Objective:To investigate the relationship between methylation levels of ESR1, PRKN genes and the occurrence of polycystic ovary syndrome (PCOS). Methods:In a case-control study, 60 patients with PCOS (PCOS group) and 40 women of normal reproductive age (control group) attending the Department of Reproductive Medicine, the First Hospital of Shanxi Medical University from January 2021 to June 2021 were enrolled. The peripheral blood DNA and total RNA were extracted, and the methylation specific polymerase chain reaction (MS-PCR) technique was used to detect the methylation status of ESR1 and PRKN genes promoter regions. The mRNA expression levels of ESR1 and PRKN genes were measured by real-time quantitative PCR. The relationship between the methylation status, mRNA expression levels of these two genes and the occurrence of PCOS were analyzed. Results:The methylation level of ESR1 gene in PCOS patients [56.7% (34/60)] was significantly lower than that in control group [77.5% (31/40), P=0.035]; while the methylation level of PRKN gene in PCOS patients [76.7% (46/60)] was significantly higher than that in control group [52.5% (21/40), P=0.012]. The expression level of ESR1 mRNA in PCOS patients (1.30±0.93) was significantly higher than that in control group (0.97±0.50, P=0.034); while there was no difference of PRKN expression level between PCOS (1.06±0.83) and control groups (1.15±1.01, P>0.05). Conclusion:The methylation levels in the promoter regions of ESR1 and PRKN genes lead to the changes of the mRNA expression levels, which in turn play an important role in the occurrence of PCOS.

Objective:To investigate the relationship between methylation levels of ESR1, PRKN genes and the occurrence of polycystic ovary syndrome (PCOS). Methods:In a case-control study, 60 patients with PCOS (PCOS group) and 40 women of normal reproductive age (control group) attending the Department of Reproductive Medicine, the First Hospital of Shanxi Medical University from January 2021 to June 2021 were enrolled. The peripheral blood DNA and total RNA were extracted, and the methylation specific polymerase chain reaction (MS-PCR) technique was used to detect the methylation status of ESR1 and PRKN genes promoter regions. The mRNA expression levels of ESR1 and PRKN genes were measured by real-time quantitative PCR. The relationship between the methylation status, mRNA expression levels of these two genes and the occurrence of PCOS were analyzed. Results:The methylation level of ESR1 gene in PCOS patients [56.7% (34/60)] was significantly lower than that in control group [77.5% (31/40), P=0.035]; while the methylation level of PRKN gene in PCOS patients [76.7% (46/60)] was significantly higher than that in control group [52.5% (21/40), P=0.012]. The expression level of ESR1 mRNA in PCOS patients (1.30±0.93) was significantly higher than that in control group (0.97±0.50, P=0.034); while there was no difference of PRKN expression level between PCOS (1.06±0.83) and control groups (1.15±1.01, P>0.05). Conclusion:The methylation levels in the promoter regions of ESR1 and PRKN genes lead to the changes of the mRNA expression levels, which in turn play an important role in the occurrence of PCOS.

Objective     To evaluate the effect of intraoperative fluid infusion volume on postoperative pulmonary complications (PPCs) in patients after minimally invasive endoscopic esophageal carcinoma resection. Methods     From June 2019 to August 2021, 486 patients undergoing elective minimally invasive endoscopic esophagectomy for esophageal cancer were retrospectively screened from the electronic medical record information management system and anesthesia surgery clinical information system of West China Hospital of Sichuan University. There were 381 males and 105 females, with a median age of 64.0 years. Taking the incidence of pulmonary complications within 7 days after operation as the primary outcome, the correlation between intraoperative fluid infusion volume and the occurrence of PPCs within 7 days was clearly analyzed by regression analysis. Results    The incidence of pulmonary complications within 7 days after surgery was 33.5% (163/486). Regression analysis showed that intraoperative fluid infusion volume was correlated with the occurrence of PPCs [adjusted OR=1.089, 95%CI (1.012, 1.172), P=0.023], especially pulmonary infection [adjusted OR=1.093, 95%CI (1.014, 1.178), P=0.020], and pleural effusion [adjusted OR=1.147, 95%CI (1.007, 1.306), P=0.039]. Pulmonary infection was significantly less in the low intraoperative fluid infusion group [<6.49 mL/(kg·h), n=115] compared with the high intraoperative fluid infusion group [≥6.49 mL/(kg·h), n=371] (18.3% vs. 34.5%, P=0.023). Intraoperative fluid infusion volume was positively associated with death within 30 days after surgery [adjusted OR=1.442, 95%CI (1.056, 1.968), P=0.021]. Conclusion     Among patients undergoing elective minimally invasive endoscopic esophageal cancer resection, intraoperative fluid infusion volume is related with the occurrence of PPCs within 7 days after the surgery, especially pulmonary infection and pleural effusion, and may affect death within 30 days after the surgery.

Objective     To evaluate the association between pressure-controlled ventilation-volume guaranteed (PCV-VG) mode and volume-controlled ventilation (VCV) mode on postoperative pulmonary complications (PPCs) in patients undergoing thoracoscopic lung resection. Methods     A retrospective cohort analysis of 329 patients undergoing elective thoracoscopic lung resection in West China Hospital of Sichuan University between September 2020 and March 2021 was conducted, including 213 females and 116 males, aged 53.6±11.3 years. American Society of Anesthesiologists (ASA) grade wasⅠ-Ⅲ. The patients who received lung-protective ventilation strategy during anesthesia were divided into a PCV-VG group (n=165) and a VCV group (n=164) according to intraoperative ventilation mode. Primary outcome was the incidence of PPCs during hospitalization. Results     A total of 73 (22.2%) patients developed PPCs during hospitalization. The PPCs incidence of PCV-VG and VCV was 21.8% and 22.6%, respectively (RR=0.985, 95%CI 0.569-1.611, P=0.871). Multivariate logistic regression analysis showed that there was no statistical difference in the incidence of PPCs between PCV-VG and VCV mode during hospitalization (OR=0.846, 95%CI 0.487-1.470, P=0.553). Conclusion     Among patients undergoing thoracoscopic lung resection, intraoperative ventilation mode (PCV-VG or VCV) is not associated with the risk of PPCs during hospitalization.

Objective To evaluate biomechanical strength of locking compression plate (LCP) for fixation of periprosthetic proximal femur fractures (PPFF). Methods Eight matched pairs of Vancouver type B1 adult cadaveric PPFF specimens were fixed with the LCP and the inverted distal femoral less invasive stabilization system (LISS), respectively. Four bicortical locking screws (LCP group) and four unicortical locking screws were used to the length of prosthesis stem, and four double cortical locking screws were used to fix the distal end of the fracture in two groups, the distance from the locking screws to the fracture were also equal. The maximum bending load, maximum bending displacement, bending stiffness, maximum torque, maximum torsional angle and torsional stiffness of two groups in four-point bending test and torsion test were compared and analyzed. Results The maximum bending load, maximum bending displacement and bending stiffness of LCP group were all larger than those of LISS group, but the difference was not statistically significant (P>0.05). The maximum torque, maximum torsional angle and torsional stiffness of LCP group were obviously larger than those of LISS group，and there was a statistical difference between two groups (P<0.05). Conclusions The stiffness of anti-torsion with LCP is significantly better than that with LISS. Consequently, LCP has better biomechanical stability for PPFF.

Objective To explore the treatment of children with EB virus infection accompanied by facial paralysis. Method The clinical data of a child with EB virus infection accompanied by facial paralysis was analyzed retrospectively, and the related literature were reviewed. Results A 2-year-old boy was admitted to hospital due to fever and mouth askew for 4 days. After admission, he was confirmed to have EB virus infection and viremia by serology and polymerase chain reaction, and then treated with acyclovir. The symptoms of facial paralysis and EB viremia disappeared completely 14 days after antiviral treatment. There was no recurrence in the short-term follow-up. Interestingly, the literature analysis shows that there is still limited evidence for the antiviral treatment by acyclovir in children with acute infection of EB virus associated with facial paralysis. Conclusion Antiviral treatment may be beneficial to EB viremia with facial paralysis.

China ; Communicable Diseases ; Pediatrics ; Societies, Medical

Objective To observe the clinical effects and nursing therapy of Jinchuangkangjungao ointment and infrared therapy in the treatment of chronic skin ulcers .Methods Seventy-five patients with chronic skin ulcers were divided into two groups :the experimental group(n=39) and the control group(n=36) .Jinchuangkangjungao ointment and infrared therapy were applied to the experimental group and the control group underwent conventional treatment ,including debridement ,dressing change and iodine wet compressing .The two group were both given basic nursing therapy .The effect and healing time were observed after 30 days . Results The experimental group′s total effective rate(92 .31% ) was significantly better than that of the control group(69 .44% ) , with significant difference(P<0 .01);The healing time of the experimental group[(11 .98 ± 6 .05)d] was significantly shorten than the control group[(16 .96 ± 7 .13)d] ,with significant difference(P<0 .05) .Conclusion Jinchuangkangjungao ointment combined with infrared therapy had noticeable effect in the treatment of chronic skin ulcers .

ObjectiveTo understand the clinical epidemiology of enterovirus 71 (EV71) in children with hand,foot,and mouth disease (HFMD) in Shanghai during 2010 to 2011.Methods The demographic,etiological and clinical data of children with HFMD were analyzed retrospectively.EV71 was tested in stool samples by one-step quantitative reverse transcription-polymerase chain reaction (RT-PCR).The date were analyzed by Chi-square test.ResultsEV71 was detected in 820 (54.45％) of 1506 inpatients in 2010 and in 924 (59.84％) of 1544 inpatients in 2011,respectively.The detection rates in severe cases and uncomplicated cases were 86.31％ and 46.67％ (x2 =247.146,P＜0.01) in 2010 and 88.78％ and 48.75％ (x2 =201.664,P＜0.01) in 2011,respectively.The detection rate of EV71 was the highest (60％- 67 ％) during May and June when HFMD peaked.Among 1744 EV71-infected HFMD inpatients,the male-to-female ratio was 1.78 ∶ 1 ; the proportion of cases was the lowest in infant ＜6 months of age (0.46％,8/1744),and the highest in children 1 years of age (34.92％,609/1744); children aged 1-3 years accounted for 76.78％ (1339/1744);and migrant children accounted for 72.76 ％ (1269/1744).The demographic characteristics in severe cases were similar to those in general EV71-infected children.Nine severe cases of pulmonary edema/hemorrhage were all infected with EV71.Conclusions EV71 was a major pathogen causing the outbreaks of HFMD and severe complications in Shanghai in 2010 and 2011.Most severe cases and all critically severe cases were associated with EV71 infection.

OBJECTIVETo analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.

METHODRat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.

RESULTIn rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.

CONCLUSIONThe methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.

Animals ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; administration & dosage ; analysis ; metabolism ; Hedyotis ; chemistry ; Male ; Mass Spectrometry ; methods ; Rats ; Rats, Sprague-Dawley