Objective To discuss the application of the"rotating guidewire and correcting the filter recovery hook direction technique"("rotation-correction loop technique"for short),a technique invented by the authors in clinical practice,in the retrieval of complex inferior vena cava filter(IVCF),and to discuss its technical skills and advantages.Methods The clinical data of 417 patients carrying an IVCF,who were admitted to the Department of Vascular Surgery of Second Hospital of Shanxi Medical University of China to retrieve IVCF between January 2022 and December 2022,were retrospectively analyzed.Taking the time spent on the retrieval of IVCF and the intraoperative radiation dose as the evaluation indicators,the advantages and disadvantages of the standard filter retrieval technique,the"rotation-correction loop technique"and the other loop-assisted techniques were compared.Results Both the intraoperative radiation dose and the time spent on the retrieval of IVCF using"rotation-correction loop technique"were remarkably lower than those of other loop-assisted techniques(P＜0.000 1).Conclusion For the retrieval of complex IVCF,especially for the IVCF which is heavily tilted and/or its recovered hook is attached to the vascular wall,the use of"rotation-correction loop technique"can shorten the time spent on the the retrieval of IVCF and reduce the intraoperative radiation dose.This technique carries high safety and practicability,the device is simple and it can be manipulated by single physician,which is conducive to clinical application and promotion.(J Intervent Radiol,2024,33:289-294)

Objective Our previous studies established that microRNA (miR)-451 from human umbilical cord mesenchymal stem cell-derived exosomes (hUC-MSC-Exos) alleviates acute lung injury (ALI). This study aims to elucidate the mechanisms by which miR-451 in hUC-MSC-Exos reduces ALI by modulating macrophage autophagy. Methods Exosomes were isolated from hUC-MSCs. Severe burn-induced ALI rat models were treated with hUC-MSC-Exos carrying the miR-451 inhibitor. Hematoxylin-eosin staining evaluated inflammatory injury. Enzyme-linked immunosorbnent assay measured lipopolysaccharide (LPS),tumor necrosis factor-α,and interleukin-1β levels. qRT-PCR detected miR-451 and tuberous sclerosis complex 1 (TSC1) expressions. The regulatory role of miR-451 on TSC1 was determined using a dual-luciferase reporter system. Western blotting determined TSC1 and proteins related to the mammalian target of rapamycin (mTOR) pathway and autophagy. Immunofluorescence analysis was conducted to examine exosomes phagocytosis in alveolar macrophages and autophagy level. Results hUC-MSC-Exos with miR-451 inhibitor reduced burn-induced ALI and promoted macrophage autophagy. MiR-451 could be transferred from hUC-MSCs to alveolar macrophages via exosomes and directly targeted TSC1. Inhibiting miR-451 in hUC-MSC-Exos elevated TSC1 expression and inactivated the mTOR pathway in alveolar macrophages. Silencing TSC1 activated mTOR signaling and inhibited autophagy,while TSC1 knockdown reversed the autophagy from the miR-451 inhibitor-induced. Conclusion miR-451 from hUC-MSC exosomes improves ALI by suppressing alveolar macrophage autophagy through modulation of the TSC1/mTOR pathway,providing a potential therapeutic strategy for ALI.

【Objective】 To establish a stable human megakaryocytic cell line with low expression of Le^y antigen to further study the role of Le^y on activation of platelets. 【Methods】 The expression level of the Le^y antigen in a human megakaryocytic cell line, DAMI, was determined using Western Blot and flow cytometry. The expression level of genes that encode fucosyltransferase (FUTs), which was involved in the biosynthesis of Le^y antigen, was also determined to identify the candidate genes to be knocked out. The candidate FUT gene was knocked out via a CRISPR/Cas 9 gene knockout system and cells with low Le^y antigen expression were sorted by flow cytometry. The sorted cell line was cultured and characterized. 【Results】 The Le^y was expressed intensively on DAMI cell. FUT1 and FUT4 mRNA was expressed relatively higher, both may be key enzymes for the biosynthesis of the Le^y antigen. In the DAMI cell line with the knockout of FUT1 gene, the expression of the Le^y adntigen was remarkedly reduced, while cell proliferation was not affected compared to the wildtype control cells. 【Conclusions】 Since various FUTs contributes to the biosynthesis of the Le^y antigen, the knockout of the primary one of them cannot totally block its biosynthesis, but only reduce its expression. In this study, a stable FUT gene knockout human megakaryocyticcell line is established using CRISPR/Cas 9 technology, which provides basis for the study of the impact of the Le^y antigen on platelet functions.

Objective : To investigate the effect and mechanism of prenatal hypoxia on pulmonary artery vascular function in adult male offspring. Methods : Sprague⁃Dawley pregnant rats were randomly divided into hypoxia group and control group. Pregnant rats in the hypoxia group were housed in the hypoxia chamber ( a mixture of 89. 5% nitrogen gas and 10. 5% oxygen) from gestational day 10th to 20th. Pregnant rats in the control group were fed under normal oxygen. All animals were fed with standard rat food and tap water, and allowed to give birth naturally. After weaning , the male offspring were raised to 4 months old. Male offspring rats were anesthetized with chloral hydrate , and pulmonary artery was isolated for vascular function and molecular experiments. Results : Compared with the control group , phenylephrine ( PE) Ⅳ induced contraction response in prenatal hypoxic pulmonary artery was decreased (P < 0. 001) . There was no difference in acetyl choline (ACH) Ⅳand sodium nitrate (SNP) Ⅳinduced diastolic responses between the two groups. Compared with control group , Cav1 . 2 protein (P < 0. 01) and mRNA levels (P < 0. 001) in hypoxia group were reduced. Conclusion Prenatal hypoxia can lead to a decreased PE⁃induced vasoconstriction response in adult male offspring , which is related to the down⁃regulation of Cav1. 2 expression in pulmonary artery.

Objective:To translate the English version of Sexual Interest and Desire Inventory-Female (SIDI-F) into Chinese, evaluate its reliability, validity and the proper cut-off point of diagnosis of hypoactive sexual desire disorder (HSDD) in China.Methods:Chinese version of SIDI-F was developed and 96 healthy women from January 1, 2019 to December 31, 2019 in Taihe Hospital, Shiyan City, Hubei Province were selected to fill in the Chinese version of SIDI-F and the Female Sexual Function Index (FSFI). Next, analyzed the reliability, validity and the cut-off point of diagnosis of HSDD of the SIDI-F.Results:The Cronbach coefficient of the Chinese version of SIDI-F was 0.931, split-half reliability was 0.922, the intra-group correlation coefficient was 0.805. Analysis of content validity of the SIDI-F indicated that the average of scale-level content validity index was 1.00, the item-level content validity index was 1.00, and the Pearson correlation coefficient between the score of SIDI-F and the erotica score of the FSFI (FSFI-D) was 0.802. Factor analysis of the Chinese version of SIDI-F showed good construct validity. The area under ROC was 0.835. With the SIDI-F score and the best cut-off point of 26.5, Youden index was the largest, at 0.635. The validity indicators were 76.7% for sensitivity, 86.8% for specificity, 5.95 for positive likelihood ratio.Conclusions:The Chinese version of SIDI-F has high reliability and validity in Chinese population, and these show 26.5 point can be used as the best cut-off value of diagnose HSDD.

【Objective】 Toexplore the viability of preparing washed RBCs ordeglycerolized RBCs(referred as frozen RBCs)from blood containing irregular antibodies, so as to provide references for formulating processing procedures of blood containing irregular antibodiestosave blood resources and reduce blood wastage. 【Methods】 Once irregular antibodies were yielded, their type and titerwere determined.The plasma was discarded, and the suspended RBCs were prepared and processed according to the standardized washing scheme.The processing effect of blood with different titers and types of antibodies was observed, and theirfurther clinical application was followed up. 【Results】 From May 2017 to July 2019, a total of 45 blood samples containing irregular antibodies were screened in our center. The overall qualified rate reached 91.1% after processing. 100%(25/25) was qualified in IgM, and 80.0%(16/20)in IgG.4samples, initially noncom for mingdue to IgG-Dwith titerranged8~16, met the requirements after one or two additional washing processes. Among them, 23 cases were issued and appliedin clinicaland noadeverse reactions to blood transfusionoccurred. 【Conclusion】 After appropriate processing, blood containing irregular antibody can be applied to the clinical to save blood resources. When the irregular antibody is IgG-D and the titer is high, the washing times should be increased, and only when there is no residual antibodycan the samples be issued to the clinical.

Objective:To investigate the clinical application of salvianolate for injection in Tongde Hospital of Zhejiang Province and evaluate its rationality of injection.Methods:From March 2018 to November 2018, 560 patients treated with salvianolate for injection in Tongde Hospital of Zhejiang Province were selected by random sampling method.The indications, usage and dosage, route of administration, choice of vehicle, concentration of vehicle, course of treatment, compatibility, contraindications and adverse reactions of salvianolate for injection were analyzed retrospectively.Results:Totally 560 inpatients received salvianolate for injection were collected, about 26.96%(151/560) was consistent with the indication, 99.82%(559/560) with a correct administration dosage and frequency, 100.00%(560/560) with right administration route, 95.00%(532/560) with a right solvent selection, 67.86%(380/560) with correct solvent concentration, 61.07%(342/560) with a correct treatment course, 100.00%(560/560) with a right medicine combination, about 94.46%(529/560) was consistent with coagulation function contraindication, about 90.18%(505/560) was consistent with liver function contraindication.The incidence of adverse reactions was 1.61%(9/560).Conclusion:In the clinical application of salvianolate for injection, a high rate of unreasonable usage is found in indication, treatment course and solvent selection.Because of the serious problems in the use of salvianolate injection, it should be used strictly to ensure clinical medication safety.

Objective:The mechanical properties of costal cartilage of different genders and layers were tested, and the differences in mechanical properties of costal cartilage of different genders and layers were discussed. The theoretical basis was provided for the clinical use of costal cartilage of different layers in different situations, and the long-term effect after cartilage implantation could be predicted.Methods:According to the inclusion criteria, from June 2018 to December 2019, patients who receive dauricle reconstruction with costal cartilage or supporting treatment with costal cartilage were collected from the Inpatient and Outpatient Department of Plastic Surgery of Sichuan Provincial People’s Hospital. The remaining central and marginal layers of the seventh costal cartilage were made into different sizes of cartilage blocks. The tensile strength test and compression test (including creep test and stress relaxation test) were carried out respectively. The counting data were described by the number of cases, and Fisher exact test was used to analyze the differences between groups; the measurement data were expressed by mean ± standard deviation, and the comparison between groups was analyzed witht-test.Results:A total of 28 patients, including 16 males, aged 12-24 years old and 12 females, aged 12-18 years old, were collected. In the tensile strength test, the tensile fracture rate of the central layer of costal cartilage in male was 87.5% (14 / 16) and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). The tensile fracture rate of central layer of costal cartilage in female was 83.3% (10 / 12), and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). In the creep test, there was no significant difference between the central layer and the marginal layer between 0.5 min and 2 min in men ( t=-1.439, P= 0.171), and there was no significant difference between the central layer and the marginal layer in women ( t=-0.731, P=0.480). In the stress relaxation test, there was no significant difference between the central layer of costal cartilage and the marginal layer in male ( t=-2.053, P=0.058); in female, the stress relaxation in the central layer of costal cartilage was (0.006 ± 0.003)%, and that in the marginal layer was (0.011 ± 0.004)%, which was significantly higher than that in the central layer ( t=-3.342, P=0.007). Conclusions:The central layer of the costal cartilage in young people is worse than the marginal layer in tensile strength, and the calcification obviously weakens the tensile strength of the costal cartilage. Clinically, considering the calcification of the cartilage, the appropriate layer should be selected according to different parts and uses.

Objective:The mechanical properties of costal cartilage of different genders and layers were tested, and the differences in mechanical properties of costal cartilage of different genders and layers were discussed. The theoretical basis was provided for the clinical use of costal cartilage of different layers in different situations, and the long-term effect after cartilage implantation could be predicted.Methods:According to the inclusion criteria, from June 2018 to December 2019, patients who receive dauricle reconstruction with costal cartilage or supporting treatment with costal cartilage were collected from the Inpatient and Outpatient Department of Plastic Surgery of Sichuan Provincial People’s Hospital. The remaining central and marginal layers of the seventh costal cartilage were made into different sizes of cartilage blocks. The tensile strength test and compression test (including creep test and stress relaxation test) were carried out respectively. The counting data were described by the number of cases, and Fisher exact test was used to analyze the differences between groups; the measurement data were expressed by mean ± standard deviation, and the comparison between groups was analyzed witht-test.Results:A total of 28 patients, including 16 males, aged 12-24 years old and 12 females, aged 12-18 years old, were collected. In the tensile strength test, the tensile fracture rate of the central layer of costal cartilage in male was 87.5% (14 / 16) and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). The tensile fracture rate of central layer of costal cartilage in female was 83.3% (10 / 12), and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). In the creep test, there was no significant difference between the central layer and the marginal layer between 0.5 min and 2 min in men ( t=-1.439, P= 0.171), and there was no significant difference between the central layer and the marginal layer in women ( t=-0.731, P=0.480). In the stress relaxation test, there was no significant difference between the central layer of costal cartilage and the marginal layer in male ( t=-2.053, P=0.058); in female, the stress relaxation in the central layer of costal cartilage was (0.006 ± 0.003)%, and that in the marginal layer was (0.011 ± 0.004)%, which was significantly higher than that in the central layer ( t=-3.342, P=0.007). Conclusions:The central layer of the costal cartilage in young people is worse than the marginal layer in tensile strength, and the calcification obviously weakens the tensile strength of the costal cartilage. Clinically, considering the calcification of the cartilage, the appropriate layer should be selected according to different parts and uses.

Objective: To sum up the repair techniques of secondary alar deformity after the first phase reconstruction of alar defect. Methods: From January 2010 to December 2017, 17 patients with secondary alar deformity were included. Secondary alar deformities after the first stage reconstruction of alar defect included the abnormalities of alar groove line, absence of alar-cheek groove and the notch of alar rim. Z-plasty, V-Y advanced flaps, local skin flap and other technologies were introduced to repair details. Results: Infection was found in one case of the reconstruction of the alar-cheek groove and the wound was healed by treatment. There was no necrosis occurred in other flap. The shape of the alar was satisfactory and the anterior nostril was not narrow. Patients were followed up for 1 month to 1 year. The average follow-up time was 5 months. No recurrence of nasal alar tumor was found. The patients were satisfied with the appearance. Conclusions The symmetrical and satisfactory nasal shape can be obtained with local flaps such as Z-plasty, V-Y advancement of flaps for secondary alar deformities.