Objective:To investigate the potential role of CD147 expressed in the mouse epididymal epithelial cells on sperm maturation.Methods:Lcn5-Cre mice which were specifically expressed in the principal cells in the segments 4-5 of mouse epididymis were bred with CD147 flox/flox to obtain 24 of the CD147 conditional knockout mice (CD147 cKO-Lcn5) and its counterpart CD147 flox/flox control mice. Computer-aided sperm analysis (CASA), A23187-induced sperm acrosome reaction, and in vitro fertilization (IVF) were used to evaluate the effects of CD147 on sperm functions. Results:After CD147 knockout in the mice segment 4-5 of the caput epididymis, there were no statistically significant differences in sperm motility and acrosome reaction. Of note, the two-cell ratio of CD147 cKO-Lcn5 mice (74.03%±2.39%) was significantly reduced compared with that of CD147 flox/flox mice (90.59%±2.39%) in the experiment for IVF ( P=0.012). Conclusion:CD147 deleted in the 4-5 caput epididymis has limited regulatory effects on sperm motility and acrosome reaction processes, but significantly affected the ratio of two-cell after IVF.

Objective:To investigate the potential role of CD147 expressed in the mouse epididymal epithelial cells on sperm maturation.Methods:Lcn5-Cre mice which were specifically expressed in the principal cells in the segments 4-5 of mouse epididymis were bred with CD147 flox/flox to obtain 24 of the CD147 conditional knockout mice (CD147 cKO-Lcn5) and its counterpart CD147 flox/flox control mice. Computer-aided sperm analysis (CASA), A23187-induced sperm acrosome reaction, and in vitro fertilization (IVF) were used to evaluate the effects of CD147 on sperm functions. Results:After CD147 knockout in the mice segment 4-5 of the caput epididymis, there were no statistically significant differences in sperm motility and acrosome reaction. Of note, the two-cell ratio of CD147 cKO-Lcn5 mice (74.03%±2.39%) was significantly reduced compared with that of CD147 flox/flox mice (90.59%±2.39%) in the experiment for IVF ( P=0.012). Conclusion:CD147 deleted in the 4-5 caput epididymis has limited regulatory effects on sperm motility and acrosome reaction processes, but significantly affected the ratio of two-cell after IVF.

Objective This experiment aimed to study the influence of deep sea water (DSW) on wound healing of mice .Methods 24 Mice were randomly divided into two groups :group DSW(n=12) and group sterilize tap water(STW)(n=12) ,freely feeding for 14 days respectively ,and calculated the amount of food and water .On the 15th day ,1 cm × 1 cm size of wound was established on the back area of mice ,and continued to feed with DSW and STW respectively .Tracking the wound healing rate .Specimen was taken in the edge of wound tissue on postoperative 3 ,5 ,7 days ,then observed histopathological changes .Results Compared group DSW with group STW ,there was no significant difference in the total amount of food and water .5 days after the formation of wounds ,the wound healing rate of group DSW was significantly higher than group STW .Histological observation :compared with group STW ,vascular endothelial cells and new capillaries of the group DSW was increased ,and group DSW had less inflammatory cell and more fibroblast cells proliferation .Conclusion deep sea water can promote wound healing .

Objective: To investigate the effects of trentment with hydroxythy starch(130/0.4) on the serum albumin and immunologic function in postoperative patients with obstructive jaundice.Methods: 24 patients with obstructive jaundice were randomly divided into the control group(n=12) and hydroxythy starch(130/0.4) (HS) group(n=12). The serum ALB was detected 1d, 3d, 5d, 7d after the operation,and the immunologic index were detected 1d and 7d after the operation.Results: The ALB content of control group and HS group were not significantly different in the postoperative 1d and 7 d(P>0.05) and had significant differences in the postoperative 3 d, 5 d (P<0.05).All immunologic index had no significant differences in the postoperative 1d, 7d (P>0.05).Conclusion: The ALB content of patients with obstructive jaundice may decrease postoperatively. Treatment with hydroxylthy starch(130/0.4) can alleviate the drop of the ALB content. But it has no effects on immunologic function.

A glycomic method was used to screen the aberrantly ?1-6 fucosylated glycoproteins related to HCC metastasis and analyze the alteration of CK8 both in its expression level and its glycan parts associated with metastatic ability. Based on the approach, 2-DE coupled with lectin affinity blot, lectin affinity precipitation followed by MALDI-TOF-MS/MS, the lens culinaris agglutinin (LCA) affinity glycoprotein profiles from MHCC97-L and MHCC97-H cells, two higher metastatic HCC cell lines, were obtained, in which a differentially displayed protein spot was indicated when compared with Hep3B, in the region within 55～60 ku in molecular mass and 4～6 in isoelectric point. The identification result was CK8 by MALDI-TOF-MS/MS. To confirm the relation between increased core-fucosylation of CK8 and HCC metastasis, LCA affinity precipitation was used to extract the ?1-6 fucosylated glycoproteins, followed by Western blot. And it was found that CK8 was highly fucosylated in both MHCC97-L and MHCC97-H cells compared to Hep3B. Immunofluorescence analysis and Western blot were used to detect its intracellular localization and its protein expression levels, indicating that CK8 distributed in cytoplasm and increased protein expressions in MHCC97-L and MHCC97-H cell lines. And further lectin binding studies found that CK8 has a high affinity for Con A in both MHCC97-H and Hep3B cells, indicating that CK8 was a glycoprotein with high-mannose type N-glycans. But the amount of the lectin RCA-1 binding to CK8 was greater in MHCC97-H than Hep3B, suggesting that CK8 contained the increased terminal galactose residues ?-1, 4-linked to GlcNAc in MHCC97-H. All the results suggested that the increase of CK8 in its protein expression level, core-fucosylation and terminal gal ?1,4 GlcNAc disaccharides might be related to HCC metastatic ability.