Objective To explore the expression changes of Galanin,Obestatin and Apelin-12 in the serum of Gastric Cancer(GC)patients and their correlation with Helicobacter pylori(Hp)infection.Methods 92 GC patients admitted to Tianmen First People's Hospital Affiliated to Wuhan University of Science and Technology from December 2021 to December 2023 were re-garded as the GC group,and a total of 76 age-and sex-matched non-GC(gastritis)patients were selected as control group.The 13C breath test method was used to detect Hp infection in patients.Basedon the test results,Hp infected GC patients were separat-ed into grade Ⅰ infection group(n=23),grade Ⅱ infection group(n=29)and grade Ⅲ infection group(n=26).Enzyme linked im-munosorbent assay(ELISA)was applied to detect serum levels of Galanin,Obestatin,Apelin-12 and inflammatory factors.Pear-son's method was used to analyze the correlation between serum levels of Galanin,Obestatin and Apelin-12 and Hp infection in GC patients.Receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value of serum Galanin,Obestatin and Apelin-12 for GC.Results The Hp positive rate in the GC group was greatly higher than that in the control group(39.47%vs 84.78%),and the differences was statistically significant(χ2=37.214,P<0.001).The serum Galanin level in GC group(19.69±7.23pg/ml)was lower than that in control group(32.73±12.45pg/ml),the level of Obestatin,apelin-12(40.46±11.78ng/ml,323.09±59.25ng/L)were higher those that of the control group(26.15±8.27ng/ml,264.72±40.96ng/l),and the differences were statistically significant(t=8.468,8.926,7.271,all P<0.05),and their levels were related to TNM staging and lymph node metastasis(t=2.963～12.070,all P<0.05).Comparison of serum Galanin,Obestatin and Apelin-12 levels between the non-infected group and theⅠ,ⅡandⅢ grade infection group showed statistically significant differences(F=26.594,38.245,13.545,all P<0.05).Pearson correnlation analysis showed that serum Galanin levels were negatively correlated with DOB values and TNF-α,IL-1β and IL-6 levels(r=-0.477～-0.387,all P<0.001),Obestatin and Apelin-12 levels were positively correlated with DOB values and TNF-α,IL-1β,IL-6 levels(r=0.389～0.453,all P<0.001).The AUC(95%CI)of serum Galanin,Obestatin and Apelin-12 levels for single and combined diagnosis of GC were 0.853(0.790～0.903),0.809(0.741～0.865),0.803(0.735～0.861)and 0.925(0.874～0.960),respectively.The combined diagnostic efficacy superior to the individual diagnosis of Galanin,Obestatin,and Apelin-12,and the differences were statistically significant(Z=2.237,3.719,3.913,all P<0.05).Conclusion The low expression of Galanin and high expression of Obestatin and Apelin-12 in serum of GC patients are closely related to Hp infection,and their combination has certain diagnostic value for GC.

Objective To explore the expression changes of Galanin,Obestatin and Apelin-12 in the serum of Gastric Cancer(GC)patients and their correlation with Helicobacter pylori(Hp)infection.Methods 92 GC patients admitted to Tianmen First People's Hospital Affiliated to Wuhan University of Science and Technology from December 2021 to December 2023 were re-garded as the GC group,and a total of 76 age-and sex-matched non-GC(gastritis)patients were selected as control group.The 13C breath test method was used to detect Hp infection in patients.Basedon the test results,Hp infected GC patients were separat-ed into grade Ⅰ infection group(n=23),grade Ⅱ infection group(n=29)and grade Ⅲ infection group(n=26).Enzyme linked im-munosorbent assay(ELISA)was applied to detect serum levels of Galanin,Obestatin,Apelin-12 and inflammatory factors.Pear-son's method was used to analyze the correlation between serum levels of Galanin,Obestatin and Apelin-12 and Hp infection in GC patients.Receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value of serum Galanin,Obestatin and Apelin-12 for GC.Results The Hp positive rate in the GC group was greatly higher than that in the control group(39.47%vs 84.78%),and the differences was statistically significant(χ2=37.214,P<0.001).The serum Galanin level in GC group(19.69±7.23pg/ml)was lower than that in control group(32.73±12.45pg/ml),the level of Obestatin,apelin-12(40.46±11.78ng/ml,323.09±59.25ng/L)were higher those that of the control group(26.15±8.27ng/ml,264.72±40.96ng/l),and the differences were statistically significant(t=8.468,8.926,7.271,all P<0.05),and their levels were related to TNM staging and lymph node metastasis(t=2.963～12.070,all P<0.05).Comparison of serum Galanin,Obestatin and Apelin-12 levels between the non-infected group and theⅠ,ⅡandⅢ grade infection group showed statistically significant differences(F=26.594,38.245,13.545,all P<0.05).Pearson correnlation analysis showed that serum Galanin levels were negatively correlated with DOB values and TNF-α,IL-1β and IL-6 levels(r=-0.477～-0.387,all P<0.001),Obestatin and Apelin-12 levels were positively correlated with DOB values and TNF-α,IL-1β,IL-6 levels(r=0.389～0.453,all P<0.001).The AUC(95%CI)of serum Galanin,Obestatin and Apelin-12 levels for single and combined diagnosis of GC were 0.853(0.790～0.903),0.809(0.741～0.865),0.803(0.735～0.861)and 0.925(0.874～0.960),respectively.The combined diagnostic efficacy superior to the individual diagnosis of Galanin,Obestatin,and Apelin-12,and the differences were statistically significant(Z=2.237,3.719,3.913,all P<0.05).Conclusion The low expression of Galanin and high expression of Obestatin and Apelin-12 in serum of GC patients are closely related to Hp infection,and their combination has certain diagnostic value for GC.