Objective:To explore the clinical and pathological features and survival outcomes of patients with early-onset intrahepatic cholangiocarcinoma (EOICC).Methods:This is a multicenter, retrospective cohort study. Data of 1 160 intrahepatic cholangiocarcinoma patients undergoing radical resection in 14 tertiary Grade A hospitals in China from January 2010 to November 2021 were retrospectively collected. The cohort included 632 males and 528 females, aged( M (IQR)) 61 (14) years (range: 22 to 93 years). ICC aged ≤50 years at the time of diagnosis was defined as EOICC and >50 years as late-onset intrahepatic cholangiocarcinoma (LOICC). Of these, there were 247 cases in the EOICC group and 913 cases in the LOICC. The clinical and pathological characteristics of both groups were analyzed and compared using the independent sample t-test, Mann-Whitney U test or Kaplan-Meier method. Univariate and multivariate Cox regression models for patient outcomes were constructed and forest graphed. Results:Compared with the patients in the LOICC group, patients in the EOICC group had lower carcinoembryonic antigen levels (2.5(4.0) μg/L vs. 3.1(5.2)μg/L, U=124 899, P=0.009) and CA19-9 level (63.4(524.7)U/ml vs. 77.9(611.3)U/ml, U=120 320, P=0.013), higher levels of ALT (29(35)U/L vs. 24(26)U/L, U=101 214, P=0.013), a lower score of the Eastern US Cooperative Oncology Group (0 score patients: 54.7% vs. 44.1%, χ2=12.472, P=0.014), higher TNM stage ( χ2=11.807, P=0.038), and proportion of lymph node dissection (62.3% vs. 54.1%, χ2=5.355, P=0.021). Patients in the two groups in sex, first diagnosis symptoms, intrahepatic bile duct stone history, nail protein, albumin, total bilirubin, transaminase, liver function Child-Pugh grade, T stage, stage, N stage, preoperative laparoscopic exploration proportion, tumor diameter, vascular invasion proportion, differentiation, margin, intraoperative bleeding, postoperative complications, postoperative hospital days were no statistical significance (all P>0.05). Patients in the EOICC group had better outcomes than the LOICC group (median survival time: 29.7 months vs. 25.0 months, 3-year overall survival: 45.1% vs. 37.8%, P=0.027). Conclusion:EOICC patients are better than LOICC patients in carcinoembryonic antigen, CA19-9, ALT, physical strength status and TNM stage, and the long-term prognosis is also better than LOICC patients.

Objective To investigate the differential expression genes(DEGs)related to angiogenesis in rosacea(RA)by utilizing bioinformatics analysis in order to screen the key genes and verify their mRNA expression levels.Methods The gene microarray dataset GSE65914 was retrieved from the Gene Expression Omnibus(GEO)repository.Analyzed by R programming,the dataset was refined to identify DEGs related to RA,and then cross-referenced with angiogenesis-related genes from the GeneCards database to get a subset specific to RA angiogenesis.The process of identifying key genes was augmented by employing protein-protein interaction(PPI)network analysis and Cytoscape-based computational algorithms.The mRNA expression levels of the aforementioned pivotal genes were detected by real-time fluorescent quantitative reverse transcription PCR(RT-qPCR).Results A total of 947 RA-associated DEGs were identified from GEO dataset,and then 202 genes related to RA angiogenesis were further delineated.PPI network analysis and Cytoscape algorithm finally identified 3 key genes,that is,CXCL8,IL-1B,and STAT1.The results of RT-qPCR showed that the mRNA expression levels of MIP-2,GCP-2,IL-1B and STAT1 in RA lesions were significantly higher than those in normal controls(P<0.05).Conclusion With aid of bioinformatics analysis,our study has screened and validated key genes associated with angiogenesis in RA,namely CXCL8,IL-1B,and STAT1,which providing a theoretical basis for elucidating the potential mechanisms underlying RA-induced angiogenesis and developing targeted therapeutic strategies.

Objective:To investigate the current status of diagnostic X-ray resources in Jinan in order to provide theoretical basis for continuously optimizing the allocation of diagnostic resources and strengthening the management of radiological health and radiation protection.Methods:In accordance with the 2023 Jinan radiation protection monitoring program of medical and health institutions, a survey was carried out for medical institutions involved in diagnostic X-ray examinations (excluding military hospitals and dental clinics) in Jinan by using uniform questionnaires. The survey included the basic situation of diagnostic X-ray institutions, the allocation of diagnostic X-ray equipment, the number of diagnostic staff, and the frequency of diagnositic X-ray examinations.Results:In 2023, there were 298 diagnostic X-ray institutions in Jinan, with 3 494 diagnostic workers and 1 387 items of diagnostic X-ray equipment. The included were 41 tertiary hospitals, 57 secondary hospitals, 110 primary hospitals and 90 unrated hospitals. The tertiary hospitals possessed the largest number of the diagnostic workers and equipment, accounting for 60.99% and 45.35% of the total, respectively. Among all the items of diagnostic X-ray equipment, the number of items of digital radiography equipment ranked top (34.37%), followed by CT machines (24.44%). There were 147.32 items of diagnostic X-ray equipment per million population on average. A total of 8 180 363 individuals received various types of diagnostic X-ray examinations, with CT diagnosis accounting for 46.93% and X-ray for 34.41%. The frequency of diagnostic X-ray examinations was 868.86/1 000 population, including the frequency of CT examinations of 407.76 per 1 000 population.Conclusions:The per capita possession of medical X-ray equipment in Jinan is at a high level. The frequency of medical exposure has increased significantly, especially CT examinations. There is a big gap in diagnostic X-ray resource allocation between different levels of hospitals, so it is necessary to control the resource allocation and strengthen the protection of CT examination on the scientific basis.

Objective To explore the therapeutic effect of doubling the first dose of batroxobin in the treatment of sudden sensorineural hearing loss with reduced frequency.Methods A total of 96 patients diagnosed with sudden sensorineural hearing loss,who received treatment at the Department of Otolaryngology at the Second Affiliated Hospital of Guizhou Medical University between August 2022 and October 2024,were included in the study.Using the random number table method,the patients were assigned to two groups,an observation group and a control group,each comprising 48 cases.The control group was treated with Ginkgo biloba extract,methylpred-nisolone tablets,and a first dose of 10 BU of batroxobin,while the observation group was treated with Ginkgo biloba extract,methylprednisolone tablets,and a first dose of 20 BU of batroxobin.After one cycle of treatment,compare the pure tone hearing threshold,hemorheological indicators,coagulation function indicators,efficacy,and adverse reactions between the two groups.Follow up for 6 months and record the recurrence outcome.Results After treatment,the pure tone hearing threshold of both groups decreased,and the observation group was lower than the control group(P<0.05).After treatment,the effective rates of the observation group and the control group were 77.08%and 54.17%,respectively,and the whole blood low shear viscosity was(18.27±1.63)(20.29±1.41)mPa·s,the whole blood shear viscosity was(5.58±0.64),(6.01±0.69)mPa·s,the whole blood high shear viscosity is(4.26±0.38),(4.54±0.31)mPa·s,the plasma viscosity is(1.52±0.26),(1.71±0.34)mPa·s,the red blood cell aggregation index is(0.60±0.21),(0.52±0.17),the red blood cell deformation index was(5.73±0.61),(6.20±0.64),and the fibrinogen(FIB)is(0.72±0.18).0.95±0.13)g/L,thrombin time(TT)were(23.51±2.08)and(21.76±2.13)s,prothrombin time(PT)were(12.71±0.63)and(12.05±0.65)s,respectively,and activated partial thromboplastin time(APTT)were(28.32±3.07)and(29.51±2.24)s,respectively,with the observation group showing more significant changes(P<0.05).The recurrence rate of the observation group was lower than that of the control group(P<0.05).Conclusion Doubling the first dose of batroxobin can help alleviate the symptoms of hearing impairment,improve inner ear microcirculation,and enhance treatment efficacy in patients with total frequency decline type sudden deafness.

Objective To explore the effects of oleanolic acid on NF-κB/caspase-9 signaling pathway in kidneys of rat with diabetic nephropathy.Methods The diabetic nephropathy model rats were established,and the model rats were randomly divided intooleanolic acid low,medium and high dose groups,metformin group,and model group,with another 12 healthy SD rats as control group.The levels of blood glucose were measured at weeks 1,2 and 3 post-drug administration,and blood lipid and 24h urine urinary microalbumin(UMA)were measured after entire drug administration.Furthermore,we also detected the renal histopathology of rats,apoptosis of renal tubular,glomerular cells,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and protein expression of NF-κB/caspase-9 signaling pathway.Results Compared with the control group,the model group demonstrated higher levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,higher proportion of apoptotic renal tubular and glomerular,and higher expressions of caspase-9 protein and p-NF-κB p65/NF-κB p65(P＜0.05).Compared with the model group,the pathological damage of renal tissue in the metformin group and oleanolic acid low,middle and high dose groups were alleviated,the levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,the proportion of apoptotic renal tubular and glomerular,and the expressions of caspase-9 proteins and p-NF-κB p65/NF-κB p65 were decreased,and these indexes in oleanolic acid groups showed a dose-dependent manner(P＜0.05).Conclusion Oleanolic acid can improve the metabolism of glucose and lipid,reduce the pathological damage of renal tissue,inhibit the apoptosis of renal tubular and glomerular,and inhibit the NF-κB/caspase-9 pathway in diabetic nephropathy rats.

Objective To investigate and analyze the resources and application frequency of radiological diagnosis and treatment in Jinan City in 2023 and provide a basis for the rational application of radiological diagnosis and treatment resources and strengthening radiological health protection management. Methods The health administrative department issued a work plan. A general survey was conducted on radiological diagnosis and treatment institutions (excluding dental clinics) in Jinan City using a questionnaire. The survey covered the basic information of the radiological diagnosis and treatment institutions, the distribution of the radiological diagnosis and treatment equipment, the number of radiological workers, and the frequency of radiological diagnosis and treatment. Results There were 301 radiological diagnosis and treatment institutions in Jinan City, with 1603 sets of radiological diagnosis and treatment equipment and 5789 radiological workers. These institutions included 41 (13.62%) tertiary hospitals, 57 (18.94%) secondary hospitals, 110 (36.54%) primary hospitals, and 93 (30.90%) ungraded hospitals. Tertiary hospitals had the highest proportions of radiological diagnosis and treatment equipment and workers, accounting for 49.53% and 69.10% of the total, respectively. The frequencies of radiological diagnosis and treatment were 868.86 X-ray diagnostic imaging examinations per 1000 population, 5.07 radiotherapies per 1000 population, 14.19 interventional diagnostic and therapeutic procedures per 1000 population, 7.36 nuclear medical diagnostic procedures per 1000 population, and 0.56 nuclear medical therapeutic procedures per 1000 population. The frequency of CT diagnosis was the highest, reaching 407.76 procedures per 1000 population. Conclusion The radiological diagnosis and treatment resources and activities in Jinan City were mainly concentrated in tertiary hospitals, with unbalanced distribution among hospitals of different grades. It is necessary to apply various medical radiations correctly and reasonably and strengthen radiation protection to reduce the frequency of radiation and the collective effective dose.

Objective To explore the therapeutic effect of doubling the first dose of batroxobin in the treatment of sudden sensorineural hearing loss with reduced frequency.Methods A total of 96 patients diagnosed with sudden sensorineural hearing loss,who received treatment at the Department of Otolaryngology at the Second Affiliated Hospital of Guizhou Medical University between August 2022 and October 2024,were included in the study.Using the random number table method,the patients were assigned to two groups,an observation group and a control group,each comprising 48 cases.The control group was treated with Ginkgo biloba extract,methylpred-nisolone tablets,and a first dose of 10 BU of batroxobin,while the observation group was treated with Ginkgo biloba extract,methylprednisolone tablets,and a first dose of 20 BU of batroxobin.After one cycle of treatment,compare the pure tone hearing threshold,hemorheological indicators,coagulation function indicators,efficacy,and adverse reactions between the two groups.Follow up for 6 months and record the recurrence outcome.Results After treatment,the pure tone hearing threshold of both groups decreased,and the observation group was lower than the control group(P<0.05).After treatment,the effective rates of the observation group and the control group were 77.08%and 54.17%,respectively,and the whole blood low shear viscosity was(18.27±1.63)(20.29±1.41)mPa·s,the whole blood shear viscosity was(5.58±0.64),(6.01±0.69)mPa·s,the whole blood high shear viscosity is(4.26±0.38),(4.54±0.31)mPa·s,the plasma viscosity is(1.52±0.26),(1.71±0.34)mPa·s,the red blood cell aggregation index is(0.60±0.21),(0.52±0.17),the red blood cell deformation index was(5.73±0.61),(6.20±0.64),and the fibrinogen(FIB)is(0.72±0.18).0.95±0.13)g/L,thrombin time(TT)were(23.51±2.08)and(21.76±2.13)s,prothrombin time(PT)were(12.71±0.63)and(12.05±0.65)s,respectively,and activated partial thromboplastin time(APTT)were(28.32±3.07)and(29.51±2.24)s,respectively,with the observation group showing more significant changes(P<0.05).The recurrence rate of the observation group was lower than that of the control group(P<0.05).Conclusion Doubling the first dose of batroxobin can help alleviate the symptoms of hearing impairment,improve inner ear microcirculation,and enhance treatment efficacy in patients with total frequency decline type sudden deafness.

Objective To explore the effects of oleanolic acid on NF-κB/caspase-9 signaling pathway in kidneys of rat with diabetic nephropathy.Methods The diabetic nephropathy model rats were established,and the model rats were randomly divided intooleanolic acid low,medium and high dose groups,metformin group,and model group,with another 12 healthy SD rats as control group.The levels of blood glucose were measured at weeks 1,2 and 3 post-drug administration,and blood lipid and 24h urine urinary microalbumin(UMA)were measured after entire drug administration.Furthermore,we also detected the renal histopathology of rats,apoptosis of renal tubular,glomerular cells,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and protein expression of NF-κB/caspase-9 signaling pathway.Results Compared with the control group,the model group demonstrated higher levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,higher proportion of apoptotic renal tubular and glomerular,and higher expressions of caspase-9 protein and p-NF-κB p65/NF-κB p65(P＜0.05).Compared with the model group,the pathological damage of renal tissue in the metformin group and oleanolic acid low,middle and high dose groups were alleviated,the levels of blood glucose at weeks 1,2 and 3,blood lipid,UMA,TNF-α and IL-6,the proportion of apoptotic renal tubular and glomerular,and the expressions of caspase-9 proteins and p-NF-κB p65/NF-κB p65 were decreased,and these indexes in oleanolic acid groups showed a dose-dependent manner(P＜0.05).Conclusion Oleanolic acid can improve the metabolism of glucose and lipid,reduce the pathological damage of renal tissue,inhibit the apoptosis of renal tubular and glomerular,and inhibit the NF-κB/caspase-9 pathway in diabetic nephropathy rats.

Objective:To investigate the current status of diagnostic X-ray resources in Jinan in order to provide theoretical basis for continuously optimizing the allocation of diagnostic resources and strengthening the management of radiological health and radiation protection.Methods:In accordance with the 2023 Jinan radiation protection monitoring program of medical and health institutions, a survey was carried out for medical institutions involved in diagnostic X-ray examinations (excluding military hospitals and dental clinics) in Jinan by using uniform questionnaires. The survey included the basic situation of diagnostic X-ray institutions, the allocation of diagnostic X-ray equipment, the number of diagnostic staff, and the frequency of diagnositic X-ray examinations.Results:In 2023, there were 298 diagnostic X-ray institutions in Jinan, with 3 494 diagnostic workers and 1 387 items of diagnostic X-ray equipment. The included were 41 tertiary hospitals, 57 secondary hospitals, 110 primary hospitals and 90 unrated hospitals. The tertiary hospitals possessed the largest number of the diagnostic workers and equipment, accounting for 60.99% and 45.35% of the total, respectively. Among all the items of diagnostic X-ray equipment, the number of items of digital radiography equipment ranked top (34.37%), followed by CT machines (24.44%). There were 147.32 items of diagnostic X-ray equipment per million population on average. A total of 8 180 363 individuals received various types of diagnostic X-ray examinations, with CT diagnosis accounting for 46.93% and X-ray for 34.41%. The frequency of diagnostic X-ray examinations was 868.86/1 000 population, including the frequency of CT examinations of 407.76 per 1 000 population.Conclusions:The per capita possession of medical X-ray equipment in Jinan is at a high level. The frequency of medical exposure has increased significantly, especially CT examinations. There is a big gap in diagnostic X-ray resource allocation between different levels of hospitals, so it is necessary to control the resource allocation and strengthen the protection of CT examination on the scientific basis.

Objective:To explore the clinical and pathological features and survival outcomes of patients with early-onset intrahepatic cholangiocarcinoma (EOICC).Methods:This is a multicenter, retrospective cohort study. Data of 1 160 intrahepatic cholangiocarcinoma patients undergoing radical resection in 14 tertiary Grade A hospitals in China from January 2010 to November 2021 were retrospectively collected. The cohort included 632 males and 528 females, aged( M (IQR)) 61 (14) years (range: 22 to 93 years). ICC aged ≤50 years at the time of diagnosis was defined as EOICC and >50 years as late-onset intrahepatic cholangiocarcinoma (LOICC). Of these, there were 247 cases in the EOICC group and 913 cases in the LOICC. The clinical and pathological characteristics of both groups were analyzed and compared using the independent sample t-test, Mann-Whitney U test or Kaplan-Meier method. Univariate and multivariate Cox regression models for patient outcomes were constructed and forest graphed. Results:Compared with the patients in the LOICC group, patients in the EOICC group had lower carcinoembryonic antigen levels (2.5(4.0) μg/L vs. 3.1(5.2)μg/L, U=124 899, P=0.009) and CA19-9 level (63.4(524.7)U/ml vs. 77.9(611.3)U/ml, U=120 320, P=0.013), higher levels of ALT (29(35)U/L vs. 24(26)U/L, U=101 214, P=0.013), a lower score of the Eastern US Cooperative Oncology Group (0 score patients: 54.7% vs. 44.1%, χ2=12.472, P=0.014), higher TNM stage ( χ2=11.807, P=0.038), and proportion of lymph node dissection (62.3% vs. 54.1%, χ2=5.355, P=0.021). Patients in the two groups in sex, first diagnosis symptoms, intrahepatic bile duct stone history, nail protein, albumin, total bilirubin, transaminase, liver function Child-Pugh grade, T stage, stage, N stage, preoperative laparoscopic exploration proportion, tumor diameter, vascular invasion proportion, differentiation, margin, intraoperative bleeding, postoperative complications, postoperative hospital days were no statistical significance (all P>0.05). Patients in the EOICC group had better outcomes than the LOICC group (median survival time: 29.7 months vs. 25.0 months, 3-year overall survival: 45.1% vs. 37.8%, P=0.027). Conclusion:EOICC patients are better than LOICC patients in carcinoembryonic antigen, CA19-9, ALT, physical strength status and TNM stage, and the long-term prognosis is also better than LOICC patients.