Metastasis serves as an indicator of malignancy and is a biological characteristic of carcinomas. Epithelial-mesenchymal transition (EMT) plays a key role in the promotion of tumor invasion and metastasis and in the enhancement of tumor cell aggressiveness. Prostaglandin E synthase 3 (p23) is a cochaperone for heat shock protein 90 (HSP90). Our previous study showed that p23 is an HSP90-independent transcription factor in cancer-associated inflammation. The effect and mechanism of action of p23 on lung cancer metastasis are tested in this study. By utilizing cell models in vitro and mouse tail vein metastasis models in vivo, the results provide solid evidence that p23 is critical for promoting lung cancer metastases by regulating downstream CXCL1 expression. Rather than acting independently, p23 forms a complex with RNA-binding motif protein 14 (RBM14) to facilitate EMT progression in lung cancer. Therefore, our study provides evidence for the potential role of the RBM14-p23-CXCL1-EMT axis in the metastasis of lung cancer.

Objective By observing the effects of"three methods and three points"tuina technique on the expression of interleukin-17F(IL-17F),interleukin-17 receptor C(IL-17RC),activator 1 of nuclear transcription factor-κB(Act1),tumour necrosis factor receptor-associated factor 6(TRAF6)and M1 microglial cell expression in the spinal dorsal horn of rats with mild chronic compressive injury(minor CCI)model,we explored the immediate analgesic mechanism of tuina on peripheral neuropathic pain(pNP).Methods Thirty-six SD rats were divided into the sham group,the model group and the tuina group according to the random number method,twelve rats in each group,and the minor CCI model was replicated by ligating the right sciatic nerve.The rats in the tuina group were subjected to pointing,plucking and kneading at the BL37,BL57 and GB34 points on the affected side using a tuina simulator,while the sham group and the model group were only grasped and restrained,and were intervened for one time.The mechanical pain test and cold plate test were used to evaluate the response of rats to mechanical stimulation and cold stimulation after immediate intervention.The protein expression of IL-17F and TRAF6 in the spinal dorsal horn of rats in each group was detected by Western blotting.The mRNA expression of IL-17F,IL-17RC,Act1 and TRAF6 in the spinal dorsal horn of rats in each group was detected by real-time PCR.The average fluorescence intensity of M1 microglia in the spinal dorsal horn of rats in each group was detected by immunofluorescence.Results Behavioral results showed that before intervention,compared with the sham group,paw mechanical withdraw threshold(PMWT)decreased and cold sensitivity threshold(CST)increased in the model group and the tuina group;after tuina intervention,PMWT in the tuina group was increased,and CST was decreased compared with the model group;after intervention,PMWT in the tuina group was increased,while CST was decreased(P＜0.05).RT-PCR results showed that compared with the sham group,mRNA expression levels of IL-17F,IL-17RC,TRAF6 and Act1 in the spinal dorsal horn of the model group were increased;compared with model group,the mRNA expression levels of above indexes in the tuina group were decreased(P＜0.05).Western boltting results showed that compared with the sham group,the expression levels of IL-17F and TRAF6 protein in the spinal dorsal horn of the model group were increased;compared with the model group,the expression levels of IL-17F and TRAF6 protein in the tuina group decreased(P＜O.05).Immunofluorescence results showed that the mean fluorescence intensity of CD40 in the spinal dorsal horn of model group was enhanced compared with the sham group;compared with the model group,the mean fluorescence intensity of CD40 in the tuina group was decreased(P＜0.05).Conclusion The"three methods and three points"tuina technique can produce immediate analgesia by inhibiting the expression of IL-17F,IL-17RC,Act1,TRAF6 and the activation of M1 microglia in the dorsal horn of the spinal cord after one intervention.

Objective:To analyze the drug-resistant gene loci of Mycoplasma pneumoniae (MP) using metagenomic next-generation sequencing (mNGS). Methods:From November 2022 to October 2023, 697 clinical samples (including sputum, alveolar lavage fluid and blood) of 686 children with Mycoplasma pneumoniae positive detected by mNGS were retrospectively analyzed. Samples were divided into intensive care unit (ICU) group and non-ICU group, Chi-square test was used to compare groups, and Mann-Kendall trend test was used to analyze the change trend of the detection rate of drug resistance gene loci over time. Results:Of the 697 samples, 164 were from the ICU group and 533 were from the non-ICU group. The detection rate of Mycoplasma pneumoniae resistance gene was 44.3% (309/697), and all detected drug-resistant gene loci of MP were A2063G. The detection rate of Mycoplasma pneumoniae in ICU group was 50.0% (82/164), and the detection rates of Mycoplasma pneumoniae resistance gene loci in sputum, alveolus lavage fluid and blood samples were 75.0% (18/24) and 48.4% (62/128), respectively. The detection rate in sputum was higher than alveolus lavage fluid samples ( χ2=5.72, P=0.017). The detection rate of Mycoplasma pneumoniae in non-ICU group was 42.6% (227/533), the detection rate of Mycoplasma pneumoniae resistance gene loci in sputum and alveolar lavage fluid was 40.0% (16/40), 44.3% (201/454), and no detection rate in blood samples (0/12). There was no significant difference in the detection rate of alveolar lavage fluid and sputum ( χ2=0.27, P=0.602). From November 2022 to October 2023, the detection rate of submitted samples showed an increasing trend month by month (overall: Z=3.99, ICU inspection group: Z=2.93, non-ICU group: Z=3.01, all P<0.01). Among the bacteria commonly detected with Mycoplasma pneumoniae, Streptococcus pneumoniae accounted for the highest proportion, the detection rate was 15.5% (108/697), and Epstein-Barr virus accounted for the highest proportion of 17.6% (123/697). Conclusions:From November 2022 to October 2023, the detection rate of Mycoplasma pneumoniae drug resistance gene loci showed an increasing trend. The detection rate of drug resistance gene loci in sputum samples of ICU group was higher than alveolus lavage fluid. No new drug resistance site were detected.

Purpose: The purpose of this study is to compare and analyze the differences in biomechanical function and energy absorption of the lower extremity in the sagittal plane when single leg drop landing between groups with flat foot and normal foot. Methods: Twenty-eight healthy men in their 20s were classified into 13 with flat foot and 15 with normal foot through evaluation of navicular drop test. Using a motion analysis system, loading rate (N/sec), peak vertical ground reaction force (N/body weight [BW]), sagittal plane hip, knee, and ankle joint range of motion (°), peak moment (Nm/BW), peak joint power (W/BW) and peak joint work (J/BW) were calculated and analyzed during single leg drop landing. Results: During single leg drop landing, the flat foot and normal foot groups showed no significant differences in loading rate, peak vertical ground reaction force, hip and knee joint range of motion, peak knee and ankle joint moment, peak joint power, and peak joint work (p＞ 0.05). However, the flat foot group showed greater ankle range of motion and peak hip joint flexion moment compared to the normal foot group (p=0.040 and p=0.018, respectively). Conclusion The flat foot group shows sagittal plane landing mechanics that are different from the normal foot group during single leg drop landing and appears to try to distribute shock by relying on the distal joint compared to the normal foot group.

Purpose: The purpose of this study is to compare and analyze the differences in biomechanical function and energy absorption of the lower extremity in the sagittal plane when single leg drop landing between groups with flat foot and normal foot. Methods: Twenty-eight healthy men in their 20s were classified into 13 with flat foot and 15 with normal foot through evaluation of navicular drop test. Using a motion analysis system, loading rate (N/sec), peak vertical ground reaction force (N/body weight [BW]), sagittal plane hip, knee, and ankle joint range of motion (°), peak moment (Nm/BW), peak joint power (W/BW) and peak joint work (J/BW) were calculated and analyzed during single leg drop landing. Results: During single leg drop landing, the flat foot and normal foot groups showed no significant differences in loading rate, peak vertical ground reaction force, hip and knee joint range of motion, peak knee and ankle joint moment, peak joint power, and peak joint work (p＞ 0.05). However, the flat foot group showed greater ankle range of motion and peak hip joint flexion moment compared to the normal foot group (p=0.040 and p=0.018, respectively). Conclusion The flat foot group shows sagittal plane landing mechanics that are different from the normal foot group during single leg drop landing and appears to try to distribute shock by relying on the distal joint compared to the normal foot group.

Purpose: The purpose of this study is to compare and analyze the differences in biomechanical function and energy absorption of the lower extremity in the sagittal plane when single leg drop landing between groups with flat foot and normal foot. Methods: Twenty-eight healthy men in their 20s were classified into 13 with flat foot and 15 with normal foot through evaluation of navicular drop test. Using a motion analysis system, loading rate (N/sec), peak vertical ground reaction force (N/body weight [BW]), sagittal plane hip, knee, and ankle joint range of motion (°), peak moment (Nm/BW), peak joint power (W/BW) and peak joint work (J/BW) were calculated and analyzed during single leg drop landing. Results: During single leg drop landing, the flat foot and normal foot groups showed no significant differences in loading rate, peak vertical ground reaction force, hip and knee joint range of motion, peak knee and ankle joint moment, peak joint power, and peak joint work (p＞ 0.05). However, the flat foot group showed greater ankle range of motion and peak hip joint flexion moment compared to the normal foot group (p=0.040 and p=0.018, respectively). Conclusion The flat foot group shows sagittal plane landing mechanics that are different from the normal foot group during single leg drop landing and appears to try to distribute shock by relying on the distal joint compared to the normal foot group.

Purpose: The purpose of this study is to compare and analyze the differences in biomechanical function and energy absorption of the lower extremity in the sagittal plane when single leg drop landing between groups with flat foot and normal foot. Methods: Twenty-eight healthy men in their 20s were classified into 13 with flat foot and 15 with normal foot through evaluation of navicular drop test. Using a motion analysis system, loading rate (N/sec), peak vertical ground reaction force (N/body weight [BW]), sagittal plane hip, knee, and ankle joint range of motion (°), peak moment (Nm/BW), peak joint power (W/BW) and peak joint work (J/BW) were calculated and analyzed during single leg drop landing. Results: During single leg drop landing, the flat foot and normal foot groups showed no significant differences in loading rate, peak vertical ground reaction force, hip and knee joint range of motion, peak knee and ankle joint moment, peak joint power, and peak joint work (p＞ 0.05). However, the flat foot group showed greater ankle range of motion and peak hip joint flexion moment compared to the normal foot group (p=0.040 and p=0.018, respectively). Conclusion The flat foot group shows sagittal plane landing mechanics that are different from the normal foot group during single leg drop landing and appears to try to distribute shock by relying on the distal joint compared to the normal foot group.

Purpose: The purpose of this study is to compare and analyze the differences in biomechanical function and energy absorption of the lower extremity in the sagittal plane when single leg drop landing between groups with flat foot and normal foot. Methods: Twenty-eight healthy men in their 20s were classified into 13 with flat foot and 15 with normal foot through evaluation of navicular drop test. Using a motion analysis system, loading rate (N/sec), peak vertical ground reaction force (N/body weight [BW]), sagittal plane hip, knee, and ankle joint range of motion (°), peak moment (Nm/BW), peak joint power (W/BW) and peak joint work (J/BW) were calculated and analyzed during single leg drop landing. Results: During single leg drop landing, the flat foot and normal foot groups showed no significant differences in loading rate, peak vertical ground reaction force, hip and knee joint range of motion, peak knee and ankle joint moment, peak joint power, and peak joint work (p＞ 0.05). However, the flat foot group showed greater ankle range of motion and peak hip joint flexion moment compared to the normal foot group (p=0.040 and p=0.018, respectively). Conclusion The flat foot group shows sagittal plane landing mechanics that are different from the normal foot group during single leg drop landing and appears to try to distribute shock by relying on the distal joint compared to the normal foot group.

OBJECTIVE: The study explores the effects of electroacupuncture (EA) at the governing vessel (GV) on proteomic changes in the hippocampus of rats with cognitive impairment. METHODS: Healthy male rats were randomly divided into 3 groups: sham, model and EA. Cognitive impairment was induced by left middle cerebral artery occlusion in the model and EA groups. Rats in the EA group were treated with EA at Shenting (GV24) and Baihui (GV20) for 7 d. Neurological deficit was scored using the Longa scale, the learning and memory ability was detected using the Morris water maze (MWM) test, and the proteomic profiling in the hippocampus was analyzed using protein-labeling technology based on the isobaric tag for relative and absolute quantitation (iTRAQ). The Western blot (WB) analysis was used to detect the proteins and validate the results of iTRAQ. RESULTS: Compared with the model group, the neurological deficit score was significantly reduced, and the escape latency in the MWM test was significantly shortened, while the number of platform crossings increased in the EA group. A total of 2872 proteins were identified by iTRAQ. Differentially expressed proteins (DEPs) were identified between different groups: 92 proteins were upregulated and 103 were downregulated in the model group compared with the sham group, while 142 proteins were upregulated and 126 were downregulated in the EA group compared with the model group. Most of the DEPs were involved in oxidative phosphorylation, glycolipid metabolism and synaptic transmission. Furthermore, we also verified 4 DEPs using WB technology. Although the WB results were not exactly the same as the iTRAQ results, the expression trends of the DEPs were consistent. The upregulation of heat-shock protein β1 (Hspb1) was the highest in the EA group compared to the model group. CONCLUSION EA can effect proteomic changes in the hippocampus of rats with cognitive impairment. Hspb1 may be involved in the molecular mechanism by which acupuncture improves cognitive impairment.
Rats ; Male ; Animals ; Rats, Sprague-Dawley ; Electroacupuncture ; Proteomics ; Cognitive Dysfunction/therapy* ; Hippocampus

Objective:To investigate the therapeutic effects of different surgical methods on papillary thyroid carcinoma of the isthmus (PTCI) and their effects on parathyroid function and thyroglobulin.Methods:Eighty patients with PTCI who underwent treatment in Zhejiang Xin'an International Hospital from January 2016 to January 2021 were included in this study. They were randomly allocated to undergo ipsilateral lobectomy with removal of the isthmus combined with ipsilateral central neck lymph node dissection (group A, n = 40) or total thyroidectomy combined with ipsilateral central neck lymph node dissection (group B, n = 40). We compared intraoperative and postoperative conditions, complications, and hypoparathyroidism between the two groups. We also compared serum thyroglobulin level measured before and 3 days after surgery between the two groups. Results:Operative time was significantly shorter in group A than in group B [(78.95 ± 13.52) minutes vs. (104.23 ± 27.38) minutes, t = -5.23, P < 0.05]. Intraoperative blood loss was significantly less in group A than in group B [(52.32 ± 6.59) mL vs. (75.41 ± 9.98) mL, t = -12.21, P < 0.05]. There were no significant differences in voice handicap index and reflux symptom index scores between the two groups (both P > 0.05). The incidence of complications was significantly lower in group A than in group B (10.00% vs. 30.00%, χ2 = 5.00, P < 0.05). The incidence of hypoparathyroidism was significantly lower in group A than in group B (7.50% vs. 27.50%, χ2 = 5.54, P < 0.05). At 3 days after surgery, serum thyroglobulin level was significantly lower in group A than in group B [(0.82 ± 0.17) μg/L vs. (1.26 ± 0.23) μg/L, t = -9.73, P < 0.05]. Conclusion:Ipsilateral lobectomy with removal of the isthmus combined with ipsilateral central neck lymph node dissection is more effective on PTCI than total thyroidectomy combined with ipsilateral central neck lymph node dissection. The former has little effect on parathyroid function and can reduce serum thyroglobulin level. The study is highly innovative and scientific.