ObjectiveTo identify the primary hemostatic active components in Moutan Cortex Carbonisata（MCC） based on the spectrum-effect relationship between the fingerprint and hemostatic efficacy， thereby providing a basis for characterizing its active constituents. MethodsUltra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry（UPLC-Q-Orbitrap MS/MS） was employed to establish the fingerprint profiles of 16 batches of MCC aqueous extracts and identify the common peaks. Activated partial thromboplastin time（APTT）， an in vitro coagulation activity indicator， was measured for the 16 batches of samples using a semi-automated coagulometer. Grey relational analysis（GRA）， Pearson correlation analysis， and partial least squares regression（PLSR） were comprehensively applied to screen potential hemostatic active components. For the identified active components， multi-dimensional pharmacological validation was conducted through in vitro coagulation assays measuring APTT， prothrombin time（PT）， and thrombin time（TT）， evaluation of hemostasis rate using a zebrafish cerebral hemorrhage model， and real-time quantitative polymerase chain reaction（Real-time PCR） detection of coagulation factor X（FⅩ） mRNA expression level. ResultsThe UPLC fingerprint of the aqueous extract of MCC was successfully established， identifying 12 common peaks. Among these， 9 chemical components were subsequently characterized using UPLC-Q-Orbitrap MS. Comprehensive application of GRA， Pearson correlation analysis， and PLSR analysis identified 5-hydroxymethylfurfural（5-HMF）， gallic acid， 1-O-galloylglucose， and p-hydroxybenzoic acid as key hemostatic active constituents in MCC. In vitro coagulation assays confirmed that all four active components significantly shortened APTT and PT（P<0.05， P<0.01）. The zebrafish cerebral hemorrhage model further validated their in vivo hemostatic efficacy， with each component significantly reducing hemorrhage area（P<0.05， P<0.01）， yielding hemostasis rates of 31.20% for 5-HMF， 68.85% for gallic acid， 45.45% for 1-O-galloylglucose， and 45.60% for p-hydroxybenzoic acid， and demonstrating overall concentration-dependent effects. Real-time PCR analysis demonstrated that all active components significantly upregulated FⅩ mRNA expression（P<0.05， P<0.01）， synergistically enhancing hemostasis. ConclusionBy integrating spectrum-effect relationship analysis and multi-dimensional efficacy validation， this study identified four hemostatic constituents from MCC， providing a scientific basis for elucidating its hemostatic material basis.

OBJECTIVE:To optimize the drying technology of Zingiber officinale peel and establish its quality standard. METHODS:Moisture content was determined in samples after being dried for different time(0.5-10.0 h)under 50,60,70,80, 90 ℃. Optimal drying time under each temperature was screened by using moisture content of 7%-13% as dryness for controlling standard. Then contents of 6-gingerol,8-gingerol,6-shogaol,10-gingerol in samples dried for optimal drying time under different temperatures were measured,using the 4 gingerol contents as indexes to optimize the drying temperature and time. And verification test was conducted. The moisture,total ash,water soluble extract,volatile oil,6-gingerol,8-gingerol and 10-gingerol of Z. offici-nale peel from 10 different producing areas were detected to establish quality standard after being dried with the optimal technology. RESULTS:The drying time of Z. officinale peel under 50,60,70,80,90 ℃ was determined as 10.0,4.2,2.6,1.5,1.1 h,re-spectively. The optimal drying technology was 50 ℃ drying for 10.0 h. Verification test showed RSDs of 6-gingerol,8-gingerol, 6-shogaol,10-gingerol contents were 1.46%,1.09%,1.35%,1.12%(n=3),respectively. The quality standard of Z. officinale peel was suggested that total ash was no more than 18.0%;water soluble extract,volatile oil,6-gingerol,8-gingerol,10-gingerol were respectively no less than 18.0%,1.30%,0.730%,0.060%,0.100%. CONCLUSIONS:The optimized drying technology of Z. officinale peel is reasonable,reliable,stable and simple,which provides a scientific basis for standardizing the drying technolo-gy and quality standard of Z. officinale peel. The established quality standard is feasible.