Objective:To investigate the effect and mechanism of hyperbaric oxygen combined with Wenshenyiqi granules on airway inflammation and oxidative stress in rats with chronic obstructive pulmonary disease (COPD).Methods:A total of 50 SD rats were randomly divided into control group (normal saline), model group (normal saline), Wenshenyiqi granules group (1.5 g/kg Wenshenyiqi granules), hyperbaric oxygen group (0.20 MPa hyperbaric oxygen) and combination group (0.20 MPa hyperbaric oxygen + 1.5 g/kg Wenshenyiqi granules) based on the order sorted by body mass, with 10 rats in each group. The COPD model was established in all the rats but the control group through smoking combined with airway infusion of lipopolysaccharide. After modeling, each group received treatment for 4 weeks according to the protocol. The pulmonary function, blood gas indicators, expressions of inflammatory factors in Bronchoalveolar Lavage Fluid (BALF), oxidative stress indicators in lung tissue, peroxisome proliferator activated receptor γ (PPARγ), nuclear factor-κB (NF-κB) mRNA, and protein expression were compared in each group.Results:After 4 weeks of treatment, the ratio of forced expiratory volume at 0.3 s to forced vital capacity (FEV 0.3/FVC) in the control group, the model group, the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group was (83.69±5.24), (62.30±6.07), (75.26±7.15), (75.87±6.02), and (81.85±6.33), respectively. The arterial partial pressure of carbon dioxide (PaCO 2) was (48.61±3.10), (57.92±4.23), (54.14±3.19), (54.08±3.62), and (49.32±3.87) mm Hg, respectively. Compared with the control group, the FEV 0.3/FVC was decreased and PaCO 2 increased in the model group ( P<0.05). Compared with the model group, the FEV 0.3/FVC was increased in the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group, while the PaCO 2 was decreased ( P<0.05). Compared with the Wenshenyiqi granules group and the hyperbaric oxygen group, the combination group had higher FEV 0.3/FVC and lower PaCO 2 ( P<0.05). Compared with the control group, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1) in BALF, and malondialdehyde (MDA), expression levels of NF-κB mRNA and protein in lung tissues were increased in the model group, while superoxide dismutase (SOD), expression levels of PPARγ mRNA and protein were decreased ( P<0.05). Compared with the model group, TNF-α, IL-1β, and MCP-1 in BALF, MDA and expression levels of NF-κB mRNA and protein in lung tissues were decreased in the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group, with those of the combination group were lower than those of the Wenshenyiqi granules group and the hyperbaric oxygen group ( P<0.05). Compared with the model group, the SOD and expression levels of PPARγ mRNA and protein in the lung tissues of the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group were all increased, with those of the combination group were higher than those of the Wenshenyiqi granules group and the hyperbaric oxygen group ( P<0.05). Conclusion:Hyperbaric oxygen combined with Wenshenyiqi granules can effectively inhibit airway inflammation and oxidative stress in rats with COPD, which may be achieved through PPARγ signaling pathway.

Objective:To investigate the effect and mechanism of hyperbaric oxygen combined with Wenshenyiqi granules on airway inflammation and oxidative stress in rats with chronic obstructive pulmonary disease (COPD).Methods:A total of 50 SD rats were randomly divided into control group (normal saline), model group (normal saline), Wenshenyiqi granules group (1.5 g/kg Wenshenyiqi granules), hyperbaric oxygen group (0.20 MPa hyperbaric oxygen) and combination group (0.20 MPa hyperbaric oxygen + 1.5 g/kg Wenshenyiqi granules) based on the order sorted by body mass, with 10 rats in each group. The COPD model was established in all the rats but the control group through smoking combined with airway infusion of lipopolysaccharide. After modeling, each group received treatment for 4 weeks according to the protocol. The pulmonary function, blood gas indicators, expressions of inflammatory factors in Bronchoalveolar Lavage Fluid (BALF), oxidative stress indicators in lung tissue, peroxisome proliferator activated receptor γ (PPARγ), nuclear factor-κB (NF-κB) mRNA, and protein expression were compared in each group.Results:After 4 weeks of treatment, the ratio of forced expiratory volume at 0.3 s to forced vital capacity (FEV 0.3/FVC) in the control group, the model group, the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group was (83.69±5.24), (62.30±6.07), (75.26±7.15), (75.87±6.02), and (81.85±6.33), respectively. The arterial partial pressure of carbon dioxide (PaCO 2) was (48.61±3.10), (57.92±4.23), (54.14±3.19), (54.08±3.62), and (49.32±3.87) mm Hg, respectively. Compared with the control group, the FEV 0.3/FVC was decreased and PaCO 2 increased in the model group ( P<0.05). Compared with the model group, the FEV 0.3/FVC was increased in the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group, while the PaCO 2 was decreased ( P<0.05). Compared with the Wenshenyiqi granules group and the hyperbaric oxygen group, the combination group had higher FEV 0.3/FVC and lower PaCO 2 ( P<0.05). Compared with the control group, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1) in BALF, and malondialdehyde (MDA), expression levels of NF-κB mRNA and protein in lung tissues were increased in the model group, while superoxide dismutase (SOD), expression levels of PPARγ mRNA and protein were decreased ( P<0.05). Compared with the model group, TNF-α, IL-1β, and MCP-1 in BALF, MDA and expression levels of NF-κB mRNA and protein in lung tissues were decreased in the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group, with those of the combination group were lower than those of the Wenshenyiqi granules group and the hyperbaric oxygen group ( P<0.05). Compared with the model group, the SOD and expression levels of PPARγ mRNA and protein in the lung tissues of the Wenshenyiqi granules group, the hyperbaric oxygen group, and the combination group were all increased, with those of the combination group were higher than those of the Wenshenyiqi granules group and the hyperbaric oxygen group ( P<0.05). Conclusion:Hyperbaric oxygen combined with Wenshenyiqi granules can effectively inhibit airway inflammation and oxidative stress in rats with COPD, which may be achieved through PPARγ signaling pathway.