Objective:To explore the expression of Aurora Kinase A (AURKA) in hepatocellular carcinoma (HCC) and its prognostic value.Methods:mRNA expression profiles and clinical data of HCC patients were downloaded from the Cancer Genome Atlas (TCGA) database. Expression of AURKA mRNA in HCC patients of TCGA database from normal liver tissue and all tumor tissues, normal tissues adjacent to cancer and matched tumor tissues were analyzed, and then expression of AURKA to was investigated in HCC tissues and normal liver tissues in the Human Protein Atlas (HPA) database. According to the TNM stage information of HCC patients in TCGA database, expression of AURKA in different stages was analyzed. Kaplan-Meier method was used to analyze whether the high and low expression of AURKA in HCC patients of TCGA database (with the median as the cut-off value) was significantly related to the length of survival. The RNA-seq expression profile data of HCC patients in the public resource platform of the Kaplan-Meier Plotter website was used for external verification. Cox univariate and multivariate analysis were performed on the age, sex, degree of differentiation, TNM stage, and AURKA mRNA expression of TCGA database patients.Results:374 cases of HCC tumor tissues and 50 cases of adjacent normal liver tissues in the TCGA database were included. All HCC tumor tissues in the TCGA database compared with the paired adjacent tissues mRNA level of AURKA was significantly increased, and the protein level was also increased, the difference was statistically significant ( P<0.05); With the tumor TNM stage increase of AURKA mRNA expression showed a gradual upward trend, and the difference was statistically significant ( P<0.05); in the TCGA database HCC cohort, high expression of AURKA mRNA was associated with poor HCC prognosis, and was obtained in Kaplan Meier Plotter database. The difference was statistically significant ( P<0.05); Cox multivariate regression analysis showed that TNM stage ( HR=1.69, 95% CI: 1.37-2.10) and AURKA mRNA expression level ( HR=1.03, 95% CI: 1.01-1.10) are the independent prognostic factors of HCC patients. Conclusions:AURKA is highly expressed in HCC, which is associated with the poor prognosis of HCC. AURKA is an independent prognostic factor of HCC.

Objective To discuss the effect and value of wrapping ureter by a pedicled gastrocolic omentum flap during cutaneous ureterostomy as urinary diversion after radical cystectomy.Methods The clinical data of 12 cases(7 males and 5 females)with bladder cancer during January 2016 and September 2017 were retrospectively analyzed.The median age was 77 years (range 75-83),and the course of the disease was 5 days to 2 years.11 cases had gross hematuria,5 cases had frequent and urgent urination,1 case had lower abdominal pain,and 1 case had no obvious clinical symptoms.9 cases had a history of transurethral prostatic resection.8 cases had a complication of hypertension,5 cases had a complication of diabetes,7 cases had a complication of cardiovascular and cerebrovascular diseases,and 6 cases had a complication of anemia.The ASA level was 3 in 9 cases and was level 4 in 3 cases.All the patiants were diagnosed of bladder urothelial carcinoma by biopsy.There were 8 cases with T3 tumor and 4 cases with T4.All the patients underwent laparoscopic radical cystectomy under general anesthesia,and wrapping ureter by a pedicled gastrocolic omentum flap during cutaneous ureterostomy was used for urinary diversion.Take a segment of 3-5 cm wide pedicle omentum,which is covered from the distal end of the ureter to the end,and use a 4-0 absorbable line to fix the omentum and ureteral outer membrane,and then perform ureterocutaneostomy.The incidence of postoperative anastomotic stenosis,hydronephrosis and wound infection were observed.Results All the operations were performed successfully without obvious operative complications.The mean operating duration was125 (95-217) mins,estimated volume of blood loss 120 (50-600)ml.5 days after surgery,1 patient developed incision infection,which was cured by strengthening anti-infection drug therapy and wound dressing.No complications such as urinary fistula,intestinal fistula and peripheral hernia occurred.No death was reported during perioperative period.Single J tube was removed 1-3 months after surgery.Within a follow-up of 12 (3-23) months,1 case died of cerebral infarction 3 months after surgery,1 case occurred papilla light constriction without special treatment,no ureteral stenosis and necrosis cases and there were no cases of hydronephrosis.All the patients were satisfied with the quality of life after surgery.Conclusions Wrapping ureter by a pedicled gastrocolic omentum flap during cutaneous ureterostomy is simple,safe and effective as urinary diversion after radical cystectomy.

Objective To study the effect of hyperbaric oxygen ( HBO) on the atherosclerosis ( AS) and the possible mechanism in apolipoprotein E ( apoE )-knockout mice by observing the variation of atherosclerotic plaque pathological morphology , blood lipid and related inflammatory cytokines .Methods Twenty-eight 8-week old apoE-knockout male mice were fed with western-diet for 8 weeks.Four mice were randomly sacrificed to collect aortas for Hematoxylin and Eosin ( HE ) staining , and the AS model was ascertained under microscopy .Then, the remaining 24 mice were randomly assigned into the ( HBO) group (n=12) and the normal control group (n=12).The animals in the HBO group were treated with HBO once a day for 30 days and the animals in the control group were left there without HBO treatment .The body mass of the mice were weighed both before and after termination of HBO treatment .The day after termination of HBO treatment, the mice were sacrificed for the detection of blood lipids and serum C-reactive protein (CRP). Histopathological features of aorta were defined by Hematoxylin and Eosin (HE) staining.Subsequently, the area ratio of plaque and lumen and the ratio of intimal and media areas were analyzed by the image processing software.Results Following HBO treatment, there were no significant differences in blood lipid levels , when comparisons were made between the HBO group and the control group (P>0.05), however, the level of serum CRP in the HBO group was significantly lower than that of the control group (P<0.05).The HE staining indicated that large quantities of atherosclerotic plaques in the aorta of the control group and intima were thickened , and the area ratio of plaque and lumen and the ratio of intimal area and media area was obviously larger than those of the animals in the HBO group ( P <0.05).Conclusions HBO could inhibit the development of atherosclerosis , which was not related to the modulation of blood lipid .The effect of HBO on anti-inflammation might be related to further progress of atherosclerosis .

Objective To study the effect of hyperbaric oxygen ( HBO) on the atherosclerosis ( AS) and the possible mechanism in apolipoprotein E ( apoE )-knockout mice by observing the variation of atherosclerotic plaque pathological morphology , blood lipid and related inflammatory cytokines .Methods Twenty-eight 8-week old apoE-knockout male mice were fed with western-diet for 8 weeks.Four mice were randomly sacrificed to collect aortas for Hematoxylin and Eosin ( HE ) staining , and the AS model was ascertained under microscopy .Then, the remaining 24 mice were randomly assigned into the ( HBO) group (n=12) and the normal control group (n=12).The animals in the HBO group were treated with HBO once a day for 30 days and the animals in the control group were left there without HBO treatment .The body mass of the mice were weighed both before and after termination of HBO treatment .The day after termination of HBO treatment, the mice were sacrificed for the detection of blood lipids and serum C-reactive protein (CRP). Histopathological features of aorta were defined by Hematoxylin and Eosin (HE) staining.Subsequently, the area ratio of plaque and lumen and the ratio of intimal and media areas were analyzed by the image processing software.Results Following HBO treatment, there were no significant differences in blood lipid levels , when comparisons were made between the HBO group and the control group (P>0.05), however, the level of serum CRP in the HBO group was significantly lower than that of the control group (P<0.05).The HE staining indicated that large quantities of atherosclerotic plaques in the aorta of the control group and intima were thickened , and the area ratio of plaque and lumen and the ratio of intimal area and media area was obviously larger than those of the animals in the HBO group ( P <0.05).Conclusions HBO could inhibit the development of atherosclerosis , which was not related to the modulation of blood lipid .The effect of HBO on anti-inflammation might be related to further progress of atherosclerosis .

Objective To summarize experience of diagnosis and treatment of the mucin-producing bile duct tumors(MPBTs). Methods Clinicopathological features of 7 patients with MPBT undergoing surgery from Nov 2002 to May 2005,were retrospectively reviewed.The clinical radiography characteristics and the resection type were summarized respectively. Results Fluctuant iaundice was the most common manifestation of MPBTs,with different characteristics of magnetic resonance cholangiopancreatography when compared with gallbladder carcinoma,hilar cholangiocarcinoma and distal bile duct cancer.All the 7 patients with MPBT underwent successful surgical resection and were cured. Conclusion Appropriate diagnosis and treatment of MPBTs made it possible to achieve long-term survival of these patients.