We studied the genetic diversity and established the DNA molecular identify for Prunus mume with both ornamental and edible values, aiming to collect, identify, evaluate, and breed new varities of this plant and promote the upgrading of the P. mume industry chain in northern China. We employed 13 pairs of primers with good polymorphism, clear bands, and good repeatability to analyze the genetic diversity and establish the molecular identify of 68 germplasm accessions of P. mume with both ornamental and edible values from Xingtai, Hebei Province. We then employed the unweighted pair-group method with arithmetic means (UPGMA) to perform the cluster analysis based on genetic distance. After that, we analyzed the genetic structure of the 68 germplasm accessions based on a Bayesian model. The 13 pairs of SSR primers amplified a total of 124 alleles from 68 P. mume germplasm accessions, with the mean number of alleles (Na) of 9.538 5, the minor allele frequency (MAF) of 0.369 3, the mean number of effective alleles (Ne) of 4.483 5, and the mean Shannon genetic diversity index (I) of 1.712 4. The mean Nei's gene diversity index (H) of 0.763 7, the mean observed heterozygosity (Ho) of 0.719 5, the mean expected heterozygosity (He) of 0.769 3, the mean polymorphism information content (PIC) of 0.733 6, and the mean genetic similarity (GS) of 0.772 9 suggested that there were significant genetic differences and rich genetic diversity among the studied P. mume germplasm accessions. The cluster analysis revealed that the 68 accessions were classified into three groups, with the mean genetic distance of 0.622 6. The population structure analysis classified the germplasm accessions into two populations. According to the PIC of primers, we selected primers for combination and constructed the combination with the fewest primers required for germplasm differentiation of P. mume with both ornamental and edible values. This study provides a theoretical basis for the innovation and industrial upgrading of P. mume with both ornamental and edible values in gardening and the improvement of breeding efficiency.
Prunus/classification* ; Microsatellite Repeats/genetics* ; Genetic Variation ; China ; Phylogeny ; Polymorphism, Genetic ; DNA, Plant/genetics* ; Alleles

BACKGROUND: We classified the hips of Korean women into 6 types based on shape and contour, and have performed gluteal augmentation using Elastomere implants with lipoplasty of the flank, gluteal fold, and saddle bags of the thigh according to the hip type. METHODS: We analyzed the shapes and contour of the buttocks of 148 patients from September 2009 to September 2015. We performed buttock augmentation with implants using Gonzalez's XYZ method, as well as liposuction and fat injection lipoplasty of the surrounding areas in these 148 patients. RESULTS: We defined the following 6 types: the oval type (30%), the straight type (17%), the peach type (9%), the trapezoid type (20%), the heart type (11%), and the W type (13%). Ninety-seven patients underwent hip augmentation with an implant only or combined with lipoplasty, and 51 patients underwent lipoplasty for buttock shape correction. Patients were followed up for at least 3 months, with the longest follow-up being 4 years. Implants were removed postoperatively in 8 of 97 cases. CONCLUSIONS: The hip augmentation not only involves the simple insertion of implants, but also complex surgical procedures on the surrounding flank and thigh areas. Appropriate procedures should be utilized based on the 6 different hip types to ensure the best outcomes.
Asian Continental Ancestry Group ; Buttocks ; Classification ; Elastomers ; Female ; Follow-Up Studies ; Heart ; Hip ; Humans ; Lipectomy ; Methods ; Prunus persica ; Reconstructive Surgical Procedures ; Surgical Flaps ; Thigh

Mei (Prunus mume Sieb.et Zucc.) is traditionally not only a famous special ornamental plant but also a fruit tree origined in China. In order to conserve and ultilize scientifically the germplasm resources of wild mei, we identified and analysed the germplasm of mei flower in 65 samples collected from the habitat, using AFLP makers in combination with morphological anaylsis. This study amplified clearly 1 728 polymorphic bands, using the 8- pair-primer of Mse I -EcoR I screened totally from 64 -primer combination. According to the Nei' 72 distance coefficient clustering, all of the formas and varieties used in this study, including Prunus mume var. mume, P. mume, P. mume var. goethartiana, P. mume var. pallescens, P. mume var. microcarpa, P. mume var. cenrnus-sempervirens (newly recoeding variety), P. mume var. cernua, P. mume var. pallidus, P. mume var. taomei, were identified at the point of Nei' 72=0.26. Due to the genetic difference obviously among the formas and varieties, we suggested that wild germplasmtypes of all formas and varieties in P. mume should be conserved in their habitats in the furture.
Amplified Fragment Length Polymorphism Analysis ; Genetic Variation ; Germ Cells, Plant ; classification ; Phylogeny ; Prunus ; classification ; genetics ; Species Specificity