Objective To analyze the predictive effect of miRNA-let-7 combined with serum markers on the chemotherapy effect and prognosis of glioma(GBM)patients.Methods We collected the data of 25 patients with GBM.Twenty healthy volunteers were included as the control group.Fasting serum samples were collected.The levels of let-7b,let-7d,and let-7e in serum samples were analyzed by quantitative real-time PCR.Results Compared with the control group,the levels of let-7b(192.5±18.71 vs.98.13±11.68,t=6.42,P<0.0001)and let-7d(82.9±14.14 vs.44.62±6.83,t=2.41,P<0.05)and let-7e(139.60±14.62 vs.75.86±9.72,t=5.45,P<0.001)in the peripheral blood of patients in the GBM group decreased significantly.The let-7b and let-7e levels significantly decreased in GBM patients over 60 years old,with tumor size over 5 cm,non-frontotemporal lesions,and ECOG of 2,who underwent biopsy alone(P<0.05).The levels of let-7b and let-7e significantly differed among GBM patients with various types of treatment response(P<0.05).The OS of GBM patients with high expression of let-7b and let-7e was higher than that of patients with low let-7b and let-7e expression(P<0.05).Conclusion The expression of let-7b and let-7e in blood samples can be used to monitor GBM and predict patients'response to treatment and prognosis.

Objective To explore the correlation between erythrocyte sedimentation rate(ESR)and cellular immunity in children with Mycoplasma pneumoniae pneumonia(MPP)of different ages.Methods 195 children diagnosed with Mycoplasma pneumoniae pneumonia admitted to our hospital from January 2020 to December 2023 were selected as the study subjects.All patients were divided into three groups based on age:74 infants and young children(≤3 years old),63 young children(3-6 years old),and 58 older children(≥6 years old).Compare clinical data,imaging information,and cellular immune levels of children of different age groups,and analyze the correlation between ESR levels and cellular immune levels through EmpowerStats stratified interaction test.MMP patients were divided into RRTI group(n=63)and non RRTI group(n=132)based on whether they had recurrent respiratory tract infection(RRTI).Clinical data of the two groups were compared,and the Cox proportional hazards model was used to analyze the correlation between ESR levels and the occurrence of RRTI in MPP patients at different age groups.ESR levels were divided into a quintile array from low to high,and a subgroup analysis was conducted on the correlation between ESR levels and the occurrence of RRTI in MPP patients.The restricted cubic spline model was used to analyze the dose-response relationship between ESR levels and the occurrence of RRTI in MPP patients.Results Children of different ages had fever peaks,fever duration,persistent high fever>7 days,difficulty breathing,asthma,wet rales,wheezing,extrapulmonary complications,neutrophil count(ESR),Eosinophil count,C-reactive protein(CRP),lactate dehydrogenase(LDH),D-dimer,and whether RRTI were present were statistically significant(P<0.05).Compared with MPP patients in the infant and toddler group,the incidence of lobular parenchymal infiltration,interstitial infiltration,and CD8+levels in the younger and older infant groups were significantly reduced(P<0.05),while the incidence of pulmonary parenchymal infiltration,pleural effusion,CD3+,CD4+levels,and CD4+/CD8+ratio levels were significantly increased(P<0.05).EmpowerStats stratified interaction test analysis showed that after adjusting for other factors such as heat peak,heat duration,and dyspnea,the relationship between ESR levels and cellular immune indicators still exists in MPP patients of different ages.There were significant differences in age,age group,heat peak,ESR,CRP,Fibrinogen(Fib),D-dimer,lobular parenchymal infiltration,segmental parenchymal infiltration,atelectasis,pleural effusion,CD3+,CD4+,CD8+,and CD4+/CD8+ratio between the RRTI and non RRTI groups(P<0.05).The Cox proportional risk model results showed that after adjusting for variables such as CRP and pleural effusion,ESR levels remained a protective factor for RTTI in MPP patients,and there was interaction between subgroups.The results of the restricted cubic spline model show that there is no non-linear dose-response relationship between ESR levels and RRTI occurrence in MPP patients of different ages.Conclusion There is a correlation between ESR levels and cellular immunity in children with MPP of different ages,and the relationship is stable.ESR level is a protective factor for MPP patients with RRTI.

Objective To explore the correlation between erythrocyte sedimentation rate(ESR)and cellular immunity in children with Mycoplasma pneumoniae pneumonia(MPP)of different ages.Methods 195 children diagnosed with Mycoplasma pneumoniae pneumonia admitted to our hospital from January 2020 to December 2023 were selected as the study subjects.All patients were divided into three groups based on age:74 infants and young children(≤3 years old),63 young children(3-6 years old),and 58 older children(≥6 years old).Compare clinical data,imaging information,and cellular immune levels of children of different age groups,and analyze the correlation between ESR levels and cellular immune levels through EmpowerStats stratified interaction test.MMP patients were divided into RRTI group(n=63)and non RRTI group(n=132)based on whether they had recurrent respiratory tract infection(RRTI).Clinical data of the two groups were compared,and the Cox proportional hazards model was used to analyze the correlation between ESR levels and the occurrence of RRTI in MPP patients at different age groups.ESR levels were divided into a quintile array from low to high,and a subgroup analysis was conducted on the correlation between ESR levels and the occurrence of RRTI in MPP patients.The restricted cubic spline model was used to analyze the dose-response relationship between ESR levels and the occurrence of RRTI in MPP patients.Results Children of different ages had fever peaks,fever duration,persistent high fever>7 days,difficulty breathing,asthma,wet rales,wheezing,extrapulmonary complications,neutrophil count(ESR),Eosinophil count,C-reactive protein(CRP),lactate dehydrogenase(LDH),D-dimer,and whether RRTI were present were statistically significant(P<0.05).Compared with MPP patients in the infant and toddler group,the incidence of lobular parenchymal infiltration,interstitial infiltration,and CD8+levels in the younger and older infant groups were significantly reduced(P<0.05),while the incidence of pulmonary parenchymal infiltration,pleural effusion,CD3+,CD4+levels,and CD4+/CD8+ratio levels were significantly increased(P<0.05).EmpowerStats stratified interaction test analysis showed that after adjusting for other factors such as heat peak,heat duration,and dyspnea,the relationship between ESR levels and cellular immune indicators still exists in MPP patients of different ages.There were significant differences in age,age group,heat peak,ESR,CRP,Fibrinogen(Fib),D-dimer,lobular parenchymal infiltration,segmental parenchymal infiltration,atelectasis,pleural effusion,CD3+,CD4+,CD8+,and CD4+/CD8+ratio between the RRTI and non RRTI groups(P<0.05).The Cox proportional risk model results showed that after adjusting for variables such as CRP and pleural effusion,ESR levels remained a protective factor for RTTI in MPP patients,and there was interaction between subgroups.The results of the restricted cubic spline model show that there is no non-linear dose-response relationship between ESR levels and RRTI occurrence in MPP patients of different ages.Conclusion There is a correlation between ESR levels and cellular immunity in children with MPP of different ages,and the relationship is stable.ESR level is a protective factor for MPP patients with RRTI.

ObjectiveTo investigate the effects of visual motion-induced brain-computer interface (BCI) technology on upper limb motor function and cognitive function of patients with stroke. MethodsFrom July, 2021 to March, 2022, 50 stroke patients with upper limb hand dysfunction in Shaanxi Provincial Rehabilitation Hospital were randomly divided into control group (n = 25) and experimental group (n = 25). Both groups received conventional rehabilitation therapy, in addition, the control group received passive rehabilitation training, and the experimental group received visual motion-induced BCI rehabilitation training, for two weeks. They were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE), modified Barthel Index (MBI) and Montreal Cognitive Assessment (MoCA) before and after treatment. Brain participation was evaluated during the whole training process of the experimental group. ResultsBefore treatment, there was no difference in the scores of FMA-UE, MBI and MoCA between two groups (P > 0.05). Two weeks after treatment, the scores of FMA-UE, MBI and MoCA improved in both groups (t > 2.481, P < 0.001), and were better in the exprimental group than in the control group (t > 2.453, P < 0.05); the mean brain participation of the experimental group increased 21% after treatment. ConclusionVisual motion-induced BCI rehabilitation training could promote the recovery of motor function of upper limb, and cognitive function of patients with stroke.

In recent years, the IVD industry has developed rapidly based on the increasing market demand, and plays an important role in disease prevention, clinical diagnosis, health monitoring and guiding treatment. Therefore, followed quality and safety issues are highly concerned. The unique advantages of blockchain technology, decentralization, distrust and non-tampering, can write into trusted node data in every link covering production, circulation and usage of IVD reagents, and establish a distributed ledger with full backup, which makes the anti-conterfeiting and traceability for IVD reagents possible. We discuss whole process intelligent tracing system for IVD reagents based on blockchain technology. Through the strong mechanism of pre-supervision and post-punishment, the source of reagents can be traced, quality and responsibility can be investigated, and the medical inspection quality and diagnostic safety can be guarded.
Blockchain ; Indicators and Reagents ; Reagent Kits, Diagnostic ; Technology

Objective:To explore effects of multidisciplinary team cooperation combined with continuous nursing on postoperative functional recovery of patients with high cervical spinal cord tumors.Methods:Using the convenient sampling method, a total of 60 patients with high cervical spinal cord tumors who were hospitalized in Department of Orthopedics of the First Affiliated Hospital of Xinjiang Medical University were selected from January 2012 to December 2019. According to the time of admission, they were divided into the control group and the observation group, with 30 cases in each group. The observation group adopted multidisciplinary team cooperation combined with continuous nursing model, while the control group adopted conventional nursing. Function recovery, muscle strength and complications were compared between the two groups at 3 months after operation.Results:There were 18 cases with good function recovery in the observation group and 10 cases with good function recovery in the control group, and the difference was statistically significant (χ 2=4.268, P<0.05) . The muscle tension was improved in 17 cases of observation group and 6 cases of control group, and the difference was statistically significant (χ 2=8.684, P<0.05) . Complications occurred in 2 patients in the observation group and 9 patients in the control group, the difference was statistically significant (χ 2=5.455, P<0.05) . Conclusions:Multidisciplinary team cooperation combined with continuous nursing can promote postoperative rehabilitation of patients with high cervical spinal cord tumors, and reduce complications.

Objective: To observe the effects of continuous nursing(CN) on rehabilitation of patients with hypertensive intracerebral hemorrhage (HICH) and to explore the effective interventive measures for CN. Methods: Totally 129 admitted HICH patients from January to December 2017 were selected and randomly divided into study group (63 cases) and control group (66 cases), the control group was handed with Handbook of stroke prevention and control before discharge, the study group received CN intervention, which included pre-discharge health education, family visits, patient management and video interview via WeChat App, group lecture and psychological support, self-nursing ability, motor function and ability of daily life and emotional condition were evaluated before and post discharge, and the disabled ratio 6 months after discharge between the 2 groups were compared. Results: The 2 groups with the scores of exercise of self-care agency scale (ESCA) were increased significantly compared with before discharge (all P<0.05) ; the study group with the ESCA scores 3 months and 6 months after discharge were significantly higher than the control group [3months:118.5±8.2 vs. 112.3±7.5; 6 months: 127.7±8.7 vs. 119.3±9.1] (all P<0.05) . Fugl Meyer score and Barthel index of the 2 group after discharge were both lower than the pre-discharge levels (all P<0.05); Fugl Meyer score and Barthel index of the study group 3 months and 6 months after discharge were significantly higher than the control group [3 months: Fugl Meyer score 73.3±7.3 vs. 69.4±6.9; Barthel index 56.5±8.0 vs. 51.8±7.3; 6 months: Fugl Meyer score77.6±8.0 vs. 74.5±7.2; Barthel index 67.5±8.7 vs. 63.0±7.4] (all t=2.291-3.454, P<0.05). Two groups with HAD score after discharge were significantly decreased compared with before discharge (P<0.05); the study group with the HAD-A and HAD-D scores 3 and 6 months after discharge were significantly lower than the control group[3 months: HAD-A 6.75±2.23 vs. 8.02±2.85; HAD-D 6.07±1.75 vs. 7.23±1.94; 6 months: HAD-A 5.93±2.04 vs. 6.84±2.37; HAD-D 5.86±1.47vs. 6.75±1.76] (all P<0.05) . The study group with the re-admission rate and disabled ratio within 6 months after discharge were both lower than the control group, but the differences without statistical significance (all P>0.05). Conclusion The HICH patients added with CN can significantly improve self-care ability, improve prognosis; patient management via WeChat App and group lecture & psychological support are effective measures to carry out CN.

Objective To study the long-term follow-up results of Ross surgery.Methods Between October 1994 and February 2009,58 consecutive patients underwent a Ross procedure at our institution.The right ventricular outflow tract was repaired with a cryopreserved pulmonary homograft.All patients were scheduled for a yearly study thereafter that ended at the time of death or at closure of the follow-up visit.Mean follow-up was (12.6 ± 5.8) years (range:4.5 to 18.2 years).Results There were two early deaths (3 ％)and one late death (2％).Acturial survival at 16 years was (94.8 ± 3.1) ％.One patient required reoperation 1.5 years after his first Ross operation.Of the 55 survival patients,42 patients(76％) were in NYHA functional class Ⅰ and 13 patients(24％) were in NYHA functional class Ⅱ.Grade 1 or grade 2 autograft regurgitation was observed in seven patients(12％) at one year after the surgery.The sinus of Valsalva diameters were all ＜ 40 mm in these seven patients.Mter surgery,freedom from hemodynamically relevant autograft regurgitation was(87.9 ± 2.8) ％ at 16 years,whose left ventricular function was significantly improved and left ventricular end-diastolic diameter recovered to normal over the long term.None of the patients required reoperation due to pulmonary homograft failure.Conclusion The Ross procedure can be safely performed in patients with aortic valve disease.To date,mortality,morbidity,and reoperation relates are very low.Reasons for these superior results may include the following:diameter of the aortic valve annulus matching that of the pulmonary valve and patients were monitored with antihypertensive medications to keep systolic blood pressure under 120mmHg(1 mmHg =0.133kPa) to delay pressure lesions to the pulmonary autograft.

Objective To investigate nursing interns’knowledge and attitude towards needle stick injury before clinical practice.Methods In 20-23 June,2015,nursing interns who were about to start clinical practice in a hospi-tal were investigated,nursing interns’knowledge and attitude towards needle stick injury were surveyed through questionnaire.Results A total of 350 questionnaires were distributed,324 (92.57%)responded questionnaires were available.40(12.35%)questionnaires were responded by male interns,and 284(87.65%)were by female in-terns;34(10.49%)interns had bachelor degree;the mean age of interns were (20.83 + 1 .24)years old.The correct answer rates about questions related to injury occurring during needle recapping and wearing gloves were low (about 60%).About 70% of the interns gave the correct answers to questions about hepatitis B infection due to needle stick injury following hepatitis B vaccination,as well as medication after injury.Score for individual question about attitude towards needle stick injury was ≤3,the major related problems were susceptibility of blood borne diseases and recapping needles.Conclusion Needle stick injury-related knowledge and attitude among nursing interns is inadequate,including recapping needles,timely report,susceptible to infectious diseases,and so on.It is necessary for schools and teaching hospitals to strengthen the education about occupational protection among nursing interns, so as to improve the attitude and ability of professional protection.

Objective To study the relationship between heart rate and failure of biological valve,and whether reduce the heart rate could delay the failure of biological valve.Methods Retrospective analysis of 92 bioprosthetic valve replacement cases in our institution from April 1996 to March 2001.The follow-up was carried out through the outpatient service,telephone and letter.The patients were divided into two groups based on the heart rate:Group A:basic heart rate no more than 75 beats per minute;Group B:basic heart rate greater than 75 beats per minute.Blood pressure,heart function,echocardiogram and reoperation rate was collected.Results In group A,the average follow-up time and the longest follow-up time was better than the patients in group B but has no significant difference.Echocardiographic data showed that the proportion of valve leaflets stiffness and calcification in group A was significantly lower than group B(7.7％ vs.17.9％,P ＜0.05).Redo operation rate in group A was lower than those in group B (7.7％ vs.22.6％,P ＜ 0.05).There were only 1 case(1.5 ％) suffering from the tearing in all three valve leaflets in group A while it was 8 cases(8.7％) in group B (2.6％ vs.15.1％,P ＜ 0.05).Conclusion Basic heart rate has a significant effect on the failure of the mitral bioprosthesis.To decrease the basic heart rate can delay the failure of the mitral bioprosthesis and improve the durability.