Aim To explore the risk factors for the progressive worsening of moderate coronary stenosis and con-struct a nomogram model for predicting the progression of moderate coronary stenosis based on coronary CT-fractional flow reserve(FFR).Methods 293 patients with moderate coronary stenosis admitted to the hospital from April 2020 to A-pril 2023 were selected as the research subjects.Coronary CT angiography(CCTA)was performed on the selected pa-tients,and CT-FFR software was used to analyze the CCTA images and obtain the FFR values.According to the progres-sive worsening of moderate coronary stenosis,the patients were divided into two groups:the progression group and the sta-ble group,and the data of gender,age,hyperlipidemia,history of cerebral infarction,multivessel disease,obesity,diabe-tes,malnutrition,lack of physical activity,hypertension,drinking,smoking and place of residence of the patients in the two groups were collected.The ten-fold cross-validation in LASSO analysis was used to screen for predictive factors of progressive worsening of moderate coronary stenosis,Logistic regression was used to screen for risk factors of progressive worsening of moderate coronary stenosis,R(4.2.3)was used to establish a nomogram model of progressive worsening of moderate coronary stenosis,and this nomogram model was validated.Results Among 293 patients with moderate cor-onary stenosis,there were 61 cases of progressive worsening,and the incidence of progressive worsening of moderate coro-nary stenosis was 20.82％(61/293).The sex,age,hyperlipidemia,history of cerebral infarction,multi vessel disease,malnutrition,hypertension,alcohol consumption and place of residence of the progression group and the stable group had no statistical significance(P＞0.05),while the proportion of obesity,diabetes,lack of physical activity and smoking in the progression group was significantly higher than those in the stable group,and the FFR was significantly lower than that in the stable group(P＜0.05).LASSO analysis showed that the history of cerebral infarction,obesity,diabetes,lack of physical activity,smoking and FFR were predictive factors with non-zero coefficients.After ten-fold cross-validation,six variables including the history of cerebral infarction,obesity,diabetes,lack of physical activity,smoking and FFR were re-tained into the model.Logistic regression analysis showed that obesity(OR=2.411,95％CI:1.151～5.053),diabetes(OR=3.401,95％CI:1.671～6.923),lack of physical activity(OR=2.818,95％CI:1.427～5.564),smoking(OR=3.577,95％CI:1.526～8.387)were all risk factors for progressive worsening of moderate coronary stenosis,and FFR(OR=0.001,95％CI:0.000～0.036)was the antagonistic factor for progressive worsening of moderate coronary stenosis(P＜0.05).A dynamic nomogram model for the progressive worsening of moderate coronary stenosis was established based on risk factors,and the area under the ROC curve of the nomogram model was 0.777(95％CI:0.711～0.842);The predicted values of the calibration curve are basically consistent with the actual values;When the decision curve showed a threshold probability of 2％to 64％,the nomogram model had a good benefit value for predicting the progressive worsening of moderate coronary stenosis.Conclusion The nomogram model constructed in this study has high accuracy in predicting the progression of moderate coronary stenosis and good clinical practicality.

Aim To explore the risk factors for the progressive worsening of moderate coronary stenosis and con-struct a nomogram model for predicting the progression of moderate coronary stenosis based on coronary CT-fractional flow reserve(FFR).Methods 293 patients with moderate coronary stenosis admitted to the hospital from April 2020 to A-pril 2023 were selected as the research subjects.Coronary CT angiography(CCTA)was performed on the selected pa-tients,and CT-FFR software was used to analyze the CCTA images and obtain the FFR values.According to the progres-sive worsening of moderate coronary stenosis,the patients were divided into two groups:the progression group and the sta-ble group,and the data of gender,age,hyperlipidemia,history of cerebral infarction,multivessel disease,obesity,diabe-tes,malnutrition,lack of physical activity,hypertension,drinking,smoking and place of residence of the patients in the two groups were collected.The ten-fold cross-validation in LASSO analysis was used to screen for predictive factors of progressive worsening of moderate coronary stenosis,Logistic regression was used to screen for risk factors of progressive worsening of moderate coronary stenosis,R(4.2.3)was used to establish a nomogram model of progressive worsening of moderate coronary stenosis,and this nomogram model was validated.Results Among 293 patients with moderate cor-onary stenosis,there were 61 cases of progressive worsening,and the incidence of progressive worsening of moderate coro-nary stenosis was 20.82％(61/293).The sex,age,hyperlipidemia,history of cerebral infarction,multi vessel disease,malnutrition,hypertension,alcohol consumption and place of residence of the progression group and the stable group had no statistical significance(P＞0.05),while the proportion of obesity,diabetes,lack of physical activity and smoking in the progression group was significantly higher than those in the stable group,and the FFR was significantly lower than that in the stable group(P＜0.05).LASSO analysis showed that the history of cerebral infarction,obesity,diabetes,lack of physical activity,smoking and FFR were predictive factors with non-zero coefficients.After ten-fold cross-validation,six variables including the history of cerebral infarction,obesity,diabetes,lack of physical activity,smoking and FFR were re-tained into the model.Logistic regression analysis showed that obesity(OR=2.411,95％CI:1.151～5.053),diabetes(OR=3.401,95％CI:1.671～6.923),lack of physical activity(OR=2.818,95％CI:1.427～5.564),smoking(OR=3.577,95％CI:1.526～8.387)were all risk factors for progressive worsening of moderate coronary stenosis,and FFR(OR=0.001,95％CI:0.000～0.036)was the antagonistic factor for progressive worsening of moderate coronary stenosis(P＜0.05).A dynamic nomogram model for the progressive worsening of moderate coronary stenosis was established based on risk factors,and the area under the ROC curve of the nomogram model was 0.777(95％CI:0.711～0.842);The predicted values of the calibration curve are basically consistent with the actual values;When the decision curve showed a threshold probability of 2％to 64％,the nomogram model had a good benefit value for predicting the progressive worsening of moderate coronary stenosis.Conclusion The nomogram model constructed in this study has high accuracy in predicting the progression of moderate coronary stenosis and good clinical practicality.

The Spt-Ada-Gcn5-acetyltransferase (SAGA) is an ancillary transcription initiation complex which is highly conserved. The ADA1 (alteration/deficiency in activation 1, also called histone H2A functional interactor 1, HFI1) is a subunit in the core module of the SAGA protein complex. ADA1 plays an important role in plant growth and development as well as stress resistance. In this paper, we performed genome-wide identification of banana ADA1 gene family members based on banana genomic data, and analyzed the basic physicochemical properties, evolutionary relationships, selection pressure, promoter cis-acting elements, and its expression profiles under biotic and abiotic stresses. The results showed that there were 10, 6, and 7 family members in Musa acuminata, Musa balbisiana and Musa itinerans. The members were all unstable and hydrophilic proteins, and only contained the conservative SAGA-Tad1 domain. Both MaADA1 and MbADA1 have interactive relationship with Sgf11 (SAGA-associated factor 11) of core module in SAGA. Phylogenetic analysis revealed that banana ADA1 gene family members could be divided into 3 classes. The evolution of ADA1 gene family members was mostly influenced by purifying selection. There were large differences among the gene structure of banana ADA1 gene family members. ADA1 gene family members contained plenty of hormonal elements. MaADA1-1 may play a prominent role in the resistance of banana to cold stress, while MaADA1 may respond to the Panama disease of banana. In conclusion, this study suggested ADA1 gene family members are highly conserved in banana, and may respond to biotic and abiotic stress.
Musa/genetics* ; Phylogeny ; Fungal Proteins ; Cell Nucleus ; Histones ; Stress, Physiological/genetics*

Adipose Tissue ; Adipose Tissue, Brown ; Adipose Tissue, White ; Animals ; Iodine ; Norepinephrine ; Rats ; Tomography, X-Ray Computed

OBJECTIVE: To investigate the effect of the chemoprotectant tempol on the anti-tumor activity of cisplatin (DDP). METHODS: The cellular toxicity of tempol in human colon cancer SW480 cells and mouse colon cancer CT26 cells were evaluated using MTT and cell counting kit-8 assays. CalcuSyn software analysis was used to determine the interaction between tempol and DDP in inhibition of the cell viability. A subcutaneous homograft mouse model of colon cancer was established. The mice were randomly divided into control group, tempol group, cisplatin group and tempol + DDP treatment group with intraperitoneal injections of the indicated agents. The tumor size, body weight and lifespan of the mice were measured, and HE staining was used to analyze the cytotoxic effect of the agents on the kidney and liver. Immunohistochemistry and Western blotting were performed to detect the expression of Bax and Bcl2 in the tumor tissue, and TUNEL staining was used to analyze the tumor cell apoptosis. The level of reactive oxygen species (ROS) in the tumor tissue was determined using flow cytometry. RESULTS: Tempol showed inhibitory effects on the viability of SW480 and CT26 cells. CalcuSyn software analysis showed that tempol had a synergistic anti-tumor effect with DDP (CI < 1). In the homograft mouse model, tempol treatment alone did not produce obvious anti-tumor effect. HE staining showed that the combined use of tempol and DDP alleviated DDP-induced fibrogenesis in the kidneys, but tempol also reduced the anti-tumor activity of DDP. Compared with the mice treated with DDP alone, the mice treated with both tempol and DDP had a significantly larger tumor size ( < 0.01) and a shorter lifespan ( < 0.05). Tempol significantly reversed DDP-induced expression of Bax and Bcl2 in the tumor tissue and tumor cell apoptosis ( < 0.001), and obviously reduced the elevation of ROS level in the tumor tissue induced by DDP treatment ( < 0.05). CONCLUSIONS Tempol can attenuate the anti-tumor effect of DDP while reducing the side effects of DDP. Caution must be taken and the risks and benefits should be carefully weighed when considering the use of tempol as an anti-oxidant to reduce the toxicities of DDP.
Animals ; Antineoplastic Agents ; Antioxidants ; Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; Cisplatin ; Cyclic N-Oxides ; pharmacology ; Drug Resistance, Neoplasm ; Humans ; Mice ; Spin Labels

Objective To obtain the parameters of optimal spectral scanning mode in chest spectral CT imaging,radiation doses,image quality,CNR and subjective scores are compared in spectral and conventional scan modes with anthropomorphic chest phantom.Methods An anthropomorphic chest phantom underwent both conventional scan and spectral scan using three different protocols:GSI assist mode (protocol A);GSI mode with mean tube current (protocol B);GSI mode with maximum tube current (protocol C).All above scans were performed with the noise index (NI) as 9 and 11 respectively.The radiation dose was recorded,and the SD values of adipose tissue and muscle tissue of 5 different sections were measured in regions of interest (ROls) to evaluate the image quality.All scans were subjectively scored in 5 score system by two senior radiologists on lung markings and branches with the lung window technique.Results The effective dose (E) values of conventional CT scan and protocols A,B and C were 8.0,8.5,6.2,10.4 and 5.3,5.1,4.3,6.2 mSv.When NI =9,the difference of SD values had statistically significance between conventional scan and protocols A,C (F =4.496,P ＜ 0.05).When NI =11,there was statistically significant difference of SD value between conventional scan and protocols A,B,C (F =8.425,P ＜ 0.05).In conventional chest scan,the difference of SD values was statistically significant between NI =9 and 11 (t =-2.570,P ＜ 0.05),while other protocols had no statistically significant difference (P ＞ 0.05).The difference of CNR and subjective score had no statistically significance in either the same NI different scanning modes or the same scanning mode with different NIs.Conclusions Appropriate spectral scanning mode had no significant difference with conventional CT scanning in radiation dose,yet higher image quality can be obtained.Furthermore,appropriate NI can reduce the radiation dose obviously while the similar image quality was achieved.Considering the two factors,GSI assist mode can achieve balance between radiation dose and image quality.

Objective To evaluate the nse of CT angiography in the diagnosis of hemoptysis and guiding the treatment of it with 64-slice spiral CT.Methods Twenty-two patients with repeated and massive hemoptysis underwent chest CT angiography.Results The blood supply of hemoptysis was nonbronchial systemic arteries in 3 patients,single or multiple bronchial arteries in 15 patients,mixed arteries of nonbronchial systemic and bronchial arteries in 3 patients and abnormal systemic arteries in 1 patient.Conclusion With 64-slice spiral CT,CT angiography provided useful information for the treatment of hemoptysis by guiding bronchial arterial embolization.