Objective:To explore the effect of exercise on the memory of rats modeling vascular cognitive impairment (VCI) and also its effects on the hippocampal Sema3G/neuropilin-2 (Nrp2)/PlexinA4 signaling pathway.Methods:Eighteen male Sprague-Dawley rats were randomly divided into a sham-operated group, a model group, and an exercise group, each of 6. The model and exercise groups underwent VCI modeling via bilateral common carotid artery occlusion, while the sham-operated group received the same surgical procedure without vessel ligation or transection. Beginning forty-eight hours after the surgery, the exercise group carried out daily 30-minute treadmill training sessions, 5 days a week, for a total of 4 weeks, while the other two groups were placed on the same treadmill with it not in operation. After the intervention, cognitive functioning was assessed using the novel object recognition (NOR) test and a Y-maze test. Western blotting was employed to evaluate the expression of Sema3G, Nrp2, PlexinA4, and Rac1 in the hippocampus. Immunofluorescence staining was used to observe the distribution of Nrp2 and PlexinA4 in the hippocampus.Results:Compared with the model group, the exercise group exhibited significantly higher NOR indices during both the short-term and long-term memory testing phases after the intervention. Those rats also tended to have significantly longer total exploration times in the novel arm of the Y-maze test. The western blotting revealed that the expression levels of Sema3G, PlexinA4, and Rac1 in the hippocampus were significantly higher in the exercise group compared to the model group, on average. Immunofluorescence showed significantly increased PlexinA4 fluorescence intensity in the CA1, CA3, and dentate gyrus (DG) regions of the hippocampus, and significantly elevated Nrp2 fluorescence intensity in the CA3 region in the exercise group compared to the model group. The Pearson correlation coefficients for Nrp2/PlexinA4 co-localization in the CA1, CA3 and DG regions were significantly higher in the exercise group than in the model group.Conclusions:Exercise training significantly improves memory function in rats with VCI, and this effect may be associated with activation of the hippocampal Sema3G/Nrp2/PlexinA4 signaling pathway.

Objective To investigate the risk factors for the occurrence of short-term poor hearing recovery in patients with chronic suppurative otitis media(CSOM)underwent tympanoplasty under otoendoscope(TUO).Methods The clinical data of 260 patients with CSOM treated with TUO from January 2020 to October 2023 were retrospectively analyzed,the patients were followed up for 3 to 6 months after the operation,and were divided into the occurrence group(61 patients)and the non-occurrence group(199 patients)according to whether or not they experienced poor hearing recovery.The clinical data of patients in the two groups were compared,and the independent risk factors affecting the occurrence of poor hearing after TUO in CSOM patients were analyzed by Logistic regression model.Results The results of multivariate Logistic regression analysis showed that tensor tympani defects(OR=1.079,95％CI:1.054～1.105),tympanic mucosal lesions(OR=1.196,95％CI:1.076～1.330),auditory tube incompetence(OR=2.212,95％CI:1.339～3.654),auditory tube inflammation(OR=1.095,95％CI:1.047～1.146),tympanic mucosal fibrosis(OR=2.423,95％CI:1.402～4.186),and preoperative purulent secretion in the tympanic cavity(OR=1.070,95％CI:1.031～1.111)were the independent risk factors contributing to the development of poor hearing recovery after TUO in patients with CSOM(P＜0.05).Conclusion The occurrence of short-term poor hearing recovery after TUO in patients with CSOM is related to tensor tympani defects,tympanic mucosal lesions,auditory tube incompetence,auditory tube inflammation,tympanic mucosal fibrosis,and preoperative purulent secretions in the tympanic cavity,and clinically targeted preventive and curative measures may be given to postoperative patients with CSOM after TUO in order to avoid the occurrence of postoperative short-term poor hearing recovery.

Objective To investigate the risk factors for the occurrence of short-term poor hearing recovery in patients with chronic suppurative otitis media(CSOM)underwent tympanoplasty under otoendoscope(TUO).Methods The clinical data of 260 patients with CSOM treated with TUO from January 2020 to October 2023 were retrospectively analyzed,the patients were followed up for 3 to 6 months after the operation,and were divided into the occurrence group(61 patients)and the non-occurrence group(199 patients)according to whether or not they experienced poor hearing recovery.The clinical data of patients in the two groups were compared,and the independent risk factors affecting the occurrence of poor hearing after TUO in CSOM patients were analyzed by Logistic regression model.Results The results of multivariate Logistic regression analysis showed that tensor tympani defects(OR=1.079,95％CI:1.054～1.105),tympanic mucosal lesions(OR=1.196,95％CI:1.076～1.330),auditory tube incompetence(OR=2.212,95％CI:1.339～3.654),auditory tube inflammation(OR=1.095,95％CI:1.047～1.146),tympanic mucosal fibrosis(OR=2.423,95％CI:1.402～4.186),and preoperative purulent secretion in the tympanic cavity(OR=1.070,95％CI:1.031～1.111)were the independent risk factors contributing to the development of poor hearing recovery after TUO in patients with CSOM(P＜0.05).Conclusion The occurrence of short-term poor hearing recovery after TUO in patients with CSOM is related to tensor tympani defects,tympanic mucosal lesions,auditory tube incompetence,auditory tube inflammation,tympanic mucosal fibrosis,and preoperative purulent secretions in the tympanic cavity,and clinically targeted preventive and curative measures may be given to postoperative patients with CSOM after TUO in order to avoid the occurrence of postoperative short-term poor hearing recovery.

Objective:To explore the effect of exercise on the memory of rats modeling vascular cognitive impairment (VCI) and also its effects on the hippocampal Sema3G/neuropilin-2 (Nrp2)/PlexinA4 signaling pathway.Methods:Eighteen male Sprague-Dawley rats were randomly divided into a sham-operated group, a model group, and an exercise group, each of 6. The model and exercise groups underwent VCI modeling via bilateral common carotid artery occlusion, while the sham-operated group received the same surgical procedure without vessel ligation or transection. Beginning forty-eight hours after the surgery, the exercise group carried out daily 30-minute treadmill training sessions, 5 days a week, for a total of 4 weeks, while the other two groups were placed on the same treadmill with it not in operation. After the intervention, cognitive functioning was assessed using the novel object recognition (NOR) test and a Y-maze test. Western blotting was employed to evaluate the expression of Sema3G, Nrp2, PlexinA4, and Rac1 in the hippocampus. Immunofluorescence staining was used to observe the distribution of Nrp2 and PlexinA4 in the hippocampus.Results:Compared with the model group, the exercise group exhibited significantly higher NOR indices during both the short-term and long-term memory testing phases after the intervention. Those rats also tended to have significantly longer total exploration times in the novel arm of the Y-maze test. The western blotting revealed that the expression levels of Sema3G, PlexinA4, and Rac1 in the hippocampus were significantly higher in the exercise group compared to the model group, on average. Immunofluorescence showed significantly increased PlexinA4 fluorescence intensity in the CA1, CA3, and dentate gyrus (DG) regions of the hippocampus, and significantly elevated Nrp2 fluorescence intensity in the CA3 region in the exercise group compared to the model group. The Pearson correlation coefficients for Nrp2/PlexinA4 co-localization in the CA1, CA3 and DG regions were significantly higher in the exercise group than in the model group.Conclusions:Exercise training significantly improves memory function in rats with VCI, and this effect may be associated with activation of the hippocampal Sema3G/Nrp2/PlexinA4 signaling pathway.

Flow diverter (FD) devices are increasingly being widely used due to their higher safety and aneurysm occlusion rate compared to traditional coil embolization or stent-assisted coil embolization. However, in-stent stenosis (ISS) can occur after FD treatment, which may lead to hemiplegia, cerebral ischemia, and even endanger the life of patients. This article reviews the predictive factors of ISS after FD treatment for intracranial aneurysms, in order to provide reference for perioperative management of patients

Objective To explore CD59 expression on CD4+T cells in HIV infected patients and its relationship with apoptosis.Methods 12 HIV infected patients and 10 healthy donors were performed in this study.The PBMC(peripheral blood monocyte)were collected and cell surface cytokine were stained,and then were evaluated with the BD FACSCanto flow cytometry.The expression of CD59 on T lymphocyte subsets were analyzed by FACSDiva software,and the apoptosis rate of CD59+CD4+T cells and CD59-CD4+T cells in every group was analyzed respectively,then the results were compared between groups.Results Compared with healthy donor,the expression of CD59 on T cells in HIV infected patients was significantly hisher(t=5.198,P＜0.01),and the apoptosis rate of CD59+CD4+T cells had significantly higher(t=5.968,P＜0.01).The apoptosis rate of CD59-CD4+T cells was no difference between two groups (t=0.1353,P=0.8577).Condnsion HIV infection increase CD59 expression on CD4+T cells,and CD59+CD4+T cells were prone to apoptosis.

Objective To understand the virology test characteristics of hepatitis B virus (HBV) for discuss the relation of HBV genotype and HBeAg, anti-HBc-IgM, HBV DNA and disease progression. Methods Two hundred cases of hepatitis B were detected by the ELJSA assay with two pairs of semi-markers (HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc) and anti-HBc-IgM, using fluorescence quantitative polymerase chain reaction (FQ-PCR) for detecting HBV DNA, using monoclonal antibody ELISA method (mAbs ELISA) for HBV genotyping and analysis of test results. Results In 200 patients with hepatitis B, the HBV genotype detected in 179 cases (89.5%), B-type 121 cases(60.5%), C-type 58 cases (29.0% ). There had no relationship with HBeAg, anti-HBc-IgM, HBV DNA and genotype. B-type HBV prevalent in asymptomatic carriers (ASC) and chronic hepatitis B (mild);C-type common in patients with liver cirrhosis (LC) and chronic hepatitis B (severe). Conclusions HBV genotype in Shenzhen mainly is B-type, C-type second;mAbs ELISA assay with HBV genotype is specific, sensitive, simple and practical features, HBV replication strength has nothing to do with the virus genotype. HBV genotype and HBeAg, anti-HBc-IgM, HBV DNA testing may complement each other, with the clinical application value.