Objective To explore the protective effect and mechanism of Xinkang Granules-containing serum in adriamycin-induced injury of cardiomyocytes H9C2 based on cGAS-STING signaling axis.Methods Adriamycin was used to induce the H9C2 cells injury model.The cells were divided into normal group,model group,Xinkang Granules group and inhibitor group.After 24 hours of intervention,the CCK-8 method was used to detect cell survival rate,the DCFH-DA fluorescent probe was used to detect the content of cell reactive oxygen species(ROS),cell apoptosis rate was detected by flow cytometry,ELISA was used to detect the content of tumor necrosis factor-α(TNF-α)in cell supernatant,colorimetry was used to detect lactate dehydrogenase(LDH)in cells,RT-qPCR was used to detect the expression of mitochondrial transcription factor A(TFAM),cyclic guanosine-adenylate synthetase(cGAS),stimulator of interferon genes(STING)and TNF-α mRNA,Western blot and immunofluorescence were used to detect the protein expressions of cGAS and STING.Results Compared with the normal group,cell survival rate in the model group was significantly reduced(P<0.01),the ROS content was significantly increased(P<0.01),the apoptosis rate significantly increased(P<0.01),the content of TNF-α in the supernatant significantly increased(P<0.01),the activity of LDH significantly increased(P<0.01),the expression of TFAM mRNA significantly decreased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expression of cGAS and STING significantly increased(P<0.01).Compared with the model group,cell survival rate in Xinkang Granules group and inhibitor group significantly increased(P<0.01),the ROS content significantly decreased(P<0.01),the apoptosis rate significantly decreased(P<0.01),the content of TNF-α in supernatant significantly decreased(P<0.01),the activity of LDH significantly decreased(P<0.01),the expression of TFAM mRNA significantly increased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expressions of cGAS and STING significantly decreased(P<0.05,P<0.01).Conclusion Xinkang Granules have a protective effect on adriamycin-induced H9C2 cardiomyocytes,which may be related to the inhibition of cGAS/STING axis activation and the secretion of inflammatory factors.

Objective:To investigate the impact of lymphovascular invasion (LVI) and perineural invasion (PNI) in pathological specimens on the prognosis of patients after radical prostatectomy (RP).Methods:A retrospective analysis was conducted on the clinical data of 766 patients who underwent RP in the First Affiliated Hospital of Zhengzhou University from June 2019 to June 2024. The median age was 68 (63, 72) years, and the median prostate specific antigen (PSA) value was 16.56 (8.35, 34.16) ng/ml. According to the biopsy Gleason score, 168 cases (21.9%) had a score of 6, 315 cases (41.1%) had a score of 7, and 283 cases (37.0%) had a score of ≥8. The relationships between LVI/PNI and clinicopathological factors such as biopsy/radical Gleason score, pathological stage, positive surgical margin, seminal vesicle invasion, and nodal involvement were analyzed. Recurrence-free survival (RFS) was compared between LVI-positive vs. LVI-negative and PNI-positive vs. PNI-negative groups using Kaplan-Meier survival curves. Univariate and multivariate Cox regression analyses were performed to identify risk factors for RFS and assess the impact of LVI and PNI on postoperative recurrence.Results:Among the 766 postoperative pathological results, radical Gleason scores were 6 in 113 cases (14.8%), 7 in 356 cases (46.5%), and ≥8 in 297 cases (38.7%). Pathological stages included T 2 in 571 cases (74.5%), T 3 in 177 cases (23.1%), and T 4 in 18 cases (2.3%). Positive surgical margins were observed in 240 cases (31.3%), seminal vesicle invasion in 147 cases (19.2%), and nodal involvement in 63 cases (8.2%). Postoperative adjuvant therapy was administered to 94 cases (12.3%). LVI was positive in 65 cases (8.5%) and negative in 701 cases (91.5%). Compared with the LVI-negative group, the LVI-positive group showed significant differences in radical Gleason scores [0 vs. 113 cases with score 6 (16.1%), 11 (16.9%) vs. 345 (49.2%) cases with score 7, 54 (83.1%) vs. 243 (34.7%) cases with score ≥8; χ2=59.782, P<0.01], positive surgical margins [42 (64.6%) vs. 198 (28.2%); χ2=36.572, P<0.01], seminal vesicle invasion [50 (76.9%) vs. 97 (13.8%); χ2=152.656, P<0.01], nodal involvement [23 (35.4%) vs. 40 (5.7%); χ2=69.414, P<0.01], pathological stages [T 2: 7 (10.8%) vs. 564 (80.5%), T 3: 51 (78.4%) vs. 126 (18.0%), T 4: 7 (10.8%) vs. 11 (1.5%); χ2=154.364, P<0.01], and adjuvant therapy [31 (47.7%) vs. 63 (9.0%); χ2=82.775, P<0.01]. PNI was positive in 339 cases (44.3%) and negative in 427 cases (55.7%). The PNI-positive group exhibited significant differences in radical Gleason scores [11 (3.2%) vs. 102 (23.9%) cases with score 6, 155 (45.7%) vs. 201 (47.1%) cases with score 7, 173 (51.1%) vs. 124 (29.0%) cases with score ≥8; χ2=78.234, P<0.01], positive surgical margins [170 (50.1%) vs. 70 (16.4%); χ2=100.072, P<0.01], seminal vesicle invasion [129 (38.1%) vs. 18 (4.2%); χ2=139.524, P<0.01], nodal involvement [44 (13.0%) vs. 19 (4.4%); χ2=18.215, P<0.01], pathological stages [T 2: 174 (51.3%) vs. 397 (93.0%), T 3: 147 (43.4%) vs. 30 (7.0%), T 4: 18 (5.3%) vs. 0; χ2=174.625, P<0.01], and adjuvant therapy [73 (21.5%) vs. 21 (4.9%); χ2=48.463, P<0.01]. During a median follow-up of 14 (6, 32) months, 140 cases (18.3%) experienced recurrence. The recurrence rates were significantly higher in LVI-positive vs. LVI-negative [27 (41.5%) vs. 113 (16.1%); χ2=25.731, P=0.006] and PNI-positive vs. PNI-negative groups [91 (26.8%) vs. 49 (11.5%); χ2=29.882, P<0.01]. Multivariate Cox regression analysis identified age ( HR=1.021, P<0.05), PSA level ( HR=1.002, P<0.05), biopsy Gleason score ( HR=2.020, P<0.05), and nodal involvement ( HR=2.625, P<0.05) as independent risk factors for recurrence, while adjuvant therapy was an independent protective factor ( HR=0.147, P<0.01). Radical Gleason score, pathological stage, positive surgical margin, seminal vesicle invasion, LVI and PNI were not independent risk factors for recurrence (all P>0.05). Conclusions:Patients with pathological LVI or PNI after RP exhibit higher radical Gleason scores, pathological stages, positive surgical margin rates, seminal vesicle invasion rates, and nodal involvement rates. LVI and PNI positivity predict shorter recurrence-free survival, but they do not serve as independent risk factors for postoperative recurrence after RP.

Objective To explore the protective effect and mechanism of Xinkang Granules-containing serum in adriamycin-induced injury of cardiomyocytes H9C2 based on cGAS-STING signaling axis.Methods Adriamycin was used to induce the H9C2 cells injury model.The cells were divided into normal group,model group,Xinkang Granules group and inhibitor group.After 24 hours of intervention,the CCK-8 method was used to detect cell survival rate,the DCFH-DA fluorescent probe was used to detect the content of cell reactive oxygen species(ROS),cell apoptosis rate was detected by flow cytometry,ELISA was used to detect the content of tumor necrosis factor-α(TNF-α)in cell supernatant,colorimetry was used to detect lactate dehydrogenase(LDH)in cells,RT-qPCR was used to detect the expression of mitochondrial transcription factor A(TFAM),cyclic guanosine-adenylate synthetase(cGAS),stimulator of interferon genes(STING)and TNF-α mRNA,Western blot and immunofluorescence were used to detect the protein expressions of cGAS and STING.Results Compared with the normal group,cell survival rate in the model group was significantly reduced(P<0.01),the ROS content was significantly increased(P<0.01),the apoptosis rate significantly increased(P<0.01),the content of TNF-α in the supernatant significantly increased(P<0.01),the activity of LDH significantly increased(P<0.01),the expression of TFAM mRNA significantly decreased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expression of cGAS and STING significantly increased(P<0.01).Compared with the model group,cell survival rate in Xinkang Granules group and inhibitor group significantly increased(P<0.01),the ROS content significantly decreased(P<0.01),the apoptosis rate significantly decreased(P<0.01),the content of TNF-α in supernatant significantly decreased(P<0.01),the activity of LDH significantly decreased(P<0.01),the expression of TFAM mRNA significantly increased(P<0.01),and the expressions of TNF-α,cGAS,STING mRNA and the protein expressions of cGAS and STING significantly decreased(P<0.05,P<0.01).Conclusion Xinkang Granules have a protective effect on adriamycin-induced H9C2 cardiomyocytes,which may be related to the inhibition of cGAS/STING axis activation and the secretion of inflammatory factors.

Objective:To investigate the impact of lymphovascular invasion (LVI) and perineural invasion (PNI) in pathological specimens on the prognosis of patients after radical prostatectomy (RP).Methods:A retrospective analysis was conducted on the clinical data of 766 patients who underwent RP in the First Affiliated Hospital of Zhengzhou University from June 2019 to June 2024. The median age was 68 (63, 72) years, and the median prostate specific antigen (PSA) value was 16.56 (8.35, 34.16) ng/ml. According to the biopsy Gleason score, 168 cases (21.9%) had a score of 6, 315 cases (41.1%) had a score of 7, and 283 cases (37.0%) had a score of ≥8. The relationships between LVI/PNI and clinicopathological factors such as biopsy/radical Gleason score, pathological stage, positive surgical margin, seminal vesicle invasion, and nodal involvement were analyzed. Recurrence-free survival (RFS) was compared between LVI-positive vs. LVI-negative and PNI-positive vs. PNI-negative groups using Kaplan-Meier survival curves. Univariate and multivariate Cox regression analyses were performed to identify risk factors for RFS and assess the impact of LVI and PNI on postoperative recurrence.Results:Among the 766 postoperative pathological results, radical Gleason scores were 6 in 113 cases (14.8%), 7 in 356 cases (46.5%), and ≥8 in 297 cases (38.7%). Pathological stages included T 2 in 571 cases (74.5%), T 3 in 177 cases (23.1%), and T 4 in 18 cases (2.3%). Positive surgical margins were observed in 240 cases (31.3%), seminal vesicle invasion in 147 cases (19.2%), and nodal involvement in 63 cases (8.2%). Postoperative adjuvant therapy was administered to 94 cases (12.3%). LVI was positive in 65 cases (8.5%) and negative in 701 cases (91.5%). Compared with the LVI-negative group, the LVI-positive group showed significant differences in radical Gleason scores [0 vs. 113 cases with score 6 (16.1%), 11 (16.9%) vs. 345 (49.2%) cases with score 7, 54 (83.1%) vs. 243 (34.7%) cases with score ≥8; χ2=59.782, P<0.01], positive surgical margins [42 (64.6%) vs. 198 (28.2%); χ2=36.572, P<0.01], seminal vesicle invasion [50 (76.9%) vs. 97 (13.8%); χ2=152.656, P<0.01], nodal involvement [23 (35.4%) vs. 40 (5.7%); χ2=69.414, P<0.01], pathological stages [T 2: 7 (10.8%) vs. 564 (80.5%), T 3: 51 (78.4%) vs. 126 (18.0%), T 4: 7 (10.8%) vs. 11 (1.5%); χ2=154.364, P<0.01], and adjuvant therapy [31 (47.7%) vs. 63 (9.0%); χ2=82.775, P<0.01]. PNI was positive in 339 cases (44.3%) and negative in 427 cases (55.7%). The PNI-positive group exhibited significant differences in radical Gleason scores [11 (3.2%) vs. 102 (23.9%) cases with score 6, 155 (45.7%) vs. 201 (47.1%) cases with score 7, 173 (51.1%) vs. 124 (29.0%) cases with score ≥8; χ2=78.234, P<0.01], positive surgical margins [170 (50.1%) vs. 70 (16.4%); χ2=100.072, P<0.01], seminal vesicle invasion [129 (38.1%) vs. 18 (4.2%); χ2=139.524, P<0.01], nodal involvement [44 (13.0%) vs. 19 (4.4%); χ2=18.215, P<0.01], pathological stages [T 2: 174 (51.3%) vs. 397 (93.0%), T 3: 147 (43.4%) vs. 30 (7.0%), T 4: 18 (5.3%) vs. 0; χ2=174.625, P<0.01], and adjuvant therapy [73 (21.5%) vs. 21 (4.9%); χ2=48.463, P<0.01]. During a median follow-up of 14 (6, 32) months, 140 cases (18.3%) experienced recurrence. The recurrence rates were significantly higher in LVI-positive vs. LVI-negative [27 (41.5%) vs. 113 (16.1%); χ2=25.731, P=0.006] and PNI-positive vs. PNI-negative groups [91 (26.8%) vs. 49 (11.5%); χ2=29.882, P<0.01]. Multivariate Cox regression analysis identified age ( HR=1.021, P<0.05), PSA level ( HR=1.002, P<0.05), biopsy Gleason score ( HR=2.020, P<0.05), and nodal involvement ( HR=2.625, P<0.05) as independent risk factors for recurrence, while adjuvant therapy was an independent protective factor ( HR=0.147, P<0.01). Radical Gleason score, pathological stage, positive surgical margin, seminal vesicle invasion, LVI and PNI were not independent risk factors for recurrence (all P>0.05). Conclusions:Patients with pathological LVI or PNI after RP exhibit higher radical Gleason scores, pathological stages, positive surgical margin rates, seminal vesicle invasion rates, and nodal involvement rates. LVI and PNI positivity predict shorter recurrence-free survival, but they do not serve as independent risk factors for postoperative recurrence after RP.

Objective To explore the intervention effect and molecular mechanism of Xinkang Granules on inflammatory factors in rats with chronic heart failure based on cGAS/STING signaling pathway.Methods SD rats were randomly divided into normal group and modeling group.The chronic heart failure model was established by intraperitoneal injection of Doxorubicin Hydrochloride.After successfully modeling,the rats were further divided into model group,Valsartan group and Xinkang Granules group.The model group was treated with distilled water every day,the Valsartan group was treated with Valsartan solution every day,and the Xinkang Granules group was treated with Xinkang Granules every day,all given for 4 consecutive weeks.Echocardiography was used to detect cardiac function,the pathological changes of myocardium were detected by hematoxylin-eosin staining(HE),the ultrastructural changes of myocardium in each group were observed by transmission electron microscope,and the contents of interleukin-1β(IL-1β)and interleukin-6(IL-6)in serum were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA expression levels of mitochondrial transcription factor A(TFAM),cyclic guanosine monophosphate-adenylate synthase(cGAS),interferon-stimulated gene(STING)and IL-6 in myocardial tissue of rats in each group were detected by real-time fluorescence quantitative method(qPCR).The protein expressions of cGAS and STING in rat myocardial tissue were detected by immunohistochemical method.Results Compared with the blank group,the rats in the model group had significant inflammatory cell infiltration and inflammatory edema in myocardial tissue,their cardiac function was significantly reduced(P＜0.05,P＜0.01),and serum inflammatory factors were significantly increased(P＜0.01).The mRNA expression of TFAM in myocardial tissue was significantly reduced(P＜0.01),the mRNA expressions of IL-6,cGAS,and STING were significantly increased(P＜0.01),and the protein expressions of cGAS and STING in the myocardial tissue were significantly increased(P＜0.01).Compared with the model group,the cardiac function of the rats in the Xinkang Granules group was significantly improved(P＜0.05,P＜0.01),the inflammatory infiltration of myocardial cells was reduced,the expression of serum inflammatory factors was significantly reduced(P＜0.01),the mRNA expression of TFAM in myocardial tissue was significantly increased(P＜0.05),and the mRNA expressions of IL-6,cGAS,and STING were significantly decreased(P＜0.01),the protein expressions of cGAS and STING in myocardial tissue were significantly decreased(P＜0.01).Conclusion Xinkang Granules can reduce the expression of inflammatory factors and improve cardiac function in rats with chronic heart failure.Its mechanism may be related to inhibiting the cGAS/STING signaling pathway.

Objective: To investigate a mass incident of bromadiolone poisoning and analyze related clinical data. Methods: An investigation was performed for a mass incident of bromadiolone poisoning in a place in Shandong, China in December 2015, and related clinical data were analyzed and summarized. Results: This incident was a mass incident of bromadiolone poisoning caused by spreading poison. The poisoned patients had major clinical manifestations of bleeding and coagulation disorder and all of them were cured after comprehensive rescue, especially after intravenous drip of vitamin K1. Conclusion Bromadiolone poisoning can cause severe visceral hemorrhage and coagulation disorder, and intravenous drip of vitamin K1 has a good therapeutic effect.

OBJECTIVETo explore a new procedure of laparoscopic dual anastomosis for mid-low rectal cancer to reduce postoperative complications.

METHODSClinical data of 56 patients with mid-low rectal cancer undergoing laparoscopic rectal cancer resection(modified double-stapling technique, MDST, modification group) in the Department of General Surgery, the First Affiliated Hospital of Soochow University from February 2010 to June 2014 were compared with the data of 64 patients with mid-low rectal cancer (conventional double-stapling technique, DST, convention group) in the same period based on gender, age, tumor size, the distance from lower edge to the dentate line and tumor staging, etc. Patients in the modification group received operation as follows: (1) the rectum distal end was closed vertically instead of horizontally. (2) the anastomosis was conducted in an "end-corner" approach. (3) upper corner of the closed line in the distal end of rectum was removed. (4) the lower corner of closed line in the distal end of rectum was removed using vascular occlusion clamp method. (5) two T-shaped interchanges ("dangerous triangle") of stapled sutures formed after anastomosis were strengthened with absorbable suture. Patients in the convention group received laparoscopic dual anastomosis using conventional method: two corners and "dangerous triangles" were kept without any treatment. The clinical outcomes of two groups were analyzed retrospectively.

RESULTSThe intraoperational blood loss, postoperative drainage volume, postoperative anastomotic stoma bleeding, bowel function return and hospital stay were not significantly different between the two groups (all P>0.05). As compared to the convention group, the modification group had longer operation time [(211 ± 91) min vs. (174 ± 57) min, P<0.05], lower incidence of postoperative anastomotic leakage [1.8%(1/56) vs. 12.5% (8/64), P=0.030], lower tenesmus rate [3.6% (2/56) vs. 14.1% (9/64), P<0.05], less postoperative stoma re-creation [0 vs. 9.4% (6/64), P<0.05].

CONCLUSIONModified laparoscopic dual anastomosis for mid-low rectal cancer can significantly reduce the incidence of post-surgical complications such as anastomotic leakage.

Anastomosis, Surgical ; Anastomotic Leak ; Humans ; Laparoscopy ; Neoplasm Staging ; Operative Time ; Postoperative Complications ; Rectal Neoplasms ; surgery ; Retrospective Studies

Background and purpose:Anastomotic leakage and low anterior resection syndrome(LARS) are both common complications in dual-anastomosis for patients with low rectal cancer. The aim of this study was to observe and explore the signiifcance and role of vertical cutting of the closed distal rectum in dual-anastomosis for patients with low rectal cancer.Methods:A total number of 120 patients with mid-low rectal cancer who admitted to and completed laparoscopic rectal cancer resection in the Department of General Surgery, the First Afifliated Hospital of Soochow University from Feb. 2010 to Jun. 2014 were pair-matched into Groups A and B based on their gender,age, tumor size, the distance of lower edge to the dentate line and tumor staging, etc. For the 55 patients in Group A (observation group), the rectum distal end was closed vertically instead of horizontally while disposing “the ifrst intestinal anastomosis”, intestine-intestine anastomosis was conducted in an “end-corner” approach when dealing with “the second intestinal anastomosis”, upper corner (“dog ear”) of the closed line in the distal end of the rectum was removed, the lower corner (“dog ear”) of the closed line in the distal end of the rectum was removed using vascular occlusion clamp method, and the T-shaped interchanges (“dangerous triangle”) of stapled sutures formed after anastomosis were strengthened with absorbable suture. For the 65 patients in Group B (control group), laparoscopic dual anastomosis was conducted using conventional method, and the two “dog-ears” and “dangerous triangles” were kept without any treatment. The clinical outcomes of the two groups of patients were analyzed retrospectively. Results:In group A, It was convenient to complete the operation when the “dog ears” and “dangerous triangle” on the vertical line after cutting the closed distal rectum vertically by “end-corner” anastomosis. The axis of intestine formed a certain angle making the closed distal rectum into “ampulla” sample without “dog ears”. the “dangerous triangle” were strengthened with absorbable suture. In group B, The distal and proximal intestine located on the same axis after intestine-intestine anastomosis leaving two “dog ears” and a “dangerous triangle”. The general clinical data of patients in the two groups were comparable and not signiifcantly different (P>0.05). The two groups of patients showed no signiifcant differences in blood loss, postoperative drainage, postoperative anastomotic bleeding, anal exhaust time, and length of hospital stay (P>0.05). However, the operation time as well as the numbers of anastomotic ifstula occurrence, defecation, tenesmus and post-operation re-ostomy differed significantly (P<0.05).Conclusion:Vertical cutting of the closed distal rectum with dual anastomosis made the “new” intestine closer to the physiological bending and morphology of the rectum, meanwhile, it simpliifed the approach of removing “dog ear” and strengthening “dangerous triangle”, ifnally it signiifcantly reduced the incidence of post-surgical complications.

Objective To explore a new procedure of laparoscopic dual anastomosis for mid-low rectal cancer to reduce postoperative complications. Methods Clinical data of 56 patients with mid-low rectal cancer undergoing laparoscopic rectal cancer resection (modified double-stapling technique, MDST, modification group) in the Department of General Surgery, the First Affiliated Hospital of Soochow University from February 2010 to June 2014 were compared with the data of 64 patients with mid-low rectal cancer (conventional double-stapling technique, DST, convention group) in the same period based on gender, age, tumor size, the distance from lower edge to the dentate line and tumor staging, etc. Patients in the modification group received operation as follows: (1) the rectum distal end was closed vertically instead of horizontally. (2) the anastomosis was conducted in an “end-corner”approach. (3) upper corner of the closed line in the distal end of rectum was removed. (4) the lower corner of closed line in the distal end of rectum was removed using vascular occlusion clamp method. (5) two T-shaped interchanges (“dangerous triangle”) of stapled sutures formed after anastomosis were strengthened with absorbable suture. Patients in the convention group received laparoscopic dual anastomosis using conventional method: two corners and “dangerous triangles” were kept without any treatment. The clinical outcomes of two groups were analyzed retrospectively. Results The intraoperational blood loss, postoperative drainage volume, postoperative anastomotic stoma bleeding, bowel function return and hospital stay were not significantly different between the two groups (all P>0.05). As compared to the convention group, the modification group had longer operation time [(211± 91) min vs. (174±57) min, P<0.05], lower incidence of postoperative anastomotic leakage [1.8%(1/56) vs. 12.5%(8/64), P=0.030], lower tenesmus rate [3.6%(2/56) vs. 14.1%(9/64), P<0.05], less postoperative stoma re-creation [0 vs. 9.4%(6/64), P<0.05]. Conclusion Modified laparoscopic dual anastomosis for mid-low rectal cancer can significantly reduce the incidence of post-surgical complications such as anastomotic leakage.

Objective To explore a new procedure of laparoscopic dual anastomosis for mid-low rectal cancer to reduce postoperative complications. Methods Clinical data of 56 patients with mid-low rectal cancer undergoing laparoscopic rectal cancer resection (modified double-stapling technique, MDST, modification group) in the Department of General Surgery, the First Affiliated Hospital of Soochow University from February 2010 to June 2014 were compared with the data of 64 patients with mid-low rectal cancer (conventional double-stapling technique, DST, convention group) in the same period based on gender, age, tumor size, the distance from lower edge to the dentate line and tumor staging, etc. Patients in the modification group received operation as follows: (1) the rectum distal end was closed vertically instead of horizontally. (2) the anastomosis was conducted in an “end-corner”approach. (3) upper corner of the closed line in the distal end of rectum was removed. (4) the lower corner of closed line in the distal end of rectum was removed using vascular occlusion clamp method. (5) two T-shaped interchanges (“dangerous triangle”) of stapled sutures formed after anastomosis were strengthened with absorbable suture. Patients in the convention group received laparoscopic dual anastomosis using conventional method: two corners and “dangerous triangles” were kept without any treatment. The clinical outcomes of two groups were analyzed retrospectively. Results The intraoperational blood loss, postoperative drainage volume, postoperative anastomotic stoma bleeding, bowel function return and hospital stay were not significantly different between the two groups (all P>0.05). As compared to the convention group, the modification group had longer operation time [(211± 91) min vs. (174±57) min, P<0.05], lower incidence of postoperative anastomotic leakage [1.8%(1/56) vs. 12.5%(8/64), P=0.030], lower tenesmus rate [3.6%(2/56) vs. 14.1%(9/64), P<0.05], less postoperative stoma re-creation [0 vs. 9.4%(6/64), P<0.05]. Conclusion Modified laparoscopic dual anastomosis for mid-low rectal cancer can significantly reduce the incidence of post-surgical complications such as anastomotic leakage.