OBJECTIVE Aimed to investigate the impact of comorbid asthma on chronic rhinosinusitis with nasal polyps(CRSwNP)and identify key proteins and signaling pathways.METHODS Proteomic methods were employed to analyze differentially expressed proteins(DEPs)in nasal lavage fluid(NLF)from control,CRSwNP,and CRSwNP with asthma groups.DIA quantitative analysis technology was used to assess the gradient changes of DEPs among the three groups to determine key proteins affected by comorbid asthma in CRSwNP.RESULTS Compared to the control group,1 377 and 1 006 DEPs were identified in the CRSwNP and CRSwNP with asthma groups,respectively.Peroxiredoxin-5(PRDX5),Ran-Binding Protein 1(RanBP1)(upregulated),and Keratin 9(KRT9)(downregulated)were identified as key proteins affecting CRSwNP with asthma.CONCLUSION Comorbid asthma may promote the occurrence and development of nasal polyps through specific key proteins and signaling pathways,providing new molecular insights into the interaction between CRSwNP and asthma.

Objective To investigate the correlation between chemoradiotherapy sensitivity and serum microRNA(miR)levels in the patients with intermediate and advanced cervical cancer.Methods Totally 112 patients with intermediate and advanced cervical cancer admitted to Nanchong Central Hospital from January 2020 to March 2022 were included in the study.All the patients were treated with synchronous radiochemotherapy.The sensitivity of the patients to the treatment regimen was analyzed,and the patients were divided into high sensitivity group(n=68)and low sensitivity group(n=44)in accordance with the sensitivity of the patients.The expression levels of serum miR-136,miR-664 and miR-206 in all patients were detected by real-time fluorescent quantitative PCR,and their expression levels were compared between the high sensitivity group and the low sensitivity group.The value of assessing the sensitivity to radiochemotherapy was evaluated by analyzing the expression levels of miR-136,miR-664 and miR-206 by receiver operating characteristic(ROC)curve.The relationship between radiochemotherapy sensitivity and clinical pathological features was analyzed,and influencing factors affecting sensitivity were explored in the patients with intermediate and advanced cervical cancer.Results The expression levels of miR-136 and miR-664 in the high sensitivity group were significantly higher than those in the low sensitivity group(P<0.05),and the expression level of miR-206 was significantly lower than that in the low sensitivity group(P<0.05).ROC curve results showed that serum miR-136,miR-664 and miR-206 all displayed a certain evaluation value for sensitivity of the patients,and the areas under the curve were 0.834,0.910 and 0.855,respectively.There was statistical significance in International Federation of Gynecology and Obstetrics(FIGO)staging when comparisons were made between the two groups(P<0.05).Multivariate Logistic regression analysis revealed that miR-136,miR-664,miR-206 and FIGO staging were all independent influencing factors of radiochemotherapy sensitivity in the patients with intermediate and advanced cervical cancer(P<0.05).Conclusion The expression levels of miR-136,miR-664 and miR-206 were correlated with the radiochemotherapy sensitivity of the patients with cervical cancer.The down-regulation of miR-136,miR-664 and the up-regulation of miR-206 might indicate radiochemotherapy resistance of cervical cancer.

The free membrane of Eudragit L100/S100 which is pH-sensitive, colon-specific was prepared by plane casting films. The film humidity, species and amount of plasticizers, the ratio of membrane material was investigated. The rate of membrane permeability and mechanical properties were used as indicators of orthogonal experiment, and its related properties were studied. The results show that the mechanical properties of the membrane and phragmoid capacity are the best when 30% TEC was used as plasticizer; the ratio of membrane material have little effect on the rate of membrane permeability and mechanical properties. By adjusting the species and amount of plasticizers, the ratio of Eudragit L100/S100, the free membrane which is colon-specific can be obtained.

OBJECTIVETo prepare Qixian decoction pellets.

METHODThe formulation and technological factors influencing the preparation of Qixian decoction were investigated in tangential spray fluid bed choosing the yield of pellets, particle diameter distribution, repose angle, bulk density as inspecting indexes.

RESULTthe technological parameters for the preparation of blank pellets were as follows: the ratio of starch and dextrin was 2:1, the adhesive agent was 70% syrup, the rotating speed was 200 r x min(-1), the air blow flow was 15 x 20 L x min(-1), the rate of air flow was 15 L x min(-1), the spay air pressure was 0.15 MPa, and the rotating rate of spray solution pump was 20-50 r x min(-1); The optimized technological parameters for the preparation of Qixian decoction were as follows: the relative density of the extract was 1.12-1.15 g x min(-1), the diluent was MCC and its quantity was 8%, the rotating rate of spray solution pump was 10-12 mL x min(-1), the frequency of the rotor disc was 18-20 Hz, the atomizing pressure was 0.2 MPa, the frequency of the fan was 22 Hz, and the spheronisation and drying time was 30 mins.

CONCLUSIONthe appearance of the Qixian decoction pellets prepared in tangential spray fluid bed are smoothing and round, the yield of pellets are high, and pellets of the particle size between 500-700 microm is 90.6%.

Adhesives ; chemistry ; Drug Compounding ; instrumentation ; methods ; Drug Implants ; Drugs, Chinese Herbal ; chemistry ; Rotation ; Temperature ; Time Factors

OBJECTIVETo provide a useful biological index for clinical diagnosis of Alzheimer's disease (AD) by determination the functional changes in the central cholinergic nerve and their effects on the peripheral lymphatic system.

METHODSThe learning and memory impairment model was established through intraventricular injecting quinolinic acid (QA) repeatedly.

RESULTSThere was a significant decline of cholineacetyltransferase (ChAT) in cerebral cortex and hippocampus after QA injection. The significantly lower binding activities of acetylcholine muscarinic (M) and nicotinic (N) cholinergic receptors in the hippocampus and cortex in the QA group were found as compared with the sham-operated group (P < 0.01). Similar changes were found in the binding activities of M-and N-receptors on spleen lymphocytes.

CONCLUSIONCertain lesion of the central nervous system can be reflected in peripheral spleen lymphocytes, which may be an important reference to diagnose the changes of the central nervous system.

Alzheimer Disease ; etiology ; Animals ; Brain ; drug effects ; Choline O-Acetyltransferase ; metabolism ; Disease Models, Animal ; Learning ; drug effects ; Male ; Memory ; drug effects ; Nicotine ; metabolism ; Quinolinic Acid ; toxicity ; Quinuclidinyl Benzilate ; metabolism ; Rats ; Rats, Wistar ; Receptors, Cholinergic ; analysis ; drug effects