In this study, three new germacranolide sesquiterpenes (1-3), together with six related known analogues (4-9) were isolated from the whole plant of Carpesium cernuum. Their structures were established by a combination of extensive NMR spectroscopic analysis, HR-ESIMS data, and ECD calculations. The anti-leukemia activities of all compounds towards three cell lines (HEL, KG-1a, and K562) were evaluated in vitro. Compounds 1-3 exhibited moderate cytotoxicity with IC
Antineoplastic Agents, Phytogenic/pharmacology* ; Asteraceae/chemistry* ; Drug Screening Assays, Antitumor ; Humans ; K562 Cells ; Phytochemicals/pharmacology* ; Sesquiterpenes, Germacrane/pharmacology*

Objective To screen the risk factors of children septic shock and establish the children septic shock predictive model,so as to provide an early warning for the occurrence of disease.Methods One thousand five hundred and fifty eight cases of sepsis children,aged less than 14 and admitted to the 7 affiliated medical institutions of Chongqing Medical University from January 1,2015 to August 31,2019,were selected in present study.According to whether septic shock occurred during hospitalization,they were divided into septic shock group(study group,287 cases)and simple sepsis group(control group,1271 cases).The independent risk factors were selected using univariate analysis+logistic regression analysis,grid search algorithm was used to search for the optimal parameters of XGBoost algorithm,the prediction model was constructed by XGBoost algorithm.Results A total of 80 indicators were collected,14 indicators with a missing rate of>30%were excluded,and 66 indicators were finally included.Forty one differential indicators with statistical significance were screened by using univariate analysis,and 10 independent risk factors were screened by using logistic regression analysis,including:increased urine microalbumin,more common leukocytes,urine protein positive,low calcium ion,high lactate dehydrogenase,high uric acid,low albumin,high myoglobin,high creatine kinase isoenzyme MB,and high procalcitonin.The grid search showed the best model performs when the XGBoost algorithm parameter max_depth=6 and eta=0.1.The tested prediction model had an area under the curve(AUC)of 0.757,a sensitivity of 0.727,and a specificity of 0.768.The performance of the model was improved compared to that of previous studies.Conclusion The clinical prediction model constructed by"grid search+XGBoost"algorithm has a good prediction effect on septic shock in children,and can be used to predict children septic shock in Chongqing.

OBJECTIVE: To screen the differentially expressed proteins at the early stage of K562 cells treated with meisoindigo by using tandem mass tags (TMT)-based proteomics technology, and to explore the mechanism for meisoindigo-inducing apoptosis. METHODS: The half inhibitory concentration (IC) of mesoindigo on K562 cells was determined by CCK8. The flow cytometry was used to assay the apoptosis of K562 cells treated by meisoindigo or DMSO. Total proteins were extracted from the cells treated with 0.2% DMSO (control) or 20 μmol/L meisoindigo (Test) for 2 hours. Then, the TMT-labeling HPLC-MS/MS was used to identify and quantify the peptides and their abundance, all the tests were repeated for 3 times. The Mascot software was used to identify the proteins; the GO annotations, enrichment and cluster analysis were used to analyze the differentially expressed proteins. RESULTS: Meisoindigo-induced K562 cell apoptosis in a dose-dependent manner (r=0.98), 5 544 proteins were identified, 4792 of which were quantified. The protein with expression difference>1.5-folds in Test group accoanted for 8, out of which the expression of 4 proteins were up-regulated and 4 were down-regulated. The differentially expressed proteins mainly associated with reactive oxygen species (ROS). CONCLUSION Several proteins including DDIT4 were found to have dramatic changes in the early stage of K562 cells treated with meisoindigo by using quantitative proteomics technology. The ROS metabolic process may play important roles in meisoindigo-inducing apoptosis of K562 cells.
Apoptosis ; Humans ; Indoles ; K562 Cells ; Proteomics ; Tandem Mass Spectrometry

To assess the changes of sarcolemma Na+/K+ ATPase (CMNKA) and sarcoplasmic reticulum membrane Ca2+ ATPase (SERCA) activities after stem cells transplantation in heart failure. Rabbit was used as heart failure model by intravenously injecting adriamycin. Autologous bone marrow mononuclear cells (BMCs), bone marrow mesenchymal stem cells (MSCs) or skeletal myoblasts (SMs) were introduced into coronary arteies through the root of aorta when two balloons occluding just above sinus of Valsalva. After 4 weeks, left ventricular ejection fraction (LVEF)was evaluated by echocardiography, and the activities of CMNKA and SERCA were measured by colorimeter. In BMCs (n=8)and MSCs (n=8) group, LVEF were significantly improved (P < 0.05). No significant improvement were seen in SMs group (n=6) compared to sham group (n=8). The CMNKA activity in all stem cells groups was significantly increased compared to sham group (P < 0.05). Meanwhile, in comparison with sham group, the incremental tendencies of SERCA activity were seen in stem cells groups. In conclusion, stem cells transplantation could increase the activities of CMNKA and SERCA in heart failure, a possible mechanism to improve heart function.
Animals ; Doxorubicin ; Female ; Heart Failure ; chemically induced ; enzymology ; therapy ; Male ; Myocardium ; enzymology ; Rabbits ; Random Allocation ; Sarcolemma ; enzymology ; Sarcoplasmic Reticulum Calcium-Transporting ATPases ; metabolism ; Sodium-Potassium-Exchanging ATPase ; metabolism ; Stem Cell Transplantation

To investigate the feasibility of introcoronary cell infusion into nonischemic heart failure (HF) heart and whether different types of stem cell transplantation would affect heart function to a similar degree. Japanese white ears rabbits were used as HF models by intravenous injection adriamycin. Autologous bone marrow mononuclear cells(BMCs), bone marrow stromal cells (MSCs), skeletal myoblasts (SMs) or culture medium were infused into coronary arteries respectively by occluding the root of ascending aorta. The mortality during and 4 weeks after the procedure the mortality was 7.1% and 16.7% respectively. After 4 weeks, the ejection fraction (EF) in BMCs group had significant improvement (P < 0.05, n=8). No significant difference was seen in MSCs (n =8), SMs (n=6) and sham groups (n=8) compared with pretransplantation (P > 0.05). In sham group,the left ventricular endostolic diameter (LVED) had significant enlargement (P < 0.05), No significant difference was seen in MBCs, MSCs and SMs groups compared with pretransplantation (P > 0.05). Immunofluorescence revealed de novo expression of cardiac troponin I in BMCs and MSCs groups, cardiac troponin I was not detected in SMs group. In conclusions, intracoronary cell transplantation could provide effective cell delivery into dilated cardiomyopathy hearts and could be a useful strategy for treating CHF, BMCs cell transplantation may be the first choice in all the above cell types.
Animals ; Bone Marrow Transplantation ; Chronic Disease ; Coronary Vessels ; Heart Failure ; physiopathology ; surgery ; Infusions, Intra-Arterial ; Mesenchymal Stem Cell Transplantation ; Myoblasts, Skeletal ; transplantation ; Rabbits ; Random Allocation ; Stem Cell Transplantation ; methods ; Troponin ; metabolism ; Ventricular Function, Left ; physiology

Stem cells transplantation is a promising strategy for treating myocardial infarction and/or chronic heart failure; however, with respect to nonischemic heart failure, there are some limitations inherent in the current methods of transplantation. In this study, we investigated the feasibility of a novel method, i. e. transplantation through the root of aorta when the ascending aorta occluded above the sinus aortae. Japanese white ears rabbits were used as chronic heart failure models by intravenous injection of adriamycin. Autologous bone marrow mononuclear cells (MNC) were infused into the root of aorta when the ascending aorta was occluded by a couple of balloons above the sinus aortae. After 4 weeks, ejection fraction was significantly improved in MNC group. In conclusion, we have developed a unique method for efficient and safe cell transplantation based on infusion in aorta. This method, potentially suitable for nonischemic heart failure and could be used to achieve even and global supply of cells in heart.
Animals ; Aorta ; surgery ; Doxorubicin ; Feasibility Studies ; Heart Failure ; chemically induced ; surgery ; Rabbits ; Stem Cell Transplantation ; methods