AIM To evaluate the quality of Gegen Formula Granules.METHODS Linear calibration with two reference substances(LCTRS)was adopted in the predicting of retention time with puerarin and daidzein as internal standards.UPLC characteristic chromatograms were established.The contents of 3'-hydroxy puerarin,puerarin(internal standard),3'-methoxy puerarin,puerarin 6"-O-xyloside,puerarin apioside and daidzin were determined by quantitative determination analysis multi-components by a single marker(QAMS),after which their transfer rates were calculated.RESULTS Compared with relative retention time method,LCTRS demonstrated higher positional accuracy for characteristic peaks and wider application range for columns.There were 9 characteristic peaks in the characteristic chromatograms for 14 batches of formula granules and 15 batches of standard decoctions with the similarities of more than 0.95.The contents and transfer rates of various constituents in formula granules and standard decoctions were basically consistent.CONCLUSION The chemical constituents in formula granules and their standard decoctions of Puerariae lobatae Radix display good consistency,reliable preparation process is observable in the former.

With the rapid development of generative artificial intelligence(GAI)technologies,their widespread application in academic research and writing is continuously expanding the boundaries of sci-entific inquiry.However,this trend has also raised a series of ethical and regulatory challenges,inclu-ding issues related to authorship,content authenticity,citation accuracy,and accountability.In light of the growing involvement of AI in generating academic content,establishing an open,controllable,and trustworthy ethical governance framework has become a key task for safeguarding research integrity and maintaining trust within the academic community.This expert consensus outlines ethical requirements across key stages of AI-assisted academic writing-including topic selection,data management,citation practices,and authorship attribution.It aims to clarify the boundaries and ethical obligations surrounding AI use in academic writing,ensuring that technological tools enhance efficiency without compromising in-tegrity.The goal is to provide guidance and institutional support for building a responsible and sustainable research ecosystem.

Adenosine triphosphate(ATP),as the core energy metabolism molecule in living systems,has dynamic changes closely related to fundamental physiological processes.To meet the urgent demand for spatiotemporal ATP detection in vivo and in situ,the development of highly sensitive multifunctional synchronous sensing fluorescent probes has become a recent research focus.These dual-function probes achieve fluorescence detection of dual targets by designing recognition sites for ATP alongside biological factors or microenvironment parameters such as reactive oxygen/nitrogen/sulfur species,metal ions,and enzymes,enabling physiological/pathological state correlation analysis through bioimaging.This paper systematically reviews recent advances in fluorescent probes for the collaborative detection of ATP and key biomolecules.It specifically examines probe construction strategies based on specific molecular recognition mechanisms(e.g.,metal coordination competition,electrostatic interactions,and host-guest recognition),multi-modal optical signal transduction mechanisms(ratiometric fluorescence,fluorescence lifetime,and photodynamic therapy),and their applications in pathological models such as oxidative stress monitoring,metal homeostasis imbalance,and enzyme activity co-detection.Finally,from the perspective of molecular probe engineering,current challenges and future research directions are proposed to provide methodological support for precise analysis of ATP-related life process regulation networks.

Objective:To compare the clinical efficacy and safety of minocycline in the treatment of macrolide-resistant Mycoplasma pneumoniae pneumonia (MRMP) in children of different age groups. Methods:The clinical data of MRMP cases treated with minocycline hydrochloride capsules in the Department of Infectious Diseases of Shanghai Children′s Medical Center Affiliated to Shanghai Jiao Tong University School of Medicine from June 2023 to June 2024 were collected through the hospital′s electronic medical record system. This study used a retrospective cohort study. According to the age of onset, they were divided into two groups: children <8 year-old-group and those ≥8 year-old-group. The indicators including the duration of temperature after medication, the recovery time of serum amyloid A (SAA) and C-reactive protein (CRP) were compared between the two groups. The adverse reactions during hospitalization and follow-up of six months after discharge were analyed. Non-parametric rank sum test was used for statistical comparison.Results:A total of 274 MRMP cases were included, including 151 in the <8-year-old group and 123 in the ≥8-year-old group. After minocycline hydrochloride capsule treatment, the duration of fever in the ≥8-year-old group was 1.5(1.0, 2.0) d, which was shorter than 2.0(1.0, 2.0) d in the <8-year-old group. The difference was statistically significant ( Z=-2.02, P=0.044). The recovery time for SAA (3.0(2.0, 6.0) d vs 5.0(3.0, 6.0) d) and CRP (3.0(2.0, 5.0) d vs 4.0(3.0, 6.0) d) in the <8-year-old group were both shorter than those in the ≥ 8-year-old group, and the differences were both statistically significant ( Z=-2.62, P=0.009 and Z=-3.10, P=0.002, respectively). During hospitalization, there were two cases of nausea, four cases of vomiting, one case of abdominal pain, with no rash, dizziness and tetracycline teeth in the <8-year-old group, and the incidence of adverse reactions was 4.6%(7/151). In the ≥8-year-old group, there were one case of nausea, one case of vomiting, one case of rash, one case of dizziness, with no abdominal pain and tetracycline teeth, and the incidence of adverse reactions was 3.3%(4/123). There were 253 cases of follow-up, of which 148 cases (58.5%) did not re-examine because of the normal blood routine at the last time during hospitalization, 105 cases (41.5%) re-examined blood routine, and the results were all normal. Twenty-five cases were normal in liver and kidney functions. Some children under six years old who did not change permanent teeth failed to clarify the impact of minocycline on teeth due to the short follow-up time. The total effective rate of minocycline in the two groups was consistent, both was 100.00%. Conclusions:Minocycline has a good clinical efficacy for MRMP. The total effective rate of clinical treatment for MRMP in children <8 years old and ≥8 years is consistent, with fewer adverse reactions and good safety. The impact on the teeth of children under six years old who have not replaced their deciduous teeth with permanent teeth requires further observation.

Objective To investigate the expression of serum long non-coding RNA X-inactive specific transcript(lncRNA XIST)and microRNA-126-3p(miR-126-3p)in patients with non-small cell lung cancer(NSCLC),and their relationship with recurrence after radical surgery.Methods A total of 108 NSCLC patients who underwent radical surgery and were admitted to General Hospital of Southern Theater Command of the People's Liberation Army of China from February 2019 to October 2020 were selected as the NSCLC group,and 52 healthy volunteers who underwent physical examination in this hospital were selected as the control group.qRT-PCR method was used to detect the relative expression levels of serum lncRNA XIST and miR-126-3p.Pearson method was used to analyze the correlation between serum lncRNA XIST and miR-126-3p expression.Logistic regression analysis was used to analyze the influencing factors of postoperative recurrence in NSCLC patients.Receiver operating characteristic(ROC)curve was used to evaluate the predictive value of serum lncRNA XIST and miR-126-3p on postoperative recurrence in patients.Results The expression level of serum lncRNA XIST in the NSCLC group was higher than that in the control group(P<0.05),while the expression level of miR-126-3p was lower than that in the control group(P<0.05).The expression levels of serum lncRNA XIST and miR-126-3p were related to TNM staging and lymph node metastasis(P<0.05).Bioinformatics website predicted that there was a targeted binding site between lncRNA XIST and miR-126-3p,and serum lncRNA XIST was negatively correlated with miR-126-3p expression(r=-0.579,P<0.05).Compared with the non-recurrent group,the expression level of serum lncRNA XIST in the recurrent group increased(P<0.05),while the expression level of miR-126-3p in the recurrent group decreased(P<0.05).Logistic regression analysis results showed that lncRNA XIST,miR-126-3p,lymph node metastasis,and TNM staging were the influencing factors for postoperative recurrence in NSCLC patients(P<0.05).The area under the curve(AUC)of serum lncRNA XIST,miR-126-3p,and the combination of the two in prediction of postoperative recurrence in NSCLC patients were 0.750,0.886,and 0.933,respectively,the combined prediction of the two was superior to individual prediction(Zcombination vs.lncRNA XIST=4.076,Zcombination vs.miR-126-3p=2.065,P<0.05).Conclusion The expression of serum lncRNA XIST is increased and miR-126-3p is decreased in NSCLC patients,both of which have certain predictive value for recurrence after radical surgery in patients.

Objective To develop a novel protective ventilator circuit component and to verify its performance by water seal and anti-splash experiments.Methods A novel protective ventilator circuit component had a design scheme with the multifunctional joint,and consisted of a tee connection tube,an isolation sleeve and a stop sleeve,of which,the tee connection tube was made of polyethylene polymer material and the others were made of silicone material.The tee connection tube had a T-shaped structure with two standard connection ports,which was composed of an adapter,a sealing cap,a plug and a sealing ring;the isolation sleeve was in the shape of a cylinder with a raised bottom,which was inserted into the adapter;the stop sleeve was located in the isolation sleeve,with an inverted frustum of a cone at the bottom and a rounded hole in the middle of the inverted frustum.An open ventilator circuit tube was involved in the performance verification of the circuit component developed.In the water seal experiment,sputum aspiration was simulated and the heights of the liquid level drop in the L-shaped tubes were compared after sputum aspiration.In the anti-splash experiment,the infection rates on the surfaces of the sterile hole towels and gloves were calculated.Results Water seal experiment showed after sputum aspiration the open ventilator circuit tube had the liquid level at the L-shaped tube higher significantly than that of the circuit component;the anti-splash experiment indicated sputum aspiration resulted in the occurance of the splashing out of the secretion and 77.5%infection rate by the open ventilator circuit tube,while no splashing out and 0%infection rate by the circuit component developed.Conclusion The novel protective ventilator circuit component behaves well in sealing and anti-splashing,and thus is worthy of clinical application for sputum aspiration.[Chinese Medical Equipment Journal,2025,46(4):113-117]

The working principle of Bausch&Lomb BL11110 phacoemulsification system was described.Three cases of typical faults of the phacoemulsification system were introduced,and the causes were analyzed,then the maintenance measures were given accordingly.References were provided for diagnosing and eliminating the faults of the phacoemulsification system.[Chinese Medical Equipment Journal,2025,46(4):118-120]

With the rapid development of generative artificial intelligence(GAI)technologies,their widespread application in academic research and writing is continuously expanding the boundaries of sci-entific inquiry.However,this trend has also raised a series of ethical and regulatory challenges,inclu-ding issues related to authorship,content authenticity,citation accuracy,and accountability.In light of the growing involvement of AI in generating academic content,establishing an open,controllable,and trustworthy ethical governance framework has become a key task for safeguarding research integrity and maintaining trust within the academic community.This expert consensus outlines ethical requirements across key stages of AI-assisted academic writing-including topic selection,data management,citation practices,and authorship attribution.It aims to clarify the boundaries and ethical obligations surrounding AI use in academic writing,ensuring that technological tools enhance efficiency without compromising in-tegrity.The goal is to provide guidance and institutional support for building a responsible and sustainable research ecosystem.