The Janus kinase/signal transducers and activators of transcription (JAK-STAT) control natural killer (NK) cells development and cytotoxic functions, however, whether long non-coding RNAs (lncRNAs) are involved in this pathway remains unknown. We found that miR155HG was elevated in activated NK cells and promoted their proliferation and effector functions in both NK92 and induced-pluripotent stem cells (iPSCs)-derived NK (iPSC-NK) cells, without reliance on its derived miR-155 and micropeptide P155. Mechanistically, miR155HG bound to miR-6756 and relieved its repression of JAK3 expression, thereby promoting the JAK-STAT pathway and enhancing NK cell proliferation and function. Further investigations disclosed that upon cytokine stimulation, STAT3 directly interacts with miR155HG promoter and induces miR155HG transcription. Collectively, we identify a miR155HG-mediated positive feedback loop of the JAK-STAT signaling. Our study will also provide a power target regarding miR155HG for improving NK cell generation and effector function in the field of NK cell adoptive transfer therapy against cancer, especially iPSC-derived NK cells.

Objective To observe the disinfection effect of hypobromic acid on the oral comprehensive treatment table water system(DUWLs).Methods The research equipment was selected from hospital DUWLs,including 20 sets of three purpose spray guns,dental mobile phones,and water cup injectors.The three types of equipment were arranged in numbers 1-20,with devices 1-10 included in the experimental group and devices 11-20 included in the control group.The control group was not disinfected with DUWLs,while the experimental group was disinfected with hypobromic acid.Before daily diagnosis and treatment,water samples were collected for 3 minutes after rinsing.The collection time was on the 0th,1st,2nd,3rd,4th,5th,8th,10th,15th,20th,25th,and 30th days,with a total of 12 samples taken.The generalized estimation equation was constructed to compare the colony count of the cell phone jack,the water cup outlet and the three gun sockets of the two groups at different time points.The qualified rate of DUWLs water samples was compared.Results The generalized estimation equation was constructed to test the significance of the fixed effect of intercept,group,time point,group time point for the bacterial count of the two groups of mobile phone jack parts,the water cup outlet parts and the three gun jack parts.The variation difference between the two groups of data at different time points was statistically significant(P<0.05).Before the first treatment,the number of colonies in the experimental group was 16 800 more than that in the control group(P<0.05).On the 30th day,the bacterial count in the control group decreased by 29 000 compared to before the first consultation.On the 30th day,the decrease in bacterial count in the experimental group was 35 250 more than that in the control group(P<0.05).There was no statistical significant difference in the number of colonies at the cell phone socket between the two groups before the first diagnosis(P>0.05).On the 30th day,the bacterial count in the control group decreased by 86 900 compared to before the first consultation.On the 30th day,the decrease in bacterial count in the experimental group was 16 350 more than that in the control group(P>0.05).There was no statistical significant difference between the two groups in the number of colonies in the outlet of the water cup before the first diagnosis(P>0.05).On the 30th day,the bacterial count in the control group decreased by 25 550 compared to before the first consultation.On the 30th day,the decrease in bacterial count in the experimental group was 11 800 more than that in the control group(P<0.05).The pass rate of mobile phone jack,water cup outlet and gun jack in the experimental group was higher than that in the control group before diagnosis and treatment and after rinsing for 3 minutes,and the difference was statistically significant(P<0.05).Conclusion Using hypobromic acid for DUWLs disinfection can reduce bacterial count and improve the qualified rate of water samples.

Hela is the cell line of adenocarcinoma of the uterine cervix, and human papillomavirus (HPV) 18 shows positive. We delivered siRNA with target specifically to HPV18 E7 mRNA into nude mice Hela tumor xenografts by nanopatch to inhibit the HPV gene expression, and further to study the superiority, the best action time and concentration of siRNA of using nanopatch to transfer siRNA in vivo. We designed siRNA that target specifically to HPV18 E7 mRNA (siE7) and checked the effect of siE7 in vitro. Tumor xenografts were transfected with siE7 and GenEscort III by nanopatch. Expression of HPV18 E7 mRNA and protein were detected 0 hours, 24 hours, 48 hours, 72 hours after transfection with PT-PCR and Western blot, and the best action time was analyzed using nanopatch to thansfect siRNA in vivo. We transfected GenEscort III and siE7 of Different concentration into tumor xenografts respectively by nanopatch and intraperitoneal injection. Expression of HPV18 E7 mRNA and protein was detected 72 hours after transfection by PT-PCR and Western blot, to analyze the best action concentration of siRNA and the superiority of using nanopatch to thansfect siRNA in vivo. The results proved that SiE7was efficient to inhibit expression of HPV18 E7 mRNA and to advance Hela apoptosis in vitro. SiE7 transfected by nanopatch into xenografts could inhibit effectively expression of HPV18 E7 mRNA and protein. The best action time and concentration of siRNA of using nanopatch to thansfect siRNA in vivo are 72 hour post-transfection and 2 micromol/L siE7. To compare intraperitoneal injection in delivering siRNA in vivo, the effect of nanopatch is very predominant. It can be well concluded that Nanopatch can effectively transfer siRNA in vivo, which can effectively inhibit the HPV gene expression.
Animals ; Apoptosis ; DNA-Binding Proteins ; genetics ; Female ; Gene Expression Regulation, Viral ; HeLa Cells ; Humans ; Mice ; Mice, Nude ; Nanostructures ; administration & dosage ; Oncogene Proteins, Viral ; genetics ; Papillomaviridae ; genetics ; RNA, Small Interfering ; administration & dosage ; Transfection ; Uterine Cervical Neoplasms ; therapy ; Xenograft Model Antitumor Assays