Objective : To investigate the effect of overexpression of tyrosine kinase receptor 1 (Tie-1) in cervical cancer cells on the malignant biological behavior of tumor-related endothelial cells (TRECs) ． Methods : Immuno- histochemical method was used to detect the expression of Tie-1 in cervical cancer cells ( CCCs) and TRECs of 96 patients with cervical cancer，and to analyze the correlation between the expression of Tie-1 in TRECs and clinico- pathological features and prognosis of patients．HeLa cells overexpressing Tie-1 (Hela-Tie1OE) were constructed， and HeLa-Tie1OE was co-cultured with human umbilical vein endothelial cells ( HUVECs) by Transwell cell co- culture method to obtain cervical cancer TRECs．Western blot was used to analyze Tie-1 protein expression．The migration，invasion and tubulogenesis of TRECs were detected by cell scratch assay，Transwell invasion and migra- tion assay and tubulogenesis assay. Results : The expression of Tie-1 was positively correlated with CCCs and TRECs in 96 cervical cancer patients．The positive expression rate of Tie-1 in TRECs of patients with stage(FIGO) Ⅰ B2-ⅡA，tumor diameter ≥4 cm ，cervical muscle invasion depth ≥ 1 /2 full layer ，adenocarcinoma ，medium and low differentiation cervical cancer was higher than that of patients with Ⅰ A 1-Ⅰ B 1，tumor diameter＜4 cm， cervical muscle invasion depth ＜1 /2 full layer，squamous carcinoma，and high differentiation cervical cancer，re- spectively．The differences were statistically significant (P＜0. 05) ．The expression of Tie-1 in TRECs of cervical cancer patients had no significant correlation with age，lymph node metastasis and lymphatic space invasion．The positive expression of Tie-1 in cervical cancer TRECs was negatively correlated with 5-year progression-free survival time and overall survival time (P＜0. 05) ．The Tie-1 expression of TRECs obtained by co-culture of Hela-Tie1OE and HUVECs was up-regulated ，and the invasion ，migration and tubulogenesis of TRECs cells were enhanced. Conclusion The high expression of Tie-1 in TRECs of cervical cancer is related to FIGO stage，tumor diameter， degree of differentiation，depth of cervical muscular invasion，type of cervical cancer，and poor prognosis of pa- tients．Overexpression of Tie-1 can promote TRECs invasion，migration and tubulogenesis.