Mechanical pain is one of the most common causes of clinical pain, but there remains a lack of effective treatment for debilitating mechanical and chronic forms of neuropathic pain. Recently, neurotoxin GsMTx4, a selective mechanosensitive (MS) channel inhibitor, has been found to be effective, while the underlying mechanism remains elusive. Here, with multiple rodent pain models, we demonstrated that a GsMTx4-based 17-residue peptide, which we call P10581, was able to reduce mechanical hyperalgesia and neuropathic pain. The analgesic effects of P10581 can be as strong as morphine but is not toxic in animal models. The anti-hyperalgesic effect of the peptide was resistant to naloxone (an μ-opioid receptor antagonist) and showed no side effects of morphine, including tolerance, motor impairment, and conditioned place preference. Pharmacological inhibition of TRPV4 by P10581 in a heterogeneous expression system, combined with the use of Trpv4 knockout mice indicates that TRPV4 channels may act as the potential target for the analgesic effect of P10581. Our study identified a potential drug for curing mechanical pain and exposed its mechanism.

Objective To explore the effects of physiological deep-sea water(PDSW) on hyperthermal tolerance of Kunming (KM ) mice in the 45 .0 ℃ environment .Methods Deep-sea water from the south Chinese sea was processed ,and the metallic ele-ments dissolved in the DSW were analysed .The mice were randomly divided into 2 groups :the control group received tap water ;the experimental group treated with PDSW for 15 d .And then the mice were fed in the 45 .0 ℃ conditions .The survival time and histo-morphometric analyses of the brain ,lung ,heart ,liver and kidney were investigated .Results The survival time in PDSW-fed group was significantly longer than that of the control group (P< 0 .05) .Moreover ,histomorphometric analyses showed that PDSW could protect the brain ,lung ,heart ,liver and kidney of KM mice from the 45 .0 ℃ conditions .The results of western blot revealed that ex-pression of HSP72 of liver tissues for PDSW-fed group substantially increased ,when compared with the control mice(P< 0 .05) . Conclusion PDSW could improve hyperthermal tolerance of KM mice ,which maybe in the relation with expression of HSP72 pro-moted by PDSW .

Objective To investigate the effect of home nursing on perioperative life quality of patients with gynecologic oncology.Methods Totally 80 patients with with gynecologic oncolo-gy during perioperative period were divided into observation group and control group.All patients received appropriate surgical treatment and perioperative nursing and home nursing was given addi-tionally in observation group.The perioperative physiological,psychological stress level and the life quality were compared between two groups.Results Psychological health condition and life quality in observation group were better than those in control group while the physiological stress level was lower than in control group and all the differences were significant (P <0.01).Conclusion Home nursing showed a satisfactory effect on patients with gynecologic oncology in terms of improving the psychological health,reducing the degree of stress and improving the quality of life during periopera-tive period.

Objective To investigate the effect of home nursing on perioperative life quality of patients with gynecologic oncology.Methods Totally 80 patients with with gynecologic oncolo-gy during perioperative period were divided into observation group and control group.All patients received appropriate surgical treatment and perioperative nursing and home nursing was given addi-tionally in observation group.The perioperative physiological,psychological stress level and the life quality were compared between two groups.Results Psychological health condition and life quality in observation group were better than those in control group while the physiological stress level was lower than in control group and all the differences were significant (P <0.01).Conclusion Home nursing showed a satisfactory effect on patients with gynecologic oncology in terms of improving the psychological health,reducing the degree of stress and improving the quality of life during periopera-tive period.