Objective:To construct a personalized service platform based on Internet of Things technique of medical equipment for rehabilitation information,so as to analyze the application effect of that in the management for rehabilitation information of patients.Methods:Based on the Internet of Things technique of medical equipment,a personalized service platform was constructed to conduct intelligent management for rehabilitation information of patients through sensor technique,wireless communication technique and big data analysis technique.A total of 112 patients who received treatment in the department of rehabilitation medicine of Dushan Hospital,Nanyang City Hospital of Traditional Chinese Medicine from February to December 2023 were selected,and they were divided into control group which adopted conventional platform to manage rehabilitation information,and observation group which adopted personalized service platform based on IoTs technique of medical equipment for rehabilitation information to manage rehabilitation information,with 56 cases in each group.A self-made questionnaire was used to investigate the satisfaction of patients of two groups for the service platform of rehabilitation information,and the acceptance scores of maintainers for platform,relevant operators,doctors and nurses for different service platform.The service efficiency and success of rehabilitation treatment of different service platforms were compared.Results:53 patients satisfied for the service platform of rehabilitation information(94.64%)in the observation group,which was higher than that in the control group,and the difference was statistically significant(x2=13.187,P＜0.05).The scores of convenience,flexibility and continuity of patients of observation group were respectively(94.74±3.36),(94.57±4.12)and(93.65±4.02)for the platform,which were higher than those of control group,and the differences were statistically significant(t=14.279,13.604,13.553,P＜0.05).The acceptance scores of maintainers for platform,relevant operators,doctors and nurses were respectively(91.69±2.11),(94.52±3.65),(93.87±4.58)and(94.74±3.36)for the personalized service platform for rehabilitation information of the observation group,which were higher than those in the control group,and the differences were statistically significant(t=15.280,13.236,12.373,14.279,P＜0.05).The waiting time,appointment time and treatment cycle of patients in observation group were respectively(4.65±2.01)min,(3.21±1.03)d and(13.02±1.02)months,which were shorter than those of control group,and the differences were statistically significant(t=9.768,11.401,12.300,P＜0.05).Conclusion:The personalized service platform based on the IoTs technique of medical equipment for rehabilitation information can take doctors to timely adjust rehabilitation programs by combining the dynamic changes of patients'information,and formulate targeted rehabilitation guidance,and provide effective rehabilitation treatment for patients,and reduce waiting time of patient,and shorten the rehabilitation cycle.

Digital technologies have become an integral part of complete denture restoration. With advancement in computer-aided design and computer-aided manufacturing (CAD/CAM), tools such as intraoral scanning, facial scanning, 3D printing, and numerical control machining are reshaping the workflow of complete denture restoration. Unlike conventional methods that rely heavily on clinical experience and manual techniques, digital technologies offer greater precision, predictability, and efficacy. They also streamline the process by reducing the number of patient visits and improving overall comfort. Despite these improvements, the clinical application of digital complete denture restoration still faces challenges that require further standardization. The major issues include appropriate case selection, establishing consistent digital workflows, and evaluating long-term outcomes. To address these challenges and provide clinical guidance for practitioners, this expert consensus outlines the principles, advantages, and limitations of digital complete denture technology. The aim of this review was to offer practical recommendations on indications, clinical procedures and precautions, evaluation metrics, and outcome assessment to support digital restoration of complete denture in clinical practice.
Humans ; Denture, Complete ; Computer-Aided Design ; Denture Design/methods* ; Consensus ; Printing, Three-Dimensional

Objective:To construct a personalized service platform based on Internet of Things technique of medical equipment for rehabilitation information,so as to analyze the application effect of that in the management for rehabilitation information of patients.Methods:Based on the Internet of Things technique of medical equipment,a personalized service platform was constructed to conduct intelligent management for rehabilitation information of patients through sensor technique,wireless communication technique and big data analysis technique.A total of 112 patients who received treatment in the department of rehabilitation medicine of Dushan Hospital,Nanyang City Hospital of Traditional Chinese Medicine from February to December 2023 were selected,and they were divided into control group which adopted conventional platform to manage rehabilitation information,and observation group which adopted personalized service platform based on IoTs technique of medical equipment for rehabilitation information to manage rehabilitation information,with 56 cases in each group.A self-made questionnaire was used to investigate the satisfaction of patients of two groups for the service platform of rehabilitation information,and the acceptance scores of maintainers for platform,relevant operators,doctors and nurses for different service platform.The service efficiency and success of rehabilitation treatment of different service platforms were compared.Results:53 patients satisfied for the service platform of rehabilitation information(94.64%)in the observation group,which was higher than that in the control group,and the difference was statistically significant(x2=13.187,P＜0.05).The scores of convenience,flexibility and continuity of patients of observation group were respectively(94.74±3.36),(94.57±4.12)and(93.65±4.02)for the platform,which were higher than those of control group,and the differences were statistically significant(t=14.279,13.604,13.553,P＜0.05).The acceptance scores of maintainers for platform,relevant operators,doctors and nurses were respectively(91.69±2.11),(94.52±3.65),(93.87±4.58)and(94.74±3.36)for the personalized service platform for rehabilitation information of the observation group,which were higher than those in the control group,and the differences were statistically significant(t=15.280,13.236,12.373,14.279,P＜0.05).The waiting time,appointment time and treatment cycle of patients in observation group were respectively(4.65±2.01)min,(3.21±1.03)d and(13.02±1.02)months,which were shorter than those of control group,and the differences were statistically significant(t=9.768,11.401,12.300,P＜0.05).Conclusion:The personalized service platform based on the IoTs technique of medical equipment for rehabilitation information can take doctors to timely adjust rehabilitation programs by combining the dynamic changes of patients'information,and formulate targeted rehabilitation guidance,and provide effective rehabilitation treatment for patients,and reduce waiting time of patient,and shorten the rehabilitation cycle.

Objective To investigate the influence of early growth response gene-1 (EGR1) on the autophagy of host cells following infection with human T cell leukemia virus type 1 (HTLV-1).MethodsA HTLV-1-positive cell line MT2 was co-cultured with HeLa cells for 24 h to construct the virus early infection model.Immunoblotting assay was used to detect the expression of HTLV-1 core protein p19 and EGR1.Luciferase reporter gene analysis was used to detect the transcriptional activity of 5′-regulatory sequence of EGR1 at different time points after co-culturing.An effective small interfering RNA (siRNA) targeting EGR1 was screened out and transfected into HeLa cells by Lipofectamine 2000.Then the transfected HeLa cells were co-cultured with the HTLV-1-positive cell line MT2 for 24 h.Immunoblotting assay was used to detect HTLV-1 core protein p19, EGR1 and autophagy-related protein LC3.Real-time PCR was performed to detect viral load.Autophagosome was analyzed by immunofluorescence after co-culturing.Results The expression of EGR1 and the transcriptional activity of pEGR1-luc gradually increased after co-culturing HeLa cells with MT2 cells for 8 h (P<0.01).The expression of EGR1 was positively correlated with host cell autophagy following HTLV-1 infection.The effective siRNA for silencing the expression of EGR1 was obtained and named as siE2.The viral load, the expression of HTLV-1 core protein p19 and the proportion of LC3B/LC3A in the co-culture model were markedly down-regulated by RNA interference with siE2, which was concomitant with a persistent decrease of intracellular autophagosome (P<0.01).Conclusion EGR1 is associated with host cell autophagy and viral replication in HTLV-1 infection.

Objective To study the role of endothelial cells on the inflammatory cytokine release in septic shock through the septic shock serum stimulating human primary endothelial cells (HPAEC) and peripheral blood mononuclear cells(PBMC).Methods PBMC isolated from healthy people by density gradient centrifugation.HPAEC cell surface markers CD144 and von Willebrand factor(vWF) molecule expression by RT-PCR and Western blot.Serum levels of IL-6,TNF-α,MCP-1 from septic shock patients and healthy human detected by ELISA.HPAEC and PBMC were stimulated with the isolated serums and LPS,respectively.ELISA was used to detect the supernatant IL-6,TNF-α,MCP-1 levels.HPAEC membrane molecules ICAM-1 expression was detected by flow cytometry with serum shock and LPS stimulation.Supernatant levels of IL-6,TNF-α,MCP-1 of HPAEC with S1P1 receptor agonist CYM-5442 pretreatment was detected by ELISA after shock serum stimulation.Results Endothelial cell markers CD144 and vWF molecules could be detected in the HPAEC.Levels of inflammatory cytokines IL-6,TNF-α,MCP-1 in patients with septic shock serum were significantly higher than healthy people (P＜0.01).PBMC and HPAEC with LPS or shock serum treatment respectively,compared with normal group,levels of inflammatory cytokines in the culture supernatant were significantly higher(P＜0.01).For PBMC,the level of inflammatory cytokines between shock group and LPS group were not significantly different (P＞0.05).But for HPAEC,levels of inflammatory cytokines in the supernatant of the shock group compared to the LPS group was significantly higher (P＜0.01).Similarly,when two cells after LPS stimulation,IL-6,TNF-α levels of HPAEC's supernatant were significantly lower than PBMC' s (P＜0.01),MCP-1 levels was no difference (P＞ 0.05).But when the stimulation of shock serum,HPAEC of IL-6,TNF-α levels and PBMC no significant difference (P ＞0.05).MCP-1 was significantly increased (P＜0.01).Shock patients serum stimulation S1P1 receptorspecific agonist CYM-5442 pretreatment of HPAEC with pretreatment of S1P1 receptor specific agonist CYM-5442,the culture supernatant of inflammatory cytokines IL-6,TNF-α,MCP-1 levels were significantly lower (P＜0.01).Conclusion Endothelial cells may play a central role on the release of inflammatory cytokine during septic shock.

Objective To research the relation of early growth response gene-1(EGR-1) and NF-κB in human T-cell leukemia virus type 1(HTLV-1) Tax protein positive cells. Methods RT-PCR was used to amplify the aimed segments EGR-1 cDNA which was then inserted into an eukaryotic expression plasmid pcDNA3.0 to construct pcDNA3.0-EGR-1. The constructed plasmid was transfected into TaxN and TaxP cells by Tfx-50-mediated transfer method, the expression levels of EGR-1, p65 and Tax mRNA in transfected cells were assay by RT-PCR after 48 h post-transfection, the proteins of EGR-1 and p65 were detected by Western blot after 48 h post-transfection too. The constructed plasmid and pNF-κB-luc reporter gene plasmid was co-transfected into TaxN and TaxP cells by Tfx-50-mediated transfer method, and the activity of luciferase was assay after 48 h post-transfection. Results The results showed that the eukaryotic expression plasmid pcDNA3.0-EGR-1 was successfully constructed. The mRNA and protein expression of EGR-1 could be promoted significantly by Tax. EGR-1 can promote the mRNA and protein expressions of p65 in TaxP cells, the activity of NF-κB was up-regulated by EGR-1 too. Conclusion EGR-1 maybe involve in adult T-cell leukemia(ATL) by increasing the activation of NF-κB.