OBJECTIVE: To compare the prevalence of chromosomal aneuploidies and pregnancy outcomes of D5 and D6 blastocysts subjected to preimplantation genetic testing for aneuploidy (PGT-A). METHODS: Clinical and laboratory data of 268 couples who underwent PGT-A at the Reproductive Center of the First Affiliated Hospital of Zhengzhou University from September 2018 to September 2020 were collected. The prevalence of chromosomal aneuploidies and pregnancy outcomes of D5/D6 biopsied blastocysts were compared. RESULTS: Compared with D6 blastocysts, the euploidy rate of D5 blastocysts was significantly higher (49.1% vs. 41.1%, P = 0.001 1), whilst their aneuploidy rate was significantly lower (50.9% vs. 58.9%, P = 0.001 1). The rate of numerical abnormalities of D6 blastocysts was significantly higher than that of D5 blastocysts (27.9% vs. 20.2%, P = 0.000 5). For patients under 35 years old, the euploidy rate of D5 blastocysts was significantly higher than that of D6 blastocysts (53.8% vs. 44.3%, P = 0.001), whilst the numerical abnormality rate was significantly lower (16.3% vs. 23.9%, P = 0.001). For both D5 and D6 blastocysts, the euploidy rates for patients <= 35 were significantly higher than those for > 35. The elder group had the lowest rates for aneuploidies and live births. Compared with those receiving D6 blastocysts transplantation, the pregnancy rate, implantation rate and live birth rate for those receiving thawed D5 blastocysts transplantation were significantly higher (60.2% vs.37.0%, P = 0.000 3; 59.1% vs.37.0%, P = 0.000 6; 47.7% vs. 28.3%, P = 0.002). CONCLUSION For patients undergoing PGT-A, the chromosomal euploidy rate for D5 blastocysts is higher than that for D6 blastocysts, and the clinical outcome of D5 blastocysts with normal signal is better than that of D6 blastocysts. Elder patients have a higher rate of aneuploidies.
Female ; Pregnancy ; Humans ; Aged ; Adult ; Pregnancy Outcome ; Aneuploidy ; Blastocyst ; Genetic Testing ; Laboratories

Objective:To explore the newborn birth weight and its influencing factors of polycystic ovary syndrome (PCOS) patients during fresh embryo transfer cycle.Methods:In this retrospective cohort study, the clinical data of 5087 patients with singleton live births who underwent in vitro fertilization and embryo transfer (IVF-ET) in the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2014 to December 2019 were analyzed. The patients were categorized into PCOS group ( n=1095) and non-PCOS group (referred to as control group, n=3992) according to the cause of infertility. The general conditions and birth weight of the two groups were compared, and logistic regression analysis was performed to identify the factors affecting birth weight. Results:For embryo transfer on the 3rd day (day 3, D3), the clinical pregnancy rate [69.0% (1248/1808)] and the implantation rate [49.0% (1742/3555)] in PCOS group were higher than those in control group [59.1% (5661/9572) and 42.0% (7577/18 040), all P<0.001], while the live birth rate [37.0% (670/1810)] was lower than that in control group [49.0% (4697/9585), P<0.001]. For blastocyst transfer on the 5th day (day 5, D5), the implantation rate [63.1% (500/793)] and the live birth rate [54.0% (425/787)] in PCOS group were higher than those of control group [59.0% (1066/1806), P=0.042; 48.0% (876/1825), P=0.013]. The birth weight of the infants [(3 459.76±527.11) g] and the proportion of overweight infants [14.35% (61/425)] in PCOS group of D5 blastocyst transfer were higher than those in control group [(3 391.61±521.38) g, P=0.028; 8.22% (72/876), P<0.001] and PCOS group of D3 embryo transfer [(3 389.24±555.06) g, P=0.018; 9.25% (62/670), P=0.009], but the proportion of low weight infants [2.35% (10/425)] was lower than that in control group of the D5 blastocyst transfer [4.91% (43/876), P=0.029] and PCOS group of D3 embryo transfer [4.78% (32/670), P=0.042]. Logistic regression analysis showed that the level of female body mass index (BMI) affects the birth weight of D5 blastocyst transfer ( OR=1.12, 95% CI=0.052-0.175, P<0.001). Developmental timing of transferred embryos affects birth weight in PCOS patients ( OR=1.52, 95% CI=0.019-0.819, P=0.040). Conclusion:For PCOS patients, choosing D5 blastocyst transfer can obtain a higher rate of live birth and a lower proportion of low birth weight infants. It is recommended that blastocyst transfer be performed for PCOS patients in fresh cycle.

Objective:To explore the newborn birth weight and its influencing factors of polycystic ovary syndrome (PCOS) patients during fresh embryo transfer cycle.Methods:In this retrospective cohort study, the clinical data of 5087 patients with singleton live births who underwent in vitro fertilization and embryo transfer (IVF-ET) in the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2014 to December 2019 were analyzed. The patients were categorized into PCOS group ( n=1095) and non-PCOS group (referred to as control group, n=3992) according to the cause of infertility. The general conditions and birth weight of the two groups were compared, and logistic regression analysis was performed to identify the factors affecting birth weight. Results:For embryo transfer on the 3rd day (day 3, D3), the clinical pregnancy rate [69.0% (1248/1808)] and the implantation rate [49.0% (1742/3555)] in PCOS group were higher than those in control group [59.1% (5661/9572) and 42.0% (7577/18 040), all P<0.001], while the live birth rate [37.0% (670/1810)] was lower than that in control group [49.0% (4697/9585), P<0.001]. For blastocyst transfer on the 5th day (day 5, D5), the implantation rate [63.1% (500/793)] and the live birth rate [54.0% (425/787)] in PCOS group were higher than those of control group [59.0% (1066/1806), P=0.042; 48.0% (876/1825), P=0.013]. The birth weight of the infants [(3 459.76±527.11) g] and the proportion of overweight infants [14.35% (61/425)] in PCOS group of D5 blastocyst transfer were higher than those in control group [(3 391.61±521.38) g, P=0.028; 8.22% (72/876), P<0.001] and PCOS group of D3 embryo transfer [(3 389.24±555.06) g, P=0.018; 9.25% (62/670), P=0.009], but the proportion of low weight infants [2.35% (10/425)] was lower than that in control group of the D5 blastocyst transfer [4.91% (43/876), P=0.029] and PCOS group of D3 embryo transfer [4.78% (32/670), P=0.042]. Logistic regression analysis showed that the level of female body mass index (BMI) affects the birth weight of D5 blastocyst transfer ( OR=1.12, 95% CI=0.052-0.175, P<0.001). Developmental timing of transferred embryos affects birth weight in PCOS patients ( OR=1.52, 95% CI=0.019-0.819, P=0.040). Conclusion:For PCOS patients, choosing D5 blastocyst transfer can obtain a higher rate of live birth and a lower proportion of low birth weight infants. It is recommended that blastocyst transfer be performed for PCOS patients in fresh cycle.

Objective To explore the effects of physiological deep-sea water(PDSW) on hyperthermal tolerance of Kunming (KM ) mice in the 45 .0 ℃ environment .Methods Deep-sea water from the south Chinese sea was processed ,and the metallic ele-ments dissolved in the DSW were analysed .The mice were randomly divided into 2 groups :the control group received tap water ;the experimental group treated with PDSW for 15 d .And then the mice were fed in the 45 .0 ℃ conditions .The survival time and histo-morphometric analyses of the brain ,lung ,heart ,liver and kidney were investigated .Results The survival time in PDSW-fed group was significantly longer than that of the control group (P< 0 .05) .Moreover ,histomorphometric analyses showed that PDSW could protect the brain ,lung ,heart ,liver and kidney of KM mice from the 45 .0 ℃ conditions .The results of western blot revealed that ex-pression of HSP72 of liver tissues for PDSW-fed group substantially increased ,when compared with the control mice(P< 0 .05) . Conclusion PDSW could improve hyperthermal tolerance of KM mice ,which maybe in the relation with expression of HSP72 pro-moted by PDSW .

OBJECTIVETo investigate the effect of rapamycin, an mTOR inhibitor, on learning and memory ability of mice with pilocarpine (PILO)-induced seizure.

METHODSOne hundred and sixty male adult ICR mice were randomly grouped as vehicle control (n=20), rapamycin control (n=20), PILO model (n=40), rapamycin pre-treatment (n=40) and rapamycin post-treatment (n=40). PILO model and rapamycin treatment groups were injected with PILO to induce temporal lobe seizure. Rapamycin was administrated for 3 days before or after seizure. Morris water maze, Y maze and open field were used for the assessment of learning and memory, and FJB and Timm staining were conducted to detect the neuronal cell death and mossy fiber sprouting, respectively.

RESULTSNo significant cell death was observed in the mice with PILO-induced seizure. The learning and memory were impaired in mice 7 to 10 days after PILO-induced seizure, which was evident by prolongation of avoiding latency (P<0.05), decrease in number of correct reaction (P<0.01) and number of crossing (P<0.05). Treatment with rapamycin both pre-and post- PILO injection reversed seizure-induced cognitive impairment. In addition, rapamycin inhibited the mossy fiber sprouting after seizure (P<0.001).

CONCLUSIONRapamycin improves learning and memory ability in ICR mice after PILO-induced seizure, and its mechanism needs to be further studied.

Animals ; Cell Death ; drug effects ; Disease Models, Animal ; Epilepsy ; chemically induced ; drug therapy ; Learning ; drug effects ; Memory ; drug effects ; Mice ; Mice, Inbred ICR ; Neurons ; drug effects ; pathology ; Pilocarpine ; toxicity ; Sirolimus ; pharmacology