OBJECTIVE To investigate the ameliorative effects of Coptidis Rhizoma-Ophiopogonis Radix herb pair （HLMD） in rats with type 2 diabetes mellitus （T2DM） and its potential mechanisms.METHODS Male Wistar rats were randomly divided into a control group （6 rats） and a model induction group （30 rats）. The T2DM rat model was established by feeding a high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin. The successfully modeled rats were subsequently divided into the model group， the metformin group （200 mg/kg）， and the low-， medium-， and high-dose HLMD groups （100， 200 and 400 mg/kg， respectively）， with six rats in each group. Rats in each group were administered the corresponding drug solution or normal saline by oral gavage once daily for six consecutive weeks. Body weight， fasting blood glucose （FBG）， the area under the curve （AUC） of the oral glucose tolerance test， serum lipid profiles [total cholesterol （TC）， triglyceride （TG）， low-density lipoprotein cholesterol （LDL-C）， and high-density lipoprotein cholesterol （HDL-C）]， and inflammatory cytokine [interleukin-6 （IL-6）， IL-1β， tumor necrosis factor-α （TNF-α）， and IL-10] levels were measured. Histopathological changes in liver and pancreatic tissues， as well as hepatic lipid accumulation， were evaluated. Changes in gut microbiota composition were analyzed in the control， model， and high-dose HLMD groups. Network pharmacology and molecular docking analyses were performed to identify potential therapeutic targets of HLMD against T2DM， followed by experimental validation.RESULTS Compared with the model group， the body weight generally recovered in rats in all HLMD dose groups， while FBG levels consistently decreased. Histopathological abnormalities， including disrupted pancreatic islet architecture， disorganized hepatocyte arrangement， hepatic cord necrosis， and lipid accumulation， were alleviated to varying degrees. AUC （except for the low-dose group）， levels of TC， TG， LDL-C， IL-6（except for the low-dose group）， IL-1β and TNF-α， Simpson and Shannon indices， as well as the relative abundances of Firmicutes， Proteobacteria， and Clostridium were significantly reduced； meanwhile， the levels of HDL-C（except for the low-dose group） and IL-10 （except for the low-dose group）， as well as the relative abundances of Bacteroidota and Ligilactobacillus were significantly increased （ P ＜0.05）. Network pharmacology and molecular docking analyses suggested that the active constituents of HLMD， including quercetin， adenosine， oleanolic acid， stigmasterol， and ophiopogonin D′， may exert antidiabetic effects by targeting key proteins such as protein kinase B1（Akt1）， TNF， IL-6， P53 and IL-1β. Experimental validation further demonstrated that HLMD significantly downregulated the protein expressions of P53 （except for the low-dose group）， IL-6 （except for the low-dose group）， IL-1β and TNF-α in the liver tissues of T2DM rats （ P ＜0.05）.CONCLUSIONS HLMD alleviates glucose and lipid metabolism disorders， attenuates inflammatory responses， and ameliorates hepatic and pancreatic tissue damage in T2DM rats. These beneficial effects may be associated with the modulation of gut microbiota composition and the downregulation of inflammation-related targets， including P53， IL-6， IL-1β and TNF-α.

Objective:To establish a method for the rapid detection of varicella-zoster virus (VZV) by recombinase-aid amplification (RAA) combined with Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system.Methods:Clinical samples of suspected herpes zoster in Shandong province and Shanghai from 2023 to 2024 were collected, nucleic acids of positive samples were extracted, RAA-specific primers and crRNA (CRISPR RNA, crRNA) were designed for the conserved region of VZV, and the fluorescence intensity generated by Cas12a non-specific cleavage of single-stranded fluorescent probes was used to screen highly sensitive crRNAs and optimize the concentrations of crRNA, Cas12a and ssDNA probes. The sensitivity and reproducibility of the RAA-CRISPR/Cas12a detection method were evaluated by using synthesized plasmids and clinical samples, and the specificity of the method was evaluated by using other viral nucleic acids. The method was used to detect clinical samples by using the method and quantitative real-time PCR (qPCR) method, and the detection rate and consistency of the two method were compared.Results:The highly sensitive crRNA-4 was screened from the four crRNAs designed, and a VZV detection method for RAA-CRISPR/CAS12a based on fluorescence intensity measurement was established, which could be detected at 37℃ in 45 min, and the sensitivity of the detection could reach 10 copies/μL, a minimum clinical sample with a Ct value of 38.980 can be detected. It has high specificity and no cross-reactivity with Adenovirus 7, Herpes simplex virus type I, Herpes simplex virus type II, Coxsackieviruses A16, Cytomegalovirus, Epstein-Barr virus, Measles virus, Mumps virus, Enterovirus 71, Japanese encephalitis virus genotype 5. It has good stability, and can be successfully detected in low, medium and high concentrations of viral positive plasmids with good consistency. The detection rate of the clinically positive samples was 100%, which was completely consistent with the qPCR test result.Conclusions:RAA isothermal amplification technology combined with CRISPR-CAS12a technology was used to establish an accurate method for the detection of VZV virus, which was highly sensitive, specific, and had low requirements for experimental conditions, and could be completed within 45 min, which could provide strong technical support for the early detection of VZV.

To summarize the nursing experience of a case involving hemorrhagic shock caused by broad ligament hematoma in vaginal delivery after cesarean section.Nursing points include:recognition and first aid for occult postpartum haemorrhage;precise management of blood transfusion and volume resuscitation;objective-oriented temperature management strategy;postoperative observation and care for abdominal packing;provision of breastfeeding support in case of separation of mother and child;comprehensive psychological nursing support throughout all stages.Following active treatment and nursing interventions,the patient was discharged 10 d after surgery.

To summarize the nursing experience of a case involving hemorrhagic shock caused by broad ligament hematoma in vaginal delivery after cesarean section.Nursing points include:recognition and first aid for occult postpartum haemorrhage;precise management of blood transfusion and volume resuscitation;objective-oriented temperature management strategy;postoperative observation and care for abdominal packing;provision of breastfeeding support in case of separation of mother and child;comprehensive psychological nursing support throughout all stages.Following active treatment and nursing interventions,the patient was discharged 10 d after surgery.

Establishing occupational health related files is a key component of building an occupational health and safety management system in laboratory animal institutions.These files not only serve as a critical means of protecting the legitimate rights and interests of laboratory animal personnel but also provide institutional safeguards.According to relevant laws and regulations,laboratory animal institutions are responsible for establishing and properly maintaining occupational health surveillance files(also known as occupational hygiene files)for both the institution and its personnel.In accordance with the Occupational Health Archives Management Standards,laboratory animal institutions are required to maintain at least seven categories of occupational health management archive folders:the"three simultaneous"occupational health archives for construction projects;occupational health management folders;occupational health publicity and training folders;folders for monitoring and evaluating occupational disease hazard factors;employer occupational health surveillance folders;individual occupational health surveillance folders for employees;and other relevant folders.Building upon this foundation,the Regulations on the Supervision and Administration of Occupational Health in the Workplaces further specify detailed requirements for occupational health files.These include:documents outlining the responsibility system for occupational disease prevention and control;occupational hygiene management systems and standard operating procedures,lists of types of occupational disease hazard factors in workplaces,their distribution by job distribution,and records of work exposure,basic information on occupational disease prevention and emergency rescue facilities,along with records of their allocation,usage,maintenance,inspection and replacement;records concerning the provision,distribution,maintenance and replacement of personal protective equipment(PPE);and reports on occupational disease hazard incidents;and corresponding emergency response records—amounting to twelve specific requirements in total.In short,occupational health management files are a comprehensive collection of data and documentation related to workers'occupational health.Based on the author's practical experience,this paper outlines the main types of occupational health management files,their basic contents,and key points in their development.It also offers a detailed analysis of how to establish such files,aiming to support laboratory animal institutions in successfully developing occupational health management archives and improving their occupational health and safety management systems.

Objective:To establish a method for the rapid detection of varicella-zoster virus (VZV) by recombinase-aid amplification (RAA) combined with Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system.Methods:Clinical samples of suspected herpes zoster in Shandong province and Shanghai from 2023 to 2024 were collected, nucleic acids of positive samples were extracted, RAA-specific primers and crRNA (CRISPR RNA, crRNA) were designed for the conserved region of VZV, and the fluorescence intensity generated by Cas12a non-specific cleavage of single-stranded fluorescent probes was used to screen highly sensitive crRNAs and optimize the concentrations of crRNA, Cas12a and ssDNA probes. The sensitivity and reproducibility of the RAA-CRISPR/Cas12a detection method were evaluated by using synthesized plasmids and clinical samples, and the specificity of the method was evaluated by using other viral nucleic acids. The method was used to detect clinical samples by using the method and quantitative real-time PCR (qPCR) method, and the detection rate and consistency of the two method were compared.Results:The highly sensitive crRNA-4 was screened from the four crRNAs designed, and a VZV detection method for RAA-CRISPR/CAS12a based on fluorescence intensity measurement was established, which could be detected at 37℃ in 45 min, and the sensitivity of the detection could reach 10 copies/μL, a minimum clinical sample with a Ct value of 38.980 can be detected. It has high specificity and no cross-reactivity with Adenovirus 7, Herpes simplex virus type I, Herpes simplex virus type II, Coxsackieviruses A16, Cytomegalovirus, Epstein-Barr virus, Measles virus, Mumps virus, Enterovirus 71, Japanese encephalitis virus genotype 5. It has good stability, and can be successfully detected in low, medium and high concentrations of viral positive plasmids with good consistency. The detection rate of the clinically positive samples was 100%, which was completely consistent with the qPCR test result.Conclusions:RAA isothermal amplification technology combined with CRISPR-CAS12a technology was used to establish an accurate method for the detection of VZV virus, which was highly sensitive, specific, and had low requirements for experimental conditions, and could be completed within 45 min, which could provide strong technical support for the early detection of VZV.

Establishing occupational health related files is a key component of building an occupational health and safety management system in laboratory animal institutions.These files not only serve as a critical means of protecting the legitimate rights and interests of laboratory animal personnel but also provide institutional safeguards.According to relevant laws and regulations,laboratory animal institutions are responsible for establishing and properly maintaining occupational health surveillance files(also known as occupational hygiene files)for both the institution and its personnel.In accordance with the Occupational Health Archives Management Standards,laboratory animal institutions are required to maintain at least seven categories of occupational health management archive folders:the"three simultaneous"occupational health archives for construction projects;occupational health management folders;occupational health publicity and training folders;folders for monitoring and evaluating occupational disease hazard factors;employer occupational health surveillance folders;individual occupational health surveillance folders for employees;and other relevant folders.Building upon this foundation,the Regulations on the Supervision and Administration of Occupational Health in the Workplaces further specify detailed requirements for occupational health files.These include:documents outlining the responsibility system for occupational disease prevention and control;occupational hygiene management systems and standard operating procedures,lists of types of occupational disease hazard factors in workplaces,their distribution by job distribution,and records of work exposure,basic information on occupational disease prevention and emergency rescue facilities,along with records of their allocation,usage,maintenance,inspection and replacement;records concerning the provision,distribution,maintenance and replacement of personal protective equipment(PPE);and reports on occupational disease hazard incidents;and corresponding emergency response records—amounting to twelve specific requirements in total.In short,occupational health management files are a comprehensive collection of data and documentation related to workers'occupational health.Based on the author's practical experience,this paper outlines the main types of occupational health management files,their basic contents,and key points in their development.It also offers a detailed analysis of how to establish such files,aiming to support laboratory animal institutions in successfully developing occupational health management archives and improving their occupational health and safety management systems.

ObjectiveTo observe the effect of Coptidis Rhizoma and Ophiopogonis Radix on renal tissue injury and epidermal growth factor receptor （EGFR）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway in rats with diabetic nephropathy （DN） and explore its possible mechanism of delaying DN. MethodThirty-six male Wistar rats were randomly divided into a normal group （6 rats） and a model group （30 rats）. The model group was fed with a high-fat and high-sugar diet combined with streptozotocin （STZ） to establish a rat model of type 2 diabetes. After the successful preparation of the model， the rats were randomly divided into the model group， low， medium， and high dose groups of Coptidis Rhizoma and Ophiopogonis Radix （100， 200， 400 mg·kg^-1）， and metformin group （200 mg·kg^-1）. After administration， the levels of fasting blood glucose （FBG）， 24 h urine protein （24 h-UTP）， creatinine （SCr）， urea nitrogen （BUN）， and uric acid （UA） were detected. Hematoxylin-eosin （HE） staining and Masson staining were used to observe the pathological changes of renal tissue in rats. Western blot and Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） were used to detect the related protein expression of EGFR， PI3K， and Akt and their mRNA expression levels in the renal tissue of rats in each group. ResultsCompared with the normal group， the levels of FBG， SCr， BUN， UA， 24 h-UTP， and kidney index in the model group were significantly increased （P<0.01）， most renal tubular epithelial cells were necrotic， and the content of collagen in glomeruli was significantly increased （P<0.01）. Compared with the model group， the above indexes of rats in each administration group were improved to varying degrees. The FBG， SCr， BUN， UA， 24 h-UTP， and kidney index of rats in each dose group and metformin group were significantly decreased （P<0.01， P<0.05）. The necrosis degree of renal tubular epithelial cells was reduced， and the fibrosis area was decreased （P<0.01）. There related protein and mRNA expressions of EGFR， PI3K， and Akt were significantly increased （P<0.05， P<0.01）. ConclusionCoptidis Rhizoma and Ophiopogonis Radix can alleviate renal tissue injury in rats with DN， and their mechanism may be related to the regulation of the EGFR/PI3K/Akt signaling pathway.

OBJECTIVE To prepar e and characterize danazol （DAZ）-sodium caseinate （SC）composite nanoparticles ，and to study the mechanism of preparing nanoparticles in “bottom-up”technology. METHODS SC was used as a stabilizer for regulating nanoparticles，so that DAZ-SC composite nanoparticles were prepared by anti-solvent precipitation method. The particle size ， Zeta-potential，micro-morphology，stability，encapsulation efficiency ，drug loading and in vitro dissolution rate were characterized. Fluorescence spectra ，IR spectra ，FBRM and other methods were used to analyze the interaction mechanism between DAZ and SC. RESULTS The particle size of DAZ-SC composite nanoparticles was （223.7±12.5）nm，and the polydispersity index was 0.274± 0.012. Zeta-potential was －（17.81±1.63）mV（n＝3）. The stability of nano-suspension was good ，the solid properties of DAZ were greatly improved ，and the dissolution rate was significantly increased. SC was statically quenched under the action of DAZ and the secondary structures of SC were changed. The crystallization process of DAZ was controlled under the action of SC ，and the interaction between DAZ and SC was mainly hydrogen bond and van der Waals force. CONCLUSIONS In this study ，DAZ-SC composite nanoparticles are successfully prepared. In the “bottom-up”technology，the interaction between SC and DAZ caused by hydrogen bond and van der Waals force inhibits the growth and agglomeration of drug crystals .

Objective:To explore the effect of supportive-expressive group therapy (SEGT) on alexithymia in patients with lung cancer.Methods:Totally 62 lung cancer patients were divided into control group( n=30) and intervention group( n=32) by coin flipping method.The intervention group received 6-week supportive-expressive group therapy and the intervention path was improved by action research method.There was no special intervention in the control group.The Toronto alexithymia scale (TAS-20) and hospital anxiety and depression scale (HADS) were used for measurement, and statistical analysis was carried out by SPSS 21.0. Results:The emotional recognition disorder (Wald groupχ 2=6.055, Wald interactionχ 2=15.157), emotional description disorder (Wald groupχ 2=5.736, Wald interactionχ 2=28.912), TAS-20 (Wald groupχ 2=7.181, Wald interactionχ 2=28.126), anxiety (Wald groupχ 2=4.905, Wald interactionχ 2=30.491), depression (Wald groupχ 2=9.580, Wald interactionχ 2=29.417) and HADA (Wald groupχ 2=8.140, Wald interactionχ 2=47.851) had significant group effect and interaction effect at the pre-test, post-test and 1 month after intervention(all P<0.05). There was no significant group effect in extroverted thinking (Wald groupχ 2=1.161, P>0.05), but the time effect (Wald timeχ 2=6.381, P<0.05) and the interaction effect (Wald interactionχ 2=6.339, P<0.05) were significant in the three time points.The group effect of emotional recognition disorder, emotional description disorder, TAS-20, anxiety, depression and HADA score at completed intervention and 1 month after intervention were significant(all P<0.05). Compared with before intervention, TAS-20((52.94±4.77) , (52.06±4.07)), emotional recognition disorder((17.72±2.23) , (17.78±1.64)), emotional description disorder((13.44±1.94) , (13.41±1.79)), HADA((14.41±2.63) , (13.75±2.97)), anxiety ((7.03±1.64), (6.84±1.51)), depression ((7.38±1.45) , (6.91±1.75) ) at completed intervention and 1 month after intervention decreased ( P<0.05), while the HADS of control group only decreased at completed intervention ( P<0.05). Conclusion:SEGT can reduce alexithymia in lung cancer receiving chemotherapy, and this intervention has positive effect on improving anxiety and depression in lung cancer patients received chemotherapy who are accompanied with emotional cognitive impairment and emotional somatic symptom recognition disorder.