1.Peyton's Four-Step Teaching Method for Intestinal Ultrasound Training: Efficacy and Practical Implications
Zihan NIU ; Xiaoyan ZHANG ; Zhaojue WANG ; Qingli ZHU ; Mengsu XIAO ; Li MA ; Yudi HE ; Wenbo LI
Medical Journal of Peking Union Medical College Hospital 2026;17(2):591-596
To evaluate the application value of the Peyton four-step teaching method in the standardized training of intestinal ultrasound and compare it with traditional teaching methods, so as to provide an optimized approach for clinical ultrasound training. Participants from the Department of Ultrasound at Peking Union Medical College Hospital between September 2024 and March 2025 were randomly assigned to either the traditional group or Peyton group. The traditional group followed the conventional "lecture- demonstration-practice" model, while the Peyton group implemented the standardized "demonstration-deconstruction-comprehension-execution" four-step approach. All training focused on standard intestinal ultrasound scanning techniques. After the training, the operational skills were independently evaluated by the instructors. To verify the reproducibility of the teaching method, the participants in traditional teaching group received additional Peyton method training after the initial assessment and underwent a second evaluation. A total of 18 participants were included in this study, with 9 in the traditional teaching group and 9 in the Peyton teaching group. Participants in the Peyton group demonstrated significantly higher scores than those in the traditional group at every anatomical site assessed (all The Peyton four-step method is significantly more effective than traditional teaching in improving residents' intestinal ultrasound skills, demonstrating its suitability as the preferred approach for standardized training programs.
2.Exploring the Effectiveness of A Mixed-Level Group Teaching Model in On-site Intestinal Ultrasound Training
Xiaoyan ZHANG ; Zhaojue WANG ; Zihan NIU ; Qingli ZHU ; Mengsu XIAO ; Li MA ; Yudi HE ; Wenbo LI
Medical Journal of Peking Union Medical College Hospital 2026;17(5):1470-1476
To investigate the effectiveness of a teaching model combining real-case bedside instruction with mixed-level group teaching in intestinal ultrasound training, and to provide reference forestablishing an efficient and practical training system for intestinal ultrasound. Ultrasound physicians with junior- and intermediate professional titles who attended training at the Department of Ultrasound, Peking Union Medical College Hospital in March 2025 were enrolled. A mixed-level group teaching model was implemented under real clinical scenarios in teaching clinics, with on-site practical instruction covering case analysis, clarification of examination purposes, real-time image interpretation, and standardized report writing. Teaching effectiveness was evaluated through pre- and post-training theoretical tests (covering basic knowledge, image recognition, and disease-related knowledge), self-assessment of confidence in performing examinations, and feedback on teaching satisfaction. A total of 12 trainees were enrolled, with a mean age of (35.2±3.8) years; 10 trainees (83.3%) were attending physicians and 2 (16.7%) were residents. The trainees' overall theoretical test scores improved significantly after training [(89.2±9.0) The teaching model combining real-case bedside instruction with mixed-level group teaching significantly enhances trainees' image interpretation skills and clinical competence in intestinal ultrasound, effectively addresses the diverse learning needs of trainees at different baseline levels, and provides a reference for developing effective clinical training approaches in intestinal ultrasound practice.
3.Relationship of the Cyclin B2 and Cyclin E1 expression with clinicopathological characteristics and 5-year survival rate of patients with hepatocellular carcinoma
Li FANG ; Yunxiao TIAN ; Qingli GAO ; Guangxu NIU
Journal of Clinical Surgery 2025;33(9):981-984
Objective To investigate the expressions of cyclin B2(CCNB2)and cyclin E1(Cyclin E1)in hepatocellular carcinoma(HCC)and their significance for the prognosis of HCC.Methods Sixty HCC patients were admitted and treated from October 2013 to October 2016.The expressions of CCNB2 and Cyclin E1 in 60 HCC tissues and adjacent normal tissues were detected by immunohistochemistry,and the relationship between the two and the clinicopathological characteristics of HCC patients was analyzed;Spearman method was performed to analyze the correlation of CCNB2 and Cyclin E1 expression;Kaplan-Meier was performed to analyze the relationship between CCNB2,Cyclin E1 expression and clinical prognosis;and univariate and multivariate Cox regression were performed to analyze the influencing factors of clinical prognosis of HCC patients.Results The positive expression rates of CCNB2 and Cyclin E1 in HCC(61.67％,56.67％)were significantly higher than those in adjacent normal tissues(20.00％,13.33％,P＜0.05);the positive expressions of CCNB2 and Cyclin E1 were positively correlated with serum AFP level,liver cirrhosis status,tumor thrombus and recurrence(P＜0.05).The expressions of CCNB2 and Cyclin E1 in HCC tissues were positively correlated(r=0.487,P=0.000).The positive expressions of CCNB2 and Cyclin E1 in HCC tissues predicted a lower survival time(x2=7.104,7.140,P=0.008,0.008).In addition,the positive expressions of CCNB2 and Cyclin E1 were independent factors affecting the prognosis of HCC patients(P＜0.05).Conclusion CCNB2 and Cyclin E1 are highly expressed in HCC and are associated with serum AFP level,cirrhosis status,tumor thrombus and recurrence,and can be used as independent prognostic markers.
4.Relationship of the Cyclin B2 and Cyclin E1 expression with clinicopathological characteristics and 5-year survival rate of patients with hepatocellular carcinoma
Li FANG ; Yunxiao TIAN ; Qingli GAO ; Guangxu NIU
Journal of Clinical Surgery 2025;33(9):981-984
Objective To investigate the expressions of cyclin B2(CCNB2)and cyclin E1(Cyclin E1)in hepatocellular carcinoma(HCC)and their significance for the prognosis of HCC.Methods Sixty HCC patients were admitted and treated from October 2013 to October 2016.The expressions of CCNB2 and Cyclin E1 in 60 HCC tissues and adjacent normal tissues were detected by immunohistochemistry,and the relationship between the two and the clinicopathological characteristics of HCC patients was analyzed;Spearman method was performed to analyze the correlation of CCNB2 and Cyclin E1 expression;Kaplan-Meier was performed to analyze the relationship between CCNB2,Cyclin E1 expression and clinical prognosis;and univariate and multivariate Cox regression were performed to analyze the influencing factors of clinical prognosis of HCC patients.Results The positive expression rates of CCNB2 and Cyclin E1 in HCC(61.67％,56.67％)were significantly higher than those in adjacent normal tissues(20.00％,13.33％,P＜0.05);the positive expressions of CCNB2 and Cyclin E1 were positively correlated with serum AFP level,liver cirrhosis status,tumor thrombus and recurrence(P＜0.05).The expressions of CCNB2 and Cyclin E1 in HCC tissues were positively correlated(r=0.487,P=0.000).The positive expressions of CCNB2 and Cyclin E1 in HCC tissues predicted a lower survival time(x2=7.104,7.140,P=0.008,0.008).In addition,the positive expressions of CCNB2 and Cyclin E1 were independent factors affecting the prognosis of HCC patients(P＜0.05).Conclusion CCNB2 and Cyclin E1 are highly expressed in HCC and are associated with serum AFP level,cirrhosis status,tumor thrombus and recurrence,and can be used as independent prognostic markers.
5.The E248R protein of African swine fever virus inhibits the cGAS-STING-mediated innate immunity.
Yinguang LIU ; Wenping YANG ; Yuan WEN ; Qingli NIU ; Jifei YANG ; Guiquan GUAN ; Hong YIN ; Haixue ZHENG ; Dan LI ; Zhijie LIU
Chinese Journal of Biotechnology 2022;38(5):1837-1846
We researched the mechanism of African swine fever virus (ASFV) protein E248R in regulating the cGAS-STING pathway. First, we verified via the dual-luciferase reporter assay system that E248R protein inhibited the secretion of IFN-β induced by cGAS-STING or HT-DNA in a dose-dependent manner. The relative quantitative PCR analysis indicated that the overexpression of E248R inhibited HT-DNA-induced transcription of IFN-b1, RANTES, IL-6, and TNF-α in PK-15 cells. Next, we found that E248R interacted with STING by co-immunoprecipitation assay and laser confocal microscopy. Finally, we demonstrated that E248R inhibited the expression of STING protein by using Western blotting. We demonstrated for the first time that the E248R protein of ASFV suppressed the host innate immune response via inhibiting STING expression. The results are pivotal in extending the understanding of the ASFV immune escape and can guide the design of vaccines against ASFV.
African Swine Fever Virus/genetics*
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Animals
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DNA
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Immunity, Innate
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Nucleotidyltransferases/metabolism*
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Signal Transduction
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Swine
6.Prokaryotic Expression of Bm86 Gene of Boophilus microplus and Optimization of the Expression Condition
Miling MA ; Guiquan GUAN ; Youquan LI ; Aihong LIU ; Qiaoyun REN ; Qingli NIU ; Hong YIN ; Jianxun LUO
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(06):-
A pair of specific primers was designed based on the reported Bm86 gene of Boophilus microplus,the Bm86 gene was cloned by PCR using the plasmid pMD18-T-Bm86 as templates,and subcloned into the prokaryotic plasmid pGEX-4T-1.The recombined plasmid was transformed into E.coli BL21(DE3) and followed by expression of the protein induced by different concentration of IPTG for different time.SDS-PAGE showed that the recombinant plasmid pGEX-4T-1/Bm86 expressed a fusion protein Bm86-GST(Mr 94 000) after being induced with IPTG.High level expre-ssion of Bm86-GST was found at 1 mmol/L IPTG condition after incubation for 8 h at 37 ℃,and the expression level of the recom-binant Bm86-GST reached up to 29% of total E.coli proteins.Western-blotting analysis showed that the recombinant Bm86-GST was recognized by the rabbit anti-B.microplus positive serum.
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