1.Correlation between growth characteristics and quality of rhizomes of Notopterygium incisum under wild tending.
Ping YANG ; Hong-Lan WANG ; Hui SUN ; Wen-Tao ZHU ; Tao QIU ; Jiu-Zhen DU ; Hong-Bing SUN ; Yi ZHOU ; Shun-Yuan JIANG
China Journal of Chinese Materia Medica 2020;45(4):739-745
Study the growth and development process of rhizomes(bamboo-like part) of Notopterygium incisum and the changes of carbohydrate, endogenous hormones and secondary metabolites, and provide theoretical guidance for the formation of high-quality N. incisum medicinal commodities under artificial cultivation. The One-year-old seedlings were transplanted to the original habitat,and the growth and physiological characteristics of N. incisum were dynamically monitored. The results showed that: ① Seedlings transplanted to the original habitat in spring could form rhizomes(bamboo-like part) in the same year. ② After 60 days of transplantation, the root length and root diameter of underground part of N. incisum had increased rapidly, and carbohydrate content in roots and rhizomes had accumulated rapidly. After 120 days of transplantation, the roots and rhizomes of underground part had grown slowly, and starch content in roots and rhizomes increased continuously, while sucrose and total soluble sugar content decreased gradually. ③ The content of abscisic acid(ABA) in rhizomes decreased firstly and then increased, while the indole acetic acid(IAA) content stabilized firstly and then increased rapidly, and the contents of gibberellin(GA_3) and zeatin riboside(ZR) continued to increase. ④ The content of notopterol in rhizomes was higher than that in roots, while the content of isoimperatorin was lower than that in roots, but the total content of the both in rhizomes was higher than that in roots. Therefore, N. incisum can form rhizomes with high content of secondary metabolites under wild tending, and the growth and development of rhizomes are closely related to changes in carbohydrates and are regulated by related endogenous hormones.
Apiaceae/growth & development*
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Ecosystem
;
Phytochemicals/analysis*
;
Plant Roots/growth & development*
;
Plants, Medicinal/growth & development*
;
Rhizome/growth & development*
;
Secondary Metabolism
;
Seedlings/growth & development*
2.Ecological benefits of Gastrodia elata-Phallus impudicus sequential planting pattern.
Jin-Qiang ZHANG ; Tao ZHOU ; Lan-Ping GUO ; Wei-Ke JIANG ; Xiao WANG ; Guang-Wen ZHANG ; Cheng PAN ; Peng LIU ; Yong-Zhi CAO ; Cheng-Hong XIAO
China Journal of Chinese Materia Medica 2020;45(3):457-462
Gastrodia elata is a kind of precious traditional Chinese medicine. In the process of cultivation of G. elata, due to the influence of continuous cropping obstacles and other factors, the fungus materials and land that have been planted with G. elata are often abandoned, resulting in a great waste of resources. Based on the planting characteristics of G. elata and Phallus impudicus and the previous research experience in ecological agriculture, this paper analyzed the ecological adaptability characteristics of G. elata and P. impudicus, and summarized the key techniques of the G. elata-P. impudicus sequential planting pattern. Keeping track of the planting area, fungus-growing materials consumption and market sales of G. elata-P. impudicus sequential planting pattern, the ecological benefits of G. elata-P. impudicus sequential planting pattern from the aspects of utilization rate of fungus-growing materials were analyzed, the value of land resources per unit area, ecological environmental protection, labor cost and economic benefits were consi-dered. The technical principle of G. elata-P. impudicus sequential planting pattern was expounded according to their ecological habit, the season of harvest and planting, the difference of composition of fungus-growing materials, and the microbial ecology. The sequential planting pattern of G. elata-P. impudicus not only realized the double production of medicinal materials and edible fungi, reduced the waste of old fungus-growing materials, but also transformed the energy from nutrition-supplied fungi to edible and medicinal fungi, which guaranteed the ecological recycling and utilization of G. elata in the process of cultivation.
Agaricales/growth & development*
;
Agriculture/methods*
;
Gastrodia/growth & development*
;
Medicine, Chinese Traditional
;
Plants, Medicinal/growth & development*
3.Transcriptomic analysis in Anemone flaccida rhizomes reveals ancillary pathway for triterpene saponins biosynthesis and differential responsiveness to phytohormones.
Guo-Yan MO ; Fang HUANG ; Yin FANG ; Lin-Tao HAN ; Kayla K PENNERMAN ; Li-Jing BU ; Xiao-Wei DU ; Joan W BENNETT ; Guo-Hua YIN
Chinese Journal of Natural Medicines (English Ed.) 2019;17(2):131-144
Anemone flaccida Fr. Schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents. In China, the rhizomes are used as treatments for a variety of ailments including arthritis. However, yields of the saponins are low, and little is known about the plant's genetic background or phytohormonal responsiveness. Using one-quarter of the 454 pyrosequencing information from the Roche GS FLX Titanium platform, we performed a transcriptomic analysis to identify 157 genes putatively encoding 26 enzymes involved in the synthesis of the bioactive compounds. It was revealed that there are two biosynthetic pathways of triterpene saponins in A. flaccida. One pathway depends on β-amyrin synthase and is similar to that found in other plants. The second, subsidiary ("backburner") pathway is catalyzed by camelliol C synthase and yields β-amyrin as minor byproduct. Both pathways used cytochrome P450-dependent monooxygenases (CYPs) and family 1 uridine diphosphate glycosyltransferases (UGTs) to modify the triterpenoid backbone. The expression of CYPs and UGTs were quite different in roots treated with the phytohormones methyl jasmonate, salicylic acid and indole-3-acetic acid. This study provides the first large-scale transcriptional dataset for the biosynthetic pathways of triterpene saponins and their phytohormonal responsiveness in the genus Anemone.
Anemone
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drug effects
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genetics
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metabolism
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Biosynthetic Pathways
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drug effects
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genetics
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Cytochrome P-450 Enzyme System
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genetics
;
metabolism
;
Gene Expression Profiling
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Gene Expression Regulation, Plant
;
drug effects
;
Glycosyltransferases
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genetics
;
metabolism
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Oleanolic Acid
;
analogs & derivatives
;
metabolism
;
Plant Growth Regulators
;
pharmacology
;
Plant Proteins
;
genetics
;
metabolism
;
Plants, Medicinal
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Rhizome
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drug effects
;
genetics
;
metabolism
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Saponins
;
metabolism
;
Triterpenes
;
metabolism
4.Research on protection mode of Dao-di herbs-taking the protection mode of geographical indications as an example.
An-Qi JIN ; Xiu-Lian CHI ; Guang YANG ; Feng GAO ; Meng CHENG ; Ling QUE ; Min CHEN
China Journal of Chinese Materia Medica 2019;44(3):619-623
Dao-di herbs have been synonymous with quality medicinal herbs in our mind hearts since ancient times and are the essence of Chinese medicine culture. The development of genuine regional herbs faces problems such as insufficient supply of local resources and lack of competitive advantages. It is necessary to seek a suitable model to protect and develop authentic medicinal materials. The protection mode of geographical indication products has become more mature. Geographical indication(GI) products and authentic medicinal materials are all related to the origin. There is certain commonality between them. However, there are also differences between authentic Chinese herbal medicines and geographical indications, and it is unsuitable to simply equate the authentic Chinese medicinal materials with GI products. This article comprehensively combs and compares the development of the authentic Chinese herbal medicines and geographical indication products, and believes that it is necessary to fully learn from the experience of GI products and combine the characteristics of authentic medicinal materials to establish a set of protection systems that meet the characteristics of authentic medicinal materials. Through this research, it has positive significance to promote the protection and management of authentic medicinal materials.
Drugs, Chinese Herbal
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Geography
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Medicine, Chinese Traditional
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Plants, Medicinal
;
growth & development
;
Research
5.Investigation and application of Dendrobium resources in Qinba Mountains with a discussion on industrial poverty alleviation model.
Xing-Jia MING ; Yan ZHANG ; Yong-de WANG ; Gang WANG ; Ji-Feng ZHAO ; Chang-Hua WANG ; Guang-Ping LIAO ; Xian-You QU
China Journal of Chinese Materia Medica 2019;44(3):614-618
The Dendrobium species are rare and endangered medicinal plants, and it is difficult to investigate their wild resources with conventional methods because of typical epiphytic herbaceous. We explored Dendrobium resources(include culture resource) of Qinba Mountains and the boundary Mountain area in Hubei, Chongqing using the methods of literatures and field investigation, and found that the cultural base of Dendrobium were profound in Qinba Mountains region. Furthermore, its germplasm resources of Dendrobium were established for the first time in Wanzhou Luotian town. In case the advantages of local rock resources and poverty alleviation demand, we have actively carried out the cultivating mode of Dendrobium which grow on rock, and the poverty alleviation model of local characteristic Dendrobium industry were established preliminarily. Our application case can provide reference for the mining and transformation of traditional Chinese medicine resources census results.
Agriculture
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economics
;
China
;
Dendrobium
;
growth & development
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Medicine, Chinese Traditional
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Plants, Medicinal
;
growth & development
;
Poverty
6.Research on regional distribution and industrial status analysis of genuine medicinal resources of flowers in Henan province.
Yong-Liang YU ; Lan-Jie XU ; Hong-Qi YANG ; Zheng-Wei TAN ; Wei DONG ; Wei XIA ; Yan-Ping FENG ; Feng LUO ; Hai-Ling LU ; Long WANG ; Qi-Cheng ZHANG ; Hui-Zhen LIANG
China Journal of Chinese Materia Medica 2019;44(5):899-904
Flower medicinal materials usually refer to Chinese medicinal materials with a complete flower,inflorescence,or part of a flower as the different medicinal parts,they have an important share in the Chinese herbal medicine market and appeared frequently in Chinese medicine prescriptions. Firstly,the species and regional distribution of the flower medicinal materials resources in China were briefly summarized. Secondly,the characteristics,yield,producing area and origin distribution of the main flower medicinal materials in Henan province were discussed. Finally,the present situation and the main problems of the flower medicinal materials industry in Henan province were comprehensively analyzed,and the corresponding industrial development countermeasures were put forward.This research was intended to provide decision-making demonstration and scientific basis for the rational exploitation and utilization of resources,breeding of new varieties,planting division,production layout and the healthy and sustainable development of the flower medicinal materials industry in Henan province.
China
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Conservation of Natural Resources
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Drugs, Chinese Herbal
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Flowers
;
chemistry
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Industry
;
Plants, Medicinal
;
growth & development
;
Research
7.Construction of database of growth environment of Dao-di herbs.
Meng LI ; Xu-Can HOU ; Xian-Bao ZHANG ; Yun WANG
China Journal of Chinese Materia Medica 2019;44(14):3010-3014
The Dao-di herbs are recognized as high-quality Chinese medicines with good medicinal properties. The factors of their growth environment affects the quality of Dao-di herbs. Choosing the right place to produce,rationally planting appropriate local medicinal materials,and improving the quality and yield of authentic medicinal materials are prerequisites for ensuring the steady development of Chinese medicine industry. In this paper,a knowledge model of the relationship between organic medicinal materials and growth environment factors was constructed by integrating the dataset of environmental factors of medicinal materials,and a knowledge base was constructed to provide a decision for the study of suitable medicinal materials under different environmental conditions. Based on this,it is expected to provide a platform for further research on the relationship between the medicinal properties of medicinal herbs and environmental factors.
Asteraceae
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growth & development
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Databases, Factual
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Drugs, Chinese Herbal
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Ecosystem
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Medicine, Chinese Traditional
;
Plants, Medicinal
;
growth & development
8.Investigation and analysis on difference of cultivation technique situation of Salvia miltiorrhiza.
Xiao-Yu CHEN ; Chao HE ; Bin-Bin YAN ; Wen-Bin LI ; Ye-Ye GENG ; Jun-Ling HOU ; Wen-Quan WANG
China Journal of Chinese Materia Medica 2019;44(7):1314-1320
Salvia miltiorrhiza is one of the commonly used bulk medicinal materials, which has significant effect on cardiovascular disease, and are heavy demanded in Asia, Europe, North America, Russia and Africa. Consequently, increasing the yield and quality of S. miltiorrhiza has become a major concern worldwide. With the current wild resources of S. miltiorrhiza gradually decreasing, cultivated products occupy most of the markets. However, the cultivation area is widely distributed and the cultivation techniques is different, which lead to the quality and yield of S. miltiorrhiza in consistent. This paper combined visiting survey with document analysis to carry out the cultivation situation of S. miltiorrhiza in main cultivation areas of Shandong, Henan, Sichuan, Shanxi and Hebei provinces. There exist big differences of the ecological environment, mode of cultivation, fertilization, pest control, harvesting processing among the producing areas. We should carry on the ecological suitability zoning analysis and suitable cultivation of each area study to form a pattern of high quality and high yield for the sustainable development of S. miltiorrhiza cultivation.
Agriculture
;
methods
;
Europe
;
Plants, Medicinal
;
growth & development
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Salvia miltiorrhiza
;
growth & development
9.Quality assessment of soil fertility of planting Chrysanthemum morifolium areas of Macheng city based on minimum data set.
Yin LIU ; Hong-Yuan YAN ; Xiao-Hong OU ; Lan-Ping GUO ; Da-Hui LIU
China Journal of Chinese Materia Medica 2019;44(24):5382-5389
A minimal data set( MDS) for soil fertility evaluation of Chrysanthemum plantation areas of Macheng city was established by principal component analysis( PCA) combined with Norm values of soil fertility indices and correlation coefficients among indices. A radar map was used to visually reflect the fertility level of individual indicators. Then,the comprehensive index model was used to calculate the soil fertility quality index( SFQI),and the values of SFQI was used to cluster,and the results showed that MDS was composed of five indicators: organic matter( OM),total phosphate( TP),available phosphorus( Av P),available magnesium( Av Mg) and available ferrum( Av Fe). Radar maps showed that the fertility of available phosphorus( Av P) and available copper( Av Cu) was mostly different in the two town,and the fertility of available ferrum( Av Fe) is smallest different. Except for the effective manganese( Av Mn) fertility level of Huangtugang town was higher than that of Futianhe town,the rest were lower than that of Futianhe town. Through analysis,the sensitivity of SFQI value calculated by taking the contribution rate of MDS index in the principal component of the whole data set( TDS) as the weight was the highest,MDS could better replace TDS. The value of SFQI-MDS ranged from 0. 353 to 0. 833,with an average value of 0. 604 and a coefficient of variation of 22%. The results of SFQI-MDS clustering showed that soil fertility could be divided into four categories: grade Ⅰ( 0. 727-0. 833) was superior,accounting for 25. 0%,grade Ⅱ( 0. 615-0. 681)was good,accounting for 29. 2%,mainly distributed in Futianhe Town,grade Ⅲ( 0. 494-0. 589) was medium,accounting for29. 1%,and grade Ⅳ( 0. 353-0. 419) was poor,accounting for 16. 7%,mainly distributed in Huangtugang town. Soil fertility of Futianhe town was better than that of Huangtugang town. It is suggested that boron fertilizer and potassium fertilizer should be supplemented to Chrysanthemum morifolium in production practice,and the amount of phosphate fertilizer,magnesium fertilizer and nitrogen fertilizer should be increased appropriately. At the same time,the amount of organic fertilizer should be increased to enhance soil fertility and improve soil physical and chemical properties.
China
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Chrysanthemum/growth & development*
;
Fertilizers
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Magnesium
;
Nitrogen
;
Phosphates
;
Phosphorus
;
Plants, Medicinal/growth & development*
;
Soil/chemistry*
10.Assessment of soil fertility for cultivation of Chinese herbal medicines.
Jing-Yi JIANG ; Wan-Zhen YANG ; Chuan-Zhi KANG ; Ya-Hui LIU ; Sheng WANG ; Kai SUN ; Lu-Qi HUANG ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2018;43(4):847-852
The soil fertility has great impacts on the yielding of Chinese medicinal materials, as well as the generation of major components. The practices showed that soil fertility has been decayed with the growth of cultivation years,which is leading to adverse effect on quality and quantity of Chinese medicinal materials. However, there was a lack of domestic unified standard for assessment of soil fertility of Chinese medicinal material cultivation, which has seriously limited the soil management and quality control. In this text, we reviewed the progress on research of soil fertility evaluation, built the soil fertility evaluation index including soil texture,soil bulk density,soil organic matter,soil acidity and alkalinity,soil available nitrogen,soil available phosphorus, soil available potassium,soil animals and soil microorganisms. It would provide the direction and thought for standard-settin on soil fertility evaluation of Chinese medicinal material cultivation.
Drugs, Chinese Herbal
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Nitrogen
;
chemistry
;
Phosphorus
;
chemistry
;
Plants, Medicinal
;
growth & development
;
Potassium
;
chemistry
;
Soil
;
chemistry

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