Objective:To investigate the effect of colonoscopy on intestinal microecology in healthy adults of different ages and its therapeutic strategy.Methods:A total of 128 healthy officers and soldiers of different ages and retired military personnel who underwent physical examination in the Outpatient Department of Chinese PLA Medical School from May 1, 2020 to December 30, 2020 were selected as the research subjects. They were divided into young people group (Group Y, 18 to 35 years old, n=37), middle-aged people group (Group M, 36 to 59 years old, n=44), and elderly people group (Group E, 60 years old or above, n=47) according to their age. Another 110 elderly research subjects were divided into placebo group (Control Group, n=36), Brewer’s yeast group (Yeast Group, n=35) and Bacillus subtilis combined with Enterococcus faecium group (Combined Group, n=39) according to the treatment methods. Fecal samples were collected 1 day before intestinal preparation for colonoscopy, 7 days, and 14 days after colonoscopy, respectively, and the abundance, diversity, and composition of intestinal microflora were detected by 16S rRNA sequencing technology. Steady-state changes and adverse symptoms of intestinal microflora in the elderly research subjects after treatment were observed. Results:Before intestinal preparation, there was no statistically significant difference among the three age groups in the abundance of intestinal microflora ( P>0.05); on the 7th day after colonoscopy, the abundance was all decreased significantly; on the 14th day after colonoscopy, the abundance of the Group Y and the Group M could recover to the levels before intestinal preparation, while that of the Group E was not completely recovered ( P<0.05). Compared with the 14th day after colonoscopy, there was no statistically significant difference in the diversity of intestinal microflora among the three age groups before the colonoscopy ( P>0.05). Compared with before intestinal preparation and the 14th day after colonoscopy, the diversity on the 7th day after colonoscopy was significantly decreased ( P<0.05). Regarding the phylum level, there was no statistically significant difference in the composition of microflora between the Group Y and the Group M before intestinal preparation ( P>0.05). The proportion of Firmicutes in the Group E was slightly lower than those in the Groups Y and M, while the proportion of Bacteroides was higher ( P<0.05). Regarding the family level, compared with the 14th day after colonoscopy, there was no statistically significant difference in the composition of bacteria in the Groups Y and M before intestinal preparation ( P>0.05). Compared with before intestinal preparation, the numbers of trichospiraceae in the three age groups on the 7th day after colonoscopy were all significantly decreased ( P<0.05), while the numbers of enterobacteriaceae were significantly increased ( P<0.05). The abundance of Lactobacillus in the Group E was higher than those in the Groups Y and M at three time points ( P<0.05). The abundance of intestinal microflora in the Yeast Group and the Combined Group were significantly higher than that of the Control Group. The clustering trends of the Yeast Group, the Combined Group, and the Control Group were significantly different at different treatment stages. The incidence of adverse symptoms in the Combined Group and the Yeast Group was significantly lower than that of the Control group ( P<0.05). Conclusion:Colonoscopy can cause imbalance of intestinal microbiota homeostasis, and the elderly have a longer recovery time and suffer from more complications. Probiotics can help them quickly recover intestinal microbiota homeostasis and reduce the incidence of complications.

Objective:To investigate the effect of colonoscopy on intestinal microecology in healthy adults of different ages and its therapeutic strategy.Methods:A total of 128 healthy officers and soldiers of different ages and retired military personnel who underwent physical examination in the Outpatient Department of Chinese PLA Medical School from May 1, 2020 to December 30, 2020 were selected as the research subjects. They were divided into young people group (Group Y, 18 to 35 years old, n=37), middle-aged people group (Group M, 36 to 59 years old, n=44), and elderly people group (Group E, 60 years old or above, n=47) according to their age. Another 110 elderly research subjects were divided into placebo group (Control Group, n=36), Brewer’s yeast group (Yeast Group, n=35) and Bacillus subtilis combined with Enterococcus faecium group (Combined Group, n=39) according to the treatment methods. Fecal samples were collected 1 day before intestinal preparation for colonoscopy, 7 days, and 14 days after colonoscopy, respectively, and the abundance, diversity, and composition of intestinal microflora were detected by 16S rRNA sequencing technology. Steady-state changes and adverse symptoms of intestinal microflora in the elderly research subjects after treatment were observed. Results:Before intestinal preparation, there was no statistically significant difference among the three age groups in the abundance of intestinal microflora ( P>0.05); on the 7th day after colonoscopy, the abundance was all decreased significantly; on the 14th day after colonoscopy, the abundance of the Group Y and the Group M could recover to the levels before intestinal preparation, while that of the Group E was not completely recovered ( P<0.05). Compared with the 14th day after colonoscopy, there was no statistically significant difference in the diversity of intestinal microflora among the three age groups before the colonoscopy ( P>0.05). Compared with before intestinal preparation and the 14th day after colonoscopy, the diversity on the 7th day after colonoscopy was significantly decreased ( P<0.05). Regarding the phylum level, there was no statistically significant difference in the composition of microflora between the Group Y and the Group M before intestinal preparation ( P>0.05). The proportion of Firmicutes in the Group E was slightly lower than those in the Groups Y and M, while the proportion of Bacteroides was higher ( P<0.05). Regarding the family level, compared with the 14th day after colonoscopy, there was no statistically significant difference in the composition of bacteria in the Groups Y and M before intestinal preparation ( P>0.05). Compared with before intestinal preparation, the numbers of trichospiraceae in the three age groups on the 7th day after colonoscopy were all significantly decreased ( P<0.05), while the numbers of enterobacteriaceae were significantly increased ( P<0.05). The abundance of Lactobacillus in the Group E was higher than those in the Groups Y and M at three time points ( P<0.05). The abundance of intestinal microflora in the Yeast Group and the Combined Group were significantly higher than that of the Control Group. The clustering trends of the Yeast Group, the Combined Group, and the Control Group were significantly different at different treatment stages. The incidence of adverse symptoms in the Combined Group and the Yeast Group was significantly lower than that of the Control group ( P<0.05). Conclusion:Colonoscopy can cause imbalance of intestinal microbiota homeostasis, and the elderly have a longer recovery time and suffer from more complications. Probiotics can help them quickly recover intestinal microbiota homeostasis and reduce the incidence of complications.

Objective To study the therapeutic effects and related mechanism of Tongmai Yizhi granules on vascular dementia in rats. Methods Bilateral carotid artery ligation was used to establish rat model of vascular dementia (VD). The rats were randomly divided into 6 groups: sham operated,the model control,piracetam (0. 375 g·kg-1 ·d-1 ) as positive con-trol,low dose (2. 5 g·kg-1 ·d-1 ),middle dose(5. 0 g·kg-1 ·d-1 ),and high dose (10. 0 g·kg-1 ·d-1 ) of Tongmai Yizhi granules. Each group was intragastrically administered with 10 mL·kg-1 of corresponding medications for 31 days after the VD model was established. The sham group was given with 0. 9% NaCl solution. Y-electric maze was used to test the learning and memory function of rats at the second and fourth weeks. On the day 32,hippocampal tissues were collected for pathological analy-sis by microscope. The activities of SOD and content of MDA in serum and tissue homogenate were tested. Results Compared with model control group,each dose of Tongmai Yizhi granules obviously shortened the incubation period of VD rats in electric maze test,and accuracy of learning and memory was improved (P<0. 05 or P<0. 01). The activity of SOD was decreased,and the content of MDA was increased after treatment with Tongmai Yizhi granules (P < 0. 01). Hippocampal neuronal cells were disar-ranged,and the number of cone cells was decreased significantly in the model control group. In contrast,the necrosis and degener-ation of hippocampal neurons were alleviated in all Tongmai Yizhi treatment groups. Conclusion Tongmai Yizhi granule can apparently improve learning and memory function of VD rats. It can also improve the activity of SOD and reduce the content of MDA in serum and hippocampus,effectively remove free radicals,and alleviate the injury of hippocampal pyramidal cells.

Objective According to the changes of microcirculation in the pulmonary cancers, the treatment effect was evaluated after the vascular interventional therapy.Methods Angiography of 138 primary pulmonary carcinomas, and the feeding arteries were performed. Areas of mass blush were measured for 81 cases before and after therapy. The tumour blush was considered to be the imaging appearance of the microcirculation of the lung carcinoma. The angiographic images were collected by digital image system (DSA and movie). Results (1) The rate of the tumour blush appearance was 88.8% in this group. (2) The areas of the lung carcinoma blush in 81 cases before and after therapy were (941.4?73.2)mm 2 and (427.9? 93.8 )mm 2( P