Objective To investigate the effects of gypenoside(Gyp)on proliferation,migration and invasion of hu-man prostate cancer cell line PC-3.Methods PC-3 cells were treated with 200-1 200 μg/mL Gyp to determine the optimal concentration.PC-3 cells were divided into control,Gyp-L group(400 μg/mL),Gyp-M group(600 μg/mL)and Gyp-H group(800 μg/mL),and Gyp-H+8-bromo-cAMP group(100 μmol/L PKA activator).Colony formation assay was applied to detect cell proliferation.Apoptosis was measured by flow cytometry.Scratch experiment was applied to detect the migration ability.Transwell assay was applied to detect the invasive ability.ELISA experiment was applied to detect cAMP level in each group.Western blot was applied to detect the expres-sion of E-cadherin,N-cadherin,Snail,PKA and CREB proteins.Results Gyp inhibited PC-3 proliferation in a concentration dependent manner.Gyp concentration of 400,600 and 800 μg/mL was selected for subsequent experiments.Compared with the control group,colony formation,number of invasive cells,scratch healing rate,the levels of Snail,N-cadherin,PKA,cAMP,and CREB proteins in the Gyp-L,Gyp-M,and Gyp-H groups were significantly reduced(P＜0.05),but the apoptosis rate as well as the level of E-cadherin protein were significantly increased(P＜0.05).PKA activator attenuated the inhibitory effect of Gyp on the malignant behavior of prostate cancer cells.Conclusions Gyp inhibits proliferation,migration and invasion,and promotes apoptosis in prostate cancer cell line PC-3.

Objective:To investigate the relationship between oxygen consumption (VO 2), carbon dioxide production (VCO 2), and Oxygen Consumption/lactate (VO 2/Lac) with risk of death in patients with severe ARDS. Methods:A retrospective cohort study method was used, and the study subjects were hospitalized for >5 days adult patients with severe ARDS in the central intensive care unit of Henan Provincial People's Hospital from 1 March 2020 to 30 June 2023. The following patients were excluded: IC test was not completed on the 4th day of ICU admission, IC test results were unreliable, mechanical ventilation duration had exceeded 48 h at the time of ICU transfer or admission, palliative care patients and pregnant and parturient women. Using indirect calorimetry to determine VO 2 and VCO 2 values on the 4th day of admission, reviewing medical records to obtain general condition, disease information, blood gas analysis (including lactate value), diagnostic and therapeutic measures, and following up deaths by telephone and time of death. The primary outcome measure was death at 90 days, and the secondary outcome measure was death at 28 days, length of stay in ICU, total length of stay, and total hospitalization cost. Cox regression analysis and linear regression analysis were used to investigate the relationship between VO 2, VCO 2, VO 2/Lac and primary and secondary outcome indexes. Results:A total of 216 patients were enrolled, 78 patients (36.1%) died and 138 patients (63.9%) survived at 90 days. After correction for confounders, the results of multifactorial Cox regression analysis suggested that compared with the Q4 group, HR (95% CI) for 90-day risk of death in the VO 2 Q1 and Q2 groups was 3.21 (1.38, 7.49) and 3.24 (1.42, 7.38), and HR (95% CI) for 90-day risk of death in the VCO 2 Q1, Q2 and Q3 groups was 5.88 (2.33, 14.84), 4.26 (1. 60, 11.34) and 3.54 (1.34, 9.35), respectively, and the HR (95% CI) for 90-day risk of death in the VO 2/Lac Q1, Q2 and Q3 groups were 8.72 (3.01, 25.25), 8.43 (2.91, 24.47) and 4.04 (1.34, 12.17) respectively. P-trends were all <0.05, indicating that VO 2, VCO 2 and VO 2/Lac were linearly and negatively associated with the risk of 90-day mortality. In addition, VO 2, VCO 2, and VO 2/Lac were negatively associated with 28-day risk of death and higher VO 2/Lac was negatively associated with length of ICU stay. Conclusions:VO 2, VCO 2 and VO 2/Lac were negatively associated with 90-day mortality risk and 28-day mortality risk in patients with severe ARDS and may be independent risk factors predicting mortality risk of such patients.

Objective:To investigate the correlation of the blood concentration of tacrolimus ( Tac) and serum cystatin C ( SCysC) and serum creatinine (Scr) in the patients with renal transplantation. Methods:Totally 84 cases of renal transplantation patients (67 male/17 female) treated with Tac were involved. The blood concentration of Tac, SCysC and Scr were monitored after the operation. Data results were categorized according to the postoperative time and the blood concentration. The correlations of Tac blood concentra-tion,SCysC and Scr were analyzed and compared by using Pearson correlation analysis. Results: The blood concentration of Tac was not significantly associated with SCysC and Scr in different postoperative time groups and different drug concentration groups ( P >0. 05). As the extension of time,the blood concentration of Tac showed a gradual declining trend, while SCysC and Scr levels de-creased first, and then increased gradually after two years of the operation. Conclusion:The blood concentration of Tac has no effect on the function evaluation of transplanted kidney by the biochemical indicators such as SCysC and Scr.