Objective:To explore the latent categories of nurses' perception of high-performance work systems (HPWS) through latent profile analysis and analyze the differences in characteristics and influencing factors among different categories.Methods:A convenience sampling method was used to select 3 450 clinical nurses from ClassⅡ、Ⅲ hospitals in 12 regions of China between June and July 2024. General information questionnaires, the Perceived High-Performance Work System Scale, and the Nurse Voice Behavior Scale were used for data collection. Latent profile analysis was conducted to analyze nurses' perception of HPWS, and multi-class logistic regression was used to examine the influencing factors for different categories.Results:A total of 3 450 questionnaires were collected, with 3 385 valid responses, yielding an effective response rate of 98.12%. Nurses' perception of HPWS had an average score of (70.46±12.21), which could be divided into three latent categories: low perception (17%, 559/3 385), moderate perception (42%, 1 433/3 385), and high perception (41%, 1 393/3 385). The multi-class logistic regression analysis showed that job nature, title, position, years of service, monthly income, health impact on work, work duration, and monthly night shifts were significant factors influencing nurses' perception of HPWS ( P<0.05) . Conclusions:There is heterogeneity in the nurses' perception of HPWS. Nursing managers should focus on nurses with low perception of HPWS and provide interventions and support based on the characteristics and influencing factors of each category to improve nurses' voice behaviors.

Objective:To systematically evaluate the risk factors for dysphagia among community-dwelling older adults.Methods:A comprehensive literature search was conducted in databases, including China National Knowledge Infrastructure, Wanfang Data, VIP, PubMed, Medline, Embase, CINAHL, PsycINFO, Web of Science, and the Cochrane Library, with the search ending on July 31, 2024. The quality of cross-sectional studies was assessed using the Agency for Healthcare Research and Quality (AHRQ) criteria, while cohort and case-control studies were evaluated using the Newcastle-Ottawa Scale (NOS). Meta-analysis was performed using Stata 18.Results:A total of 26 studies were included. The prevalence of dysphagia in community-dwelling older adults ranged from 7.9% to 63.7%. The following were identified as the risk factors for dysphagia ( P<0.05) : advanced age [ OR=1.17, 95% CI (1.08, 1.28) ], history of coughing or choking [ OR=7.25, 95% CI (3.66, 14.33) ], poor oral health [ OR=1.99, 95% CI (1.63, 2.43) ], impaired tongue-lip motor function [ OR=3.41, 95% CI (1.48, 7.85) ], reduced tongue pressure [ OR=1.63, 95% CI (1.16, 2.32) ], chewing dysfunction [ OR=2.18, 95% CI (1.57, 3.04) ], medication use history [ OR=1.22, 95% CI (1.02, 1.47) ], malnutrition [ OR=1.92, 95% CI (1.04, 3.56) ], history of the comorbid disease [ OR=4.18, 95% CI (2.61, 6.71) ], history of stroke [ OR=3.27, 95% CI (1.69, 6.33) ], depression [ OR=3.18, 95% CI (2.18, 4.65) ], frailty [ OR=1.43, 95% CI (1.12, 1.82) ], and male gender [ OR=2.91, 95% CI (1.70, 4.96) ] . Conclusions:Medical staff should pay close attention to the above-mentioned risk factors and develop targeted prevention strategies to reduce the incidence of dysphagia in community-dwelling older adults.

Objective:To explore the latent categories of nurses' perception of high-performance work systems (HPWS) through latent profile analysis and analyze the differences in characteristics and influencing factors among different categories.Methods:A convenience sampling method was used to select 3 450 clinical nurses from ClassⅡ、Ⅲ hospitals in 12 regions of China between June and July 2024. General information questionnaires, the Perceived High-Performance Work System Scale, and the Nurse Voice Behavior Scale were used for data collection. Latent profile analysis was conducted to analyze nurses' perception of HPWS, and multi-class logistic regression was used to examine the influencing factors for different categories.Results:A total of 3 450 questionnaires were collected, with 3 385 valid responses, yielding an effective response rate of 98.12%. Nurses' perception of HPWS had an average score of (70.46±12.21), which could be divided into three latent categories: low perception (17%, 559/3 385), moderate perception (42%, 1 433/3 385), and high perception (41%, 1 393/3 385). The multi-class logistic regression analysis showed that job nature, title, position, years of service, monthly income, health impact on work, work duration, and monthly night shifts were significant factors influencing nurses' perception of HPWS ( P<0.05) . Conclusions:There is heterogeneity in the nurses' perception of HPWS. Nursing managers should focus on nurses with low perception of HPWS and provide interventions and support based on the characteristics and influencing factors of each category to improve nurses' voice behaviors.

Objective:To systematically evaluate the risk factors for dysphagia among community-dwelling older adults.Methods:A comprehensive literature search was conducted in databases, including China National Knowledge Infrastructure, Wanfang Data, VIP, PubMed, Medline, Embase, CINAHL, PsycINFO, Web of Science, and the Cochrane Library, with the search ending on July 31, 2024. The quality of cross-sectional studies was assessed using the Agency for Healthcare Research and Quality (AHRQ) criteria, while cohort and case-control studies were evaluated using the Newcastle-Ottawa Scale (NOS). Meta-analysis was performed using Stata 18.Results:A total of 26 studies were included. The prevalence of dysphagia in community-dwelling older adults ranged from 7.9% to 63.7%. The following were identified as the risk factors for dysphagia ( P<0.05) : advanced age [ OR=1.17, 95% CI (1.08, 1.28) ], history of coughing or choking [ OR=7.25, 95% CI (3.66, 14.33) ], poor oral health [ OR=1.99, 95% CI (1.63, 2.43) ], impaired tongue-lip motor function [ OR=3.41, 95% CI (1.48, 7.85) ], reduced tongue pressure [ OR=1.63, 95% CI (1.16, 2.32) ], chewing dysfunction [ OR=2.18, 95% CI (1.57, 3.04) ], medication use history [ OR=1.22, 95% CI (1.02, 1.47) ], malnutrition [ OR=1.92, 95% CI (1.04, 3.56) ], history of the comorbid disease [ OR=4.18, 95% CI (2.61, 6.71) ], history of stroke [ OR=3.27, 95% CI (1.69, 6.33) ], depression [ OR=3.18, 95% CI (2.18, 4.65) ], frailty [ OR=1.43, 95% CI (1.12, 1.82) ], and male gender [ OR=2.91, 95% CI (1.70, 4.96) ] . Conclusions:Medical staff should pay close attention to the above-mentioned risk factors and develop targeted prevention strategies to reduce the incidence of dysphagia in community-dwelling older adults.

Objective:To analyze the status quo and advanced trend of voice training research in the world, and provide new ideas for voice training research in China.Methods:The literature on voice training in Web of Science core collection database was searched from January 1, 2013 to July 31, 2023, and visual analysis was performed using CiteSpace 6.1.R6 software.Results:A total of 964 papers were retrieved, and the number of published papers showed an increasing trend. The country with the largest number of publications was the United States, with 387. The Journal with the most publications was Journal of Voice. The research focused on voice rehabilitation of patients with different voice disorders, voice training intervention methods, improvement of quality of life and improvement of training compliance. The application of voice training in geriatric diseases might be a future research trend. Conclusions:At present, the field of voice training is deepening in the direction of new research and application, and the public's demand for voice rehabilitation is increasing. It is suggested that multidisciplinary collaboration should be strengthened in the future to explore the clinical efficacy of voice training in more different populations and diseases.

Objective To evaluate the clinical application effect of a specific antibody detection kit for human echinococcosis (hydatidosis kit),and provide technical support for further optimization of the production process of hydatidosis kit.Methods Using the method of retrospective investigation,1 481 patients with hydatidosis and 1 055 no-hydatidosis patients who were diagnosed by operation,pathology (gold standard) and hydatidosis kit in First Affiliated Hospital of Xinjiang Medical University,from 2012 to 2016 were selected.The clinical data was analyzed.The diagnostic performance of the kit was evaluated.The stepwise discriminant analysis method was applied to construct discriminant analysis function and establish a diagnostic model for echinococcosis.The detection efficiency of four antigens in hydatidosis kit was analyzed.Results A total of 2 536 patients [1 275 males,1 261 females,aged (41.62 ± 18.43) years old] were investigated,with the highest proportion in the 30-59 age group (1 489cases).Liver was the main organ affected by echinococcosis.The sensitivity,specificity and consistency of the hydatidosis kit were 94.80％ (1 404/1 481),71.00％ (749/1 055),and 84.90％ (2 153/2 536),Yoden index was 0.66 and Kappa value was 0.68.The stepwise discriminant analysis function Y =0.777X1 + 0.258X2 + 0.241X3-1.575 was constructed by the stepwise discriminant analysis method.There was no significant difference between the consistency of stepwise discriminant analysis model and the current diagnostic criteria (85.73％ vs 84.90％,x2 =0.694,P ＞ 0.05).The consistency of differential diagnosis between vesicular and cystic echinococcosis was 76.07％ (1 068/1 404).There was no significant difference in the detection efficacy between Echinococcus granulosus cystic fluid antigen (EgCF) and hydatidosis kit (P ＞ 0.05).Conclusions The diagnosis and differential diagnosis efficiency of hydatidosis kit is high.It is suitable for clinical diagnosis and field epidemiological investigation of echinococcosis in hospitals at all levels.EgCF can be used as the antigen of echinococcosis monoantigen strip,and can be applied in the epidemiological investigation of echinococcosis.

Silver nanoparticles were prepared by chemical reduction. Fusarium graminearum was used as the test strain. To study the inhibition of F. graminearum by silver nanoparticles, we studied the activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and the contents of osmotic adjustment substances soluble protein, soluble sugar and malonaldehyde (MDA) in F. graminearum. Silver nanoparticles inhibited F. graminearum and the inhibitory effect was increased with the concentration of silver nanoparticles. The inhibition rate of 10 μg/mL silver nanoparticles was more than 90% and EC50 was 0.59 μg/mL. When the treating time prolonged (2, 4, 6, 8 and 10 h), the activity of SOD, CAT and POD increased firstly and then declined. SOD, POD and CAT reached the maximum at 4 hours, and decreased to minimum at 10 hours. Silver nanoparticles also increased the MDA content and reduced the soluble sugar and protein contents in pathogens. These results indicated that cell integrity was destroyed in the presence of silver. This may be one of the inhibiting mechanisms of silver nanoparticles on the growth of F. graminearum.

Objective To investigate the relationship between glucokinase regulator protein ( GCKR) gene polymorphism rs780094 and hyperuricemia in Uygur in Xinjiang. Methods A case-control study including 1 026 patients with hyperuricemia and 1 030 normal subjects was conducted. All the subjects were genotyped for GCKR gene rs780094 by Sequenom MassARRAY system. The results of rs780094 genotype and allele frequency between hyperuricemia group and control group were compared. The associations of different genotypes of rs780094 with blood pressure, blood lipid, and blood glucose were analyzed. Logistic regression analysis was used to analyse the relationship between polymorphism of rs780094 and hyperuricemia in Uygur in Xinjiang. Results The distributions of three genotypes(G/G, A/G, A/A) and two allele frequency (G and A) in GCKR rs780094 revealed statistical difference ( P<0. 05 ) between hyperuricemia group and control group. A tendency toward association with hyperuricemia was observed under dominant model(OR=1. 295, 95%CI 1. 078~1. 554,P=0. 006) and recessive model(OR=1. 284, 95% CI 1. 024 ~1. 611,P=0. 030). The levels of systolic blood pressure, diastolic blood pressure, and total cholesterol were lower in hyperuricemia group with GCKR gene rs780094 loci GG genotype than those with AA+AG genotype. After adjusting confounding factors which had significant difference in the single factor analysis, logistic regression analysis showed that rs780094 A/A and A/G might be risk factors of hyperuricemia in Uygur in Xinjiang (OR=1. 355,95% CI 1. 094 ~1. 679,P=0. 005). Conclusion The GCKR rs780094 is associated with hyperuricemia in Uygur in Xinjiang. The A/A and A/G genotype of the GCKR rs780094 may increase the risk of hyperuricemia.

Objective To understand the levels of calcium ,iron ,zinc and lead elements among children in Weihai area and their change rule in different age periods so as to provide a theoretical guidance for the prevention of related element deficiency and lead poisoning .Methods The levels of lead ,zinc ,calcium and iron were detected in 1 406 children going to our hospital were deected and the results were analyzed .Results The children′s calcium element level in Weihai area showed a declining trend with the age in‐crease ,while the Zn ,Fe and Pb elements levels demonstrated a upward trend .One hundred and three cases of hypocalcemia were de‐tected ,318 cases of low blood iron ,283 cases of low blood zinc and 11 cases of high blood lead were detected in this time .The blood calcium and lead levels had no statistically significance difference among different age groups (P>0 .05);the serum iron and serum zinc levels had statistical different between the 0-1 year old group and other age groups(P<0 .05) .Conclusion The distribution trend of trace elements levels among children in Weihai area is accord with general rule .The incidence rates of low blood zinc and low blood iron are higher .The children′s trace elements levels should be regularly detected .

OBJECTIVE:To study the effects of scorpion’s proteins with different molecular weights on angiogenesis of the transgenic zebrafish. METHODS:The vascular fluorescence transgenic zebrafish models were established. Scorpion’s proteins were separated by ultrafiltration and ion exchange chromatography to obtain the scorpion protein fractions with different molecular weights (3-10 ku,>10-50 ku and>50 ku). The embryos of transgenic zebrafishes were cultured in the above 10,100 and 500 μg/ml scor-pion’s proteins. Intersegmental vessels of the transgenic zebrafishes were counted under the fluorescence microscope to optimize the most suitable scorpion’s protein molecular weight. The vessels were counted again with >50 ku scorpion protein component 1 and 2,so as to select suitable component.RESULTS:The >50 ku scorpion’s protein fraction component 1 with the mass concentration of 500 μg/ml had the highest inhibitory activity for the angiogenesis of the transgenic zebrafish,with inhibitory rate of 92.59%. CONCLUSIONS:Scorpion’s protein and its fractions have the activity of angiogenesis inhibition,which may be one of anti-cancer mechanisms of scorpion.