To investigate the clinical significance of serum soluble programmed cell death ligand-1 (PD-L1) in adult patients with community-acquired pneumonia (CAP). A total of 44 CAP patients, 54 severe CAP patients and 30 healthy volunteers were recruited in this study. Serum soluble PD-L1 were detected. Univariate and multivariate regression analyses were used to assess the influence of multiple clinical variables on prognosis. Serum soluble PD-L1 level in severe CAP group was 98.20(57.94, 128.90) ng/L, which was significantly higher than that in the CAP group [59.32(33.55, 92.58) ng/L] and healthy controls [20.44(12.15, 36.20) ng/L] (all P<0.001). PD-L1 level was positively correlated with CRUB-65( r=0.481, P<0.001) and the pneumonia severity index (PSI) score ( r=0.442, P<0.001). Univariate regression analysis showed that CURB-65 ( HR=2.544, 95% CI 1.324-4.889, P=0.005), PSI score ( HR=1.036, 95% CI 1.012-1.061, P=0.004), soluble PD-L1( HR=1.013, 95% CI 1.001-1.026, P=0.041) were risk factors of mortality during hospitalization. Multivariate regression analysis suggested that PSI score ( HR=1.042, 95% CI 1.012-1.073, P=0.005), soluble PD-L1 ( HR=1.011, 95% CI 1.002-1.071, P=0.020) were independent predictors for mortality risk in CAP patients. CAP patients with soluble PD-L1≥98.20 ng/L had a significantly lower survival rate than those with soluble PD-L1<98.20 ng/L ( P=0.033). In conclusion, this study indicates that serum soluble PD-L1 level in CAP patients is correlated with the survival prognosis.

Objective:To explore the application of preimplantation genetic testing (PGT) in the treatment of infertile couples with abnormal chromosome structure, and provide a reference for the promotion and application of PGT technology.Methods:The clinical data of the first batch of patients who received PGT due to chromosomal structural abnormalities in Xinjiang JIAYIN Reproductive Medicine Center from June 2017 to July 2019 were retrospectively analyzed. According to the abnormal type, the patients were divided into three groups: mutual translocation, Roche translocation and inversion. After intracytoplasmic sperm injection (ICSI), the available blastocysts were selected for external trophoblast cell biopsy and next generation sequencing analysis of chromosome of embryonic cells. Embryos with normal detection results were selected for single blastocyst thawing and transplantation. The patient's general information, ovulation induction and embryo culture, blastocyst biopsy results and pregnancy outcome after transplantation were compared among the three groups. Results:A total of 51 patients completed 67 PGT cycles, a total of 189 blastocysts were genetically detected. There were significant differences among the three groups in the ratio of complex abnormal embryos and aneuploidy embryos (all P<0.001), but no significant differences were observed in other data ( P>0.05). There were 16 successful pregnancies out of 33 successful transplant cycles, with a pregnancy rate of 48.5%, and 7 patients had successfully given birth to healthy babies. Conclusion:The clinical application of PGT can effectively improve the fertility of couples with abnormal chromosome structure, and the proportion of embryo abnormalities of different types of carriers is different, which can be used as a genetic reference.

Objective:To explore the application of preimplantation genetic testing (PGT) in the treatment of infertile couples with abnormal chromosome structure, and provide a reference for the promotion and application of PGT technology.Methods:The clinical data of the first batch of patients who received PGT due to chromosomal structural abnormalities in Xinjiang JIAYIN Reproductive Medicine Center from June 2017 to July 2019 were retrospectively analyzed. According to the abnormal type, the patients were divided into three groups: mutual translocation, Roche translocation and inversion. After intracytoplasmic sperm injection (ICSI), the available blastocysts were selected for external trophoblast cell biopsy and next generation sequencing analysis of chromosome of embryonic cells. Embryos with normal detection results were selected for single blastocyst thawing and transplantation. The patient's general information, ovulation induction and embryo culture, blastocyst biopsy results and pregnancy outcome after transplantation were compared among the three groups. Results:A total of 51 patients completed 67 PGT cycles, a total of 189 blastocysts were genetically detected. There were significant differences among the three groups in the ratio of complex abnormal embryos and aneuploidy embryos (all P<0.001), but no significant differences were observed in other data ( P>0.05). There were 16 successful pregnancies out of 33 successful transplant cycles, with a pregnancy rate of 48.5%, and 7 patients had successfully given birth to healthy babies. Conclusion:The clinical application of PGT can effectively improve the fertility of couples with abnormal chromosome structure, and the proportion of embryo abnormalities of different types of carriers is different, which can be used as a genetic reference.