Objective To investigate the correlation between serum microRNA(miR)-410,miR-17-5p,and miR-21 expression levels with disease activity in patients with psoriasis vulgaris.Methods A total of 126 pa-tients with psoriasis vulgaris who visited Department of Dermatology in the hospital from January 2022 to January 2024 were included as the research group(51 cases in the active phase,37 cases in the quiescent phase,and 38 cases in the degenerative phase).According to the Psoriasis Area and Severity Index(PASI)scoring method,the patients were assigned into 52 cases of mild piasis group and 74 cases of moderate to se-vere sis group.126 healthy individuals who underwent physical examinations at the hospital were included as the control group.Fluorescence quantitative PCR was applied to detect the expression levels of miR-410,miR-17-5p,and miR-21.The general clinical data of the research group and the control group were compared.The expression levels of serum miR-410,miR-17-5p,and miR-21 were compared among different groups.Spearman method was applied to analyze the correlation between serum miR-410,miR-17-5p,miR-21 and disease activity(PASI score)in patients with psoriasis vulgaris.Receiver operating characteristic curve was applied to analyze the diagnostic value of serum miR-410,miR-17-5p,and miR-21 for disease activity in patients with psoriasis vulgaris.Results Compared with the control group,the serum miR-410 expression level in the research group was lower,while the serum miR-17-5p and miR-21 expression levels were higher(P＜0.05).The serum miR-410 expression level of patients with psoriasis vulgaris in different periods showed a decreasing trend and ser-um miR-17-5p and miR-21 expression levels showed increasing trends compared with the control group(P＜0.05).The serum miR-410 expression level in the moderate to severe group was lower than that in the mild group,while the serum miR-17-5p and miR-21 expression levels were higher than those in the mild group(P＜0.05).Serum miR-410 was negatively correlated with PASI score in patients with psoriasis vulgaris,while serum miR-17-5p and miR-21 were positively correlated with PASI score(P＜0.05).The area under the curve of serum miR-410,miR-17-5p,miR-21,and their combined diagnosis for disease activity in patients with psoriasis vulgaris was 0.790,0.843,0.795,and 0.969,respectively.The combination of the three was superior to their individual diagnosis.Conclusion The occurrence and development of psoriasis vulgaris are related to the expression levels of serum miR-410,miR-17-5p and miR-21,and the changes in the expression levels of three could evaluate the severity of the disease in patients with psoriasis vulgaris.

ObjectiveTo explore the impact of exogenous chitosan on the growth and metabolism of Glycyrrhiza uralensis Fisch. (G. uralensis) and to improve the quality of cultivated G. uralensis for both medicine and food and aid in the increase in the content of effective components in G. uralensis.MethodsIn this study, whole G. uralensis plants were treated with exogenous chitosan, and comprehensive analyses of secondary metabolites and proteins were conducted using liquid chromatography with tandem mass spectrometry and isobaric tag for relative and absolute quantitation, respectively. Effects of chitosan induction on endogenous hormones of G. uralensis were analyzed using an enzyme-linked immunosorbent assay. Gene ontology function annotation and Kyoto Encyclopedia of Genes and Genomes pathway annotation were conducted to study the effect of chitosan induction on the proteome.ResultsChitosan induction significantly increased the levels of flavonoids in G. uralensis; however, the variation in triterpenoids was not substantial. Biological processes, including photosynthesis, secondary metabolism, and abiotic stress responses, were significantly enriched. Additionally, the photosynthetic pathway, photosynthesis-antenna protein pathway, and plant hormone signal transduction pathway were significantly enriched. In the flavonoid biosynthesis pathway, the upstream-related enzyme phenylalanine ammonia-lyase (PAL) and the downstream-related enzymes chalcone synthase (CHS), polyketide reductase (PKR), chalcone isomerase (CHI), and vestitone reductase (VR) were significantly upregulated.ConclusionsOur findings suggest that chitosan induction may promote the tricarboxylic acid (TCA) cycle, and the TCA cycle enhancement significantly upregulated PAL, CHS, PKR, CHI, and VR, the five key enzymes involved in flavonoid synthesis of G. uralensis, indicating that chitosan induction activated the entire metabolic pathway associated with flavonoids in G. uralensis. Our findings provide a reference for improving the quality of cultivated G. uralensis from the perspective of pharmacodynamic components.

Metabolic abnormality in type 2 diabetes mellitus(T2DM)can cause damage to the central nervous system,leading to cognitive decline.Neurofilament light chain protein(NFL),as a blood marker of neuroaxonal injuries,is significantly associated with the onset of cognitive impairment and affected by the renal function.It can participate in the development of cognitive impairment in T2DM through inflammation,blood-brain barrier breakdown,interaction between microglia and neurons,and Tau protein phosphorylation.We re-viewed the mechanism of the occurrence and development of NFL-involved cognitive impairment and the correla-tion between NFL and renal function in T2DM,hoping to provide a basis for early diagnosis and treatment of cog-nitive impairment in T2DM patients.

Recurrent spontaneous abortion(RSA)is one of the most common complications in early pregnancy,affecting about 2%～5%of women of childbearing age,and its incidence is increasing year by year,which not only affects women's physical and mental health,but also causes a heavy economic burden to the society and the family.Mesenchymal stem cells(MSCs)are a class of non-hematopoietic adult multipotent stem cells derived from mesodermal mesenchymal cells of a variety of tissues.They have the characteristics of multi-directional differentiation and self-renewal,and can improve the fertility outcome of RSA patients,which has become a research hotspot in recent years.Therefore,this paper explores the roles and mechanisms of MSCs from different sources in improving pregnancy outcomes in RSA patients,and analyzes their research status and existing problems.

Starting from 2020, Air Force Medical University has deepened the reform of medical English teaching for the eight-year program. Based on analysis of students' situation and course survey, an IPMET medical English proficiency framework comprising information literacy, problem solving, medical knowledge, English proficiency, and teamwork has been constructed. The university has established an eight-year program school-based medical English course focusing on medical humanities reading, medical paper writing, and military medical English. Additionally, it has hosted activities such as medical English speaking workshops and medical English situational practice competitions. A mode of teaching reform in curriculum construction has been developed aiming to broaden the positioning perspective, clarify the ability framework, and optimize the construction path. All of this comprehensively improves the medical English ability of eight-year program students.

Constructing a multi-tiered healthcare security system can meet the diverse and individualized healthcare service demand of the general public.Public hospitals,as the main providers of healthcare services in China,effectively integrate with commercial health insurance,which can accelerate the implementation of high-quality healthcare services,promote innovative development,and optimize product coverage and operational efficiency for commercial health insurance,achieving a win-win situation.Taking Shanghai as the research object,it analyzes the supply and demand status of multi-tiered healthcare security in Shanghai based on population characteristics,economic level,healthcare resources,and policy environment.It identifies the difficulties in the current collaboration between public hospitals and commercial insurance.Finally,it proposes exploring the expansion of independent development space for specialized services,improving the level of coordination between commercial insurance institutions and public hospitals in health management services,promoting the management and sharing of data,strengthening the protection of innovative medicines and devices by commercial insurance,in order to promote the coordinated development of commercial insurance and public hospitals.

Constructing a multi-tiered healthcare security system can meet the diverse and individualized healthcare service demand of the general public.Public hospitals,as the main providers of healthcare services in China,effectively integrate with commercial health insurance,which can accelerate the implementation of high-quality healthcare services,promote innovative development,and optimize product coverage and operational efficiency for commercial health insurance,achieving a win-win situation.Taking Shanghai as the research object,it analyzes the supply and demand status of multi-tiered healthcare security in Shanghai based on population characteristics,economic level,healthcare resources,and policy environment.It identifies the difficulties in the current collaboration between public hospitals and commercial insurance.Finally,it proposes exploring the expansion of independent development space for specialized services,improving the level of coordination between commercial insurance institutions and public hospitals in health management services,promoting the management and sharing of data,strengthening the protection of innovative medicines and devices by commercial insurance,in order to promote the coordinated development of commercial insurance and public hospitals.

Constructing a multi-tiered healthcare security system can meet the diverse and individualized healthcare service demand of the general public.Public hospitals,as the main providers of healthcare services in China,effectively integrate with commercial health insurance,which can accelerate the implementation of high-quality healthcare services,promote innovative development,and optimize product coverage and operational efficiency for commercial health insurance,achieving a win-win situation.Taking Shanghai as the research object,it analyzes the supply and demand status of multi-tiered healthcare security in Shanghai based on population characteristics,economic level,healthcare resources,and policy environment.It identifies the difficulties in the current collaboration between public hospitals and commercial insurance.Finally,it proposes exploring the expansion of independent development space for specialized services,improving the level of coordination between commercial insurance institutions and public hospitals in health management services,promoting the management and sharing of data,strengthening the protection of innovative medicines and devices by commercial insurance,in order to promote the coordinated development of commercial insurance and public hospitals.

Constructing a multi-tiered healthcare security system can meet the diverse and individualized healthcare service demand of the general public.Public hospitals,as the main providers of healthcare services in China,effectively integrate with commercial health insurance,which can accelerate the implementation of high-quality healthcare services,promote innovative development,and optimize product coverage and operational efficiency for commercial health insurance,achieving a win-win situation.Taking Shanghai as the research object,it analyzes the supply and demand status of multi-tiered healthcare security in Shanghai based on population characteristics,economic level,healthcare resources,and policy environment.It identifies the difficulties in the current collaboration between public hospitals and commercial insurance.Finally,it proposes exploring the expansion of independent development space for specialized services,improving the level of coordination between commercial insurance institutions and public hospitals in health management services,promoting the management and sharing of data,strengthening the protection of innovative medicines and devices by commercial insurance,in order to promote the coordinated development of commercial insurance and public hospitals.

Constructing a multi-tiered healthcare security system can meet the diverse and individualized healthcare service demand of the general public.Public hospitals,as the main providers of healthcare services in China,effectively integrate with commercial health insurance,which can accelerate the implementation of high-quality healthcare services,promote innovative development,and optimize product coverage and operational efficiency for commercial health insurance,achieving a win-win situation.Taking Shanghai as the research object,it analyzes the supply and demand status of multi-tiered healthcare security in Shanghai based on population characteristics,economic level,healthcare resources,and policy environment.It identifies the difficulties in the current collaboration between public hospitals and commercial insurance.Finally,it proposes exploring the expansion of independent development space for specialized services,improving the level of coordination between commercial insurance institutions and public hospitals in health management services,promoting the management and sharing of data,strengthening the protection of innovative medicines and devices by commercial insurance,in order to promote the coordinated development of commercial insurance and public hospitals.