OBJECTIVES: To investigate the efficacy of Ag₂Se nanoparticles for eliminating intracellular Porphyromonas gingivalis (P. gingivalis) in esophageal cancer and examine the effect of P. gingivalis clearance on progression of esophageal cancer. METHODS: Ag₂Se nanoparticles were synthesized via a chemical synthesis method. The effects of Ag₂Se nanoparticles on P. gingivalis viability and colony-forming ability were assessed using fluorescence staining and colony formation assays. In a mouse model bearing subcutaneous murine esophageal cancer cell allograft with P. gingivalis infection, the effect of treatment with Ag₂Se nanoparticles on the abundance of P. gingivalis in the tumor tissues was quantified using RNAscope in situ hybridization and quantitative polymerase chain reaction (qPCR), and the changes in tumor volume were monitored. The biosafety of Ag₂Se nanoparticles was assessed by examining liver and kidney functions and pathological changes in the major organs of the mice. RESULTS: Transmission electron microscopy revealed that the synthesized Ag₂Se nanoparticles were uniformly dispersed spherical particles with a diameter around 50 nm. In vitro experiments demonstrated that exposure to Ag₂Se nanoparticles significantly reduced the viability and clonal proliferation capacity of P. gingivalis in a dose-dependent manner. In the tumor-bearing mice, treatment with Ag₂Se nanoparticles significantly reduced the abundance of P. gingivalis in tumor tissues and suppressed tumor cell proliferation. No significant damages to the liver and kidney functions or the major organs were observed in Ag₂Se nanoparticle-treated mice, demonstrating good biocompatibility of Ag₂Se nanoparticles. CONCLUSIONS Ag₂Se nanoparticles exhibit significant bactericidal and inhibitory effects against P. gingivalis, and can effectively eliminate intracellular P. gingivalis to suppress the growth of esophageal cancer allograft in mice, suggesting the potential of Ag₂Se nanoparticles in the treatment of esophageal cancer.
Animals ; Porphyromonas gingivalis/drug effects* ; Mice ; Esophageal Neoplasms/pathology* ; Nanoparticles ; Metal Nanoparticles ; Bacteroidaceae Infections ; Cell Line, Tumor

Objective:To explore the organizational identity, sense of coherence, perceived social support, and work engagement of surgical nurses in a traditional Chinese medicine hospital and the relationships between them.Methods:From March to May 2022, 450 surgical nurses were selected by convenience sampling from Guangdong Provincial Hospital of Traditional Chinese Medicine for a survey with a general information questionnaire, the Organizational Identification Scale, the Sense of Coherence Scale, the Perceived Social Support Scale, and the Utrecht Work Engagement Scale-9. SPSS 24.0 was used to perform Pearson correlation analysis. AMOS 24.0 was used for structural equation modeling.Results:The nurses' scores of organizational identity, sense of coherence, perceived social support, and work engagement were (26.00±5.00), (53.58±7.50), (47.47±7.50), and (30.06±1.00), respectively. The correlation analysis revealed positive correlations between organizational identity, sense of coherence, perceived social support, and work engagement ( r=0.369-0.741, all P<0.001). Sense of coherence played a partial mediating role between perceived social support and work engagement, and the mediating effect was 0.07, accounting for 12.07% of the total effect. Organizational identity and sense of coherence produced a chain mediating effect between perceived social support and work engagement, in which the mediating effect was 0.11, accounting for 18.97% of the total effect. Conclusions:Organizational identity and sense of coherence produce a chain mediating effect between perceived social support and work engagement in the surgical nurses of the traditional Chinese medicine hospital.

Objective To employ the mesh meta analysis to compare the effectiveness and safety of short-term reaching-standard treatment in the patients with moderate to severe atopic dermatitis among the different biologics.Methods The randomized controlled trials (RCT) were retrieved from the databases of CNKI,Chinese Biomedical Literature Database,Wanfang,VIP,PubMed,Cochrane Library and Embase data-bases.The retrieval time was from the database establishment to August,2023.The related literatures on the biologics for treating moderate to severe atopic dermatitis were collected.The literatures were screened by the inclusion and exclusion standards,the literature quality was evaluated and the data were extracted.Then the mesh meta analysis was performed by using RevMan 5.3 and Stata 16.0 softwares.Results A total of 754 ar-ticles were retrieved,and 11 articles were finally included,involving in 14 RCT with a total sample number of 5528 cases.There were 4 intervention methods,including placebo and 3 kinds of drugs.The drugs were Dupi-lumab,Lebrikizumab and Tralokinumab.The results showed that for each of effectiveness indicator,different medication regimens had different performance,after comprehensively meeting the treatment criteria,Dupri-uliumab was superior to Lebrikizumab,and Lebrikizumab was superior to Tralokinumab.There was no statis-tical difference in adverse reactions between the three drugs and placebo (P>0.05).Conclusion Dupilumab is currently the most effective biologic agent for short-term target treatment,capable of reducing inflamma-tion and improving skin symptoms.Future studies should further evaluate its long-term efficacy and safety to guide clinical application.

Objective:To compare quantitatively the quality of the canal isthmus retropreparation among three kinds of ultrasonic ret-rotips.Methods:1 20 maxillary bicuspid teeth with two canals and an isthmus were randomly assigned to 3 groups(n=40).The canal isthmus retropreparation were made respectively using ultrasonic niti tip RE2,diamond tip Berutti and #25K file.Image processing and analysis system were used to measure the preoperative and postoperative parameters for further evaluation.Results:The cutting volume of ultrasonic niti tip RE2 group was significantly less than those of other two groups(P<0.05).Conclusion:Ultrasonic niti tip RE2 is feasible in the canal isthmus retropreparation.

Objective:To compare the quality of the canal isthmus retropreparation among three ultrasonic retrotips.Methods:120 maxillary bicuspid teeth with 2 canals and an isthmus were randomly assigned to 3 groups(n =40).The canal isthmus were retro-preparated by ultrasonic Ni-Ti tip RE2,diamond tip Berutti and #25K file respectively.The quality of cavity margin,the number and type of the micro-cracks were evaluated by SEM.Data were statistically analysed by Kruskal Wallis Test of SPSS 13.0.Results:The cavity margin quality of ultrasonic Ni-Ti tip RE2 group was superior to that of other 2 groups(P =0.003);The number of micro-crack in Ni-Ti tip RE2 group was less than that of other 2 groups(P =0.003).The type of micro-cracks has no significant difference among the 3 groups(P =0.830).Conclusion:Ultrasonic Ni-Ti tip RE2 is feasible in the canal isthmus retropreparation.

Objective To summarize the key points in operative cooperation in laparoscopy and rigid choledochoscopy in the treatment of cholecystolithiasis complicated with calculus of bile duct.Methods 86 cases of cholecystolithiasis complicated with calculus of bile duct were treated with laparoscopy combined with rigid choledochoscopy.Perioperative nursing was conducted carefully.Results Operations on 84 of cases were successful and another 2 cases turned to open operations.The average bleeding volume was(20.3±1.3)mL and the average operation duration was(90.6±10.1)mins.The clearance rate of calculus was 91.7%. Conclusions The laparoscopy combined with rigid choledochoscopy is safe and minimal in invasion and high in clearance rate of calculus.The skillful operative cooperation is critical for the smooth performance of the operation.

Objective:To compare the quality of root-end preparation quantitatively between ultrasonic retrotips and traditional slow-speed handpiece. Methods: 27 maxillary second bicuspid teeth with two canals and an isthmus were randomly assigned to 3 groups.Root-end preparations were made respectively using ultrasonic diamond tip Berutti and niti tip RE2, ultrasonic 40~#K file and slow-speed handpiece with No.2 round bur. Image processing and analysis system were used to measure the preoperation and postoperation parameters for further evaluating the difference between the two techniques. Results: The cutting volume of ultrasonic instruments was less than slow-speed handpiece(P<0.000 1). Conclusion: Ultrasonic instrument is better in the root-end preparation than traditional slow-speed handpiece.

Objective:To compare the quality of root-end preparation quantitatively between ultrasonic retrotips and traditional slow-speed handpiece.Methods:27 maxillary second bicuspid teeth with two canals and an isthmus were randomly assigned to 3 groups.Root-end preparations were made respectively using ultrasonic diamond tip Berutti and niti tip RE2,ultrasonic #40K file and slow-speed handpiece with No.2 round bur.Image processing and analysis system were used to measure the preoperation and postoperation parameters for further evaluating the difference between the two techniques.Results:The cutting volume of ultrasonic instruments was less than slow-speed handpiece(P