Objective To investigate the correlation between urinary β2-microglobulin(β2-MG)and the clinicopathological characteristics of IgA nephropathy(IgAN).Methods A retrospective analysis was conducted on 77 patients diagnosed with IgAN by renal biopsy from October 2019 to October 2024.The research group was defined as those with a urinary β2-MG value higher than or equal to the median,and the control group was defined as those with a value lower than the median.The clinicopathological indicators of the two groups were compared.The Spearman method was used to analyze the correlation of the estimated glomerular filtration rate(eGFR)and pathological indicators of IgAN patients with urinary β2-MG.Multivariate logistic regression was used to analyze the clinicopathological factors related to urinary β2-MG.The receiver operating characteristic(ROC)curve and the area under the curve(AUC)were used to evaluate the ability of urinary β2-MG to predict related pathological damage.Result Compared with the control group,the research group had older age,elevated levels of serum creatinine and blood urea,increased 24-hour urine protein quantification,elevated levels of urine α 1-microglobulin and urinary β2-MG,elevated urine microalbumin,elevated levels of total cholesterol and D-dimer,and decreased levels of eGFR,serum albumin,hemoglobin,and complement C3(P＜0.05).Compared with the control group,the research group had more severe pathological damage in terms of renal tubular atrophy and renal interstitial fibrosis(T),as well as cellular and fibrocellular crescents(C)(P＜0.05).There was a negative correlation between the urinary β2-MG level and eGFR in patients with IgAN(P＜0.01),and a significant positive correlation with T and C(P＜0.05).After controlling for confounding factors,eGFR(OR=0.983),serum albumin(OR=0.889),triglycerides(OR=0.099),total cholesterol(OR=2.677),and pathological damage T(OR=11.358)and C(OR=12.018)were independent influencing factors associated with high urinary β2-MG levels(P＜0.05).The AUC of urinary β2-MG level for predicting the pathological indicator T in patients with IgAN was 0.784(95％CI:0.660,0.907),with the corresponding sensitivity of 0.717,and the specificity of 0.882.Conclusion The level of urinary β2-MG is closely related to multiple clinicopathological characteristics of patients with IgAN.It can be used as a potential biomarker for evaluating renal structural damage in patients,especially tubulointerstitial and renal vascular lesions,and has a certain predictive ability for tubulointerstitial injury.

Objective To investigate the correlation between urinary β2-microglobulin(β2-MG)and the clinicopathological characteristics of IgA nephropathy(IgAN).Methods A retrospective analysis was conducted on 77 patients diagnosed with IgAN by renal biopsy from October 2019 to October 2024.The research group was defined as those with a urinary β2-MG value higher than or equal to the median,and the control group was defined as those with a value lower than the median.The clinicopathological indicators of the two groups were compared.The Spearman method was used to analyze the correlation of the estimated glomerular filtration rate(eGFR)and pathological indicators of IgAN patients with urinary β2-MG.Multivariate logistic regression was used to analyze the clinicopathological factors related to urinary β2-MG.The receiver operating characteristic(ROC)curve and the area under the curve(AUC)were used to evaluate the ability of urinary β2-MG to predict related pathological damage.Result Compared with the control group,the research group had older age,elevated levels of serum creatinine and blood urea,increased 24-hour urine protein quantification,elevated levels of urine α 1-microglobulin and urinary β2-MG,elevated urine microalbumin,elevated levels of total cholesterol and D-dimer,and decreased levels of eGFR,serum albumin,hemoglobin,and complement C3(P＜0.05).Compared with the control group,the research group had more severe pathological damage in terms of renal tubular atrophy and renal interstitial fibrosis(T),as well as cellular and fibrocellular crescents(C)(P＜0.05).There was a negative correlation between the urinary β2-MG level and eGFR in patients with IgAN(P＜0.01),and a significant positive correlation with T and C(P＜0.05).After controlling for confounding factors,eGFR(OR=0.983),serum albumin(OR=0.889),triglycerides(OR=0.099),total cholesterol(OR=2.677),and pathological damage T(OR=11.358)and C(OR=12.018)were independent influencing factors associated with high urinary β2-MG levels(P＜0.05).The AUC of urinary β2-MG level for predicting the pathological indicator T in patients with IgAN was 0.784(95％CI:0.660,0.907),with the corresponding sensitivity of 0.717,and the specificity of 0.882.Conclusion The level of urinary β2-MG is closely related to multiple clinicopathological characteristics of patients with IgAN.It can be used as a potential biomarker for evaluating renal structural damage in patients,especially tubulointerstitial and renal vascular lesions,and has a certain predictive ability for tubulointerstitial injury.

Objective To study the effect and mechanism of high glucose on mesothelial-mesenchymal transition(MMT)of peritoneal mesothelial cells(HMrSV5),and the protective effect of pharmacological blocking of signal transducer and activator of transcription 3(STAT3)on rat peritoneal fibrosis(PF)model.Methods The animals were divided into three groups:the sham group,the model group,and the STAT3 inhibitor group.A miniature per-itoneal dialysis catheter was implanted under the dorsal skin of rat and the rat peritoneal fibrosis model was induced by daily injection of high glucose dialysate.After 10 weeks,HE staining was used to evaluate the histology of the peritoneum,and the level of transforming growth factor-β1(TGF-β1)in the peritoneum was measured by immuno-histochemistry.HMrSV5 was cultured in high glucose and the optimal stimulation concentration of high glucose was determined by Western blot.High glucose was used to stimulate HMrSV5 after successful transfection with si-STAT3 and Western blot was used to measure the protein level of STAT3,p-STAT3,and the key enzymes of glycol-ysis 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3(PFKFB3)and lactate dehydrogenase A(LDHA).Results HE staining showed that administration of STAT3 inhibitor(BP-1-102)could inhibit the thickening of subperitoneal tissue and the proliferation of vessels in HG dialysis rats.The expression of TGF-β1 in the rats perito-neum of the model group was significantly higher than that in the sham group,and the level of TGF-β1 was marked-ly lower in the STAT3 inhibitor group compared to the model group(P<0.05).Compared to the control group,high glucose induced the up-regulation of α-smooth muscle actin(α-SMA),the down-regulation of E-cadherin and STAT3 activation in HMrSV5(P<0.05).Mesothelial cells treated with high glucose also exhibited high expres-sion of the key enzymes of glycolysis(PFKFB3,LDHA)(P<0.05),and si-STAT3 can effectively inhibit the overexpression of PFKFB3 and LDHA induced by high glucose(P<0.05).Conclusion STAT3 is involved in high glucose-induced HMrSV5 hyperglycolysis and MMT,and targeting STAT3 alleviates peritoneal fibrosis and an-giogenesis during peritoneal dialysis treatment in rats.