Objective:The aim of this study was to investigate the mechanism of resveratrol activation of Sirt1/PGC-1α/Nrf2 pathway and its protective effect on apoptosis in rats with sepsis cardiomyopathy.Methods:In this study, 32 SD male rats were randomly (random number) divided into sham operation group (sham group), sepsis group (C group), Sirt1 agonist group (S group), Sirt1 agonist +PGC-1α inhibitor group (SP group), and cecal exploration was performed in rats of Sham group. The other three groups underwent Cecal ligation and puncture (CLP) to establish a rat model of septic cardiomyopathy, and the corresponding groups were given Sirt1 agonist resveratrol and PGC-1α inhibitor SR-18292. The heart of the rats were examined by B ultrasound 20 hours after CLP to compare left ventricular end-diastolic volume (LVEDV), left ventricular ejection fraction (LVEF), left ventricular end-systolic volume (LVESV) and left ventricular fraction shortening rate (LVFS). Myocardial tissue was stained with HE to observe the pathological changes, and serum myocardial troponin I (cTnI), interleukin-1β (IL-1β), interleukin-18 (IL-18) and oxidative stress indexes such as ROS and MDA were detected by ELISA. The expression levels of Sirt1, PGC-1α, Nrf2 and pyro related proteins (GSDMD, Caspase-1, NLRP3) in myocardial tissue were detected by Immunohistochemistry and Western Blotting.Results:Immunohistochemical and Western Blotting experiments showed that compared with sham group, the protein expressions of Sirt1, PGC-1α and Nrf2 in C group were decreased ( P < 0.05), and the expressions of GSDMD, Caspase-1 and NLRP3 were increased. The LVEF of group S rats was higher than that of group C rats ( P<0.05); Although the LVEDV and LVFS of group S were higher and the LVESV was lower, there was no statistically significant difference ( P > 0.05). Compared with group C, the edema and necrosis of cardiomyocytes in group S were alleviated, the arrangement of cardiomyocytes was slightly regular, and the inflammatory infiltration was reduced by HE staining. The cTnI, IL-1 β, IL-18, MDA, and ROS levels in group S were lower than those in group C ( P<0.05). Immunohistochemical and Western Blotting experiments showed that compared with group C, the protein expressions of Sirt1, PGC-1α and Nrf2 in group S were increased ( P < 0.05), and the expressions of GSDMD, Caspase-1 and NLRP3 were decreased ( P < 0.05). The LVESV of the SP group was higher than that of the S group rats ( P<0.05), while LVEDV, LVFS, and LVEF were all lower ( P>0.05). HE staining showed that the edema and necrosis of cardiomyocytes in SP group were more severe than those in S group, the arrangement of cardiomyocytes was more disordered than that in the former group, the vacuol-like deformation of cardiomyocytes was increased, and the infiltration of interstitial inflammatory cells was increased. The cTnI, IL-1 β, IL-18, MDA, and ROS levels in the SP group were higher than those in the S group rats ( P<0.05). There was no significant difference in Sirt1 expression between the SP group and the S group in myocardial tissue detected by immunohistochemistry and Western Blot. However, the expression levels of PGC-1 α and Nrf2 were lower in the SP group, while the expression levels of NLRP3, GSDMD, and Caspase-1 were higher in the SP group (all P<0.05). Conclusion:Resveratrol enhances myocardial function and decreases inflammatory cytokine levels in rats suffering from septic cardiomyopathy, potentially through a mechanism involving the Sirt1/PGC-1α/Nrf2 pathway.

Objective To get the information about the geriatric syndromes in the elderly with different age and gender so as to provide the basis for management of the geriatric syndromes.Methods A questionnaire survey was used to investigate and analyze the geriatric syndromes in 2 778 older subjects aged 60 years and above from Zhejiang and Jiangsu province.Results Among all 2 778 old adults,(3.4 ± 1.8)geriatric syndromes in average were found.The difference in geriatric syndromes number between different age groups showed a statistical significance(F =145.135,P＜ 0.001),but a significant difference in geriatric syndromes number between male and female group was not found(t=0.817,P=0.414).On the top incidence rate of geriatric syndromes were daily life ability defects 2 199 cases(79.2％),falls 1 828 cases(65.8％),urinary incontinence 1 356 cases(48.8％),sleeping disorder 1 023 cases(36.8 ％),cognitive impairment 908 cases (32.7 ％) and visual impairment 896 cases (32.3 ％).Among above six geriatric syndromes,the incidence of daily life ability defects,falls,urinary incontinence,sleeping disorder and cognitive impairment seemed to increase with ageing (P＜0.001).The incidence of daily life ability defects,falls and urinary incontinence was higher in males than in females (P＜ 0.05).The incidence of sleeping disorder and cognitive impairment was higher in females than in males(P＜0.05).The elderly patients with 3 or more geriatric syndromes exceeded about 66.4 ％.The most common clusters of geriatric syndromes were daily life ability defects combined with falls and urinary incontinence,which seemed to significantly increase along with ageing (x2=202.523,P＜0.001).Conclusions Geriatric syndromes are common in the elderly,medical care workers should pay attention to the management of the geriatric syndromes,and promote the development of the elderly healthcare.