BACKGROUND:Janus micro/nanoparticles are widely used in the field of tissue engineering,drug delivery,cancer therapy,bioimaging,and sensing due to their shape,structure,and functional anisotropy.OBJECTIVE:To elucidate the cutting-edge applications of Janus micro/nanoparticles in biomedicine.METHODS:Relevant literature published between 2010 and 2024 was retrieved from CNKI,WanFang Data,PubMed,and Web of Science databases.Searches were conducted using Chinese search terms"Janus nanoparticle,Janus particle,dual-faced particle,drug delivery,cancer therapy,bioimaging,biosensing,tissue engineering"and English search terms"Janus nanoparticle,Janus particle,drug delivery,cancer therapy,biosensing,bioimaging,tissue engineering."A total of 69 articles were selected for review after screening,organizing,summarizing,and synthesizing.RESULTS AND CONCLUSION:Janus micro/nanoparticles can be classified into three major categories based on their basic material properties:organic,inorganic,and organic-inorganic composites.Synthetic strategies consist of shielding,self-assembly,phase separation,microfluidics,and nucleation and growth techniques.Janus micro/nanoparticles exhibit high-efficiency drug delivery through characteristics such as high drug loading,gated release,and autonomous motion.In addition to enhancing traditional cancer treatment modalities(radiation and chemotherapy),Janus micro/nanoparticles can also be applied to emerging cancer treatment methods such as cell immunotherapy,protein drugs,and ferroptosis.Janus micro/nanoparticles serve as contrast agents to enhance bioimaging modalities(CT,MRI,and ultrasound)for high-quality imaging,guiding diagnosis and treatment.Janus micro/nanoparticles are utilized in tissue engineering for delivering growth factors,enhancing mechanical properties of biomaterials,and exhibiting antibacterial effects.Researchers have successfully tailored Janus micro/nanoparticles with desired functionalities by combining different organic polymers and inorganic materials using various synthetic strategies,enabling applications in complex biomedical fields.Despite current advancements,reports on the application of Janus micro/nanoparticles in tissue regeneration,large-scale production,and human clinical trials are relatively limited.Therefore,further research efforts are needed in the development,synthetic strategies,clinical safety assessment,and translation of such materials.

BACKGROUND:Janus micro/nanoparticles are widely used in the field of tissue engineering,drug delivery,cancer therapy,bioimaging,and sensing due to their shape,structure,and functional anisotropy.OBJECTIVE:To elucidate the cutting-edge applications of Janus micro/nanoparticles in biomedicine.METHODS:Relevant literature published between 2010 and 2024 was retrieved from CNKI,WanFang Data,PubMed,and Web of Science databases.Searches were conducted using Chinese search terms"Janus nanoparticle,Janus particle,dual-faced particle,drug delivery,cancer therapy,bioimaging,biosensing,tissue engineering"and English search terms"Janus nanoparticle,Janus particle,drug delivery,cancer therapy,biosensing,bioimaging,tissue engineering."A total of 69 articles were selected for review after screening,organizing,summarizing,and synthesizing.RESULTS AND CONCLUSION:Janus micro/nanoparticles can be classified into three major categories based on their basic material properties:organic,inorganic,and organic-inorganic composites.Synthetic strategies consist of shielding,self-assembly,phase separation,microfluidics,and nucleation and growth techniques.Janus micro/nanoparticles exhibit high-efficiency drug delivery through characteristics such as high drug loading,gated release,and autonomous motion.In addition to enhancing traditional cancer treatment modalities(radiation and chemotherapy),Janus micro/nanoparticles can also be applied to emerging cancer treatment methods such as cell immunotherapy,protein drugs,and ferroptosis.Janus micro/nanoparticles serve as contrast agents to enhance bioimaging modalities(CT,MRI,and ultrasound)for high-quality imaging,guiding diagnosis and treatment.Janus micro/nanoparticles are utilized in tissue engineering for delivering growth factors,enhancing mechanical properties of biomaterials,and exhibiting antibacterial effects.Researchers have successfully tailored Janus micro/nanoparticles with desired functionalities by combining different organic polymers and inorganic materials using various synthetic strategies,enabling applications in complex biomedical fields.Despite current advancements,reports on the application of Janus micro/nanoparticles in tissue regeneration,large-scale production,and human clinical trials are relatively limited.Therefore,further research efforts are needed in the development,synthetic strategies,clinical safety assessment,and translation of such materials.

Objective:To explore the application of GLTC (goodwill, listening, talking, and cooperation) doctor-patient communication mode combined with case-oriented teaching in prosthodontics practice teaching.Methods:Thirty undergraduates majoring in stomatology who practiced in West China/Hospital of Stomatology, Sichuan University from July 2019 to July 2020 were selected and randomly divided into the control group ( n=15) and the observation group ( n=15). The control group used the case-oriented teaching, and the observation group adopted the GLTC doctor-patient communication mode combined with the case-oriented teaching. The performance of clinical practice (theoretical performance, medical record analysis performance, operation performance, lecture performance), interns' doctor-patient communication ability, and interns' teaching subjective evaluation of the two groups were evaluated. SPSS 22.0 was used for t-test and Chi-square test. Results:The theoretical performance, medical record analysis performance, operation performance, and lecture performance of the interns in the observation group were better than those in the control group ( P < 0.05). The score of Liverpool doctor communication ability evaluation scale in the observation group was higher than that in the control group ( P<0.05). In the subjective evaluation of the teaching mode of the interns, the improvement of learning interest, collective awareness, language expression ability, comprehensive analysis ability, and clinical operation ability in the observation group were better than those in the control group ( P<0.05). Conclusion:The GLTC doctor-patient communication model combined with the case-oriented teaching method is effective in prosthodontics practice teaching, which can effectively improve the professional knowledge and skills of interns and improve their teaching satisfaction and doctor-patient communication ability.

Objective To investigate the effect of tantalum particles on the proliferation of osteoblasts and explore its mechanism. Methods Mouse osteoblasts MC3T3-E1 were co-cultured with micro-tantalum particles (micro-Ta)and Nano-tantalum particles(nano-Ta)of different concentrations respectively. CCK-8 assay was used to measure the cell viability at 6,12,24 and 48 h. According to the result of CCK-8 the group with the most prolif-erative effect was screened and the level of autophagy was detected by using Western blot,laser confocal microsco-py and transmission electron microscopy(SEM). Finally,to verify the role of autophagy in pro-proliferation effect of nano-Ta,the OD value was measured repeatedly in combination with autophagy inducer and inhibitor. Results 100 ng/mL micro-Ta treated groups had obvious proliferative effect but autophagy was not detected. 20 μg/mL nano-Ta treated groups had obvious proliferative effect and autophagy was detected. CCK-8 assay revealed that autophagy inhibitor can significantly inhibited cell proliferation of nano-Ta treated group. Conclusion Nano-Ta could pro-mote cell proliferation by inducing autophagy,and micro-Ta may promote osteoblast proliferation through other non-autophagy pathway.