P=0.006). Serum levels of TGF-β1 and VEGF in the death group ( n=5) were (13.99±6.82) μg/L and (75.95±9.05) ng/L, which were higher than those in the survival group ( n=45) [ (6.41±3.06) μg/L and (38.52±8.37) ng/L; t=4.56, P<0.001; t=21.47, P<0.001]. The KPS score in the death group was 1.25±0.34, which was lower than that in the survival group (65.11±12.94, t=10.93, P<0.001). Pearson correlation analysis showed that serum levels of TGF-β1 ( r=-0.45, P<0.001) and VEGF ( r=-0.48, P<0.001) were negatively correlated with KPS scores. ROC curve analysis showed that when the optimal cut-off value of TGF-β1 was 8.14 μg/L, the AUC for predicting recurrence after single-port thoracoscopic radical resection was 0.516 (95% CI: 0.446-0.676), the sensitivity was 71.85%, and the specificity was 80.69%. When the optimal cut-off value of VEGF was 142 ng/L, the AUC was 0.659 (95% CI: 0.534-0.761), the sensitivity was 76.04%, and the specificity was 82.52%. The AUC of the combined detection was 0.828 (95% CI: 0.786-0.951), the sensitivity was 91.86%, and the specificity was 87.52%. The AUC of combined detection was higher than that of serum TGF-β1 ( Z=2.63, P=0.007), VEGF ( Z=2.32, P=0.013) single detection. Conclusion:The serum levels of TGF-β1 and VEGF are significantly decreased in NSCLC patients after one month of single-port thoracoscopic radical resection, and the combined detection of the two has predictive value for recurrence after single-port thoracoscopic radical resection.

Objective To investigate the difference in the expression of long non-coding RNA NORAD(LncRNA NORAD)and microRNA-199a-3p(miR-199a-3p)in the serum of patients with esophageal squamous cell carcinoma and healthy people,and analyze their diagnostic value for esophageal squamous cell carcinoma.Methods A total of 286 patients with esophageal squamous cell carcinoma admitted to our hospital from January 2018 to December 22 were included in the esophageal squamous cell carcinoma group,the clinicopathological characteristics were recorded,in the same period,204 healthy people excluding esophageal squamous cell carcinoma and other systemic diseases were included as the healthy control group.The expression levels of serum LncRNA NORAD and miR-199a-3p were detected by real-time fluorescence quantitative PCR(qRT-PCR);Pearson correlation was applied to analyze the correlation between serum LncRNA NORAD and miR-199a-3p expression levels;the diagnostic value of serum LncRNA NORAD and miR-199a-3p expression levels in esophageal squamous cell carcinoma was analyzed by the ROC curve.Results The expression level of serum LncRNA NORAD in esophageal squamous cell carcinoma group was higher than that in healthy control group,the expression level of miR-199a-3p was obviously lower than that in the healthy control group(P＜0.05).There was a negative correlation between serum LncRNA NORAD and miR-199a-3p expression in patients with esophageal squamous cell carcinoma(r=-0.649,P＜0.05).The proportions of high expression of LncRNA NORAD and low expression of miR-199a-3p in patients with lymph node metastasis,TNM stage T3+T4 and poorly differentiated esophageal squamous cell carcinoma were higher than those in patients with no lymph node metastasis,TNM stage T1+T2 and moderately/highly differentiated esophageal squamous cell carcinoma,with statistically obvious difference(P＜0.05).The AUC of LncRNA NORAD and miR-199a-3p in the diagnosis of esophageal squamous cell carcinoma was 0.817,0.796 and 0.854,respectively.Conclusion The serum level of LncRNA NORAD is high and miR-199a-3p is low in patients with esophageal squamous cell carcinoma.LncRNA NORAD and miR-199a-3p can both be used as an auxiliary diagnostic index for esophageal squamous cell carcinoma.