Objective To explore the molecular mechanism of cerebroprotein hydrolysate-1(CH-1)in improving cognitive impairment of VD at animal level,and to determine the regulatory effect of CH-1 on Nrf2/ARE signaling pathway.Methods Thirty-six SD rats were randomly divided into sham operation group,VD group,low-and high-dose groups,with 9 rats in each group.VD model was established by bilateral common carotid artery ligation,and CH-1 was injected intraperitone-ally for 3 weeks.Morris water maze test and new object recognition test were performed to evalu-ate cognitive function.Hippocampal tissues was collected for immunohistochemistry/Western blot analysis.Results Compared with the sham operation group,the VD group exhibited significantly prolonged escape latency at 2-4 d of Morris water maze test,and up-regulated expression of ubiquitinated protein,LC3 Ⅱ/Ⅰ ratio,P65 and Beclin1 protein in the hippocampus,while down-regulated P62 expression(P＜0.05).Obviously shortened escape latency was observed in the high-dose group at 3-4 d and the low-dose group at 4 d than the VD group(P＜0.05).The resi-dence time in target quadrant,number of platform crossings,total exploration time of novel object recognition in the high-dose group and the total exploration time of novel object recognition in the low-dose group were significantly longer than those in VD group(P＜0.05).The expression levels of ubiquitinated,LC3 Ⅱ/Ⅰ ratio,P65 and Beclin1 were significantly lower in the low-dose group and high-dose group than the VD group(P＜0.05).The expression level of P62 protein in the VD group,low-and high-dose group were significantly increased in a dose-dependent manner(2.78±0.44,1.80±0.24 vs 3.67±0.34;2.37±0.26,1.53±0.09 vs 2.92±0.19;2.74±0.14,1.81±0.19 vs 3.93±0.50;2.28±0.17,1.72±0.17 vs 3.17±0.31,P＜0.05).Conclusion CH-1 can effectively improve the cognitive ability of VD rats and reduce the autophagy of hippocampal neurons.This therapeutic effect may be closely related to its enhancing activity of Nrf2/ARE signaling pathway.

Objective The study aimed to explore the factors influencing individual variations in the therapeutic efficacy of botulinum toxin type A(BoNT-A)in the treatment of benign essential blepharospasm(BEB).Methods A retrospectively analysis was performed on the general information of 40 BEB patients who received BoNT-A injections at our center between 2018 and 2023.The information included gender,age,education level,disease duration,number of injections,injection dose,severity of clinical symptoms,injection methods,and other relevant factors.All patients received both the pretarsal(PT)injection and the PT combining with preseptal(PS)injection(PT-PS).A multivariate linear regression analysis was used to statistically analyze the factors affecting the duration of therapeutic effect(DOT).Results Multiple regression analysis showed that gender,age,education level,clinical symptoms and injection method were closely related to the DOT[F(8,71)=4.372,P<0.001],with the injection method being the strongest predictor.Specifically,the mean DOT for the two injection methods was 136.00(123.00,156.50)days,with the PT-PS method significantly longer than the PT method[144.50(132.25,161.75)vs.125.00(114.00,145.25),P<0.001].Conclusion Gender,age,educational level,clinical symptoms,and injection method are predictive indicators for the DOT of BoNT-A in BEB patients.Specifically,DOT is positively correlated with age but negatively correlated with educational level and severity of clinical symptom.Additionally,the DOT is longer in male patients compared to female patients and in those treated with the PT-PS injection method compared to the PT injection method.

Objective The study aimed to explore the factors influencing individual variations in the therapeutic efficacy of botulinum toxin type A(BoNT-A)in the treatment of benign essential blepharospasm(BEB).Methods A retrospectively analysis was performed on the general information of 40 BEB patients who received BoNT-A injections at our center between 2018 and 2023.The information included gender,age,education level,disease duration,number of injections,injection dose,severity of clinical symptoms,injection methods,and other relevant factors.All patients received both the pretarsal(PT)injection and the PT combining with preseptal(PS)injection(PT-PS).A multivariate linear regression analysis was used to statistically analyze the factors affecting the duration of therapeutic effect(DOT).Results Multiple regression analysis showed that gender,age,education level,clinical symptoms and injection method were closely related to the DOT[F(8,71)=4.372,P<0.001],with the injection method being the strongest predictor.Specifically,the mean DOT for the two injection methods was 136.00(123.00,156.50)days,with the PT-PS method significantly longer than the PT method[144.50(132.25,161.75)vs.125.00(114.00,145.25),P<0.001].Conclusion Gender,age,educational level,clinical symptoms,and injection method are predictive indicators for the DOT of BoNT-A in BEB patients.Specifically,DOT is positively correlated with age but negatively correlated with educational level and severity of clinical symptom.Additionally,the DOT is longer in male patients compared to female patients and in those treated with the PT-PS injection method compared to the PT injection method.

Objective To explore the molecular mechanism of cerebroprotein hydrolysate-1(CH-1)in improving cognitive impairment of VD at animal level,and to determine the regulatory effect of CH-1 on Nrf2/ARE signaling pathway.Methods Thirty-six SD rats were randomly divided into sham operation group,VD group,low-and high-dose groups,with 9 rats in each group.VD model was established by bilateral common carotid artery ligation,and CH-1 was injected intraperitone-ally for 3 weeks.Morris water maze test and new object recognition test were performed to evalu-ate cognitive function.Hippocampal tissues was collected for immunohistochemistry/Western blot analysis.Results Compared with the sham operation group,the VD group exhibited significantly prolonged escape latency at 2-4 d of Morris water maze test,and up-regulated expression of ubiquitinated protein,LC3 Ⅱ/Ⅰ ratio,P65 and Beclin1 protein in the hippocampus,while down-regulated P62 expression(P＜0.05).Obviously shortened escape latency was observed in the high-dose group at 3-4 d and the low-dose group at 4 d than the VD group(P＜0.05).The resi-dence time in target quadrant,number of platform crossings,total exploration time of novel object recognition in the high-dose group and the total exploration time of novel object recognition in the low-dose group were significantly longer than those in VD group(P＜0.05).The expression levels of ubiquitinated,LC3 Ⅱ/Ⅰ ratio,P65 and Beclin1 were significantly lower in the low-dose group and high-dose group than the VD group(P＜0.05).The expression level of P62 protein in the VD group,low-and high-dose group were significantly increased in a dose-dependent manner(2.78±0.44,1.80±0.24 vs 3.67±0.34;2.37±0.26,1.53±0.09 vs 2.92±0.19;2.74±0.14,1.81±0.19 vs 3.93±0.50;2.28±0.17,1.72±0.17 vs 3.17±0.31,P＜0.05).Conclusion CH-1 can effectively improve the cognitive ability of VD rats and reduce the autophagy of hippocampal neurons.This therapeutic effect may be closely related to its enhancing activity of Nrf2/ARE signaling pathway.

Objective:To improve the operability and stability of the middle cerebral artery occlusion(MCAO)model,we established a novel method,external carotid artery ligation free(ECA-LF),for MCAO model and compared its advantages with the traditional Longa method and Koizumi method.Methods:A total of 100 male C57BL/6 mice were randomly divided into four groups:sham group,Koizumi group,Longa group,and ECA-LF group.The advantages and disadvantages of each method were evaluated in terms of surgical operation time,postoperative animal survival rate,postoperative animal survival rate,neurological functional score,and stability of infarct size.Results:The ECA-LF method spent less time than either the Longa or the Koizumi method during the course of making MCAO model.Com-pared with the Longa group,the ECA-LF group had a lower Garcia score,but was comparable to the Koizumi group.The survival rate of animals in the ECA-LF group was significantly higher than the other two groups 7 days after surgery.The Morris water maze(MWM)test showed that there was no significant difference between the ECA-LF group and oth-er two groups in learning and memory ability.The degree of cerebral edema and infarct size in the ECA-LF group were comparable to the other two groups.Conclusion:Compared with the traditional Longa and Koizumi methods,the ECA-LF method simplifies the surgical procedures,shortens the model-making time,and improves the survival rate of model animal with similar stability to the traditional methods.It has certain advantages in stroke experimental research.

Botulinum toxin(BoNT)is currently the first-line method for treating focal dystonia,which causes muscle paralysis by chemical denervation.Recent neuroimaging studies have found that BoNT treatment could alter neuroplasticity in the brain of patients with focal dystonia.However,the specific central nervous system mechanisms have not been fully elucidated.To this end,here we review the neuroimaging studies on BoNT treatment for dystonia from three aspects:functional magnetic resonance imaging,structural magnetic resonance imaging,and positron emission tomography imaging.It suggests that BoNT may improve the symptoms of dystonia patients by affecting functional connectivity,microstructure,and metabolic levels of the cortex,basal ganglia,thalamus,and cerebellum,etc.Therefore,this review will provide a theoretical reference for further exploring the mechanism and developing potential therapeutic targets of dystonia.

Objective:To improve the operability and stability of the middle cerebral artery occlusion(MCAO)model,we established a novel method,external carotid artery ligation free(ECA-LF),for MCAO model and compared its advantages with the traditional Longa method and Koizumi method.Methods:A total of 100 male C57BL/6 mice were randomly divided into four groups:sham group,Koizumi group,Longa group,and ECA-LF group.The advantages and disadvantages of each method were evaluated in terms of surgical operation time,postoperative animal survival rate,postoperative animal survival rate,neurological functional score,and stability of infarct size.Results:The ECA-LF method spent less time than either the Longa or the Koizumi method during the course of making MCAO model.Com-pared with the Longa group,the ECA-LF group had a lower Garcia score,but was comparable to the Koizumi group.The survival rate of animals in the ECA-LF group was significantly higher than the other two groups 7 days after surgery.The Morris water maze(MWM)test showed that there was no significant difference between the ECA-LF group and oth-er two groups in learning and memory ability.The degree of cerebral edema and infarct size in the ECA-LF group were comparable to the other two groups.Conclusion:Compared with the traditional Longa and Koizumi methods,the ECA-LF method simplifies the surgical procedures,shortens the model-making time,and improves the survival rate of model animal with similar stability to the traditional methods.It has certain advantages in stroke experimental research.

Objective? To explore the effects of aromatherapy on ameliorating nausea and emesis in orthopedic patients after surgery under general anesthesia. Methods? Totally 180 patients treated in the Department of Orthopedic Trauma, Suzhou Hospital of Traditional Chinese Medicine from November 2017 to July 2018 by convenient sampling, and the orthopedic patients receiving general anesthesia were divided into the treatment group (n=90) and the control group (n=90) according to the random serial generated by SPSS. Patients in the control group sniffed saline and received conventional nursing care, while patients in the treatment group received aromatherapy with essential oil and conventional nursing care. The incidence and severity of postoperative nausea and emesis was compared between the two groups. Results? The incidence rate of nausea and emesis in the treatment group within 24 hours post operation was 13.33%, lower than that in the control group, which was 51.11%, and there was statistically significant difference (χ2=29.406,P＜0.05). The number of patients showing nausea and emesis with different degrees in the treatment group within 24 hours post operation was Class 0 of 78 cases,8 cases of ClassⅠ, 4 of ClassⅡ and 0 of ClassⅢ,respectively. The number of patients showing nausea and emesis with different degrees in the control group within 24 hours post operation was Class 0 of 44 cases, 15 cases of Class Ⅰ, 27 of Class Ⅱ and 4 of Class Ⅲ,respectively. The severity of nausea and emesis in the treatment group was lower than that in the control group, and there was statistically significant difference (Z=-5.656,P＜ 0.05). Conclusions? Aromatherapy can effectively reduce the incidence rate and severity of nausea and emesis in orthopedic patients after surgery under general anesthesia.

Objective To evaluate the clinical effects of Cyclic Cryotherapy system on early symptoms after total knee arthroplasty (TKA). Methods The randomized controlled trial(RCT) articles on Cyclic Cryotherapy system after TKA were retrieved from Cochrane Library, JBI, PubMed, Web of Science, EBSCO, CNKI, Wanfang database and China Biology Medicine disc (CBMdisca), with the time limitation from the establishment of the database to 31st May 2018. Two researchers screened the literature according to the criteria of inclusion and exclusion, extracted the data, and used RevMan 5.3.3 software for statistical analysis. Results A total of 12 studies (976 cases) were included. The results of Meta analysis showed that, comparing between the Cyclic Cryotherapy and ice pack, the differences on pain score 24 hours after operation [MD=-0.70, 95%CI (-0.97, -0.43), P＜ 0.000 01], volume of drainage 48 hours after operation [MD=-143.04, 95%CI (-166.51, -119.57), P＜0.000 01]and swelling 1 week after operation [MD=-0.22, 95%CI(-0.32, -0.13), P＜0.000 01]were all statistically significant. Conclusions The existing evidence shows that compared with the ice pack, the Cyclic Cryotherapy system has the advantages of early postoperative analgesia, hemostasis, later detumescence, promoting recovery, improving comfort and no adverse reactions of cryotherapy.

OBJECTIVE:To probe the role of responsible pharmacist on irrational medical order intervention in Pharmacy Intra-venous Admixture Service (PIVAS),and to improve rational drug use in our hospital. METHODS:501 115 medical orders from PIVAS of our hospital during Jan.-Jun. 2012 were analyzed retrospectively. Irrational medical orders of 499 189 medical orders dur-ing Jul.-Dec. 2013 were classified and summarized after the establishment of responsible pharmacists system and pharmacist inter-vention. RESULTS:The number of irrational medical orders was 918(0.183 2%),and decreased to 448(0.089 7%)after interven-tion. Irrational medical orders of solvent selection reduced from 134(0.026 7%)to 69(0.013 8%);irrational medical orders of sol-vent volume reduced from 435(0.086 8%) to 206(0.041 3%);irrational medical orders of drug dosage reduced from 241(0.048 1%)to 117(0.023 4%);irrational medical orders of drug compatibility reduced from 51(0.010 2%)to 28(0.005 6%);irrational medical orders of dosing frequency reduced from 17(0.003 4%)to 2(0.000 4%). CONCLUSIONS:What responsible pharmacists intervened in the irrational doctor’s orders of intravenous drip was effective and feasible in PIVAS,improved rational drug use and guaranteed the safety of drug use.