In vitro erythrocyte manufacturing technology offers a novel solution for transfusion therapy,en-suring sustainable blood supply,reducing dependence on donors,and minimizing the risk of infectious disease transmission.This review summarizes recent advancements in in vitro erythrocyte production,including techniques for generating red blood cells(RBC)from various cell sources,with a focus on the cultivation and development of immortalized erythroid cell lines.The importance of enhancing enucleation efficiency and ensuring the safety of immortalized erythroid cells is emphasized.Additionally,the potential and challenges in clinical translation are discussed,aiming to provide new insights for future sustainable RBC supply and therapeutic applications.

In vitro erythrocyte manufacturing technology offers a novel solution for transfusion therapy, ensuring sustainable blood supply, reducing dependence on donors, and minimizing the risk of infectious disease transmission. This review summarizes recent advancements in in vitro erythrocyte production, including techniques for generating red blood cells (RBC) from various cell sources, with a focus on the cultivation and development of immortalized erythroid cell lines. The importance of enhancing enucleation efficiency and ensuring the safety of immortalized erythroid cells is emphasized. Additionally, the potential and challenges in clinical translation are discussed, aiming to provide new insights for future sustainable RBC supply and therapeutic applications.

OBJECTIVE：To study the improvement effect and mechanism of MEBO on lipopolysaccharide （LPS）-induced injury of rat skin fibroblasts. METHODS ：Skin fibroblasts of rats were divided into control group ，LPS group （5 μg/mL）， Kangfuxin solution group （positive control ，5 μg/mL LPS+1.25％ Kangfuxin solution ）and MEBO group （5 μg/mL LPS+0.6 mg/mL MEBO），with 6 wells in each group. Inflammatory injury cell model was induced by LPS （except for control group ）. After a certain period of cultivation ，the cell survival rate and cell migration rate were detected in each group. The contents of TNF-α and IL-6 in cell supernatant was detected. The localization and fluorescence intensity of IL- 6 protein were detected. The protein expression of PTEN ，p-p65，TNF-α，IL-6，PI3K and Akt in the fibroblasts were also determined. RESULTS ：Compared with control group ，survival rate of the fibroblasts was increased significantly in LPS group ，while cell migration was decreased significantly；the contents of TNF-α and IL-6 in cell supernatant as well as relative protein expression of PTEN ，p-p65，TNF-α， IL-6 and PI 3K were increased significantly （P＜0.05 or P＜0.01）；IL-6 protein mainly expressed in the cytoplasm ，and the fluorescence intensity was enhanced. Compared with LPS group ，survival rate of the fibroblasts was decreased significantly in Kangfuxin solution group and MEBO group ，while migration rate was increased significantly ；the contents of TNF-α and IL-6， relative protein expression of PTEN ，p-p65，TNF-α，IL-6（except for Kangfuxin solution group ），PI3K and Akt （except for Kangfuxin solution group ） were decreased significantly （P＜0.05 or P＜0.01），while fluorescence intensity of IL- 6 protein decreased；relative protein expression of TNF-α，IL-6，PI3K and Akt in MEBO group were significantly lower than Kangfuxin solution group （P＜0.05 or P＜0.01）. CONCLUSIONS ：MEBO can inhibit the proliferation of LPS-induced skin fibroblasts ， reduce the level of inflammatory factors and the intensity of inflammatory reaction ， which may be related to the jiang- down-regulation of PTEN/NF-κB，PI3K/Akt signaling pathway.