Objective To investigate the feasibility of constructing a preeclampsia(PE)risk model based on multiple exosomal micrornas(miRNA)expression levels and to verify its efficacy in predicting PE.Methods A total of 1037 pregnant women who were archived in our hospital from June 2019 to December 2021 and whose gestational weeks were less than or equal to 20 weeks were selected as the research subjects.The expression of exosomal miRNA(including miR-155-5p,miR-215-5p,miR-203a-3p,miR-199a-5p and miR-125a-3p)in all samples was detected by qRT-PCR.Then,all patients were followed up to the end of pregnancy.The occurrence of PE during the follow-up period was counted,and all samples were divided into the PE group and the control group according to results.Cox regression was used to analyze the influencing factors of PE.The multi-miRNA risk model was constructed with ggrisk package,and the predictive effect of the model on PE was evaluated by receiver operating characteristic(ROC)curve.Results By the end of follow-up on October 31,2022,974 cases were finally followed up,and the follow-up completion rate was 93.92%.Among all the 974 patients who completed the follow-up,65 patients developed PE,so they were finally divided into the PE group,and 909 cases were used as the control group.The age,pre-pregnancy BMI and waist circumference at 12 weeks of gestation were higher in the PE group than those in the control group(P＜0.05).The proportions of smoking history and drinking history were higher in the PE group than those of the control group(P＜0.05).The contents of triglyceride(TG),low density lipoprotein cholesterol(LDL-C),total cholesterol(TC),alanyl aminotransferase(ALT),aspartate aminotransferase(AST),platelet distribution width(PDW),mean platelet volume(MPV),miR-155-5p,miR-199a-5p and miR-215-5p were higher in the PE group than those in the control group,while contents of thyroid stimulating hormone(TSH),miR-125a-3p and miR-203a-3p were lower in the PE group than those in the control group(P＜0.05).The expression levels of miR-125a-3p,miR-155-5p,miR-199a-5p and miR-215-5p were independent predictors of PE(P＜0.05).The predictive risk model constructed from the above miRNAs had good predictive value in the occurrence of PE(AUC=0.998),with a sensitivity of 98.46%(63/65)and a specificity of 93.94%(854/909).Conclusion miR-125a-3p,miR-155-5p,miR-199a-5p,miR-203a-3p and miR-215-5p are significantly related to the occurrence of PE,and the PE prediction model constructed with the above five miRNAs has better effect.

Objective To explore the risk factors of antibiotic related diarrhea occurrence in elderly pa-tients with acute exacerbation of chronic obstructive pulmonary disease (COPD) treated by non-invasive vent-ilator.Methods The clinical data of 248 elderly patients with acute exacerbation of COPD treated in this hos-pital from June 2020 to June 2023 were analyzed retrospectively.The univariate and multivariate logistic re-gression were used to analyze the influencing factors for the patients with antibiotic related diarrhea occur-rence.Results Among 248 elderly patients with acute exacerbation of COPD,49 cases (19.76％) developed antibiotic related diarrhea.The results of univariate and multivariate logistic regression analysis showed that the age≥70 years old,non-invasive ventilator treatment time≥7 d,broad-spectrum antibiotics use,antibiotic treatment time≥7 d,hospitalization duration≥10 d and severe illness condition were the risk factors for anti-biotic related diarrhea occurrence in the elderly patients with acute exacerbation of COPD (P<0.05),while the daily disinfection of ventilator tubes and masks was a protective factor (P<0.05).Conclusion The inci-dence rate of antibiotic-related diarrhea in the elderly patients with acute exacerbation of COPD treated by non-invasive ventilator is high,which is closely related to the age of patients,ventilation time,ventilator pipe-line and mask disinfection,antibiotic use time,whether using broad-spectrum antibiotics,hospitalization dura-tion and severity of disease condition.

This paper reviews the clinical research on the treatment of chronic prostatitis with traditional Chinese medicine in recent years. It is believed that the core of the etiology and pathogenesis of this disease lies in the deficiency of spleen and kidney, dampness heat, phlegm turbid and blood stasis toxin. If the disease lasts for a long time, the spleen and kidney become to the deficiency from excess. The internal treatment of traditional Chinese medicine includes syndrome differentiation, disease differentiation or single prescription, while the external treatment mainly includes herbal bathing, enema, anal administration and acupuncture. Traditional Chinese medicine has advantages in the treatment of this disease. We should continue to study the the mechanism of traditional Chinese medicine for this disease, standardize the syndrome differentiation and treatment and outocmes evaluation, and carry out research on famous prescriptions on prostatitis.