Objective To establish an identification method for Gentiana davidii Franch.Methods Original plants and herbal properties were identified with pharmacognostical method,and its diagnostic characteristics were described.Different tissue parts and whole plant powder of G.davidii Franch.were studied by conventional optical microscopy,scanning electron microscopy combined with various microscopy methods.Results The morphological,trait and microstructure characteristics of G.davidii Franch.were obvious.The microscopic characteristics of the tissues were metazoan cortex visible in the transverse section of the roots,and the casparian strip was obvious in the transverse section of the stems.The epidermis of the leaves showed a cephalic bulge,and the cell wall of the epidermis cells showed a shallow wavy curve.There were stomata and non-glandular hairs in the lower epidermis of the leaves,and the cell walls of the epidermis cells were deeply wavy.The upper epidermis of the petal has a regular arrangement of cerebral striatum spherical protrusions,and the lower epidermis has a regular arrangement of ridge flat elongated protrusions.The pollen grains were oblong with three germination furrows and different sizes of surface decorative mesh.The seed was pear-shaped,the surface was honeycomb,I and II could be seen.Conclusion The microstructure of the features tissue of G.davidii Franch.can provide a basis for its authenticity identification,quality evaluation and formulation of quality standards,and at the same time offer e a new taxonomic basis for the taxonomy of gentian plants.

Objective To establish an identification method for Gentiana davidii Franch.Methods Original plants and herbal properties were identified with pharmacognostical method,and its diagnostic characteristics were described.Different tissue parts and whole plant powder of G.davidii Franch.were studied by conventional optical microscopy,scanning electron microscopy combined with various microscopy methods.Results The morphological,trait and microstructure characteristics of G.davidii Franch.were obvious.The microscopic characteristics of the tissues were metazoan cortex visible in the transverse section of the roots,and the casparian strip was obvious in the transverse section of the stems.The epidermis of the leaves showed a cephalic bulge,and the cell wall of the epidermis cells showed a shallow wavy curve.There were stomata and non-glandular hairs in the lower epidermis of the leaves,and the cell walls of the epidermis cells were deeply wavy.The upper epidermis of the petal has a regular arrangement of cerebral striatum spherical protrusions,and the lower epidermis has a regular arrangement of ridge flat elongated protrusions.The pollen grains were oblong with three germination furrows and different sizes of surface decorative mesh.The seed was pear-shaped,the surface was honeycomb,I and II could be seen.Conclusion The microstructure of the features tissue of G.davidii Franch.can provide a basis for its authenticity identification,quality evaluation and formulation of quality standards,and at the same time offer e a new taxonomic basis for the taxonomy of gentian plants.

The theory of "liver opens at the eyes" was first seen in Yellow Emperor's Internal Canon of Medicine， which is the ancient people's summary of the connection between the liver and the eyes. The theory of "liver opens at the eyes" suggests the characteristic of "co-damage and co-recover of liver and eyes". It has been found in clinical practice that liver diseases and eye diseases often occur together， and "liver and eyes co-recover" is an ideal choice. The key to achieving "liver and eyes co-recover" is to analyze its pharmacological material basis and mechanism. With the development of modern medicine， more and more evidence indicates that the liver and eyes have complex and close relationships in physiological and pathological aspects. In a pathological state， there is a phenomenon of "liver and eyes co-damage"， and after the intervention of traditional Chinese medicine， "liver and eyes co-recover" occurs. "Liver and eyes co-damage and co-recover" can be explained through the "co-material basis and co-action mechanism". On this basis， the research group tentatively proposed that the liver and eyes had "four common characteristics" （4CCs）， namely "co-damage， co-recover， co-material basis， and co-action mechanism" from the theoretical connotation of traditional Chinese medicine， clinical practice， and molecular biology. Additionally， the group also took the intervention of Prunella vulgaris， traditional Chinese medicine， for removing liver fire and improving eyesight on immune liver injury （ILI） and allergic conjunctivitis （AC） as examples to analyze 4CCs. This project aims to deeply analyze the scientific connotation of the theory of "liver opens at the eyes"， reveal the common characteristics and biological essence of liver and eyes， explore a new research paradigm of "liver and eyes co-recover"， and provide a reference for the study of common problems of multi-organ associated diseases.

Objective To conduct bioinformatics analysis of children with severe malaria to find out the key gene changes in order to provide a new basis for the prevention and treatment of malignant malaria.Methods Microarray gene chip data was downloaded from public databases GEO and imported into the analysis software STRING,PANTHER and GenClip.The gene expression profiles,protein interaction networks,the process of molecular biology,gene function were analyzed.Results 623(1.93 ％) differentially expressed genes had a good diagnostic capabilities in the diagnosis of mild and severe malaria.OAS2,OAS3,IFIT3 and USP18 were the core sub-network node of the Protein-Protein Interactions.Differentially expressed genes mainly involved in the body's immune defense,immune response,response to external stimuli,the biological function of type 1 interferon activation pathways.Conclusion The progress of malaria of children may be in the regulation of OAS2,OAS3,IFIT3,USP18 and children's immune defense capacity decreased,the malaria began to progress more easily.